956 resultados para DIRECT-CONTACT TEST


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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The objective of this study was to assess the bone repair process of crystallized Biosilicate in surgically created defects on rats' calvaria. This biomaterial was recently developed for odontological use. We used fifteen rats (rattus norvegicus albinus, Wistar), and two 5 mm surgical defects were performed on each of them; the defects were made with trephine drill on the calvarium region prior to the biomaterial placement. Groups were divided as follows: Group 1-defect filled with clot; Group 2-defect filled with crystallized Biosilicate. After 7, 14 and 28 days the animals were killed, the parts were retrieved and slides were prepared for histological studies. Bone formation was satisfactory in all groups, with direct contact between biomaterial surface and bone and absence of infection signs. The 28 days periods showed better results, and statistically significant difference between Clot Group (90.2 %) and Biosilicate (58 %; p = 0.002) was seen, regarding presence of bone tissue on the surgical defects. Our study revealed that defects filled with clot present better results on bone formation compared to crystallized Biosilicate, which is considered a biocompatible material with favorable osteoconductive properties.

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Pós-graduação em Agronomia (Proteção de Plantas) - FCA

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Pós-graduação em Agronomia - FEIS

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The purpose of this study was to evaluate the possibility to obtaining guided bone regeneration utilizing a nonporous PTFE barrier in the osseointegrated implants, protruding from the bone level of the rabbit tíbia. The histologic characteristics of the interface between titanium implants, one group titanium-plasma coated, another group with acid-treated surfaces and the regenerated bone were also studied Twenty Screw-Vent implants were placed in tibias of five rabbits, two at the right side and two at the left side, protruding 3 mm from the bone level, to create a horizontal bone defect. ln the experimental side, the implants and adjacent bone were protected with a nonporous PTFE barrier. Histologic analysis after three months showed that all implants were in direct contact with the bane. Histologic measurements showed an average gain in bone height of the 2.15 and 2.42 mm for the barrier group and 1.95 and 0.43 mm for the control defects, in the titanium plasma-spray and acid-treated implant surfaces, respectively. The results suggest that the placement of implants protruding 3 mm from crestal bone defects may result in vertical bone augmentation and the regenerated bone is able to osseointegrate implants. lt seems to be critical the use of the PTFE barrier when acid-treated surface implants are inserted

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Canine transmissible venereal tumour (CTVT) is a neoplasm transmitted among healthy dogs by direct contact with injured skin and/or mucous tissue. This study aimed to identify the TP53 gene, messenger RNA (mRNA) as well as the expression of p53, Bcl-2 and p63 proteins in histological sections of 13 CTVT samples at different stages of evolution. The in situ hybridization (ISH) and in situ reverse transcriptase polymerase chain reaction (RT-PCR) assays were used, which showed the DNA homologous to TP53 and its respective mRNA in 92.3% of the samples. We detected p53, p63 and Bcl-2 proteins in most of the cell samples in different grades of intensity. In addition, 46% of the samples were in the progressive and 54% in the regression phase. This is the first description of these proteins and a detailed study of their role in CTVT cells needs to be addressed in or to verify how these cells undergo apoptosis.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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When we discuss environmental issues, research shows that individuals do not seem to present a genuine perception of the natural environment, which can be justified by the current context of modernity, in which the contact of many people with a natural environment is scarce and in front of this, build a perception of environment through intermediaries, such as classes, books, magazines or television. The Universidade Estadual Paulista Júlio de Mesquita Filho, located in Bauru - SP, allows its goers a direct contact with the Cerrado, since part of this biome reserve is located in the institution. This paper reports a research on perceptions related to the Cerrado, conducted with students of the first and last year of Graduation in Engineering. The aim was to investigate whether there are differences in perceptions found in beginners and senior students who attended about five years of disciplines on a campus present in the Cerrado area.

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This paper presents one proposal of the energy management's model system using photovoltaic panels. The module proposed seeks to monitor photovoltaic panels, which have intermittency in power generation caused by environmental or load conditions, in order to control the coupling between the panel and the load - through the charge controller, of aiming that the panel's operation will be always on the maximum power transfer point as possible. For this, it used the maximum power point tracking technique - MPPT, implemented in LabVIEW software, also utilizing the data acquisition card NI myDAQ. In addition, it was implemented the controller access remote module, from the sharing of network data, so the panels performance can be through a tablet, monitored and controlled with no need for direct contact with the Supervisory server

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To determine the ability of experimentally inoculated white-tailed deer (Odocoileus virginianus) to transmit Mycobacterium bovis to naive deer through the sharing of feed, four deer were intratonsillarly inoculated with 4x105 colony-forming units of M. bovis. On a daily basis, feed not consumed by inoculated deer after approximately 8 hr was offered to four naıve deer maintained in a separate pen, where direct contact, aerosol transmission, or transmission through personnel were prevented. After 150 days, naıve deer were euthanized and examined. All naıve deer had lesions consistent with tuberculosis and M. bovis was isolated from various tissues. The most commonly affected tissues were lung, tracheobronchial lymph nodes, and mediastinal lymph nodes. This study demonstrates the potential for indirect transmission of M. bovis through the sharing of feed. Intentional or unintentional feeding of deer by wildlife or agricultural interests in regions where M. bovis infection is endemic should be avoided because both direct and indirect transmission through sharing of feed are enhanced.

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Bovine tuberculosis (TB) is an infectious and communicable granulomatous disease caused by the acidfast bacilli bacteria of Mycobacterium bovis (M. bovis). It is commonly a chronic, debilitating disease, but occasionally may assume an acute, rapidly progressive course. M. bovisis a widespread zoonosis that is global in magnitude and affects nearly all species of vertebrates (cattle, sheep, goats, pigs, bison, buffalo, and camelids.) Disease is spread by direct contact, inhalation of infected droplets expelled from infected lungs, and ingestion of contaminated feed or milk. In most countries, TB is a notifiable disease. Overall, TB has an important world-wide impact on animal industries and human health. Control measures are based on prevention and eradication. Surveillance is a key element for management of preventions and control programs. Surveillance for TB serves the purpose of enabling Veterinary Services to obtain an accurate picture of the scope of the disease in the US livestock populations; in the event of a disease outbreak, the course TB follows in livestock and wildlife populations for a given area over time; and permits timely intervention if the trend observed deviates from what is expected.