Efeito da própolis sobre a produção de citocinas por camundongos submetidos a estresse crônico e desafiados com melanoma

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características florais e dependência por polinizadores de cinco cultivares de pepino e manejo de colméias em estufas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Toxicidade de inseticidas para abelhas Apis mellifera L

Pós-graduação em Zootecnia - FMVZ

Efeitos de abelhas na frutificação e qualidade de melancia (cv. Crimson Sweet) na região Central do estado do Piauí

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

A case of Tinea nigra associated to a bite from a European rabbit (Oryctolagus cuniculus, Leporidae): the role of dermoscopy in diagnosis

We report a case of Tinea nigra in an adolescent living in Itapema, Santa Catarina, Brazil, who presented a hyperchromic macule on the palm of the left hand, close to another erythematous macule caused by a rabbit bite. The patient received guidance on accidents and animal bites and evolved well treated with topical butenafine for the dermatomycosis. The authors also highlight the efficacy of the dermoscopic exam in diagnosing Tinea nigra with animal bite lesions and other traumas.

An outbreak of Portuguese man-of-war (Physalia physalis - Linnaeus, 1758) envenoming in Southeastern Brazil

Introduction Portuguese man-of-war, Physalia physalis (Linnaeus, 1758), are cnidarians capable of discharging intracellular organelles filled with venom, resulting in severe envenomation in humans. Methods We report the clinical and therapeutic aspects of 331 accidents involving Portuguese man-of-war in an outbreak on the coast of the State of São Paulo, Brazil. Results The clinical manifestations of envenomation were rare and mild and mostly local, systemic reactions; there was a low rate of late complications. Conclusions The consequences of envenomation were of moderate severity, and first aid measures were effective in controlling the pain. Outbreaks of accidents involving Portuguese man-of-war occur periodically in the area without a clear explanation.

Clinical manifestations and experimental studies on the spine extract of the toadfish Porichthys porosissimus

Toadfish are fish from the family Batrachoididae that are found in marine and brackish environment around the world. Among the toadfish, Porichthys genus is very common, where Porichthys porosissimus, also called Atlantic Midshipman is found in Southwest Atlantic, from Rio de Janeiro, Brazil to eastern Argentina. There was no consensus about the classification of the genus Porichthys as venomous fish because so far there are no published Studies regarding human envenomations and/or toxic activities induced in animal models. Herein, we report two conclusive envenoming in human beings caused by P porosissimus spines, with clear signs and symptoms that were very important for the development of our experimental studies. We demonstrated that the P. porosissimus spine extract, now venom, can induce nociceptive and edematogenic responses in mice as well an induction of an inflammatory response elicited by intravital microscopy and leukocyte migration. Finally, we identified in the P. porosissimus spine extract, through analysis by mass spectrometry, the presence of proteins previously detected in the venoms of other fish species and other venomous animals. We believe that based on our studies we will dismiss the non-venomous nature of this fish and clarify this issue. (C) 2014 Elsevier Ltd. All rights reserved.

Increased brood viability and longer lifespan of honeybees selected for propolis production

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Honey bee lines selected for high propolis production also have superior hygienic behavior and increased honey and pollen stores

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Clinical aspects of envenomation caused by Tityus obscurus (Gervais, 1843) in two distinct regions of Para state, Brazilian Amazon basin: a prospective case series

Background: Scorpion envenomations are a major public health problem in Brazil, whose most dangerous cases are attributable to the genus Tityus. This study was designed to compare the clinical and demographic features of envenomations by Tityus obscurus in two areas of the state of Para located in the Amazon basin. Were compared demographic findings, local and systemic signs and symptoms of human envenomations caused by T. obscurus that occurred in western and eastern areas of the state.Results: Forty-eight patients with confirmed envenomation by T. obscurus were evaluated from January 2008 to July 2011. Most of them came from the eastern region, where male and female patients were present in similar numbers, while males predominated in the west. Median age groups were also similar in both areas. Most scorpion stings took place during the day and occurred significantly more frequently on the upper limbs. The time between the sting and admission to the health center was less than three hours in both areas. Most eastern patients had local manifestations while in the west, systemic manifestations predominated. Local symptoms were similar in both areas, but systemic signs and symptoms were more common in the west. Symptoms frequently observed at the sting site were local and radiating pain, paresthesia, edema, erythema, sweating, piloerection and burning. The systemic manifestations were significantly higher in patients from the west. Futhermore, neurological symptoms such as general paresthesia, ataxia, dysarthria, myoclonus, dysmetria, and electric shock-like sensations throughout the body were reported only by patients from the west.Conclusion: The present study shows that two regions of Para state differ in the clinical manifestations and severity of confirmed envenomation by T. obscurus which suggests a toxicity variation resulting from the diversity of T. obscurus venom in different areas of the Brazilian Amazon basin, and that T. serrulatus antivenom can be successfully used against T. obscurus.

Estudo de repelência com diversos produtos de origem natural em operárias de Apis mellifera em semi-campo

Accidents involving insects of the Hymenoptera order occur very often with both human beings and domestic pets and, in Brazil, they include aggravated cases with Africanized bees (Apis mellifera). The aggravation of deforestation and the lack of awareness regarding the subject are factors that contribute to the rise of the number of bees in the urban environment. This fact has been causing several derangements among the population because, once these insects are bothered, they become very aggressive. Considering the risks to population and the great amount of accidents that could be avoided, the development of researches with the goal of determining repelling substances is rather important. Therefore, this research evaluated the repelling action of essential natural oils obtained from rosemary (Rosmarinus oficinalis), lemongrass (Cymbopogon citratus), thyme (Thymus vulgaris), cedar (Juniperus virginiana), clove (Syzygium aromaticum) and mint (Mentha piperita) on A. mellifera Africanized worker bees in both semi-field and aggressiveness tests. Among the evaluated composites, the lemongrass, mint and clove essential natural oils presented a grater repelling effect, inhibiting the bees’ visitation to the managed feeders almost completely. The cedar essential natural oil was the least effective composite, and the rest of the tested oils presented satisfactory repellency, which became less effective over time, according to non-parametric Mann-Whitney test. However, further tests showed that only the lemongrass essential natural oil caused a less aggressive response from the bees, which can confirm the repelling power of this composite. This way, according to the results obtained through this research, lemongrass presents a greater potential to the development of effective repelling formulas against Africanized bees (Apis mellifera)

Análises morfológica e histoquímicas do ventrículo e do cérebro de operárias recém emergidas de Apis Mellifera L. (Hymenoptera, Apidae) tratadas com Thiamethoxam

Apis mellifera honeybees are social insects of economic importance, by providing honeybee products, and by the pollination of natural areas of vegetation or agricultural areas. The constant use of pesticides, including the thiamethoxam, which is an insecticide belonging to the class of neonicotinoids with neurotoxic action, is subjecting pollinators to situations of severe stress, which has been evidenced by the decrease in the density of honeybees in many parts of the world. By these considerations, the present study aimed to assess the acute toxicity of thiamethoxam for newly emerged workers of A. mellifera, and to investigate the effect of sublethal doses of this insecticide on the survival time and its cytotoxicity to the brain and midgut to the honeybees. It was established the value of the lethal concentration 50 (LC50) equal to 4.28 ng thiamethoxam/μL of food and from it, the sublethal concentrations of 0.428 ng thiamethoxam/μL (CL50/10) and 0.0428 ng thiamethoxam/μL (CL50/100), which were used in bioassays of intoxication of the honeybees. After the bioassays the bees were dissected and the brain and midguts were collected to analyze possible morphological (staining with Hematoxylin-Eosin) and histochemical alterations (Xylidine Ponceau technique, and Feulgen and PAS reactions) caused by exposure to thiamethoxam, and to calculate the lethal time (LT50) for the workers. The duration of the bioassays was 8 days after beginning of feeding. The results obtained showed that the thiamethoxam is toxic to newly-emerged workers of A. mellifera, causing changes in survival time of individuals. This study also shown that the thiamethoxam causes morphological and histochemical alterations on the midgut and brain of workers. These alterations may be reflected in physiological and behavioral changes that can modify the operation of the colony

Desenvolvimento de uma plataforma analítica para aquisição de imagens moleculares em tecidos animais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação dos efeitos de concentrações subletais de dimetoato no cérebro e ventrículo de Apis mellifera africanizada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Report of 15 injuries caused by lionfish (pterois volitans) in aquarists in Brazil: a critical assessment of the severity of envenomations

Lionfish are venomous fish that belong to the Scorpaenidae family. Individuals of this family and those of the Synanceiidae family comprise most of the existing venomous fish in the world. Lionfish are originally found in the Indo-Pacific, but they have received special attention in the last years for their dissemination in the Atlantic Ocean, with the emergence of large populations in the USA, Caribbean and South America. Because of its beauty, this fish has always been present in private and commercial aquariums around the world. Herein, we describe 15 envenomations in aquarists registered in a period of eighteen years (1997-2014). The stings caused excruciating pain and marked inflammation, with local erythema, edema, heat, paleness and cyanosis. In one case, it was possible to observe vesicles and blisters. There were no skin necroses or marked systemic manifestations. We discuss the possible coming of the fish to South America and the circumstances and clinical impact of the envenomations.