Les ésclaves chrétiens,

Mode of access: Internet.

Cataloging rules, with explanations and illustrations,

A revision of the preliminary edition published by The New York state library in 1914 as Library school bulletin 36. Based on The A.L.A. catalog rules.

A guide to Federal housing programs for the mentally disabled /

Prepared under contract no. 278-75-0030(MH).

Réfutation des allégations contre l'administration du Muséum d'histoire naturelle : proférées a la Tribune du Corps législatif, dans la séance du 19 juin 1862.

Mode of access: Internet.

From fifteen to twenty-five; a book for young men,

Mode of access: Internet.

Histoire de la maison de Rochechouart /

Includes bibliographical references.

Population ageing and the implications for finanical markets

How will financial institutions respond to the transactions and asset management needs of both the ageing population and their carers? The ageing of the population has generated increased interest from both government and business, including banking and financial services, in the sorts of services that will be required by older people, and how their money and property will be managed. This article examines the trends and implications for banking practice of this increasing population of customers and their carers.

Sweet potato in Oceania: a reappraisal

allard's introductory chapter reviews the lively debate concerning the introduction of sweet potato into Oceania and its role in debates concerning population growth, population density, and their relationship to agricultural intensification and socio- economic and political change, particularly in New Guinea. Other forms of proxy data include archaeological evidence for cropping and agricultural technology (Coil and Kirch); temporal data indicative of shifts in landscape use and changing agricultural practices (Bayliss-Smith et al.; Haberle and Atkin; Wallin et al.); and data from legends, ethnohistoric documents, and ethnographic studies providing evidence for the timing of the introduction, and the importance of the crop in various Oceanic societies (Allen; Dunis; Wallin et al.).

Critical reflections on international collaborative research

The excitement and challenge of undertaking research is an integral part of an academic staff member’s role. There are a multitude of reasons which encourage academics to undertake collaborative research. These range from the enthusiasm that arises from particular discipline interests, through to the pressure from tertiary contexts to be actively engaged in research and to produce research outputs. This paper uses the experiences of an international academic research team to explore the nature of the collaborative academic research process, including the perils and pitfalls, as well as the joys and enthusiasms. The three researchers are convinced that there are many positives to be gained from international collaboration. By critically reflecting on the dynamics of the research process employed by the tri-national team, (as against the research project itself), and identifying ‘lessons learned’ by the researchers themselves, suggestions for productive and enjoyable research relationships are offered.

Exceptional overproduction of a functional human membrane protein

Eukaryotic-especially human-membrane protein overproduction remains a major challenge in biochemistry. Heterologously overproduced and purified proteins provide a starting point for further biochemical, biophysical and structural studies, and the lack of sufficient quantities of functional membrane proteins is frequently a bottleneck hindering this. Here, we report exceptionally high production levels of a correctly folded and crystallisable recombinant human integral membrane protein in its active form; human aquaporin 1 (hAQP1) has been heterologously produced in the membranes of the methylotrophic yeast Pichia pastoris. After solubilisation and a two step purification procedure, at least 90 mg hAQP1 per liter of culture is obtained. Water channel activity of this purified hAQP1 was verified by reconstitution into proteoliposomes and performing stopped-flow vesicle shrinkage measurements. Mass spectrometry confirmed the identity of hAQP1 in crude membrane preparations, and also from purified protein reconstituted into proteoliposomes. Furthermore, crystallisation screens yielded diffraction quality crystals of untagged recombinant hAQP1. This study illustrates the power of the yeast P. pastoris as a host to produce exceptionally high yields of a functionally active, human integral membrane protein for subsequent functional and structural characterization. © 2007 Elsevier Inc. All rights reserved.