982 resultados para 1517
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Este trabalho teve como objetivo principal a reflexão acerca do processo comunicacional das organizações empresariais frente ao novo paradigma da sustentabilidade e à necessidade de divulgação de suas iniciativas junto aos seus stakeholders. Este estudo exploratório contou com as etapas de revisão bibliográfica, pesquisa de campo junto a três instituições bancárias e análise documental de seus relatórios de sustentabilidade. Pode-se observar a transformação pela qual passa a comunicação organizacional, com a inclusão de novos públicos e a necessidade crescente de interatividade.
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Resumo: O número de diagonais de um polígono é um dos conteúdos relacionados do eixo Números e Operações dos Parâmetros Curriculares Nacionais do Ensino Fundamental, anos finais. Este relato de experiência, apesar de abordar uma atividade realizada em um Curso de Pedagogia, apresenta uma possibilidade para a contagem das diagonais de um polígono, proposta por alunos daquele curso, que pode servir como estímulo para levar um aluno, inclusive da Educação Básica, a generalizar esse conteúdo por meio de uma expressão equivalente à expressão que, geralmente, é conhecida. Ao mesmo tempo, convida o leitor a refletir sobre a prática docente,frente a uma aula em que os alunos são solicitados a construir estratégias para a resolução de atividades cujo objetivo não é a simples aplicação ou fixação de fórmulas.
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O presente estudo buscou verificar se há diferenças significativas na utilização de práticas e ferramentas voltadas à gestão do conhecimento, na opinião de trabalhadores presenciais e de teletrabalhadores. Para tanto, este estudo descritivo-quantitativo aplicou um questionário a 319 colaboradores, sendo 180 trabalhadores presenciais e 139 teletrabalhadores, todos atuantes em quinze empresas de porte médio ou grande da indústria de software no Brasil. Os resultados encontrados indicam que os teletrabalhadores pesquisados atestaram maior utilização de práticas e de ferramentas voltadas à gestão do conhecimento em suas rotinas de trabalho, em comparação aos trabalhadores presenciais analisados na pesquisa.
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A complexidade de gerir uma empresa no contexto atual, onde as mudanças e exigências dos clientes são cada vez mais dinâmicas, despertou a necessidade de uma reavaliação nos sistemas de medição. Na busca de um sistema de indicadores de desempenho que permitisse às empresas uma orientação correta dentro desta nova realidade, surgiu o Balanced Scorecard, um sistema onde as medidas financeiras são complementadas com medidas não financeiras. O Balanced Scorecard visa traduzir a estratégia da empresa em objetivos e medidas dentro de quatro perspectivas: financeira, clientes, processos internos e aprendizado e crescimento. Os métodos de elaboração do Balanced Scorecard foram desenvolvidos na sua maioria, a partir de experiências com empresas de grande porte. Apesar disto, acredita-se que esta ferramenta pode ser útil às pequenas empresas, suprindo a carência destas no que se refere a planejamento e gestão estratégica. Sendo assim, este trabalho visa propor uma sistemática de elaboração do Balanced Scorecard adaptado para o caso particular das empresas de pequeno porte. Este estudo envolve uma revisão bibliográfica, acompanhamento da aplicação da ferramenta numa empresa prestadora de serviços, e finalmente uma proposta e discussão sobre uma nova sistemática de elaboração do Balanced Scorecard para pequenas empresas. As conclusões do trabalho indicam que o método é uma ferramenta que pode auxiliar a traduzir a estratégia da empresa e pode ser aplicada às pequenas empresas, desde que sejam respeitadas suas características básicas.
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O artigo começa por discutir "cultura escolar" e a necessidade de uma mudança de paradigma educacional. Em seguida, discute a contradição entre o objectivo de educar professores inovadores na utilização de Tecnologias da Informação e Comunicação (TIC) e a tentativa de fazer isso sob a influência da cultura tradicional embutida nas paredes e nas rotinas da escola, invocando Seymour Papert, como um dos principais críticos da escola fabril. Finalmente, é avançada uma possível forma de contornar aquela contradição, desde que os formadores de professores estejam conscientes de que ela existe e abordem a formação dos professores segundo uma abordagem construtivista e de acordo com o princípio de isomorfismo, formulado por Gaston Mialaret.
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Agricultural and agro-industrial residues are often considered both an environmental and an economical problem. Therefore, a paradigm shift is needed, assuming residues as biorefinery feedstocks. In this work cherimoya (Annona cherimola Mill.) seeds, which are lipid-rich (ca. 30%) and have a significant lignocellulosic fraction, were used as an example of a residue without any current valorization. Firstly, the lipid fraction was obtained by solvent extraction. Extraction yield varied from 13% to 28%, according to the extraction method and time, and solvent purity. This oil was converted into biodiesel (by base-catalyzed transesterification), yielding 76 g FAME/100 g oil. The obtained biodiesel is likely to be incorporated in the commercial chain, according to the EN14214 standard. The remaining lignocellulosic fraction was subjected to two alternative fractionation processes for the selective recovery of hemicellulose, aiming different products. Empirical mathematical models were developed for both processes, aiming future scale-up. Autohydrolysis rendered essentially oligosaccharides (10 gL-1) with properties indicating potential food/feed/pharmacological applications. The remaining solid was enzymatically saccharified, reaching a saccharification yield of 83%. The hydrolyzate obtained by dilute acid hydrolysis contained mostly monosaccharides, mainly xylose (26 gL-1), glucose (10 gL-1) and arabinose (3 gL-1), and had low content of microbial growth inhibitors. This hydrolyzate has proven to be appropriate to be used as culture media for exopolisaccharide production, using bacteria or microbial consortia. The maximum conversion of monosaccharides into xanthan gum was 0.87 g/g and kefiran maximum productivity was 0.07 g.(Lh)-1. This work shows the technical feasibility of using cherimoya seeds, and materials as such, as potential feedstocks, opening new perspectives for upgrading them in the biorefinery framework.
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A large number of evidences correlate elevated levels of homocysteine (Hcys) with a higher cardiovascular diseases (CVDs) risk, especially, atherosclerosis. Similarly, abnormal low levels of the vitamins B6, B9 and B12 are associated to an instability in the methionine cycle with an over production of Hcys. Thus, biomedical sciences are looking forward for a cheaper, faster, precise and accurate analytical methodology to quantify these compounds in a suitable format for the clinical environment. Therefore the objective of this study was the development of a simple, inexpensive and appropriate methodology to use at the clinical level. To achieve this goal, a procedure integrating a digitally controlled (eVol®) microextraction by packed sorbent (MEPS) and an ultra performance liquid chromatography (UPLC) coupled to a photodiode array detector (PDA) was developed to identify and quantify Hcys vitamins B6, B9 and B12. Although different conditions were assayed, we were not able to combine Hcys with the vitamins in the same analytical procedure, and so we proceeded to the optimization of two methods differing only in the composition of the gradient of the mobile phase and the injected volume. It was found that MEPS did not bring any benefit to the quantification of the Hcys in the plasma. Therefore, we developed and validate an alternative method that uses the direct injection of treated plasma (reduced and precipitated). This same method was evaluated in terms of selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), matrix effect and precision (intra-and inter-day) and applied to the determination of Hcys in a group composed by patients presenting augmented CVD risk. Good results in terms of selectivity and linearity (R2> 0.9968) were obtained, being the values of LOD and LOQ 0.007 and 0.21 mol / L, respectively. The intra-day precision (1.23-3.32%), inter-day precision (5.43-6.99%) and the recovery rate (82.5 to 93.1%) of this method were satisfactory. The matrix effect (>120%) was, however, higher than we were waiting for. Using this methodology it was possible to determine the amount of Hcys in real plasma samples from individuals presenting augmented CVD risk. Regarding the methodology developed for vitamins, despite the optimization of the extraction technique and the chromatographic conditions, it was found that the levels usually present in plasma are far below the sensitivity we obtained. Therefore, further optimizations of the methodology developed are needed. As conclusion, part of the objectives of this study was achieved with the development of a quick, simple and cheaper method for the quantification of Hcys.
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FEHR, Guilherme Lotierso et al. Efetividade dos exercícios em cadeia cinética aberta e cadeia cinética fechada no tratamento da síndrome da dor femoropatelar. Revista Brasileira de Medicina do Esporte, [s.l], v. 12, n. 2, p.66-70, mar./abr. 2006. Bimestral. Disponível em:
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DAVIM, Rejane Marie Barbosa;ENDERS, Bertha Cruz; DANTAS, Janmilli da Costa; SILVA, Richardson Augusto Rosendo da; NÓBREGA, Edualeide Jeane Pereira Bulhões da. Método mãe-canguru: vivência de mães no alojamento conjunto. Revista da Rede de Enfermagem do Nordeste, Fortaleza, v. 10, n. 1, p. 37-44, jan./mar.2009.
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Analisar a associação estatística entre diagnósticos de enfermagem identifi cados nos pacientes transplantados renais e seus fatores sócio-econômicos. Estudo transversal realizado em um hospital universitário em Fortaleza-CE, no período compreendido entre dezembro de 2004 a abril de 2005. Foram identifi cados 38 diagnósticos de enfermagem. Dez diagnósticos apresentaram freqüências acima do percentil 75: Risco de infecção; percepção sensorial perturbada: visual; Padrão de sono perturbado; Nutrição desequilibrada: mais do que as necessidades corporais; Fadiga; Disfunção sexual; Percepção sensorial perturbada: audição; Dor aguda; Padrões de sexualidade inefi cazes; Risco de nutrição desequilibrada: mais do que as necessidades. O estudo sobre diagnósticos de enfermagem ajudou a expandir o conhecimento da realidade dos pacientes, o que é necessário para o estabelecimento de cuidados de enfermagem ao transplante renal, bem como para abordar os resultados que vierem a ser desenvolvidas com o objetivo de melhorar a qualidade de vida
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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In the present article we report on the biological characterization and amino acid sequence of a new basic Phospholipases A(2) (PLA(2)) isolated from the Crotalus durissus collilineatus venom (Cdcolli F6), which showed the presence of 122 amino acid residues with a pI value of 8.3, molecular mass of 14 kDa and revealed an amino acid sequence identity of 80% with crotalic PLA(2)s such as Mojave B, Cdt F15, and CROATOX. This homology, however, dropped to 50% if compared to other sources of PLA(2)s such as from the Bothrops snake venom. Also, this PLA(2) induced myonecrosis, although this effect was lower than that of BthTx-I or whole crotoxin and it was able to induce a strong blockage effect on the chick biventer neuromuscular preparation, independently of the presence of the acid subunid (crotapotin). The neurotoxic effect was strongly reduced by pre-incubation with heparin or with anhydrous acetic acid and rho-BPB showed a similar reduction. The rho-BPB did not reduce significantly the myotoxic activity induced by the PLA(2), but the anhydrous acetic acid treatment and the pre-incu-bation of PLA(2) with heparin reduced significantly its effects. This protein showed a strong antimicrobial activity against Xanthomonas axonopodis passiflorae (Gram-negative), which was drastically reduced by incubation of this PLA(2) with rho-BPB, but this effect was marginally reduced after treatment with anhydrous acetic acid. Our findings here allow to speculate that basic amino acid residues on the C-terminal and molecular regions near catalytic site regions such as Calcium binding loop or rho-wing region may be involved in the binding of this PLA(2) to the molecular receptor to induce the neurotoxic effect. The bactericidal effect, however, was completely dependent on the enzymatic activity of this protein.
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Columnaris disease is one of the main causes of mortality in tilapia rearing and is responsible for large economic losses worldwide. Hematology is a tool that makes it possible to study organisms' physiological responses to pathogens. It may assist in making diagnoses and prognoses on diseases in fish populations. The hematological variables of nile tilapia were studied in specimens with a clinical diagnosis of columnaris disease and in specimens that were disease-free. The total erythrocyte count, hemoglobin rate, hematocrit percentage, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), organic defense blood cell percentages (leukocytes and thrombocytes) and hepatosomatic and splenosomatic index were determined. The results showed that there were changes in the erythrocytic series and in organic defense blood cells, in the fish infected with the bacterium, with reductions in erythrocytic variables and significant increases in the numbers of circulating lymphocytes and neutrophils.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Biosurfactants are bioactive agents that can be produced by many different microorganisms. Among those, special attention is given to yeasts, since they can produce many types of biosurfactants in large scale, using several kinds of substrates, justifying its use for industrial production of those products. For this production to be economically viable, the use of residual carbon sources is recommended. The present study isolated yeasts from soil contaminated with petroleum oil hydrocarbons and assessed their capacity for producing biosurfactants in low cost substrates. From a microbial consortium enriched, seven yeasts were isolated, all showing potential for producing biosurfactants in soybean oil. The isolate LBPF 3, characterized as Candida antarctica, obtained the highest levels of production - with a final production of 13.86 g/L. The isolate LBPF 9, using glycerol carbon source, obtained the highest reduction in surface tension in the growth medium: approximately 43% of reduction after 24 hours of incubation. The products obtained by the isolates presented surfactant activity, which reduced water surface tension to values that varied from 34 mN/m, obtained from the product of isolates LBPF 3 and 16 LBPF 7 (respectively characterized as Candida antarctica and Candida albicans) to 43 mN/m from the isolate LPPF 9, using glycerol as substrate. The assessed isolates all showed potential for the production of biosurfactants in conventional sources of carbon as well as in agroindustrial residue, especially in glycerol.