Results of MDR-1 vector modification trial indicate that granulocyte/macrophage colony-forming unit cells do not contribute to posttransplant hematopoietic recovery following intensive systemic therapy

To formally test the hypothesis that the granulocyte/macrophage colony-forming unit (GM-CFU) cells can contribute to early hematopoietic reconstitution immediately after transplant, the frequency of genetically modified GM-CFU after retroviral vector transduction was measured by a quantitative in situ polymerase chain reaction (PCR), which is specific for the multidrug resistance-1 (MDR-1) vector, and by a quantitative GM-CFU methylcellulose plating assay. The results of this analysis showed no difference between the transduction frequency in the products of two different transduction protocols: “suspension transduction” and “stromal growth factor transduction.” However, when an analysis of the frequency of cells positive for the retroviral MDR-1 vector posttransplantation was carried out, 0 of 10 patients transplanted with cells transduced by the suspension method were positive for the vector MDR-1 posttransplant, whereas 5 of 8 patients transplanted with the cells transduced by the stromal growth factor method were positive for the MDR-1 vector transcription unit by in situ or in solution PCR assay (a difference that is significant at the P = 0.0065 level by the Fisher exact test). These data suggest that only very small subsets of the GM-CFU fraction of myeloid cells, if any, contribute to the repopulation of the hematopoietic tissues that occurs following intensive systemic therapy and transplantation of autologous hematopoietic cells.

Letter from John Avery to Cotton Tufts, 1790 April 9

A handwritten invitation from John Avery to Cotton Tufts for a meeting of the Harvard Board of Overseers' "Committee to enquire into the state of the College" on April 13, 1790.

Commonplace book of Moses Appleton, 1791-1815 (inclusive)

Volume containing medicinal recipes, medical notes, poetry, and obituaries written by Dr. Moses Appleton (1773-1849). Many of the recipes were copied from medical texts or other publications. His "cure for the dropsy," taken from the New York Herald, contained stale cider, parsley, horseradish, oxymel squills (sea onion in honey), and juniper berries. For diarrhea, he prescribed a blackberry syrup. Several entries indicate Appleton practiced Thomsonian medicine, an alternative system based on use of botanicals. The medical notes include an account of his treatment of a man with smallpox in 1815, and entries on patients he inoculated with cowpox matter. Another entry dated in 1796 provides instructions from the Massachusetts Humane Society for "treatment to be used with persons apparently dead from drowning," which included blowing tobacco smoke in the victim's lungs and applying warm blankets for several hours. Appleton adds a note questioning whether or not the lungs also should be "often artificially inflated." There is additionally a history of prominent physicians dating from ancient Greece.

Hādhā kitāb al-Fīrūzaj: Sharḥ al-Unmūdhaj

li-Muḥammad ʻĪsá ʻAskar.

Kitāb al-Jaysh al-kafīl bi-akhdh al-thaʼr mimman salla ʻalá al-Shaykh al-Tijānī sayf al-inkār

taʼlīf Muḥammad ibn Muḥammad al-Ṣaghīr al-Shinjīṭī al-Tashītī ; bi-taṣḥīḥ wa-muqābalat Ab�� ʻAbd Allāh Muḥammad Gannūn.

Kitāb Shams al-taḥqīq wa-ʻurwat ahl al-tawfīq

lil-Imām Aḥmad ibn Sharqāwī. Wa-yalīhā kitāb Naṣīḥat al-dhākirīn wa-irghām al-mukābirīn / lil-Ustādh al-madhkūr. Muṭarrizan hāmishahā bi-al-risālah al-musammāh bi-al-Asinnah al-faʻʻālah fī akb��d man ankara ʻalá al-ustādh marartu ʻalá al-jalālah / lil-ʻAllāmah al-Muḥaqqiq Mawlānā al-Shaykh Muḥammad al-Miṣrī al-Jirjāwī.

ʻIlm al-Kitāb

Khavājah Mīr al-Muḥammadī al-mutakhalliṣ bi-Dard.

Nawādir Mawlānā al-ʻālim al-ʻallāmah al-ḥibr al-baḥr al-qudwah al-fahhāmah al-ustādh Aḥmad Shihāb al-Dīn al-Qalyūb��

Date and imprint taken from colophon.

Kitāb Sharḥ Ibn Qāsim al-ʻAbbādī ʻalá Sharḥ al-Waraqāt lil-Shaykh Jalāl al-Dīn al-Maḥallī : manuscript, 1649

According to the colophon (f. 60r), copy completed on 16 Rajab 1059 AH [July, 25, 1649 AD].

Kitāb al-Wāfī ʻalá madhhab al-Imām Ab�� Ḥanīfah : manuscript, 1334

According to the colophon (f. 245r), copy completed on 20 Ramaḍān 734 AH [May 24, 1334 AD].

[ B��î:b��ḍhāî:jo:saṁvad:tathā:gûgrījā:gīnān:10]

Pīr Imām Shāh, B��î B��ḍhāî, Gur Imām Shāh..

Britain’s Future in Europe: The known Plan A to remain or the unknown Plan B to leave. CEPS Paperback 2nd Ed., March 2016

Following the agreement made by Prime Minister David Cameron with the EU on 18-19 February 2016, the day for the referendum for the UK to remain in or leave the EU is set for 23 June 2016. This will be the most important decision taken by the British people in half a century, and whose consequences will live on for another half century. The first edition of this book, published in March 2015, laid the foundations for any objective assessment of the workings of the EU and the UK’s place in it. It was widely acclaimed and rated as “a myth-breaking exercise of the best kind”. This second edition adds a substantial new chapter following Cameron’s agreement with the EU and announcement of the referendum. It reviews both the ‘Plan A’, namely the status quo for the UK in the EU as amended by the new agreement, and three variants of a ‘Plan B��� for secession. The key point is that the ‘leave’ camp have not done their homework or ‘due diligence’ to specify the post-secession scenario, or how the British government would face up to the challenges that this would bring. The authors therefore do the ‘leave’ camp’s homework for them, setting out three Plan Bs more concretely and in more depth than the ‘leave’ camp have been able or wanted to do, or any other source has done. The book is therefore unique and essential reading for anyone concerned with the fateful choice that lies soon ahead.