983 resultados para Transmission of microorganisms


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Protecting signals is one of the main tasks in information transmission. A large number of different methods have been employed since many centuries ago. Most of them have been based on the use of certain signal added to the original one. When the composed signal is received, if the added signal is known, the initial information may be obtained. The main problem is the type of masking signal employed. One possibility is the use of chaotic signals, but they have a first strong limitation: the need to synchronize emitter and receiver. Optical communications systems, based on chaotic signals, have been proposed in a large number of papers. Moreover, because most of the communication systems are digital and conventional chaos generators are analogue, a conversion analogue-digital is needed. In this paper we will report a new system where the digital chaos is obtained from an optically programmable logic structure. This structure has been employed by the authors in optical computing and some previous results in chaotic signals have been reported. The main advantage of this new system is that an analogue-digital conversion is not needed. Previous works by the authors employed Self-Electrooptical Effect Devices but in this case more conventional structures, as semiconductor laser amplifiers, have been employed. The way to analyze the characteristics of digital chaotic signals will be reported as well as the method to synchronize the chaos generators located in the emitter and in the receiver.

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A new proposal to have secure communications in a system is reported. The basis is the use of a synchronized digital chaotic systems, sending the information signal added to an initial chaos. The received signal is analyzed by another chaos generator located at the receiver and, by a logic boolean function of the chaotic and the received signals, the original information is recovered. One of the most important facts of this system is that the bandwidth needed by the system remain the same with and without chaos.

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For analyzing the mechanism of energy transduction in the “motor” protein, myosin, it is opportune both to model the structural change in the hydrolytic transition, ATP (myosin-bound) + H2O → ADP⋅Pi (myosin-bound) and to check the plausibility of the model by appropriate site-directed mutations in the functional system. Here, we made a series of mutations to investigate the role of the salt-bridge between Glu-470 and Arg-247 (of chicken smooth muscle myosin) that has been inferred from crystallography to be a central feature of the transition [Fisher, A. J., Smith, C. A., Thoden, J. B., Smith, R., Sutoh, K., Holden, H. M., & Rayment, I. (1995) Biochemistry 34, 8960–8972]. Our results suggest that whether in the normal, or in the inverted, direction an intact salt-bridge is necessary for ATP hydrolysis, but when the salt-bridge is in the inverted direction it does not support actin activation. Normally, fluorescence changes result from adding nucleotides to myosin; these signals are reported by Trp-512 (of chicken smooth muscle myosin). Our results also suggest that structural impairments in the 470–247 region interfere with the transmission of these signals to the responsive Trp.

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Adult Xenopus laevis frogs made transgenic by restriction enzyme-mediated integration were bred to test the feasibility of establishing lines of frogs that express transgenes. All of the 19 animals raised to sexual maturity generated progeny that expressed the transgene(s). The patterns and levels of expression of green fluorescent protein transgenes driven by a viral promoter, rat promoter, and four X. laevis promoters were all unaffected by passage through the germ line. These results demonstrate the ease of establishing transgenic lines in X. laevis.