Protocols for conducting dolphin capture-release health assessment studies

Marine mammals, such as dolphins, can serve as key indicator species in coastal areas by reflecting the effects of natural and anthropogenic stressors. As such they are often considered sentinels of environmental and ecosystem health (Bossart 2006; Wells et al. 2004; Fair and Becker 2000). The bottlenose dolphin is an apex predator and a key component of many estuarine environments in the southeastern United States (Woodward-Clyde Consultants 1994; SCDNR 2005). Health assessments of dolphins are especially critical in areas where populations are depleted, show signs of epidemic disease and/or high mortality and/or where habitat is being altered or impacted by human activities. Recent assessments of environmental conditions in the Indian River Lagoon, Florida (IRL) and the estuarine waters surrounding Charleston, South Carolina (CHS) highlight the need for studies of the health of local bottlenose dolphins. While the condition of southeastern estuaries was rated as fair in the National Coastal Condition Report (U.S. EPA 2001), it was noted that the IRL was characterized by poorer than expected benthic communities, significant sediment toxicity and increased nutrient concentrations. Similarly, portions of the CHS estuary have sediment concentrations of aliphatic aromatic hydrocarbons, select inorganic metals, and some persistent pesticides far in excess of reported bioeffect levels (Hyland et al. 1998). Long-term trends in water quality monitoring and recent scientific research suggest that waste load assimilation, non-point source runoff impacts, contaminated sediments, and toxic pollutants are key issues in the CHS estuary system. Several ‘hot spots’ with high levels of heavy metals and organic compounds have been identified (Van Dolah et al. 2004). High concentrations of anthropogenic trace metals, polychlorinated biphenyls (PCB’s) and pesticides have been found in the sediments of Charleston Harbor, as well as the Ashley and Cooper Rivers (Long et al. 1998). Two superfund sites are located within the CHS estuary and the key contaminants of concern associated with these sites are: polycyclic aromatic hydrocarbons (PAH), lead, chromium, copper, arsenic, zinc and dioxin. Concerns related to the overall health of IRL dolphins and dermatologic disease observed in many dolphins in the area (Bossart et al. 2003) initiated an investigation of potential factors which may have impacted dolphin health. From May-August 2001, 35 bottlenose dolphins died in the IRL during an unusual mortality event (MMC 2003). Many of these dolphins were diagnosed with a variety of skin lesions including proliferative ulcerative dermatitis due to protozoa and fungi, dolphin pox and a vesicular dermatopathy of unknown etiology (Bossart et al. 2003). Multiple species from fish to dolphins in the IRL system have exhibited skin lesions of various known and unknown etiologies (Kane et al. 2000; Bossart et al. 2003; Reif et al. 2006). On-going photo-identification (photo-ID) studies have documented skin diseases in IRL dolphins (Mazzoil et al. 2005). In addition, up to 70% of green sea turtles in the IRL exhibit fibropapillomas, with the highest rates of occurrence being seen in turtles from the southern IRL (Hirama 2001).

An assessment of chemical contaminants, toxicity and benthic infauna in sediments from the St. Thomas East End Reserves (STEER)

This report contains a chemical and biological characterization of sediments from the St. Thomas East End Reserves (STEER) in St. Thomas, U.S. Virgin Islands (USVI). The STEER Management Plan (published in 2011) identified chemical contaminants and habitat loss as high or very high threats and called for a characterization of chemical contaminants as well as an assessment of their effects on natural resources. The baseline information contained in this report on chemical contaminants, toxicity and benthic infaunal community composition can be used to assess current conditions, as well as the efficacy of future restoration activities. In this phase of the project, 185 chemical contaminants, including a number of organic (e.g., hydrocarbons and pesticides) and inorganic (e.g., metals) compounds, were analyzed from 24 sites in the STEER. Sediments were also analyzed using a series of toxicity bioassays, including amphipod mortality, sea urchin fertilization impairment, and the cytochrome P450 Human Reporter Gene System (HRGS), along with a characterization of the benthic infaunal community. Higher levels of chemical contaminants were found in Mangrove Lagoon and Benner Bay in the western portion of the study area than in the eastern area. The concentrations of polychlorinated biphenyls (PCBs), DDT (dichlorodiphenyltrichloroethane), chlordane, zinc, copper, lead and mercury were above a NOAA sediment quality guideline at one or more sites, indicating impacts may be present in more sensitive species or life stages in the benthic environment. Copper at one site in Benner Bay, however, was above a NOAA guideline indicating that effects on benthic organisms were likely. The antifoulant boat hull ingredient tributyltin, or TBT, was found at the third highest concentration in the history of NOAA’s National Status and Trends (NS&T) Program, which monitors the Nation’s coastal and estuarine waters for chemical contaminants and bioeffects. Unfortunately, there do not appear to be any established sediment quality guidelines for TBT. Results of the bioassays indicated significant sediment toxicity in Mangrove Lagoon and Benner Bay using multiple tests. The benthic infaunal communities in Mangrove Lagoon and Benner Bay appeared severely diminished.

Majuro Atoll, Republic of the Marshall Islands coral reef ecosystems mapping report

Digital maps of the coral reef ecosystem (<~30m deep) of Majuro Atoll, Republic of the Marshall Islands, were created through visual interpretation of remote sensing imagery. Digital Globe’s Quickbird II satellite images were acquired between 2004 and 2006 and georeferenced to within 1.6 m of their true positions. Reef ecosystem features were digitized directly into a GIS at a display scale of 1:4000 using a minimum feature size of 1000 square meters. Benthic features were categorized according to a classification scheme with attributes including zone (location, such as lagoon or forereef, etc.), structure (bottom type, such as sand or patch reef, etc.) and percent hard bottom. Ground validation of habitat features was conducted at 311 sites in 2009. Resulting maps consisted of 1829 features covering 366 square kilometers. Results demonstrate that reef zones occurred in a typical progression of narrow bands from offshore, though forereef, reef flat, shoreline, land, backreef, and lagoon habitats. Lagoon was the largest zone mapped and covered nearly 80% of the atoll, although much of it was too deep to have structures identified from the satellite imagery. Dominant habitat structures by area were pavement and aggregate reef, which covered 29% and 18% of the mapped structures, respectively. Based on the number of features, individual and aggregated patch reefs comprised over 40% of the features mapped. Products include GIS based maps, field videos and pictures, satellite imagery, PDF atlas, and this summary report. Maps and associated data can be used to support science and management activities on Majuro reef ecosystems including inventory, monitoring, conservation, and sustainable development applications.

Shallow-water benthic habitats of southwest Puerto Rico

This report describes the creation and assessment of benthic habitat maps for shallow-water (<30m) marine environments of the Guánica/Parguera and Finca Belvedere Natural Reserve in southwest Puerto Rico. The objective was to provide spatially-explicit information on the habitat types, biological cover and live coral cover of the region’s coral reef ecosystem. These fine-scale habitat maps, generated by interpretation of 2010 satellite imagery, provide an update to NOAA’s previous digital maps of the U.S. Caribbean (Kendall et al., 2001) for these areas. Updated shallow-water benthic habitat maps for the Guánica/Parguera region are timely in light of ongoing restoration efforts in the Guánica Bay watershed. The bay is served directly by one river, the Rio Loco, which flows intermittently and more frequently during the rainy season. The watershed has gone through a series of manipulations and alterations in past decades, mainly associated with agricultural practices, including irrigation systems, in the upper watershed. The Guánica Lagoon, previously situated to the north of the bay, was historically the largest freshwater lagoon in Puerto Rico and served as a natural filter and sediment sink prior to the discharge of the Rio Loco into the Bay. Following alterations by the Southwest Water Project in the 1950s, the Lagoon’s adjacent wetland system was ditched and drained; no longer filtering and trapping sediment from the Rio Loco. Land use in the Guánica Bay/Rio Loco watershed has also gone through several changes (CWP, 2008). Similar to much of Puerto Rico, the area was largely deforested for sugar cane cultivation in the 1800s, although reforestation of some areas occurred following the cessation of sugar cane production (Warne et al., 2005). The northern area of the watershed is generally mountainous and is characterized by a mix of forested and agricultural lands, particularly coffee plantations. Closer to the coast, the Lajas Valley Agricultural Reserve extends north of Guánica Bay to the southwest corner of the island. The land use practices and watershed changes outlined above have resulted in large amounts of sediment being distributed in the Rio Loco river valley (CWP, 2008). Storm events and seasonal flooding also transport large amounts of sediment to the coastal waters. The threats of upstream watershed practices to coral reefs and the nearshore marine environment have been gaining recognition. Guánica Bay, and the adjacent marine waters, has been identified as a “management priority area” by NOAA’s Coral Reef Conservation Program (CRCP, 2012). In a recent Guánica Bay watershed management plan, several critical issues were outlined in regards to land-based sources of pollution (LBSP; CWP, 2008). These include: upland erosion from coffee agriculture, filling of reservoirs with sediment, in-stream channel erosion, loss of historical Guánica lagoon, legacy contaminants and sewage treatment (CWP, 2008). The plan recommended several management actions that could be taken to reduce impacts of LBSP, which form the basis of Guánica watershed restoration efforts.

Atlas of the shallow-water benthic habitats of Majuro Atoll, Republic of the Marshall Islands

Digital maps of the shallow (<~30m deep) coral reef ecosystems of Majuro Atoll, Republic of the Marshall Islands, were created through visual interpretation of remote sensing imagery acquired between 2004 and 2006. Reef ecosystem features were digitized directly into a Geographic Information System. Benthic features were categorized according to a classification scheme with attributes including zone (location such as lagoon or forereef, etc.), structure (bottom type such as sand or patch reef, etc.) and percent hard bottom. This atlas consists of 27 detailed maps displaying reef zone and structure of coral ecosystems around Majuro. Adjacent maps in the atlas overlap slightly to ensure complete coverage. Maps and associated products can be used to support science and management activities on Majuro reef ecosystems including inventory, monitoring, conservation, and sustainable development applications. Maps are not to be used for navigation.

Synopsis of researcher meeting -- Bottlenose Dolphin Health & Risk Assessment Project, February 22-24, 2005

A meeting was convened on February 22-24, 2005 in Charleston, South Carolina to bring together researchers collaborating on the Bottlenose Dolphin Health and Risk Assessment (HERA) Project to review and discuss preliminary health-related findings from captured dolphins during 2003 and 2004 in the Indian River Lagoon (IRL), FL and Charleston (CHS), SC. Over 30 researchers with diverse research expertise representing government, academic and marine institutions participated in the 2-1/2 day meeting. The Bottlenose Dolphin HERA Project is a comprehensive, integrated, multi-disciplinary research program designed to assess environmental and anthropogenic stressors, as well as the health and long-term viability of Atlantic bottlenose dolphins (Tursiops truncatus). Standardized and comprehensive protocols are being used to evaluate dolphin health in the coastal ecosystems in the IRL and CHS. The Bottlenose Dolphin Health and Risk Assessment (HERA) Project was initiated in 2003 by Dr. Patricia Fair at the National Oceanic and Atmospheric Administration/National Ocean Service/Center for Coastal Environmental Health and Biomolecular Research and Dr. Gregory Bossart at the Harbor Branch Oceanographic Institution under NMFS Scientific Research Permit No. 998-1678-00 issued to Dr. Bossart. Towards this end, this study focuses on developing tools and techniques to better identify health threats to these dolphins, and to develop links to possible environmental stressors. Thus, the primary objective of the Dolphin HERA Project is to measure the overall health and as well as the potential health hazards for dolphin populations in the two sites by performing screening-level risk assessments using standardized methods. The screening-level assessment involves capture, sampling and release activities during which physical examinations are performed on dolphins and a suite of nonlethal morphologic and clinicopathologic parameters, to be used to develop indices of dolphin health, are collected. Thus far, standardized health assessments have been performed on 155 dolphins during capture-release studies conducted in Years 2003 and 2004 at the two sites. A major collaboration has been established involving numerous individuals and institutions, which provide the project with a broad assessment capability toward accomplishing the goals and objectives of this project.

Fijian coral reef damage and recovery from Cyclone Kina

EXTRACT (SEE PDF FOR FULL ABSTRACT): Early in 1993, Cyclone Kina struck the Fiji Islands, causing more than $100 million in property damage and damaging the coral environment as well. A few days after the cyclone, the most damaged reef was studied. The same reef had been studied 6 months before. This reef crest is dominated by Acropora. Comparison showed that 80-90% of the Acropora was torn from the outer reef and deposited in the inner lagoon. ... It is estimated that it will take a few years to 30 years for the reef to recover to pre-Kina conditions.

Generating power from waves on a breakwater.

Outlines the possibility for wave power generation at artificial islands by construction of a breakwater. Reviews the development of wave energy systems, and describes several wave generators, e.g. the Mauritius lagoon system, the Nodding Duck, the oscillating cylinder, the oscillating water column and the Lancaster Bag. Applications and costs are outlined. (C.J.U.)

Brackish-water fishery resources

Ceylon has about 300,000 acres of coastal brackish-water areas of which about 100,000 acres constitute shallow lagoons, tidal flats, mangrove swamps and saline marshes, and the rest deeper lagoons and estuaries. While the former represent a vast potential resource with regard to fish farming, the latter are the sites of important fisheries. W. H. Schuster (1951) estimated the average natural production of Ceylon’s brackish waters to be less than 20 lbs. per acre per annum. Since then estimates have been made by the author for a rich lagoon, the Negombo lagoon, a poorly productive lagoon, the Ratgama lake (Dodanduwa) and studies are in progress of some of the other lagoons. The natural production of the Ratgama lake was estimated in 1959 to be 18.5 lbs. per acre per annum while that of Negombo lagoon was estimated in 1960 to be 65 lbs. per acre per annum. It is reasonable to estimate the average production of Ceylon's brackish-waters to be 25 lbs. per acre per annum. Thus the total production is about 3,350 tons per annum. Considering the fact that the island's present total production is 90,000 tons per annum, the brackish-waters contribute 3.7% of it. Schuster (1951) further states that the natural production in the brackish-waters of other countries is around 80 lbs. per acre per annum. In order to increase our average natural production to this value it would seem necessary to consider the nature, biology and fish resources of the brackish-waters and draw some conclusions with regard to their proper exploitation.

A preliminary report on Chanos fry surveys carried out in the brackish water areas of Mannar, Puttalam and Negombo

This paper records the results of the Chanos fry surveys carried out in Mannar, Puttalam and Negombo lagoon areas since 1967, and gives a summary of the earlier surveys. The maximum amount of Chanos fry was obtained from Mannar area during April and May. All three areas have their main commercial fry season in April and May with a second commercially negligible season in October–November. The quantity of fry collected has been utilized to evaluate the potential fry production figures. Mannar 400,000,000 fry/annum. Puttalam 200,000,000 fry/annum. It would be extremely difficult to estimate the number of eggs laid, the larvae hatched and the fry available each year along the coastal areas of Ceylon. It is necessary to obtain biological, meteorological and oceanographical data so as to provide a basis for the prediction and estimation of fry populations in the coastal waters of Ceylon. The suggested ideal season for commercial catches of Chanos fry is April–May with operations to be carried out simultaneously all along the west coast.

Studies on the seasonal cycle of sea surface temperatures, salinities, oxygen and phytoplankton in Koddiyar Bay, Trincomalee in relation to the shore seine fishery

In Sri Lanka waters, Durairatnam (1963, 1969) had reported on the seasonal variation of plankton at Puttalam lagoon, Dutch Bay, Portugal Bay and in the inshore and off shore waters off Colombo. Similar investigations were carried out at Koddiyar Bay especially in the estuarine waters where the three tributaries of the river Mahaveli empty their waters into the bay. The estuary is perennial. The fishes constituting the estuarine fisheries are mostly shallow water marine species which can tolerate considerable variations of salinities. Prawns and crabs also constitute a very valuable fishery in estuarine waters.

A baseline report of water quality and invertebrate assessment in River Hohwa, Lake Albert: final report

Tullow Oil plc is to launch an onshore Early Production System (EPS) of oil drilling rated at 4,000 barrels of oil per day by 2009. The location of the EPS is in the Kaiso-Tonya area of Block 2 Oil Exploration Zone along Lake Albert within the Albertine graben. Tullow Oil plc contracted Environmental Resources Management (ERM) Southern Africa (Pty) Ltd in conjunction with Environmental Assessment Consult Limited (EACL) to undertake an Environmental Impact Assessment (EIA) for pre-construction and operation of the proposed EPS. ERM in association with EACL requested National Fisheries Resources Research Institute (NaFIRRI) to conduct a baseline survey of water quality and invertebrates in River Hohwa. This study was requested as part of an earlier baseline survey conducted at the Kaiso-Ngassa spit oil exploration area in Block 2. It was conducted at five selected sites (Fig. 1 & Table 1) within the Hohwa River basin in the Kaiso-Tonya Exploration Area 2. The study was pertinent because the targeted oil wells for EPS are upstream this river which drains the Kaiso-Ngassa valley into Ngassa lagoon.

A baseline fish survey and water quality assessment of Butiaba-Wanseko area on Lake Albert

This report gives the baseline information specifically on water quality (physical/chemical, nutrient and phytoplankton biomass status) of the open part of the lake, a river flowing into the lake and a lagoon, within Butiaba-Wanseko area. Oil and gas exploration in the Albertine Graben are on-going activities by Tullow Oil Plc, and Heritage Oil and Gas (Heritage). Part of the activities involve 2-D TZ seismic surveys in exploration area Block 2 (Butiaba-Wanseko area) in Buliisa District. A study of the transition zone (basically along the shoreline was undertaken by National Fisheries Resources Research Institute (NaFIRRI) on behalf of Environmental Assessment Consult Ltd (EACL) during September 2007. A major objective of the study was to carry out a baseline survey of the fisheries and water quality of the lake shore between Wanseko and Butiaba prior to the proposed 2-D Seisimic investigation.

Feasibility study of biofuel production from freshwater fern, Azolla sp in Anzali Wetland

Production of bioethanol through acidic and enzymatic hydrolysis of aquatic Azolla sp., as a new source of bio-mass, has been performed, as a means to control increasing growth and reducing undesirable effects of this plant in Anzali lagoon. After sampling, drying and crushing, Azolla was hydrolyzed, using diluted acid and enzyme. Diluted acid hydrolysis was done using both autoclave and a high-pressure system (Batch Synth® Microwave synthesizer). The effects of temperature and time (in autoclave) and concentration of acid (in both) were compared. Cellubrix®, a ommercial cellulase source, was used for enzymatic hydrolysis process. The amounts of reducing sugars, glucose and furfural, released from hydrolyzate, were measured. To produce alcohol, Sacchromyces cerevisiae (to ferment sixcarbon sugars), Zygowilliopsis californica and Pichia stipitis (to ferment five-carbon and sixcarbon sugars) were used. Maximum amounts of glucose (4.83% w/w) and reducing sugars (14.15% w/w) were obtained using acid hydrolysis in autoclave. In the microwave oven, maximum glucose (5.04% w/w) and reducing sugars (13.27 w/w) were obtained at 180 and 200 °C, respectively. Under these conditions, maximum produced furfural was 1.54 g/L. The difference between amounts of furfural obtained from acid hydrolysis of Azolla in microwave oven compared to autoclave was statistically significant. Amounts of alcohol produced and its yields were 3.99 g/L and 33.13% for S. cerevisiae in 48 hours, 3.73 g/L and 30.45% for Pichia stipites in 48 hours, and 3.73 g/L and 30.45% for Z. californica in 24 hours after inoculation, respectively, with significant differences. Statistical comparison of results showed significant differences (P<0.05) in glucose production, at different conditions. Amounts of reducing sugars and glucose increased after optimization of levels of acid, time, and temperature. The overall optimum released sugar and glucose were obtained with 1.67% (w/v) acid using autoclave. Higher temperatures in microwave oven caused a significant increase (P<0.05) in furfural. Furfural severely inhibits fermentation. Hence, regarding the issues of energy consumption and time, amounts of inhibiting substances and sugar production, autoclave is found to be superior to the high temperature and pressure, generated in microwave oven, for hydrolyzing Azolla. Furthermore, given the amounts of Azolla in Anzali lagoon, it may be recommendable to use this plant as a biomass resource.

Determination of bioaccumulation and microscopic survey of LAS pollutant effect on the liver, kidney and gill tissues of Rutilus frisii kutum in Caspian sea

Linear alkylbenzene sulfonate (LAS) are widely used in detergent industry. Due to contaminants entering the water, and the effects of their accumulation in fish, LAS, has a great importance in environmental pollution. In the present study, accumulation of LAS and its histological effects on gill tissue, liver and kidney of Caspian kutum (Rutilus frisii kutum) were studied. Caspian kutum is the most important and most valuable teleosts of the Caspian Sea. Due to releasing Caspian Kutum in rivers and Anzali Lagoon and unlimited entry of wastewater to the aquatic ecosystem, research on the impact of LAS on Caspian kutum is important. In the present study, fish exposed to sublethal concentrations of LAS (0.58, 1.16 and 2.32 mg/l) for 192 hours. Control treatments with three replicates at 0, 24, 48, 72, 96 and 192 hours were done. For assessments of the histological effects of LAS, tissue sections prepared and by using Hematoxylin - Eosin were stained, then the prepared sections, examined by light microscopy. For determination of the bio accumulation of LAS, the soxhlet extraction and solid phase extraction was performed to determine the amount of LAS using HPLC with fluorescence detector. According to results average of bioconcentration factor and LAS concentrations in fish had reached stable levels after approximately 72 h and thus represented steady state BCF values in this species. The value of steady-state bio-concentration factor of total LAS was 33.96 L.Kg- 1 and for each of the homologous C10-n-LAS, C11-n-LAS, C12-n-LAS and C13-n- LAS were 3.84, 6.15, 8.58 and 15.57 L.Kg-1 respectively. According to the results obtained in gills exposed to LAS, histopathological alteration include hypertrophy, lifting of lamella epithelium, edema, clubbing of lamellae hyperplasia, lamellar fusion and aneurysm were seen. In liver tissue exposed to three concentrations of LAS, congestion and dilation of sinusoids, irregular-shaped nuclei and degeneration in the hepatocyte, vacuolar degeneration and necrosis were observed. In kidney exposed to three concentrations of LAS, reduction of the interstitial haematopoietic tissue, degeneration in the epithelial cells of renal tubule, tubular degeneration, necrosis, shrinkage and luminal occlusion were observed. According to the results the most alteration due to exposure to LAS was seen in the gill tissue. None of the control samples showed histological effects of LAS.