Overexpression of csk inhibits acid-induced activation of NHE-3.

Opossum kidney OKP cells express an apical membrane Na+/H+ antiporter that is encoded by NHE-3 (for Na+/H+ exchanger 3) and is similar in many respects to the renal proximal tubule apical membrane Na+/H+ antiporter. Chronic incubation of OKP cells in acid medium for 24 hr increases Na+/H(+)-antiporter activity and NHE-3 mRNA abundance. The increase in Na+/H(+)-antiporter activity was not prevented by H7, a protein kinase C/protein kinase A inhibitor, but was prevented by herbimycin A, a tyrosine kinase inhibitor. Incubation of cells in acid medium increased c-src activity, and this was inhibited by herbimycin A. To determine the role of the src family of nonreceptor protein-tyrosine kinases, Csk (for carboxyl-terminal src kinase), a physiologic inhibitor of these kinases, was overexpressed in OKP cells. In three clones overexpressing csk, acid-induced increases in Na+/H(+)-antiporter activity and NHE-3 mRNA abundance were inhibited. In these clones, inhibition of acid activation of Na+/H(+)-antiporter activity paralleled inhibition of acid activation of c-src. Neither herbimycin A nor overexpression of csk inhibited dexamethasone-induced increases in Na+/H(+)-antiporter activity. These studies show that decreases in pH activate c-src and that the src family nonreceptor protein-tyrosine kinases play a key role in acid activation of NHE-3.

Textile fabrics of ancient Peru

by William H. Holmes.

Textile fabrics of ancient Peru

by William H. Holmes.

Interleukin-6-deficient mice are highly susceptible to Giardia lamblia infection but exhibit normal intestinal immunoglobulin A responses against the parasite.

In the present study, interleukin-6 (IL-6)-deficient mice were infected with Giardia lamblia clone GS/M-83-H7. Murine IL-6 deficiency did not affect the synthesis of parasite-specific intestinal immunoglobulin A. However, in contrast to wild-type mice, IL-6-deficient animals were not able to control the acute phase of parasite infection. Reverse transcription-PCR-based quantitation of cytokine mRNA levels in peripheral lymph node cells exhibited a short-term up-regulation of IL-4 expression in IL-6-deficient mice that seemed to be associated with failure in controlling the parasite population. This observation suggests a further elucidation of IL-4-dependent, Th2-type regulatory processes regarding their potential to influence the course of G. lamblia infection in the experimental murine host.

Abyssal community analysis from replicate box cores in the central North Pacific

A 0.25 m**2 United States Naval Electronics Laboratory box corer was used to take replicate samples from an oligotrophic bottom under the North Pacific Central Water Mass (~28°N, 155°W). The bottom is a red clay with manganese nodules at a depth of 5500-5800 m. Macrofaunal density ranges from 84 to 160 individuals per m**2 and is therefore much the same as in Northwest Atlantic Gyre waters. Of the macrofaunal taxa, polychaetes dominate (55 %), followed by tanaids (18 %), bivalves (7 %), and isopods (6 %). Meiofaunal taxa were only partially retained by the 297 µm screen used in washing. Even then, they are 1.5-3.9 times as abundant as the macrofaunal taxa, with nematodes being numerically dominant by far. Foraminifera seem to comprise an important portion of the community, but could not be assessed accurately because of the inability to discriminate living and dead tests. Remains of what are probably xenophyophoridans are also very important, but offer the same problem. Faunal diversity is extremely high, with deposit feeders comprising the overwhelming majority. Most species are rare, being encountered only once. The distributions of only three species show any significant deviation from randomness. The polychaete fauna from box cores collected from 90 miles to the north was not significantly different from that of the principal study locality. Concordance appeared at several taxonomic levels, from species through macrofaunal/meiofaunal relationships. As a result, the variation in total animal abundance shows aggregation among cores. We discuss Sokolova's concept of a deep-sea oligotrophic zone dominated by suspension feeders, and reconcile it with our present findings. The high diversity of the fauna combined with the low food level contradict theories that relate diversity directly with productivity.

Description courte et abrégée des salines du gouvernement d'Aigle /

Mode of access: Internet.

La logica o El órden natural del raciocinio ... /

Mode of access: Internet.

Venerabilis patris Nicolai Lancicii è Societate Jesu Opera omnia spiritualia XXI opusculis comprehensa : in quibus virtutes cuilibet hominum statui conuenientes tum solida doctrina illustrantur, tum piis affectibus exornantur.

Mode of access: Internet.

Oeuvres complettes d'Ovide : traduites en français : auxquelle on a ajouté la vie de ce poëte ; les Hymnes de Callimaque ; l'Epitre de Lingedes sur l'exil d'Ovide ; et la traduction en vers de la belle Elégie d'Ovide sur son départ /

Mode of access: Internet.

Examen de las indulgencias que legitimamente gozan las religiones, y cofradias, segun las mas modernas decisiones y decretos de los Romanos Pontifices ... /

Mode of access: Internet.

Relaçam feita em consistorio secreto diante do ... Vrbano Papa Octauo /

Mode of access: Internet.

Jacobi Altingi... Fundamenta punctationis linguae sanctae : cum necessariis Canonum, Locorum S. Scripturae et Vocum Irregularium Indicibus : Accedit ejusdem Synopsis Institutionum Chaldaearum et Syrarum Editio Nona : Simili Institutionum Samaritanarum Rabbinicarum, Arabicarum, Aethiopicarum et Persicarum Synopsi /

Contiene con portada y paginación propia : Jacobi Altingi Synopsis institutionum Chaldaeorum et Syrarum, [4], 122 p., con port. propia y año 1747 y Georgii Othonis, ... Synopsis institutionum samaritanarum... [16], 176 p., con portada propia y año 1735.

The snake-dance of the Moquis of Arizona; being a narrative of a journey from Santa Fé, New Mexico, to the villages of the Moqui Indians of Arizona, with a description of the manners and customs of this peculiar people, and especially of the revolting religious rite, the snake-dance; to which is added a brief dissertation upon serpent-worship in general, with and account of the tablet dance of the pueblo of Santo Domingo, New Mexico, etc.

Mode of access: Internet.

Apophthegmata ex probatis graecae latinaeque linguae scriptoribus /

Sign.: œ8, A-Z8, 2A-2Z8, 3A-3B8 ; *8, a-g8, h7 +

Thiamine Deficiency Results in Downregulation of the GLAST Glutamate Transporter in Cultured Astrocytes

Pyrithiamine-induced thiamine deficiency (TD) is a well-established model of Wernicke's encephalopathy in which a glutamate-mediated excitotoxic mechanism may play an important role in determining selective vulnerability. In order to examine this possibility, cultured astrocytes were exposed to TD and effects on glutamate transport and metabolic function were studied. TD led to decreases in cellular levels of thiamine and thiamine diphosphate (TDP) after 24 h of treatment and decreased activities of the TDP-dependent enzymes alpha-ketoglutarate dehydrogenase and transketolase after 4 and 7 days, respectively. TD treatment for 10 days led to a reversible decrease in the uptake of [H-3]-D-aspartate, a nonmetabolizable analogue of glutamate. Kinetic analysis revealed that the uptake inhibition was caused by a 47% decrease in the V-max for uptake of [H-3]-D-aspartate, with no change in the K-m value. Immunoblotting showed that this decrease in uptake was due to an 81% downregulation of the astrocyte-specific GLAST glutamate transporter. Loss of uptake activity and GLAST protein were blocked by treatment with the protein kinase C inhibitor H7, while exposure to DCG IV, a group II metabotropic glutamate receptor (mGluR) agonist, resulted in improvement of [H-3]-D-aspartate uptake and a partial reversal of transporter downregulation. These results are consistent with our recent in vivo findings of a loss of astrocytic glutamate transporters in TD and provide evidence that TD conditions may increase phosphorylation. of GLAST, contributing to its downregulation. In addition, manipulation of group II mGluR activity may provide an important strategy in the treatment of this disorder. (C) 2003 Wiley-Liss, Inc.