979 resultados para 13368-055
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Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB
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ABSTRACT: Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational Taxonomic Unit (OTU) forming a clade with the Methanosarcinales according to low bootstrap values. Homology modeling was used to determine the three-dimensional (3D) structures, for this the partial nucleotide sequence of the mcrα were isolated and translated on their partial amino acid sequences. The 3D structures of the archaean mcrα observed in the present study varied little, and presented approximately 70% identity in comparison with the mcrα of Methanopyrus klanderi. The results demonstrated that the community of methanogenic archaeans of the anoxic C1 region of the Tucurui reservoir is relatively homogeneous.
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Pós-graduação em Zootecnia - FMVZ
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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In Brazil, common bean is in one of the most representative economic crops, not only because of its growing area but also because of the economic value of its production. In the crop year 2006/07, in Selvíria (MS), we analyzed the common bean yield as a function of some physical attributes of a Typic Acrustox in minimum tillage and center pivot irrigation system. The aim of this work was to evaluate among the soil physical attributes, under minimum tillage, those that better explain the variability of bean yield using the Pearson and spatial correlations,. A geostatistical grid was installed to collect data from soil and plants, with 117 sampling points in an area of 2025 m² and homogeneous slope of 0.055 m m-1. The results showed a low yield of bean, which occurred probably due to a lower density of plants that the system provided. Thus, for the minimum tillage system, the bean yield could be explained as a function of total porosity and bulk density.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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In crop year 2006/07, in Selviria, MS, Brazil, were analyzed the productivity of beans because of the chemical attributes of an Acrustox cultivated under conditions of high technological level of management by no-tillage irrigated with pivot central. The objective was to select, among the attributes studied soil, the one with the best representation to explain the variability of agricultural productivity. Geostatistical grid was installed to collect data from soil and plant, with 117 sampling points in an area of 2,025 m(2) and homogeneous slope of 0.055 m m(-1). From the standpoint of linear and spatial bean yield was respectively explained in terms of P and soil pH. So much for the values of phosphorus (P) in the intermediate layer and subsurface between 24-26 mg dm(-3), as well as for Hydrogen (pH) in the surface layer between 5.0 to 5.4, resulted in sites with the most high yield (2,160-2,665 kg ha(-1)).
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The objective of this study was to evaluate the behaviour of fibre in the digestive tract on the basis of the passage kinetics of forage and concentrate particles in cows fed different omega-6 fatty-acid sources. The scientific hypothesis of this study was that omega-6 fatty acids do not interfere with the digestion of fibre in the diets of dairy cows. Five primiparous lactating Holstein cows were used in the experiment. The experimental diets were: control (C), ground soyabean (GS), cottonseed (CS), soyabean oil (SO), calcium salts of fatty acids (CSFA). The global mean estimates for the parameters of passage rate (gamma) were 0.038 and 0.055 h(-1) for forage and concentrate, respectively. The only significant effect with respect to the passage rate was a high negative correlation between the concentrate passage rate and dry matter intake. There was less undegradable neutral detergent fibre (NDF) in treatments without added lipid. Dietary supplementation with lipid sources does not alter the kinetic parameters of roughage and concentrate particle passage or in vitro NDF degradation. Sources of omega-6 fatty acids do not alter the rumen degradation and transit of fibre.
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This study aimed to evaluate season, breed, number of lactations and milk production as risk factors relating to Rhipicephalus microplus infestation in dairy cows during the peripartum. Eighty-four animals were randomly selected through proportional stratified sampling. All engorged and partially engorged female R. microplus specimens measuring 4.5-8.0 mm were counted during the 5 weeks before calving, calving week and 5 weeks after calving. The peripartum had a significant effect [calving (p < 0.05; prevalence ratio (PR)= 3.12) and post calving (p < 0.05; PR = 2.02)] on R. microplus infestation. Although the average tick count was higher during the rainy season than during the dry season, there was no significant difference (p = 0.055; PR = 0.63). The average R. microplus count was significantly higher (p < 0.05; PR = 2.10) in Bos taurus animals, followed by F1 (p < 0.05; PR = 1.64) and Girolando (p < 0.05; PR = 1.39). The average R. microplus count was significantly higher (p < 0.05; PR = 0.97) in first-lactation animals, followed by those at the second, third and, fourth or subsequent lactation. Milk production showed a negative correlation with R. microplus count, such that high-production animals were significantly (p = 0.003; PR = 2.04) more vulnerable to infestation than were low-production animals. First-lactation and high-production B. taurus animals had greatest vulnerability to R. microplus infestation over the peripartum period, and constituted the at-risk group in the dairy herd.u
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Zootecnia - FCAV
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)