Ophioluxin, a convulxin-like C-type lectin from Ophiophagus hannah (King cobra) is a powerful platelet activator via glycoprotein VI

Ophioluxin, a potent platelet agonist, was purified from the venom of Ophiophagus hannah (King cobra). Under nonreducing conditions it has a mass of 85 kDa, similar to convulxin, and on reduction gives two subunits with masses of 16 and 17 kDa, slightly larger than those of convulxin. The N-terminal sequences of both subunits are very similar to those of convulxin and other C-type lectins. Ophioluxin induces a pattern of tyrosine-phosphorylated proteins in platelets like that caused by convulxin, when using appropriate concentrations based on aggregation response, because it is about 2-4 times more powerful as agonist than the latter. Ophioluxin and convulxin induce [Ca(2+)](i) elevation both in platelets and in Dami megakaryocytic cells, and each of these C-type lectins desensitizes responses to the other. Convulxin agglutinates fixed platelets at 2 microg/ml, whereas ophioluxin does not, even at 80 microg/ml. Ophioluxin resembles convulxin more than echicetin or alboaggregin B because polyclonal anti-ophioluxin antibodies recognize both ophioluxin and convulxin, but not echicetin, and platelets adhere to and spread on ophioluxin- or convulxin-precoated surfaces in the same way that is clearly different from their behavior on an alboaggregin B surface. Immobilized ophioluxin was used to isolate the glycoprotein VI-Fcgamma complex from resting platelets, which also contained Fyn, Lyn, Syk, LAT, and SLP76. Ophioluxin is the first multiheterodimeric, convulxin-like snake C-type lectin, as well as the first platelet agonist, to be described from the Elapidae snake family.

Projektvorstellung: Nachwuchsgruppe „Eigentums- und Besitzrechte von Frauen in der Rechtspraxis des Alten Reiches (1648-1806)“ an der Friedrich-Schiller-Universität Jena

Kritische Beleuchtung der in den Jahren 1831 und 1832 in Deutschland vorgekommenen ständischen Verhandelungen über die Emancipation der Juden

von Gabriel Riesser

Die Christen und die Juden oder Richterspruch der Vernunft über das Leben, Dichten und Trachten der Juden : mit Nachrichten vom großen Sanhedrin, welcher auf Befehl Napoleons in Paris 1806 gehalten wurde

vom Weissen-Becker

Civil disabilities of the Jews : eine 1831 veröff. Abhandlung

Thomas Babington Macaulay. Hrsg. u. m. Anm. vers. von F. Fischer

Festschrift zur Hundertjahrfeier des Vereins 1806 - 1906 : Geschichte der Chewra Kadischa, Israeltischer Verein für Krankenpflege, Bestattungswesen und religiöse Belehrung in München

von H. Ehrentreu