Gestational drive and the green-bearded placenta.

A "green beard" refers to a gene, or group of genes, that is able to recognize itself in other individuals and direct benefits to these individuals. Green-beard effects have been dismissed as implausible by authors who have implicitly assumed sophisticated mechanisms of perception and complex behavioral responses. However, many simple mechanisms for genes to "recognize" themselves exist at the maternal-fetal interface of viviparous organisms. Homophilic cell adhesion molecules, for example, are able to interact with copies of themselves on other cells. Thus, the necessary components of a green-beard effect -- feature, recognition, and response -- can be different aspects of the phenotype of a single gene. Other green-beard effects could involve coalitions of genes at closely linked loci. In fact, any form of epistasis between a locus expressed in a mother and a closely linked locus expressed in the fetus has the property of "self-recognition." Green-beard effects have many formal similarities to systems of meiotic drive and, like them, can be a source of intragenomic conflict.

Mutagenesis and gene identification in Dictyostelium by shotgun antisense.

We have developed a mutagenesis technique that uses antisense cDNA to identify genes required for development in Dictyostelium discoideum. We transformed Dictyostelium cells with a cDNA library made from the mRNA of vegetative and developing cells. The cDNA was cloned in an antisense orientation immediately downstream of a vegetative promoter, so that in transformed cells the promoter will drive the synthesis of an antisense RNA transcript. We find that individual transformants typically contain one or occasionally two antisense cDNAs. Using this mutagenesis technique, we have generated mutants that fail to aggregate, aggregate but fail to form fruiting bodies, or aggregate but form abnormal fruiting bodies. The individual cDNA molecules from the mutants were identified and cloned using PCR. Initial sequence analysis of the PCR products from 35 mutants has identified six novel Dictyostelium genes, each from a transformant with one antisense cDNA. When the PCR-isolated antisense cDNAs were ligated into the antisense vector and the resulting constructs transformed into cells, the phenotypes of the transformed cells matched those of the original mutants from which each cDNA was obtained. We made homologous recombinant gene disruption transformants for three of the novel genes, in each case generating mutants with phenotypes indistinguishable from those of the original antisense transformants. Shotgun antisense thus is a rapid way to identify genes in Dictyostelium and possibly other organisms.

Examining the University of Denver's Family Friendly Benefits Plans: A look at Creating More Family Friendly Benefits to Drive Employee Retention

In the latter half of the twentieth century the workforce dynamic changed when the number of women entering the workforce increased by record amounts. In direct opposition to this change was the inability of organizations to meet the needs of employees with childcare concerns. Organizations and employees alike are best served when policies, procedures, and benefits are implemented to achieve a positive work/life balance. Companies that institute benefits that are supportive to families observe decreases in turnover and increased employee retention. Employees who are offered family friendly resources have been known to stay with companies even when offered a higher salary elsewhere. Demonstrating that retention of valued employees is linked to an organizations ability to offer support for family needs.

Arguments prepared by Daniel Appleton White for meetings of Phi Beta Kappa, 1796-1797

Three handwritten arguments prepared by Phi Beta Kappa member Daniel Appleton White (1776-1861; Harvard AB 1797) for discussion at meetings of the Alpha chapter at Harvard University in 1796 and 1797. The documents consist of a small paper notebook with a response to the prompt, "Whether the deeper studies, such as metaphysics, mathematics & natural philosophy, are entitled to our chief attention?" dated September 27, 1796, and prepared for debate with classmate Isaac Wellington (died 1797); a one-leaf document with a disputation on, "Whether civilized nations have a right to drive uncivilized nations from the lands they occupy?" dated December 8, 1796; and a small paper notebook containing White’s argument to the prompt, "Would a national university be beneficial for America?" that he debated with John Collins Warren (1778-1856; Harvard AB 1797) during the chapter’s May 16, 1797, meeting.

Formerly utilized MED/AEC sites remedial action program : radiological survey of the Museum of Science and Industry, 57th Street and Lake Shore Drive, Chicago, Illinois /

Final report, Feb. 1979.

Evaluation of the 2006 Labor Day You Drink & Drive, You Lose, Campaign : July 24 - September 17, 2006.

"February 2007"

Participation in a household hazardous waste collection drive and "before" and "after" public knowledge and disposal practices : Champaign County : technical report /

"HWRIC RR-025."

Power for operating machinery in printing houses, a treatise on the methods of applying power to printing presses and allied machinery, with particular reference to electric drive./

Mode of access: Internet.

Technical manual, operator and organizational manual : grader, road, motorized, DED, type 1, 6 wheel, 4 wheel drive, front wheel steering, 12-foot blade, Caterpillar model 120, FSN 3805-466-0084.

Includes index.

Plan showing grades and drains for drive and garden, on grounds of Mr. J. J. Reimers. Davenport, Iowa.

Red, black ink on tracing paper; contour lines, elevations; arbors, steps; plan, profiles of grades; signed. 88x58 cm. Scale: 1"=20' [from photographic copy by Lance Burgharrdt]

Detail plan of pool. Estate of G.M. Reynolds. Lake Shore Drive

Red, blue, black in on linen; plan, cross-section; signed; 53 x 47 cm; Scale: 1" = 1' [from photographic copy by Lance Burgharrdt]

Estate of E.F. Simms, Esq....Plan of topo. survey for entrance drive, #2.

Pencil, red, black ink on linen; elevations, distances; unsigned; 83 x 54 cm.; Scale: 1" = 60' [from photographic copy by Lance Burgharrdt]