Movement patterns of winter flounder (Pseudopleuronectes americanus) in the southern Gulf of Maine: observations with the use of passive acoustic telemetry

Despite its recreational and commercial importance, the movement patterns and spawning habitats of winter flounder (Pseudopleuronectes americanus) in the Gulf of Maine are poorly understood. To address these uncertainties, 72 adult winter flounder (27–48 cm) were fitted with acoustic transmitters and tracked by passive telemetry in the southern Gulf of Maine between 2007 and 2009. Two sympatric contingents of adult winter flounder were observed, which exhibited divergent spawning migrations. One contingent remained in coastal waters during the spawning season, while a smaller contingent of winter flounder was observed migrating to estuarine habitats. Estuarine residence times were highly variable, and ranged from 2 to 91 days (mean=28 days). Flounder were nearly absent from the estuary during the fall and winter months and were most abundant in the estuary from late spring to early summer. The observed seasonal movements appeared to be strongly related to water temperature. This is the first study to investigate the seasonal distribution, migration, and spawning behavior of adult winter flounder in the Gulf of Maine by using passive acoustic telemetry. This approach offered valuable insight into the life history of this species in nearshore and estuarine habitats and improved the information available for the conservation and management of this species.

Use of stereo camera systems for assessment of rockfish abundance in untrawlable areas and for recording pollock behavior during midwater trawls

We describe the application of two types of stereo camera systems in fisheries research, including the design, calibration, analysis techniques, and precision of the data obtained with these systems. The first is a stereo video system deployed by using a quick-responding winch with a live feed to provide species- and size- composition data adequate to produce acoustically based biomass estimates of rockfish. This system was tested on the eastern Bering Sea slope where rockfish were measured. Rockfish sizes were similar to those sampled with a bottom trawl and the relative error in multiple measurements of the same rockfish in multiple still-frame images was small. Measurement errors of up to 5.5% were found on a calibration target of known size. The second system consisted of a pair of still-image digital cameras mounted inside a midwater trawl. Processing of the stereo images allowed fish length, fish orientation in relation to the camera platform, and relative distance of the fish to the trawl netting to be determined. The video system was useful for surveying fish in Alaska, but it could also be used broadly in other situations where it is difficult to obtain species-composition or size-composition information. Likewise, the still-image system could be used for fisheries research to obtain data on size, position, and orientation of fish.

Use of pop-up satellite archival tag technology to study postrelease survival of and habitat use by estuarine and coastal fishes: an application to striped bass (Morone saxatilis)

Pop-up satellite archival tags (PSATs) have been used to study movements, habitat use, and postrelease survival of large pelagic vertebrates, but the size of these tags has historically precluded their use on smaller coastal species. To evaluate a new generation of smaller PSATs for the study of postrelease survival and habitat use of coastal species, we attached Microwave Telemetry, Inc., X-tags to ten striped bass (Morone saxatilis) 94–112 cm total length (TL) caught on J hooks and circle hooks during the winter recreational fishery in Virginia. Tags collected temperature and depth information every five minutes and detached from the fish after 30 days. Nine of the ten tags released on schedule and eight transmitted 30% to 96% (mean 78.6%) of the archived data. Three tags were physically recovered during or after the transmission period, allowing retrieval of all archived data. All eight striped bass whose tags transmitted data survived for 30 days after release, including two fish that were hooked deeply with J hooks. The eight fish spent more than 90% of their time at depths less than 10 m and in temperatures of 6–9°C, demonstrated no significant diel differences in depth or temperature utilization (P>0.05), and exhibited weak periodicities in vertical movements consistent with daily and tidal cycles.

Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles

Self-amplifying RNA or RNA replicon is a form of nucleic acid-based vaccine derived from either positive-strand or negative-strand RNA viruses. The gene sequences encoding structural proteins in these RNA viruses are replaced by mRNA encoding antigens of interest as well as by RNA polymerase for replication and transcription. This kind of vaccine has been successfully assayed with many different antigens as vaccines candidates, and has been shown to be potent in several animal species, including mice, nonhuman primates, and humans. A key challenge to realizing the broad potential of self-amplifying vaccines is the need for safe and effective delivery methods. Ideally, an RNA nanocarrier should provide protection from blood nucleases and extended blood circulation, which ultimately would increase the possibility of reaching the target tissue. The delivery system must then be internalized by the target cell and, upon receptor-mediated endocytosis, must be able to escape from the endosomal compartment into the cell cytoplasm, where the RNA machinery is located, while avoiding degradation by lysosomal enzymes. Further, delivery systems for systemic administration ought to be well tolerated upon administration. They should be safe, enabling the multiadministration treatment modalities required for improved clinical outcomes and, from a developmental point of view, production of large batches with reproducible specifications is also desirable. In this review, the concept of self-amplifying RNA vaccines and the most promising lipid-based delivery systems are discussed.

Validation of back-calculation equations for juvenile bluefish (Pomatomus saltatrix) with the use of tetracycline-marked otoliths

In recent years, a decrease in the abundance of bluefish (Pomatomus saltatrix) has been observed (Fahay et al., 1999; Munch and Conover, 2000) that has led to increased interest in a better understanding the life history of the species. Estimates of several young-of-the-year (YOY) life history characteristics, including the importance and use of estuaries as nursery habitat (Kendall and Walford, 1979) and size-dependant mortality (Hare and Cowen, 1997), are reliant upon the accuracy of growth determination. By using otoliths, it is possible to use back-calculation formulae (BCFs) to estimate the length at certain ages and stages of development for many species of fishes. Use of otoliths to estimate growth in this way can provide the same information as long-term laboratory experiments and tagging studies without the time and expense of rearing or recapturing fish. The difficulty in using otoliths in this way lies in validating that 1) there is constancy in the periodicity of the increment formation, and 2) there is no uncoupling of the relationship between somatic and otolith growth. To date there are no validation studies demonstrating the relationship between otolith growth and somatic growth for bluefish. Daily increment formation in otoliths has been documented for larval (Hare and Cowen, 1994) and juvenile bluefish (Nyman and Conover, 1988). Hare and Cowen (1995) found ageindependent variability in the ratio of otolith size to body length in early age bluefish, although these differences varied between ontogenetic stages. Furthermore, there have been no studies where an evaluation of back-calculation methods has been combined with a validation of otolithderived lengths for juvenile bluefish.

The use of multibeam sonar mapping techniques to refine population estimates of the endangered white abalone (Haliotis sorenseni)

Multibeam sonar mapping techniques provide detailed benthic habitat information that can be combined with the data on species-specific habitat preferences to provide highly accurate calculations of populations in a particular area. The amount of suitable habitat available for the endangered white abalone (Haliotis sorenseni) was quantified to aid in obtaining an accurate estimate of the number of remaining individuals at two offshore banks and one island site off the coast of southern California. Habitat was mapped by using multibeam sonar survey techniques and categorized by using rugosity and topographic position analysis. Abalone densities were evaluated by using a remotely operated vehicle and video transect methods. The total amount of suitable habitat at these three sites was far greater than that previously estimated. Therefore, although present estimates of white abalone densities are several orders of magnitude lower than historic estimates, the total population is likely larger than previously reported because of the additional amount of habitat surveyed in this study.

Use of a fishery independant index to predict recruitment and catches of the spiny lobster

This paper presents a review of recruitment and catch predictions based on an index of abundance of juveniles and pre-recruits (fishery independent index) in the Cuban lobster fisheries. This methodology can provide information based on fisheries data that can improve the management of the fishery.

Preliminary use of oxygen stable isotopes and the 1983 El Niño to assess the accuracy of aging black rockfish (Sebastes melanops)

Black rockfish (Sebastes melanops) range from California to Alaska and are found in both nearshore and shallow continental shelf waters (Love et al., 2002). Juveniles and subadults inhabit shallow water, moving deeper as they grow. Generally, adults are found at depths shallower than 55 meters and reportedly live up to 50 years. The species is currently managed by using information from an age-structured stock assessment model (Ralston and Dick, 2003).

Biology and Potential Use of Pacific Grenadier, Coryphaenoides acrolepis, off California

Grenadiers (family Macrouridae) are the most abundant fish on most continental slope areas worldwide. Off California the Pacific grenadier, Coryphaenoides acrolepis, occurs in relatively large numbers and may have marketing potential. This repon provides information on the biology of the species and catch results from a number of scientific cruises. Catch data on several other species found together with Pacific grenadier, panicularly sablefish, Anoplopoma fimbria, are also given. The fish were caught with a bottom trawl (15 trawls), and with free-vehicle longline gear (117 sets). The latter was a hook and line system in which the gear was dropped to the seafloor untethered to the fishing vessel, and floated to the surface, with the catch, when detachable weights were automatically released. Sablefish dominated longline catches in depths of 200-600fm (334-1,098m), while Pacific grenadier was most abundant between 600 and 1,OOOfm (1,098-1,830m). Best trawl catches of Pacific grenadier were made at depths between 615 and 675fm (1,125 and 1,235 m) and at 760fm (1,391 m). Ripe females were absent from our samples, but spent females were found during the entire year with highest numbers in the spring and early summer. Only one larva was found despite extensive sampling with plankton nets. Pacific grenadier was found to have good edible qualities by a taste-test panel, although the protein content (15 percent) and flesh yield (24 percent) were significantly lower than those of other fishes. A second species, the giant grenadier, Albatrossia pectoralis, was found to have exceptionally poor eating qualities and even lower protein content.

Use of Fish Corrals in the Seine Fishery of the Virgin Islands

Although selected aspects of the commercial fishery in the Virgin Islands have been documented since the early 1930's (Fiedler and Jarvis, 1932; Idyll and Randall, 1959; Hess, 1961; Swingle et al. 1970; Brownell, 1971; Brownell and Rainey, 1971; Sylvester and Dammann, 1972, and Olsen et al., 1978), fish corrals and their use have not been described. This account, based on personal observations made during 1985-86, summarizes commercial fishing methods in the Virgin Islands (U. S. and British), documents the use of fish corrals, and serves as an introduction to the methodologies of this harvesting technique. Interviews of commercial fishermen about how and when fish corrals are used provided information not available from direct observation. Local common names for gear type and fish species are shown in parentheses.

EXPERIMENTAL TECHNIQUES IN COMBUSTION USE OF IN-CYLINDER IONISATION FOR THE INVESTIGATION AND CONTROL OF SPARK IGNITION ENGINES.

Ions generated during combustion have been used in three ways to give qualitative combustion information. Langmuir type probes have been inserted into the combustion chamber opposite the spark plug location. The centre electrode of the sparking plug itself has been used to produce an ionisation signal from the slightly ionised gases remaining after the flame front has departed. The spark discharge at ignition time has been used as an anemometer.

The Nile perch fishery: traditional and emerging fisheries, overfishing and the use of illegal gears on Lake Albert

The fisheries of Lake Albert have come under increasing focus due to several driving forces that have synergistically evolved resulting into concerns from diverse stakeholders. The driving forces include: the commercialization of the fisheries with entry into the value chain of industrial fish processing, a decline in fish stocks especially of the large-size fishes and the emergency of the light - fishing targeting small pelagic fishes. In addition, the assumption by some opinion leaders that light-fishing (use of light) has destroyed the Nile perch fishery of Lake Albert, other factors such as cross-border fishing conflicts, the emergence of oil, an influx of traders in fish-related activities, and the limited regulatory and enforcement regimes for the diverse commercially exploited fish fauna of Lake Albert all require continuous information and action.

The Impact of Contract Type on Service Provider Information Requirements

This paper is concerned with the role of information in the servitization of manufacturing which has led to “the innovation of an organisation’s capabilities and processes as equipment manufacturers seek to offer services around their products” (Neely 2009, Baines et al 2009). This evolution has resulted in an information requirement (IR) shift as companies move from discrete provision of equipment and spare parts to long-term service contracts guaranteeing prescribed performance levels. Organisations providing such services depend on a very high level of availability and quality of information throughout the service life-cycle (Menor et al 2002). This work focuses on whether, for a proposed contract based around complex equipment, the Information System is capable of providing information at an acceptable quality and requires the IRs to be examined in a formal manner. We apply a service information framework (Cuthbert et al 2008, McFarlane & Cuthbert 2012) to methodically assess IRs for different contract types to understand the information gap between them. Results from case examples indicate that this gap includes information required for the different contract types and a set of contract-specific IRs. Furthermore, the control, ownership and use of information differs across contract types as the boundary of operation and responsibility changes.

Use of contexts in language model interpolation and adaptation

Language models (LMs) are often constructed by building multiple individual component models that are combined using context independent interpolation weights. By tuning these weights, using either perplexity or discriminative approaches, it is possible to adapt LMs to a particular task. This paper investigates the use of context dependent weighting in both interpolation and test-time adaptation of language models. Depending on the previous word contexts, a discrete history weighting function is used to adjust the contribution from each component model. As this dramatically increases the number of parameters to estimate, robust weight estimation schemes are required. Several approaches are described in this paper. The first approach is based on MAP estimation where interpolation weights of lower order contexts are used as smoothing priors. The second approach uses training data to ensure robust estimation of LM interpolation weights. This can also serve as a smoothing prior for MAP adaptation. A normalized perplexity metric is proposed to handle the bias of the standard perplexity criterion to corpus size. A range of schemes to combine weight information obtained from training data and test data hypotheses are also proposed to improve robustness during context dependent LM adaptation. In addition, a minimum Bayes' risk (MBR) based discriminative training scheme is also proposed. An efficient weighted finite state transducer (WFST) decoding algorithm for context dependent interpolation is also presented. The proposed technique was evaluated using a state-of-the-art Mandarin Chinese broadcast speech transcription task. Character error rate (CER) reductions up to 7.3 relative were obtained as well as consistent perplexity improvements. © 2012 Elsevier Ltd. All rights reserved.