Bioprospecção de extratos metanólicos de macroalgas marinhas do litoral do Rio Grande do Norte: avaliação das atividades anticoagulante, antioxidante e antiproliferativa

Seaweeds are organisms known to exhibit a variety of biomolecules with pharmacological properties. The coast of Rio Grande do Norte has over 100 species of seaweeds, most of them not yet explored for their pharmacological potential. Sugars and phenolic compounds are the most studied of these being assigned a range of biological properties, such as anticoagulant , antiinflammatory, antitumor and antioxidant activities. In this work, we obtained methanolic extracts from thirteen seaweeds of the coast of Rio Grande do Norte (Dictyota cervicornis; Dictiopterys delicatula; Dictyota menstruallis; D. mertensis; Sargassum filipendula; Spatoglossum schröederi; Acanthophora specifera; Botryocladia occidentalis; Caulerpa cupresoides; C. racemosa; C. prolifera; C. sertularioides e Codium isthmocladum). They were evaluated as anticoagulant and antioxidant drugs, as well as antiproliferative drugs against the tumor cell line HeLa. None of the methanolic extracts showed anticoagulant activity, but when they were evaluated as antioxidant drugs all of extracts showed antioxidant activity in all tests performed (total antioxidant capacity, sequestration of superoxide and hydroxyl radicals, ferric chelation and reductase activity), especially the algae D. mentrualis, D. cilliolata and C. prolifera, who had the greatest potential to donate electrons.In addition, the ability of iron ions chelation appears as the main antioxidant mechanism of the methanolic extracts of these seaweeds mainly for the extract of the C. racemosa seaweed, which reached almost 100% activity. In the MTT assay, all extracts showed inhibitory activity at different levels againts HeLa cells. Moreover, D. cilliolata (MEDC) and D. menstrualis (MEDM) extracts showed specific activity to this cell line, not inhibiting the viability of 3T3 normal cell line, so they were chosen for detailing the antiproliferative mechanism of action. Using flow cytometry, fluorescence microscopy and in vitro assays we demonstrated that MEDC and MEDM induced apoptosis in HeLa cells by activation of caspases 3 and 9 and yet, MEDC induces cell cycle arrest in S phase. Together, these results showed that the methanolic extracts of brown seaweed D. menstrualis and D. cilliolata may contain agents with potential use in combatting cells from human uterine adenocarcinoma. This study also points to the need for more in-depth research on phytochemical and biological context to enable the purification of biologically active products of these extracts

Host specificity of a Brazilian isolate of Alternaria cassiae (Cenargen CG593) under greenhouse conditions

Several Alternaria cassiae isolates were recovered from diseased sicklepod plants (Senna obtusifolia) in the southern regions of Brazil. A representative isolate (Cenargen CG593) was tested for its host range under greenhouse conditions. The fungus promoted symptoms in sicklepod, cassava (Manihot dulce), tomato (Lycopersicon esculentum) and eggplant (Solanum melongena) when tested at a spore concentration of 10(6) spores ml(-1). When the plants were inoculated with a suspension of 10(5) spores ml(-1) and held at a dew period of 12 h (cassava) or 18 h (tomato and eggplant), the plants showed symptoms of the disease, but they recovered and continued their normal vegetative growth. These results show that the fungus A. cassiae is safe to use for the control of S. obtusifolia under Brazilian conditions, because it did not cause excessive damage in the three plants tested.

Estudo sobre o crescimento de fedegoso

O crescimento de Senna obtusifolia foi estudado sob condições de casa-de-vegetação, num delineamento inteiramente casualizado com 4 repetições . As plantas cresceram em vasos de 6 litros preenchidos com areia e irrigados com solução completa de Hoaglan d, duas vezes por dia. O crescimento das plantas foi avaliado a partir de 21 até 161 dias após a emergência, em intervalos de 14 dias. Os resultados evidenciaram que as plantas de S. obtusifolia acumularam o máximo de matéria seca aos 147 dias após à emergência (D.A.E.) com um período de maior intensidade de crescimento compreendido entre 21 e 63 D.A. E. A partir da emergência até 63 dias, o maior acúmulo de maté ria seca foi verificado nas raízes, após esta data até o final do ciclo do fedegoso, maior proporção foi alocada nos caules. Aos 63 D.A. E. todas as plantas tinham florescido. A taxa de crescimento relativo decresceu rapidamente até 63 dias, mantendo-se baixa e constante até o final do período experimental. Este parâmetro de crescimento foi fortemente influenciado pela tal, a qual foi constante após 91 dias, provavelmente devido ao auto -sombreamento da planta . A área foliar cresceu amplamente até os 35 dias, tornando-se estável a partir dos 119 dias após a emergência das plantas. Considerando-se um período correspondente a dois terços do ciclo médio de cultura s anuais, de dez semanas, S. obtusif olia quando comparada a outras espécies daninhas, apresenta taxas de crescimento inicial menores, o que pode caracterizá-la como uma planta infestante tardia.

Períodos de interferência das plantas daninhas na cultura do algodoeiro (Gossypium hirsutum)

Com o objetivo de determinar os efeitos de períodos de controle e de convivência das plantas daninhas na produtividade da cultura do algodoeiro (Gossypium hirsutum), cultivar Delta-Opal, realizou-se um experimento que constou de dois grupos de tratamentos. No primeiro, a cultura permaneceu livre da competição das plantas daninhas desde a emergência até 7, 14, 21, 28, 35, 42, 49, 56, 63 dias e colheita (159 dias). No segundo, a cultura permaneceu em competição com a comunidade infestante desde a emergência até os mesmos períodos descritos para a primeira série de tratamentos. Dentre as espécies de plantas daninhas encontradas na área experimental, destacaram-se a tiririca (Cyperus rotundus), o fedegoso (Senna obtusifolia), a anileira (Indigofera hirsuta) e o capim-carrapicho (Cenchrus echinatus). Pelas condições edáficas, climáticas e florísticas sob as quais foi conduzida a cultura de algodão, o Período Anterior à Interferência (PAI) dessa comunidade que reduziu em 5% a produtividade da cultura foi de oito dias após a emergência da cultura (DAE); o Período Total de Prevenção da Interferência (PTPI) foi de 66 DAE; e o Período Crítico de Prevenção da Interferência (PCPI) foi dos 8 aos 66 DAE.

Controle de plantas daninhas através da palha de cana-de-açúcar associada à mistura dos herbicidas trifloxysulfuron sodium + ametrina

O objetivo deste trabalho foi avaliar os efeitos da palha de cana-de-açúcar resultante da colheita sem queima e da mistura comercial dos herbicidas trifloxysulfuron sodium + ametrina, na emergência e no acúmulo de biomassa seca de nove espécies de plantas daninhas. Os tratamentos foram distribuídos em esquema fatorial 3 x 3, sendo três quantidades de palha resultantes da colheita da cana-de-açúcar sem queima (0, 10 e 15 t ha-1), e três doses da mistura formulada dos herbicidas trifloxysulfuron sodium + ametrina (0, 1,75 e 2,00 kg ha-1), aplicados em condições de pós-emergência das plantas daninhas. As densidades das plantas daninhas foram avaliadas aos 15, 60 e 90 dias após a aplicação (DAA) da mistura dos herbicidas, e a biomassa seca das plantas daninhas, aos 90 DAA. A presença de palha sobre o solo suprimiu as densidades das populações de Brachiaria plantaginea, Digitaria horizontalis, Panicum maximum, Sida glaziovii e Amaranthus hybridus a níveis de infestação considerados satisfatórios de controle. A palhada também reduziu as populações de Senna obtusifolia, Ipomoea hederifolia, I. grandifolia e I. nil, porém em níveis insatisfatórios de controle. A mistura de herbicidas nas doses estudadas controlou todas as espécies de plantas daninhas avaliadas. Na presença da palha, o controle das plantas de S. obtusifolia, I. nil, I. hederifolia e I. grandifolia somente foi satisfatório quando foi aplicada a mistura de herbicidas. Para o controle de P. maximum, houve vantagem na integração dos dois métodos de controle, em relação à aplicação isolada da menor quantidade de palha estudada ou à aplicação da mistura de herbicidas.

Períodos de interferência das plantas daninhas na cultura da soja nos sistemas de semeadura direta e convencional

A presente pesquisa objetivou estudar os efeitos dos períodos de controle e de convivência de uma comunidade infestante sobre as características produtivas da soja nos sistemas de semeadura direta (SSD), com o cultivar CD 201, e convencional (SSC), com a MSOY-6101. No SSD, a comunidade infestante foi composta principalmente por Alternanthera tenella, Cenchrus echinatus e Bidens pilosa, com densidade máxima de 377 plantas m-2, acumulando 365,7 g m-2 de matéria seca, e, no SSC, por A. tenella, Senna obtusifolia e Panicum maximum, com densidade máxima de 161 plantas m-2, acumulando 406,7 g m-2. Tolerando cinco por cento de perda na produtividade da cultura, constatou-se que os períodos críticos de prevenção da interferência foram dos 33 aos 66 DAE para o cultivar CD 201 no SSD e dos 34 aos 76 DAE para o cultivar M-SOY-6101 no SSC. A interferência das plantas daninhas durante todo o ciclo da cultura reduziu, em média, 46% (SSD) e 32% (SSC) a produtividade de grãos da soja. Não houve efeito das plantas daninhas na estatura das plantas, na altura de inserção da primeira vagem e no número de vagens por planta.

Efeito de níveis crescentes de calagem no crescimento e estado nutricional de fedegoso

A distribuição e desenvolvimento de plantas, a exemplo daquelas que infestam as áreas agrícolas, é influenciada por diversos fatores, a como aqueles ligados às caraterísticas do solo, naturais ou impostas pelo homem. Assim, o presente trabalho de pesquisa foi desenvolvido em condições de casa-de-vegetação com o objetivo de estudar o efeito de níveis crescentes de calagem no crescimento e estado nutricional de Senna obtusifolia, planta daninha comum em ecossistemas agrícolas. O delineamento experimental adotado foi o inteiramente casualizado, com seis tratamentos e quatro repetições. Os tratamentos constituíram-se em quantidades de calcário dolomítico calcinado com 38% de CaO e 20% de MgO, com um poder de neutralização total (PRNT) de 104%: 0, 2, 4, 6, 8 e 10 t/ha. As parcelas experimentais constituíram-se de recipientes de plástico com capacidade de 5 litros, preenchidos com terra coletada na camada arável de um latossolo vermelho escuro distrófico, A moderado, textura média. Após um período de crescimento de 56 dias as plantas foram coletadas e separadas em seus componentes vegetativos, sendo colocados a secar em estufa de circulação forçada de ar (70ºC por 72 horas) para determinação do peso seco. O material foi pesado e moído e analisado a nível de composição de nutrientes. Os resultados mostraram efeitos negativos dos níveis crescentes de calcário, sobre o crescimento das plantas de S. obtusifolia, evidenciando-se decréscimos nos valores da matéria seca acumulada a partir da dose de 4 t/ha de calcário (pH 5,8), com efeitos mais marcantes nas raízes. A aplicação de doses crescentes de calcário promoveu um aumento progressivo nos teores de Ca e Mg no solo, o que talvez possa ter estimulado a competição entre cátions, reduzindo a absorção do K. Os elementos N e P foram os mais absorvidos pela planta.

Diálogos com o homem imaginário: pensando o uso de imagens no ensino de sociologia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Lévi-Strauss Mitológicas: Mito e música entre o largo e o prestíssimo

This study was elaborated based on our research of the work Mithologiques by the anthropologist Claude Lévi-Strauss (1908-2009), which affirms that languages, indigenous myths and music are related. He proposes that the understanding of myths occurs in a similar manner as with an orchestral score. In the course of his tetralogy we investigated the musical terms used in the analysis and in the division of the chapters, especially in the first volume of his work. Several compositional procedures and forms are named. Composers in pairs are categorized: Sebastian Bach for the code, Ludwig van Beethoven for the message, and Richard Wagner for the myths. In this deduction, we structured in parts: theme and variations, sonata and fugue with the aforementioned composers. Within the greatness of anthropological study, from among over 800 myths, we selected the first five of the indigenous tribe Bororo to discuss within the Theme and Variation segment. In the Sonata part there are two myths with the same theme: The wife of the jaguar which relates to the compositional structure, and four myths about The origin of women. Finally, in the segment related to the Fugue, we collected four myths that address The shortness of life. Honoring the many terms expressed in opposition, contrast, or symmetry under consideration in Levi-Strauss work, we entitled this thesis emphasizing the migration between the tempos Largo and Prestíssimo as these are oppositional presentations in music. Fifteen musical myths accompany the work supported by selected narratives. In light of this we questioned, we questioned: how are incest, murder and other events part of a society that elevates nature as an extension of life itself? And how did Lévi-Strauss think that anthropology harmonized with music? In the preparation of this study, philosophers like Peter Sloterdijk discuss the circular territory of Mythology

A (re)significação conceptual nas práticas colaborativas com professores do Ensino Fundamental

The work objectified to apprehend the degree of the teachers' concept concerning the territory concept and to intervene with situations of critical reflections to accompany and to analyze the process of conceptual elaboration. It contemplates on the (new)meaning of knowledge and (new)elaboration of the concept in study done inside a pedagogic intervention. The Municipal School Dr. Julio Senna - Ceará-Mirim/RN and six (6) teacher-collaborators that taught in the 3rd and 4th grades of the fundamental teaching, constitutes the empiric field of the research. Its theoretical-methodological contributions are built in the studies of Vigotski (2000a, 2000b and 2001) on the formation process and development of concepts; in the methodology colaborate (Ibiapina (2004), Bartomé (1986), Kemmis and Mctaggart (1988), Arnal, Del Ricon and Latorre (1992), Pepper and Ghedin (2002), among others) and in the critical-reflexive conception of the Geography (Soares Júnior (2000 and 1994), Silva (1998), Raffestin (1993), Santos (1994), Felipe (1998), among others). The accomplishment of the work presupposed starting from the reflections on the following subjects: which the teachers' understanding in the school space concerning the territory concept? How does happen the process of conceptual construction territory for the teachers? The analysis of the teachers' previous knowledge on the concept in study, evidenced that its apprehensions on the attributes of the referred concept went mentioned the to light of the perceptible dimension of the real-concrete relationships of the reality linked to the degree of the spontaneous concepts and followed by the ideas of the traditional, humanistic and cultural geographical conceptions (positivism and phenomenology), restricting the territory meaning the notion of State-Nation and place of the men's dwelling. In the intervention process, it was verified to real possibility of the acquisition of indispensable scientific concepts to the process of (new)meaning conceptual of geographical knowledge through the continuous practice of the educational formation, when it was evidenced that the teacher-collaborators acquired high degrees of attributions of the significance of the territory concept to the they elaborate generalizations by means of analyses and syntheses of the concept-attribute (essential and multiples) of the reference conceptual in study

Dispersion of Seeds of Tree Species by the Leaf-Cutting Ant Acromyrmex subterraneus molestans (Hymenoptera: Formicidae) in Vicosa, Minas Gerais State, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A implementação do programa Gestão nota 10 no sistema municipal de educação em Mossoró/RN (2005 a 2009)

This research work is focused to show the changes in educational administration from the agreements between the Mossoró / RN and the Ayrton Senna Institute IAS, for education provision. Nowadays, the partnership policy is a constitutive element of the reform of the Brazilian State, which dropped its action on social policies and to strengthen its regulatory role, encouraging private participation in planning, preparation and implementation of public policies, new printing setting the political-social. In this context, the 10 Note Management Programme, developed by the IAS, is part of the neoliberal logic of modernization of public school systems, focusing on results and developing strategies for control and regulation of schools work and its efficiency, effectiveness and greater productivity. The 10 Note focuses on two dimensions: the management of learning and teaching in networking, in a managerial perspective to overcome the culture of failure (expressed as age-grade, dropout and repetition rates in) and implantation of culture of success (as measured in the improvement of the indices). To understanding the process, we have delimited as the object of study, the process of implementing them mentioned program in the city, which its objective is to analyze implications for the school community from the perspective of democratic management, adopting the dimensions of autonomy and participation in institutional processes as a criterion of analysis. From a methodological point of view, the survey was conducted from a literature review and documentary about educational policy developed in the country since the 1990´s, seeking to understand, in a dialectical perspective, the political dimensions of teaching, training and performance of the subjects involved in the school work. Besides the empirical observation, it was also used semi-structured interviews with a methodological tool for gathering information and opinions about the partnership and the implementation of the 10 Note Management Program in the county. The interviewee participants were ex-former education managers, coordinators, school managers, secretaries and teachers. Regarding the dimensions inside the analysis (autonomy and participation), the research led to the conclusion: that GEED, under the guidance of IAS promoted regulation of school autonomy, set up the selection process for exercising the office of school administration and system awards to schools, pupils and teachers, subject to results, there is mismatch between the managerial logic and the democratic management principles, that the ideological discourse of modernization of municipal management coexists with traditional practices, centralizing patronage, which ignores the democratic participation in the school decisions processes, the goals of the partnership were partially achieved, since that the city has improved over the approval and dropouts, although the approval of the Education Municipal Plan of the rules institutional (administrative, financial and educational) and the creation of the Councils observed that the school community participation is still limited, not being characterized as a coordinated intervention, capable of promoting the transformation and improvement its quality in the county. In the same way, the orientation of networking is a limit to the autonomy of schools, given the external definition of goals and strategies to be adopted, along with pressure exerted through the accountability of each school community for their achievements

Frequência de Lettuce big-vein associated virus e Mirafiori lettuce big-vein virus em Plantas de alface no Estado de São Paulo e transmissão através de extrato vegetal e solo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The pulvinus endodermal cells and their relation to leaf movement in legumes of the Brazilian cerrado

Legume pulvini have a clearly delimited endodermis, whose variable content has been associated with the velocity and type of leaf movement: pulvini in leaves with fast nastic movement contain starch grains; pulvini in leaves with slow nastic movements have calcium oxalate crystals as well as starch grains in the endodermis. However, the studies carried out to date have involved few legume species. This study therefore purported to examine the consistency of this hypothesis in other legumes. Thus, the structure and content of the pulvinus endodermal cells of nine legumes of the Brazilian cerrado, with different types and velocities of leaf movement, were investigated: slow nyctinastic and heliotropic movements (Bauhinia rufa, Copaifera langsdorffii, Senna rugosa - Caesalpinioideae; Andira humilis and Dalbergia miscolobium - Faboideae; Stryphnodendron polyphyllum - Mimosoideae), slow heliotropic movement (Zornia diphylla - Faboideae), and fast seismonastic and slow nyctinastic and heliotropic movements (Mimosa rixosa and Mimosa flexuosa - Mimosoideae). Samples were prepared following standard plant anatomy and ultrastructure techniques. The endodermis of all the species contains starch grains. In the species displaying only slow movements, calcium oxalate prismatic crystals were observed in addition to starch grains, except in Zornia diphylla. in conclusion, oxalate crystals occur only in endodermal cells of pulvini that display slow movements, while starch grains are always present in pulvinus endodermal cells of plants with any kind of movement.

THE EXTENT of SEED PREDATION BY BRUCHINE BEETLES (COLEOPTERA: CHRYSOMELIDAE: BRUCHINAE) IN A HETEROGENEOUS LANDSCAPE IN SOUTHEASTERN BRAZIL

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)