Remodeling of the Actin Cytoskeleton Is Coordinately Regulated by Protein Kinase C and the ADP-Ribosylation Factor Nucleotide Exchange Factor ARNO

ARNO is a member of a family of guanine-nucleotide exchange factors with specificity for the ADP-ribosylation factor (ARF) GTPases. ARNO possesses a central catalytic domain with homology to yeast Sec7p and an adjacent C-terminal pleckstrin homology (PH) domain. We have previously shown that ARNO localizes to the plasma membrane in vivo and efficiently catalyzes ARF6 nucleotide exchange in vitro. In addition to a role in endocytosis, ARF6 has also been shown to regulate assembly of the actin cytoskeleton. To determine whether ARNO is an upstream regulator of ARF6 in vivo, we examined the distribution of actin in HeLa cells overexpressing ARNO. We found that, while expression of ARNO leads to disassembly of actin stress fibers, it does not result in obvious changes in cell morphology. However, treatment of ARNO transfectants with the PKC agonist phorbol 12-myristate 13-acetate results in the dramatic redistribution of ARNO, ARF6, and actin into membrane protrusions resembling lamellipodia. This process requires ARF activation, as actin rearrangement does not occur in cells expressing a catalytically inactive ARNO mutant. PKC phosphorylates ARNO at a site immediately C-terminal to its PH domain. However, mutation of this site had no effect on the ability of ARNO to regulate actin rearrangement, suggesting that phosphorylation of ARNO by PKC does not positively regulate its activity. Finally, we demonstrate that an ARNO mutant lacking the C-terminal PH domain no longer mediates cytoskeletal reorganization, indicating a role for this domain in appropriate membrane localization. Taken together, these data suggest that ARNO represents an important link between cell surface receptors, ARF6, and the actin cytoskeleton.

The mineral composition of some sands from Quebec, Labrador and Greenland / by James H.C. Martens. Results of the Rawson-MacMillan Sub-Arctic Expedition of 1926. Oliver Cummings Farrington -- editor.

v.5:no.2(1929)

Famous diamonds/ by Oliver C. Farrington, Curator of Geology.

no.10(1929)

Amber: its physical properties and geological occurrence / by Oliver C. Farrington, Curator of Geology.

no.3(1923)

Meteorites / by Oliver C. Farrington, Curator of Geology.

no.4(1923)

Battlefield of Spotsylvania Court House, Virginia and vicinity, 1864 (Raster Image)

This layer is a georeferenced raster image of the historic paper map: Map of the battle field of Spottsylvania C.H. : showing the field of operations of the Army of the Potomac commanded by Maj. Gen. George G. Meade U.S.A., from May 8th to 21st, 1865 [i.e. 1864], surveyed under the orders of Bvt. Col. J.C. Duane, Major of Engineers, Chief Engineer, Army of the Potomac, by Bvt. Maj. C.W. Howell, 1st Lieut. of Engineers ; assisted by Messrs. L.C. Oswell, L. Bell, and R.B. Talfor ; J. Bien, lithographer, New York. It was published ca. 1865. Scale [1:15,840]. Covers area surrounding Spotsylvania and Spotsylvania Battlefield, Virginia. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Virginia State Plane North Coordinate System (in Meters) (Fipszone 4501). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, drainage, dwellings with names of inhabitants, vegetation, Union and Confederate troop lines and defenses, and more. Relief shown by hachures. Includes note. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Martinsburg, West Virginia and vicinity, ca. 1864 (Raster Image)

This layer is a georeferenced raster image of the historic paper manuscript map entitled: Plan of Martinsburg and vicinity. "This map was drawn by that noble officer Capt. C.L. Chandler and was much prized by our late Col. Geo. D. Wells, Chas. H. Howland, 1st Lt. & B. Gen., 34th Mass. Inf."-verso. It was sketched ca. 1864. Scale [ca. 1:9,250]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the West Virginia State Plane North Coordinate System (in Meters) (Fipszone 4701). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, railroads, selected dwellings with names of inhabitants, lines of pickets, troop location and camps, drainage, and more. Relief shown by form lines. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

The law of charitable uses : argument of Wm. Curtis Noyes, before the Court of Appeals of the State of New York, in the case of Beekman, administrator, &c., against the people, &c., and others, in behalf of Thomas Beekman, one of the respondents, with an appendix containing the will.

W. Barthrop's will and codicils: p. 93-102.

The complete confectioner, or, Housekeeper's guide to a simple and speedy method of understanding the whole art of confectionary : the various ways of preserving and candying, dry and liquid, all kinds of fruit, nuts, flowers, herbs, &c. And the method of keeping them fresh and fine all the year round; the different ways of clarifying sugar; with directions for making fruit pastes, bomboons, pastils, compotes, fruit ices, cream ices, marmalades, jellies, jams, cakes, puffs, biscuits, tarts, custards, cheesecakes, sweetmeats, fritters, creams, syllabubs, blanc-mange, flummeries, ornaments for grand entertainments, dragees, syrups of all kinds, nicknacks and trifles for desserts, strong cordials, oils, simple waters, milk punch that will keep 20 years, and all sorts of English wines. Also, the art of making artificial fruit, with the stalks in it, so as to resemble the natural fruit. To which are added, some bills of fare for desserts for private families /

Originally published under title: The compleat confectioner. Cf. Bitting, K.G. Gastronomic bib., p. 190.

The young woman's companion, or, Frugal housewife : containing the most approved methods of pickling, preserving, potting, collaring, confectionary, managing and colouring foreign wines and spirits, making English wines, compounds, &c., &c. : also, the art of cookery containing directions for dressing all kinds of butchers' meat, poultry, game, fish, &c., &c. : with the complete art of carving, illustrated and made plain by engravings : likewise instructions for marketing : with the theory of brewing malt liquor : to which are added directions for letter writing, drawing, painting, &c., and several valuable miscellaneous pieces.

Includes index

The complete economical cook and frugal housewife : an entirely new system of domestic cookery : containing approved directions for purchasing, preserving, and cooking : also, trussing and carving, preparing soups, gravies, sauces, made dishes, potting, pickling, &c. : with directions for pastry and confectionery : likewise the art of making British wines, brewing, baking, &c. /

"Bills of fare": p. 63-90; "Table of income and wages": p. 426; "Marketing tables": p. [427]-432.

Every-day cookery for every family : containing nearly 1,000 receipts adapted to moderate incomes and comprising the best and most economical methods of roasting, boiling, broiling, and stewing all kinds of meats, fish, poultry, game and vegetables : simple inexpensive instructions for making pies, puddings, tarts and all other pastry : how to pickle and preserve fruits and vegetables : suitable cookery for invalids and children : food in season and how to choose it : the best ways to make domestic wines and syrups : and ample receipts for bread, cake, soups, gravies, sauces, desserts, jellies, brandied fruits, soaps, perfumes, etc., etc. : and ample directions for carving /

"Domestic wines and liquors": p. 226-236.

The semi-centennial catalogue of Davidson College, Davidson, N.C., 1837-1887 /

Includes index.