993 resultados para 382.098
Resumo:
,The molecular dynamics research of the core domain of p53 protein crystal structure shows that besides the stability in biochemistry this domain also shows a high stability in molecular mechanics. Based on that work, the residue R249 was substituted with amino acids Gly and Ser respectively, and molecular dynamics researches were performed separately. The results show that these substitutions cause a relax tendency between loop2 and 3 domains, leading to an alteration of the whole conformation of p53 core domain and ruining its stability. The results visually explains the mechanism of p53 changes in immunological and biochemical reactions, which are caused by 249 residue substitutions from 3-D structure variations.
Resumo:
Despite the evolutional distance between wasp and amphibian, vespid chemotactic peptide (VCP), an important component of wasp venom, are found sharing remarkable similarities with the temporin antimicrobial peptides (AMPs) from Ranid frog, Amolops loloens
Resumo:
Zinc oxide (ZnO) thin films were deposited at high rates ( > 50 nm min-1) using a unique technique known as high target utilisation sputtering (HiTUS). The films obtained possess good crystallographic orientation, low surface roughness, very low stress and excellent piezoelectric properties. We have utilised the films to develop highly sensitive biosensors based on thickness longitudinal mode (TLM) thin film bulk acoustic resonators (FBARs). The FBARs have the fundamental TLM at a frequency near 1.5 GHz and quality factor Q higher than 1,000, which is one of the largest values ever reported for ZnO-based FBARs. Bovine Serum Albumin (BSA) solutions with different concentrations were placed on the top of different sets of identical FBARs and their responses to mass-loading from physically adsorbed protein coatings were investigated. These resonators demonstrated a high sensitivity and thus have a great potential as gravimetric sensors for biomedical applications. © 2011 Inderscience Enterprises Ltd.
Resumo:
One of the most promising prophylactic agents being tested to control Penaeus monodon larval diseases is furanace (6-hydroxymethyl-2 2(5-nitro-2-furyl) vinyl pyridine). To evaluate further its suitability as a chemotherapeutic agent, its effects on the population growth of Chaetoceros calcitrans, the diatom used as feed for the zoeal stages, was examined. Chaetoceros populations of uniform density (initial density in all runs: 130-141x10 SUP-3 cells /ml) were placed in nine white, circular (382 sq cm), plastic basins. The physio-chemical characteristics of the culture water were as follows: salinity, 28 . 5-30 . 0 ppt; pH, 8 . 62-8 . 72; temperature, 23-25 . 5 degree C; dissolved oxygen, 7 . 1-9 . 3 ppm; nitrate, 0 . 03-0 . 07 ppm; and ammonia, 0 . 005-0 . 03 ppm. Preweighed furanace granules were dissolved in the culture water, with resulting concentrations of 1 and 2 mg/l (3 replicates each). A set of replicates without furanace served as the control. Population counts of the diatom were taken after 2, 4, 6, 8, and 10 hr exposures. After 4 hr, the population decreased in all three levels. The population in 2 mg/l furanace showed the lowest count and that in control the highest. The population means are not statistically different from one another. The results of the study show that the furanace causes reductions in Chaetoceros population at all durations of exposure.
Resumo:
综述了天然产物中对H IV 21 逆转录酶具有抑制活性的化合物的来源和分类。重点介绍了几种H IV 21 非核 苷类逆转录酶抑制剂(NNRT Is) 的性质、特点、抗H IV 21 活性及其构效关系。从天然资源和传统中草药发现具有新 结构、新作用机制的天然NNRT Is 是开发研制抗H IV 21 药物的一条极有希望的途径。
Resumo:
For a period of one year beginning December 1977, drift card experiments were conducted off the western and southern coasts of Panay Island to determine the surface currents in the area. Of a total 2,384 drift cards released during the study, 382 (16.02%) were recovered, 92% of them within 30 days following dispatch. The surface currents in the study area are strongly influenced, in direction and speed, by the prevailing monsoon winds. During the NE monsoon period, the surface currents move away from the coast; during the SW monsoon, toward and/or parallel to the coast. Based on the results, the probable movement and transport of milkfish (Chanos chanos) eggs and larvae from the spawning ground to the fry collection ground are also discussed.
Resumo:
Enfuvirtide (ENF) is currently the only FDA approved HIV fusion inhibitor in clinical use. Searching for more drugs in this category with higher efficacy and lower toxicity seems to be a logical next step. In line with this objective, a synthetic peptide
Resumo:
The effects of morphine on hippocampal sensory gating (N40) during the development of morphine dependence and withdrawal were investigated in the double click auditory evoked potential (EP) suppression paradigm. Rats were made dependent upon morphine hydrochloride by a series of injections (every 12h) over 6 days, followed by withdrawal after stopping morphine administration. Hippocampal gating was examined during the development of dependence and withdrawal. Moreover, the DA antagonist haloperidol was used to assess the contribution of dopamine to hippocampal gating induced by morphine. Our results showed that the morphine-treated rats exhibited significantly disrupted hippocampal gating during the development of morphine dependence and this disrupted gating was partially reversed by haloperidol pretreatment. In contrast, there was significantly enhanced hippocampal gating at the fifth and sixth days of withdrawal. The dynamics of hippocampal gating during the development of morphine dependence and withdrawal suggests the interaction between the hippocampus and opioids. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Resumo:
以稀释液TTE (382 mOsm/ kg) 为对照, 研究了5 种渗透压(688 、389 、329 、166 、43 mOsm/ kg) 的TEST 稀释液(TEST、mTEST1、mTEST2、mTEST3、mTEST4) 在冷冻过程中对猕猴精子功能的影响。精液一 步稀释于含甘油的防冻液中, 甘油的终浓度为5 % (v/ v) 。在冷冻前后分别检测精子的运动度和质膜完整性, 后者用Hoechst 33342 和碘化丙锭双色标记流式细胞术分析。结果表明: 冷冻之前, 与鲜精相比, 用TEST 和 mTEST4 稀释的精子运动度和质膜完整性显著降低( P < 01001) , 其余组中除mTEST2 稀释的精子质膜完整性显 著降低( P < 0105) 外, 精子运动度无差异; 冷冻复苏后, TTE、mTEST3 和mTEST1 冻存精子的运动度和质膜 完整性最高, 其次是mTEST2 , TEST和mTEST4 冷冻效果最差( P < 0105) 。提示等渗、适当高渗或低渗的稀释 液适合猕猴精子的冷冻保存; 对精子产生高渗毒害作用是导致猕猴精子用TEST 冷冻存活率低的主要原因。
Resumo:
Hilsa shad, Tenuolosa ilisha, belongs to Clupeidae family and Alsinae subfamily is an euryhaline pelagic and anadromous species living in marine and freshwater waters. Regarding to study the biological characteristics of this species, this study was carried out in the Northern Persian Gulf within Bushehr province waters during years 2006-7. A total of 344 specimens were collected and transported to the laboratory for further different biological measurements consist of: reproduction (GSI, fecundity, maturity stages), feeding (stomach contents, food preference ...), aging; 58 morphometric and ٧ meristic measurements. The results indicate that minimum, maximum and mean body weight are 203, 953 and 481.35±147.64 g, respectively and this values for total length are 26.5, 45.1 and 35.99±3.98 cm. For food regimes, the most abundant phytoplankton foods belong to Bacillariophyta (83.74٪) and zooplanktons of Arthropoda (51٪). The mean RLG was measured about 1.53±0.22 and the mean feeding intensity was about 51.79±38.13. Also, the aging of studied fishes showed that they have 2 to 4 years and most of the samples had 2+ years. The reproduction studies showed that Indian shad is a batch spawner species. The average gonadosomatic index (GSI) is 1.64±1.382 and the main spawning season was determined for April-May.
Resumo:
采用样方方法对栖息于云南省哀牢山平河(E 101°17′16.1″,N 24°20′09.5″,海拔2 600 m)的黑长臂猿(Nomascus concolor)栖息地乔木层结构进行了调查.调查中共记录到乔木57种隶属于23科37属;木质藤本植物9种隶属于6科8属.优势科主要为杜鹃花科(Ericaceae)、木兰科(Magnoliaceae)、山茶科(Theaceae)和壳斗科(Fagaceae)植物.主要树种重要值排序表明,露珠杜鹃(Rhododendron irroratum)为该地区最主要的优势种,在各坡位中均有广泛分布.乔木的多样性指数、均匀度指数在沟底明显降低,而乔木1层和2层所占的比例以及木质藤本的平均多度均随着坡位的下降而升高.与其他地区的长臂猿相比,哀牢山黑长臂猿的活动程度较低(10-22 m),果实性食物种类较少.
Resumo:
系统整理了我国西南及其邻近地区的社鼠标本,主成分分析(PC)和判别分析(DA)的结果也表明上述两地的标本都各自形成一个有别于指名亚种的类群,故分别将上述两地的标本订为2新亚种:雅江亚种(N. c. yajianensis subsp.nov.)和德钦亚种(N.c.deqinensis subsp.nov.)。由此确立了西南地区原社鼠指名亚种至少应分为3个亚种。
Resumo:
Biological aspects, population dynamics and stock assessment of the Caspian Sea prawns Palaemon adspersus and Palaemon eleganse were investigated in Guilan coastal water of the Caspian Sea. Sampling was done monthly with a bottom trawl with mesh size of 3 mm in cod end in 0 - 5 m and 5 - 10 m depth in areas as Astra, Shafa Roud, Anzali, Chonchanan Chamkhaleh and Chaboksar during year 2002. Results of one year sampling showed that mean total length of Palaemon adspersus (pooled data) was 39.9±6.84 mm (X±SD) and mean wiegth was 1.133±0.67 g. The mean total length of females and males was 41.6±7.5 mm and 37.9±5.2 mm respectively and mean weight for the mentioned sexes was 1.353±0.65 g and 0.868±0.38 g respectively. There was significant differences in mean length and weight of females and males (P<0.05). The mean total annual sex ratio of males: females for this species was 1.4 and this sex ratio deviated significantly from 1:1 (X2, P<0.05) and biased towards males in the population of this species. The spawning season of Palaemon adspersus begins in April and ends in September with a peak in June . Mean fecundity of this species was 1994.5 ± 506.6 . The growth coefficients Loo and K for females were estimated as 58.5 mm and 2.3 /Year and for males as 55.9 mm and 2.6 /year respectively . The mean CPUA ( catch / Km2 ) for this species was 9.99 ± 33.2 kg / km2 and the correspondance biomass was calculated as 5067.7 kg in 0 - 10 m depth . The mean total length of Palaemon elegans (pooled data ) was 27.5 ± 5.7 mm (X±S.D) and mm and 24.01±4.18 mm respectively and mean weight for the mentioned sexes were was 0.553 ± 0.3 g and 0.237±0.15 g respectively. There was significant differences in mean length and weight of females and males (P<0.05). The mean total annual sex ratio of males:females for this species was 0.57 and in this species also sex ratio differed significantly from 1:1 (X2, P<0.05) and skewed towards females in the population of this species. The spawning season of Palaemon elegans extended from May to September with a peak in July . Mean fecundity of this species was 642.7±313.4. The growth coefficients LOO and K for females were estimated as 42.119 mm and 2.40 /Year and 33.87 mm and 2.50 /year for males respectively. The mean. CPUA ( catch/ Km2 ) for this species was 0.75±3.86 kg/km2 and the correspondance biomass was calculated as 382.1 kg in 0-10 m depth .