A small bispecific antibody construct expressed as a functional single-chain molecule with high tumor cell cytotoxicity.

Construction of a bispecific single-chain antibody derivative is described that consists of two different single-chain Fv fragments joined through a Gly-Ser linker. One specificity of the two Fv fragments is directed against the CD3 antigen of human T cells and the other is directed against the epithelial 17-1A antigen; the latter had been found in a clinical trial to be a suitable target for antibody therapy of minimal residual colorectal cancer. The construct could be expressed in CHO cells as a fully functional protein, while its periplasmic expression in Escherichia coli resulted in a nonfunctional protein only. The antigen-binding properties of the bispecific single-chain antibody are indistinguishable from those of the corresponding univalent single-chain Fv fragments. By redirecting human peripheral T lymphocytes against 17-1A-positive tumor cells, the bispecific antibody proved to be highly cytotoxic at nanomolar concentrations as demonstrated by 51Cr release assay on various cell lines. The described bispecific construct has a molecular mass of 60 kDa and can be easily purified by its C-terminal histidine tail on a Ni-NTA chromatography column. As bispecific antibodies have already been shown to be effective in vivo in experimental tumor systems as well as in phase-one clinical trials, the small CD3/17-1A-bispecific antibody may be more efficacious than intact antibodies against minimal residual cancer cells.

(Table 3) Noble gas composition of dissociated gas hydrate specimens from ODP Leg 164 sites

Fractionation of the noble gases should occur during formation of a Structure I gas hydrate from water and CH4 such that CH4 hydrate is greatly enriched in Xenon. Noble gas concentrations and fractionation factors (F[4He], F[22Ne], F[86Kr], and F[132Xe] as well as R/Ra) were determined for eight gas hydrate specimens collected on Leg 164 to evaluate this theoretical possibility and to assess whether sufficient quantities of Xe are hosted in oceanic CH4 hydrate to account for Xe "missing" from the atmosphere. The simplest explanation for our results is that samples contain mixtures of air and two end-member gases. One of the end-member gases is depleted in Ne, but significantly enriched in Kr and Xe, as anticipated if the source of this gas involves fractionation during Structure I gas hydrate formation. However, although oceanic CH4 hydrate may be greatly enriched in Xe, simple mass balance calculations indicate that oceanic CH4 hydrate probably represents only a minor reservoir of terrestrial Xe. Noble gas analyses may play an important role in understanding the dynamics of gas hydrate reservoirs, but significantly more work is needed than presented here.

Ann Arbor and Superior Township (Mich.) residences, ca. 1874

Res. of E. C. Seaman, Esq., Ann Arbor, Mich.; Res. of Henry Krause, esq., Ann Arbor, Mich.; Res. of J. O. Thompson (na3778) Sec 29, Superior Tp. Mich.; Res. of Frederick Schmidt, Firm of Mack & Schmidt, Ann Arbor, Mich.; Res. of John W. Nanry, Sec. 8 Supiror [sic] Tp. Mich.; Res. of C. H. Millen, Ann Arbor, Mich.

University of Michigan Football Team, 1904

Top Row: assistant coach William Cole, Ted Stuart, James De Pree, Paul Magoffin, Walter Graham, Harry Patrick,

Middle Row: Hal Weeks, Harry Hammond, Thomas Jefferson Smull, Charles Briggs, Adolph "Germany" Schulz, Jay Mack Love, John Garrels, William Dennison Clark, Walter Rheinschild, Edward Hammond, Walter Becker

Front Row: trainer Keene Fitzpatrick, Tom Hammond, John Curtis, Henry Schulte, captain Willie Heston, , Frank Longman, Charles Carter, coach Fielding Yost, student mngr. Albert H. Montgomery

Seated in front: Fred Norcross

U-M Softball Team, 2000

Front Row: Karmen Lappo, Jamie Gillies, Melissa Gentile (co-captain), Pam Kosanke (co-captain),

Second Row: Kate Eiland, Marissa Young, Courtney Murdock, Melinda Moulden, Meghan Doe, Marie Barda, Mary Conner.

Third Row: Liz Elsner, Chrissy Garza, Rebecca Tune, Kelsey Kollen, Stefanie Volpe,

Top Row: Melissa Taylor, Lisa Mack, Kim Bugel.

Large school bus design vehicle dimensions.

Arkansas State Highway and Transportation Department, Little Rock

School bus acceleration characteristics.

Arkansas State Highway and Transportation Department, Little Rock

The history of the adventures of Joseph Andrews and of his friend Mr. Abraham Adams, written in imitation of the manner of Cervantes, author of Don Quixote.

Mode of access: Internet.

Mister Scoggins' Saturday night,

Mode of access: Internet.

Sorghum movements in the United States : interregional flow patterns and transportation requirements in 1977 /

Includes bibliographical references.

Attraction /

Mode of access: Internet.

The popular piano collection. [music].

Illustrated t.p.

Automotive fuel economy and emissions experimental data. Final report.

Transportation Systems Center, Cambridge, Mass.