897 resultados para preference for routine


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O presente trabalho teve como objetivo principal analisar as mudanças nas formas de venda e consumo do açaí na Região Metropolitana de Belém (RMB), e sua relação com a identidade de lugar de seus consumidores. O interesse pelo tema teve origem na observação da ocorrência da globalização do açaí, alimento base da dieta das populações amazônicas de origem ribeirinha que hoje é largamente consumido fora da Região Amazônica, em padrões muito diferentes daqueles nela estabelecidos. A escolha da RMB como lócus de pesquisa se justifica pelo fato de que nela se concentra alto consumo de açaí, nos padrões tradicionais e nos modernos, suscitando questionamentos sobre os efeitos dessa convivência na vida cotidiana dos que a habitam. Teoricamente, a pesquisa articula a definição de identidade de lugar de Abe-Lima (2012) com as elaborações de Henri Lefèbvre (1991) sobre a vida cotidiana no mundo moderno e com teoria dos circuitos econômicos de Milton Santos (2008). Metodologicamente, foi adotada uma estratégia multimétodos, envolvendo pesquisa documental, um painel de especialistas com 5 entidades de acompanhamento da produção de açaí, observações pautadas na etnografia urbana em 9 locais de venda e entrevistas semiabertas com 40 consumidores de açaí, maiores de 18 anos e residentes na RMB, recrutados por meio de cadeia tipo bola de neve. Além dos roteiros de entrevistas, foram utilizados como estratégias de coleta de dados diários de campo e fotografias. O conjunto dos dados foi submetido à técnica da análise de conteúdo temática, com eventual apoio do software QDA Miner Lite. Os resultados situaram a globalização do açaí em meados da década de 1990, momento de intensificação dos efeitos da metropolização em Belém, como o aumento populacional, a segregação espacial e a imposição de um ritmo de vida mais acelerado. As análises apontaram que tal processo significou a entrada do açaí no circuito superior da economia, com um processo produtivo mais organizado, utilizando mais tecnologia e envolvendo maiores volumes financeiros, atingindo escala industrial. No entanto, prevalece na RMB o processamento artesanal, em estruturas de pequeno porte, construídas em alvenaria, de organização simples e controle financeiro ausente. O consumo público aumentou, com grande número de locais especializados na oferta de açaí, em caráter de entretenimento, sendo incipiente a adesão a novos acompanhamentos, como granola e aveia. A preferência pelos acompanhamentos tradicionais, como farinha d’água ou de tapioca, açúcar e peixe, permanece, assim como pelo consumo privado, no âmbito da casa, que tem sido favorecido pela disponibilidade do serviço de entrega, recurso recente. A frequência geral de consumo diminuiu, em função do aumento de preço e de mudanças na rotina, como aumento da distância entre local de trabalho e residência. A pesquisa permitiu reafirmar a importância do açaí na vida cotidiana dos belenenses que o consomem, para os quais ele funciona como elemento de distintividade, continuidade, autoestima e autoeficácia, em estreita relação com aspectos próprios da metrópole. Por esse motivo, torna-se preocupante que o acesso a açaí de qualidade, nos padrões desejados pelos indivíduos, esteja tornando-SE privilégio inacessível aos consumidores de menor poder aquisitivo. Assim, espera-se que o conjunto de dados contribua para a compreensão da importância de políticas públicas voltadas ao açaí que considerem melhor as demandas daqueles que o consomem.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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BackgroundConditioned place preference (CPP) to ethanol (EtOH) is an important addiction-related alteration thought to be mediated by changed neurotransmission in the mesocorticolimbic brain pathway. Stress is a factor of major importance for the initiation, maintenance, and reinstatement of drug abuse and modulates the neurochemical outcomes of drugs. Thus, the aim of this study was to investigate the effects of concomitant exposure to chronic EtOH and stress on CPP to this drug and alterations of dopaminergic and serotonergic neurotransmission in mice.MethodsMale Swiss mice were chronically treated with EtOH via a liquid diet and were exposed to forced swimming stress. After treatment, animals were evaluated for conditioning, extinction, and reinstatement of CPP to EtOH. Also, mice exposed to the same treatment protocol had their prefrontal cortex (PFC), nucleus accumbens (NAc), and amygdala dissected for the quantitation of dopamine, serotonin, and their metabolites content.ResultsData showed that previous chronic exposure to EtOH potentiated EtOH conditioning and increased dopaminergic turnover in PFC. Exposure to stress potentiated EtOH conditioning and decreased dopaminergic turnover in the NAc. However, animals exposed to both chronic EtOH and stress did not display alterations of CPP and showed an elevated content of dopamine in amygdala. No treatment yielded serotonergic changes.ConclusionsThe present study indicates that previous EtOH consumption as well as stress exposure induces increased EtOH conditioning, which can be related to dopaminergic alterations in the PFC or NAc. Interestingly, concomitant exposure to both stimuli abolished each other's effect on conditioning and PFC or NAc alterations. This protective outcome can be related to the dopaminergic increase in the amygdala.

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Objective. The myeloid-related proteins 8 and 14 (MRP-8/MRP-14) and neutrophil-derived S100A12 are biomarkers of inflammation. They can be used to determine the relapse risk in patients with juvenile idiopathic arthritis (JIA) after stopping antiinflammatory treatment. In this study, we tested the performance of different enzyme-linked immunosorbent assays (ELISAs) in order to validate systems available for routine use.Methods. MRP-8/MRP-14 and S100A12 serum concentrations of 188 JIA patients in remission were analyzed. Commercially available test systems were compared to experimental ELISAs established in house. The ability of the assays to identify JIA patients at risk for relapse was analyzed.Results. For MRP-8/MRP-14, the PhiCal Calprotectin and Buhlmann MRP8/14 Calprotectin ELISAs revealed hazard ratios of 2.3 and 2.1, respectively. For S100A12, the CircuLex S100A12/EN-RAGE ELISA revealed a hazard ratio of 3.1. The commercial assays allowed a JIA relapse prediction that was at least comparable to the experimental ELISAs.Conclusion. For the prediction of JIA relapse after stopping medication, the biomarkers MRP-8/MRP-14 and S100A12 can be determined by using assays that are available for routine use. The tested commercial MRP-8/MRP-14 and S100A12 ELISAs showed a performance comparable to well-established experimental ELISA protocols when assay-specific cutoffs for the indication of relapse prediction are thoroughly applied.

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Glomerulitis and peritubular capillaritis have been recognized as important lesions in acute renal rejection (AR). We studied glomerulitis and peritubular capillaritis in AR by 2 methods and investigated associations with C4d, type/grade of AR, and allograft survival time. Glomerulitis was measured according to Banff scores (glomerulitis by Banff Method [gBM]) and by counting the number of intraglomerular inflammatory cells (glomerulitis by Quantitative Method [gQM]). Capillaritis was classified by the Banff scoring system (peritubular capillaritis by Banff Method [ptcBM]) and by counting the number of cells in peritubular capillaries in 10 high-power fields (hpf; peritubular capillaritis by Quantitative Method [ptcQM]). These quantitative analyses were performed in an attempt to improve our understanding of the role played by glomerulitis and capillaritis in AR. The g0 + g1 group (gBM) associated with negative C4d (P = .02). In peritubular capillaritis, a larger number of cells per 10 hpf in peritubular capillaries (ptcQM) were observed in positive C4d cases (P = .03). The group g2 + g3 (gBM) correlated with graft loss (P = .01). Peritubular capillaritis was not significantly related to graft survival time. Our study showed that the Banff scoring system is the best method to study glomerulitis and observed that the evaluation of capillaritis in routine biopsies is difficult and additional studies are required for a better understanding of its meaning in AR biopsy specimens of renal allografts.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Some changes in the application of the numeric trapezoidal integration are analyzed for applications considering pi circuits. It is considered numeric and computational proceedings for improving the numeric results obtained with associations of pi circuits. In numeric integration solutions of the linear systems, it is common to represent these associations of pi circuits by only one matrix. This representation introduces undesirable numeric oscillations in simulations of the dynamics of wave propagation in electrical systems. The proposed changes improve the results of application of cascades of pi circuits associated to the trapezoidal integration, avoiding that the numerical oscillations, or Gibb's oscillations, have high values and are slowly damped. For the carried out simulations, different number of pi circuits and voltage sources are checked, confirming the reduction of the influence of the numeric oscillations on the obtained results. (C) 2014 Elsevier B.V. All rights reserved.

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This study aimed to verify the preference and infestation level of Anastrepha fraterculus (Diptera: Tephritidae) [South American fruit fly] in fruits of guava cultivars and to correlate them to variables such as peel coloration, soluble solids and pH of fruit cultivars. The following cultivars were used: Pedro Sato, Paluma, Casco and S,culo XXI. The infestation was evaluated in cages, considering two scenarios: no-choice and multiple choice. In both tests, evaluations of the fruit attraction to insects were conducted for a period of 1', 3', 5', 10', 20', 30', 1 h, 2 h, 6 h, 12 h and 24 h. The visit of A. fraterculus on the assayed cultivars in relation to the time was studied by logistic regression. After 10 days, the number of larvae in each fruit was recorded. In the multiple choice test, the visit proportions were significantly higher in the fruits of cvs. S,culo XXI and Pedro Sato than in those of cvs. Paluma and Casco. In the no-choice test, the visit proportions were significantly lower in the Paluma fruits. In both tests, the rate of fruit infestation by A. fraterculus did not differ among cvs. Pedro Sato, Paluma and Casco, whereas the fruits of cv. S,culo XXI were more infested. The indexes of pH did not interfere with the infestation of A. fraterculus, whereas a high rate of soluble solids and low color angle appear to be crucial for discriminating the fruits of the most susceptible cultivars. Infestation rate of S,culo XXI fruits displayed significant correlations with: A degrees Brix (r= 0.7078) and color angle (h) (r= -0.9499) of guava fruits under the multiple choice conditions.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Forest plantations with Eucalyptus spp. (L'H,r) in Brazil are highly yielding. However, this activity is more and more threatened due to interactions with phytophagous insects, especially by exotic species, such as the red gum lerp psyllid Glycaspis brimblecombei Moore (Hemiptera: Psyllidae). This study aimed to evaluate the resistance in eucalyptus against the psyllid in oviposition and biological development assays, attempting to identify potential genotypes resistant to the pest for forest plantations. In addition, we tested the hypothesis of that concentration of total phenolics and lignin, and amount of epicuticular wax were associated with the expression of resistance. Results showed that there was variation in the levels of resistance among the genotypes assessed. Oviposition non-preference was observed on Eucalyptus citriodora (Hook) in free-choice and no-choice tests, and Clone FP10 was least preferred in the no-choice test. The genotypes E. citriodora and Clone FP6 provided 100 % nymphal mortality, and Clones FP7 and FP9 also affected negatively the G. brimblecombei development by lengthening the duration of the nymphal stage and reducing adult emergence. Clone FP6 had higher concentration of total phenolics and larger contact angle formed between the water droplet and leaf surface, which may be associated with thicker layer of epicuticular wax on the leaves, and one of the causes of high nymphal mortality. Thus, the use of the resistant genotypes of eucalyptus screened against G. brimblecombei is a promising and viable alternative for forest plantations infested with this pest.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)