Santa Barbara county units of study for teachers in elementary schools ...

v. 5. Santa Barbara county guide for teachers of children of differnt cultures.

Improvisation 27 (Garden of Love II)

Vassily Kandinsky; 3 ft. 11 23/64 in.x 4 ft. 7 15/64 in.; oil on canvas

Miss Tiller's vegetable garden and the money she made by it.

Mode of access: Internet.

Native Hawaiian Health Care Improvement Act : hearing before the Committee on Indian Affairs, United States Senate, One Hundred Sixth Congress, second session, on S. 1929, to amend the Native Hawaiian Health Care Improvement Act to revise and extend such act.

Shipping list no.: 2000-0248-P (pt. 1), 2000-0250-P (pt. 2), 2000-0290-P (pt. 3), 2000-0308-P (pt. 4).

The story of California ; Gold ; The gray dawn ; The rose dawn /

Mode of access: Internet.

Controlling protein molecular dynamics:how to accelerate folding while preserving the native state

The dynamics of peptides and proteins generated by classical molecular dynamics (MD) is described by using a Markov model. The model is built by clustering the trajectory into conformational states and estimating transition probabilities between the states. Assuming that it is possible to influence the dynamics of the system by varying simulation parameters, we show how to use the Markov model to determine the parameter values that preserve the folded state of the protein and at the same time, reduce the folding time in the simulation. We investigate this by applying the method to two systems. The first system is an imaginary peptide described by given transition probabilities with a total folding time of 1 micros. We find that only small changes in the transition probabilities are needed to accelerate (or decelerate) the folding. This implies that folding times for slowly folding peptides and proteins calculated using MD cannot be meaningfully compared to experimental results. The second system is a four residue peptide valine-proline-alanine-leucine in water. We control the dynamics of the transitions by varying the temperature and the atom masses. The simulation results show that it is possible to find the combinations of parameter values that accelerate the dynamics and at the same time preserve the native state of the peptide. A method for accelerating larger systems without performing simulations for the whole folding process is outlined.

United States Botanic Garden, National Garden.

Shows individual plantings and pathways.

Visitor guide, United States Botanic Garden.

"4/08."

Visitor guide, United States Botanic Garden.

"8/08."

Visitor guide, United States Botanic Garden.

"10/2011."

Estudio de factibilidad técnico-económico para el cultivo de peces de tilapia en jaulas flotantes aplicado al rancho Santa Lucía del cantón quitasol, caserio el coyolito K-481/2 Tejutla, Chalatenango.

Este documento está diseñado principalmente para el Consejo Nacional de Empresarios Salvadoreños (CONAES) y para el propietario del Rancho Santa Lucia, su propósito fue el preparar un estudio de factibilidad técnico-económico para el cultivo de peces tilapia en jaulas flotantes, el cual incluye en su contenido métodos como el analítico, sintético y descriptivo para realizar un adecuado diagnóstico de la problemática actual y obtener una perspectiva más precisa de la magnitud del problema. También se establecieron las fuentes de información primarias a través de las técnicas como la entrevista, la encuesta y la observación directa, además de otro tipo de información para el marco teórico en el que se fundamenta la investigación, entre estos se incluyen libros, revistas, informes, periódicos, sitios Web, entre otras fuentes secundarias. La población objeto de análisis se dividió en dos universos: el primero constituido por 27 consumidores que incluyen restaurantes, merenderos y comerciantes de productos pesqueros de los mercados de Nueva Concepción, La Reina, Chalatenango y caserío El Coyolito, y el segundo conformado por una asociación que cuenta con 7 cooperativas afiliadas ubicadas en los alrededores del Embalse del Cerrón Grande y tres peceras privadas ubicadas en los municipios de Santa Bárbara y Nueva Concepción; el instrumento de recolección de datos fue el cuestionario, el cual permitió llevar a cabo el censo de dicho universo. Los resultados obtenidos llevan a una conclusión principal, la cual indica que existe potencial de demanda para los peces tilapia, y de esta se deriva la recomendación que sugiere invertir en este proyecto, debido a que actualmente la demanda de peces tilapia no esta siendo cubierta. De los resultados del diagnóstico se efectuó la propuesta de inversión, que hace referencia a las variables principales de mercado, técnicas, organizacionales, legales, económicas y financieras que demandará y se generará con el proyecto.

Phosphodiesterase 6 generates intracellular cGMP microdomains in the native endothelium

Endothelial cells (EC) are essential regulator of vascular homeostasis through the generation and release of various bioactive agents, including nitric oxide (NO). NO modulates several vascular functions such as vascular tone and permeability, through the stimulation of soluble guanylate cyclase (sGC) leading to the production of cGMP. Conversely, phosphodiesterases (PDEs) are enzymes metabolizing cyclic nucleotides (cGMP and cAMP) and are therefore major regulatory players for cGMP and cAMP signalling pathways. Although ECs are the main source of NO, little is known on the endothelial NO-cGMP signalling pathway and cellular outcomes. It was then hypothesized that a specific population of cGMP-phosphodiesterases allows ECs to stabilize cGMP levels despite the elevated production of NO. Expression of cGMP-phosphodiesterases was initially studied in resistance mesenteric arteries from mice. PDE5 and PDE6 were both found at mRNA and protein levels in native arteries but PDE6 is not found in cultured ECs. Interestingly, subcellular distributions of both enzymes were distinct. PDE5 appeared to be homogeneously distributed whilst PDE6 catalytic subunits (PDE6 and PDE6) showed a preferential staining in the perinuclear region. These results suggest that PDE6 might be involved in the regulation of cGMP microdomains. Based on these findings, a mathematical model was developed. Simulations of dynamic cGMP levels in ECs support the notion of cGMP microdomains dependent on PDE6 expression and localization. In the absence of PDE6, application of NO either as a single bolus or repetitive pulses led to a homogeneous increase in cGMP levels in ECs despite PDE5 homogeneous distribution. However, PDE6 subcellular targeting to the perinuclear membrane generated a cGMP-depleted perinuclear space. The findings from this study provide the first evidence of the expression and specific intracellular distribution of PDE6 in native endothelial cells that strongly support their involvement in the generation of cGMP microdomains

Phosphodiesterase 6 generates intracellular cGMP microdomains in the native endothelium

Endothelial cells (EC) are essential regulator of vascular homeostasis through the generation and release of various bioactive agents, including nitric oxide (NO). NO modulates several vascular functions such as vascular tone and permeability, through the stimulation of soluble guanylate cyclase (sGC) leading to the production of cGMP. Conversely, phosphodiesterases (PDEs) are enzymes metabolizing cyclic nucleotides (cGMP and cAMP) and are therefore major regulatory players for cGMP and cAMP signalling pathways. Although ECs are the main source of NO, little is known on the endothelial NO-cGMP signalling pathway and cellular outcomes. It was then hypothesized that a specific population of cGMP-phosphodiesterases allows ECs to stabilize cGMP levels despite the elevated production of NO. Expression of cGMP-phosphodiesterases was initially studied in resistance mesenteric arteries from mice. PDE5 and PDE6 were both found at mRNA and protein levels in native arteries but PDE6 is not found in cultured ECs. Interestingly, subcellular distributions of both enzymes were distinct. PDE5 appeared to be homogeneously distributed whilst PDE6 catalytic subunits (PDE6 and PDE6) showed a preferential staining in the perinuclear region. These results suggest that PDE6 might be involved in the regulation of cGMP microdomains. Based on these findings, a mathematical model was developed. Simulations of dynamic cGMP levels in ECs support the notion of cGMP microdomains dependent on PDE6 expression and localization. In the absence of PDE6, application of NO either as a single bolus or repetitive pulses led to a homogeneous increase in cGMP levels in ECs despite PDE5 homogeneous distribution. However, PDE6 subcellular targeting to the perinuclear membrane generated a cGMP-depleted perinuclear space. The findings from this study provide the first evidence of the expression and specific intracellular distribution of PDE6 in native endothelial cells that strongly support their involvement in the generation of cGMP microdomains