In vivo and in vitro effects of RAD001 on bladder cancer

Objective: To evaluate the influence of Everolimus (RAD001) on chemically induced urothelial lesions in mice and its influence on in vitro human bladder cancer cell lines. Methods: ICR male mice were given N-butyl-N-(4-hydroxybutyl) nitrosamine in drinking water for a period of 12 weeks. Subsequently, RAD001 was administered via oral gavage, for 6 weeks. At the end of the experiment, all the animals were sacrificed and tumor development was determined by means of histopathologic evaluation; mammalian target of rapamycin (mTOR) expressivity was evaluated by immunohistochemistry. Three human bladder cancer cell lines (T24, HT1376, and 5637) were treated using a range of RAD001 concentrations. MTT assay, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and flow cytometry were used to assess cell proliferation, apoptosis index, and cell cycle analysis, respectively. Immunoblotting analysis of 3 cell line extracts using mTOR and Akt antibodies was performed in order to study the expression of Akt and mTOR proteins and their phosphorylated forms. Results: The incidence of urothelial lesions in animals treated with RAD001 was similar to those animals not treated. RAD001 did not block T24 and HT1376 cell proliferation or induce apoptosis. A reduction in cell proliferation rate and therefore G0/G1 phase arrest, as well as a statistically significant induction of apoptosis (P 0.001), was only observed in the 5637 cell line. Conclusion: RAD001 seems not to have a significant effect on chemically induced murine bladder tumors. The effect of RAD001 on tumor proliferation and apoptosis was achieved only in superficial derived bladder cancer cell line, no effect was observed in invasive cell lines.

Rupicapra rupicapra (Mammalia) in the Late Pleistocene of Portugal

The presence of the genus Rupicapra in Portugal is shown for the first time, on some dental and skeletal remains from the upper Pleistocene (Solutrean) of Salemas cave. The fossil material may be ascribed to R. rupicapra pyrenaica.

Paleolítico médio e superior em Portugal: datas 14C, estado actual dos conhecimentos,síntese e duscussão

New radiocarbon measurements were obtained from middle and upper Paleolithic sites currently under research by the CEPUNL, in well defined stratigraphical situations. With other dates, they yield an approximative chronological global view. Measurement distribution in function of time does not seem to be an hazardous one. In the actual status of our knowledge, this distribution seems to fit in some assemblages: (a) 14000 to 15000 BP, Solutrean; (b) about 20 000 BP, Solutrean; (c) circa 25 000 BP, already Solutrean, and slightly older than 26 000 BP, still Mousterian; (d) between 29 000 and 31 000 BP, Mousterian. The persistance of Mousterian much later than its acknowledged upper limit at about 34 000 BP (and hence the survival of its neanderthalian authors) is demonstrated. For the first time it has been possible to ascertain the upper time limit of the marine 5-8 metres raised beach (Tyrrhenian III) at Serra da Arrábida, and also the age of archaeological sites without stone artifacts, or with uncharacteristic ones. The obtained measurements allow US to correlate localities and sedimentary units with last glaciation events. There seems to have been a distinct correlation between cave and shelter human occupation and events marked by the worsening of climate.

Efeito de filtros ópticos na função visual de espeleologistas

Speleologist’s perform their activity in demanding visual conditions of very low luminance - many visual tasks involve resolution of detail under conditions of low contrast. Work related conditions in a cave as exposure to heat, chemicals, dust and poor lighting conditions could influence the integrity of the visual system and predispose the eye to diseases that eventually affect vision. Poor lighting conditions cause a variety of symptoms of visual discomfort and may increase the risk of accidents. Good visual acuity is crucial for several and has an important role for safety purposes. The aim of this study was to evaluate lighting conditions and optical filters effects on visual performance in speleologists exposed to cave environments.

Gruta da figueira brava (Arrabida): Geological setting

Separata do Tomo XXXVIII das Memories da Academia das Ciencias de Lisboa (Classe de Ciencias)

Remote experimentation network - yielding an inter-university peer-to-peer e-service

The goal of this paper is to discuss the benefits and challenges of yielding an inter-continental network of remote laboratories supported and used by both European and Latin American Institutions of Higher Education. Since remote experimentation, understood as the ability to carry out real-world experiments through a simple Web browser, is already a proven solution for the educational community as a supplement to on-site practical lab work (and in some cases, namely for distance learning courses, a replacement to that work), the purpose is not to discuss its technical, pedagogical, or economical strengths, but rather to raise and try to answer some questions about the underlying benefits and challenges of establishing a peer-to-peer network of remote labs. Ultimately, we regard such a network as a constructive mechanism to help students gain the working and social skills often valued by multinational/global companies, while also providing awareness of local cultural aspects.

Collaborative learning in a qeb-accessible workbench

Web-based course management and delivery is regarded by many institutions as a key factor in an increasingly competitive education and training world, but the systems currently available are largely unsatisfactory in terms of supporting collaborative work and access to practical science facilities. These limitations are less important in areas where “pen-and-paper” courseware is the mainstream, but become unacceptably restrictive when student assignments require real-time teamwork and access to laboratory equipment. This paper presents a web-accessible workbench for electronics design and test, which was developed in the scope of an European IST project entitled PEARL, with the aim of supporting two main features: full web access and collaborative learning facilities.

Institutional factors governing the deployment of remote experiments: lessons from the rexnet project

Remote labs offer many unique advantages to students as they provide opportunities to access experiments and learning scenarios that would be otherwise unavailable. At the same time, however, these opportunities introduce real challenges to the institutions hosting the remote labs. This paper draws on the experiences of the REXNET project consortium to expose a number of these issues as a means of furthering the debate on the value of remote labs and the best practices in deploying them. The paper presents a brief outline of the various types of remote lab scenarios that might be deployed. It then describes the key human and technological actors that have an interest in or are intrinsic to a remote lab instance, with a description of the role of each actor and their interest. Some relationships between these various actors are then discussed with some factors that might influence those relationships. Finally some general issues are briefly described.

Rupicapra rupicapra (Mammalia) In the Late Pleistocene of Portugal

The presence of the genus Rupicapra in Portugal is shown for the first time, on some dental and skeletal remains from the upper Pleistocene (Solutrean) of Salemas cave. The fossil material may be ascribed to R. rupicapra pyrenaica.

Paleolítico médio e superior em Portugal: datas 14C, estado actual dos conhecimentos, síntese e discussão

New radiocarbon measurements were obtained from middle and upper Paleolithic sites currendy under research by the CEPUNL, in well defined stratigraphical situations. With other dates, they yield an approximative chronological global view. Measurement dístribution in function of time does not seem to be an hazardous one. ln the actual status of our knowledge, this distribution seems to fit in some assemblages: (a) 14000 to 15000 BP, Solurrean ; (b) about 20 000 BP, Solurrean ; (e) area 25 000 BP, already Solurrean, and slightly older than 26 000 BP, still Mousterian ; (d) between 29 000 and 31 000 BP, Mousterian. The persistance of Mousterian much later than its acknowledged upper limit at about 34 000 BP (and hence the survival of its neanderthalian authors) is demonstrated. For the first time it has been possible to ascertain the upper time limit of the marine 5-8 metres raised beach (Tyrrhenian III) at Serra da Arrábida, and also the age of archaeological sires wirhout srone arrifacts, or with uncharacteristic ones. The obtained measurements allow us to correlate localities and sedimentary units with last glaciation events. There seems to have been a distinct correlation between cave and shelter human occupation and events marked by the worsening of climate.

Paleolítico médio em Galapos (Arrábida)

Quartz (and silex) lithic artifacts collected at Galapos (Creiro), in the southern part of lhe Serra da Arrábida, Portugal, are described. These artifacts are characteristic of the Middle Paleolithic (Mousterian). This new locality reinforces lhe evidences for an important human occupation of this region at those times, as previously shown by the study of the Figueira Brava cave.

Quaternary elephants in Portugal: new data

In this paper, a synopsis on the already known data, and the study of further specimens either new (*) or hither to undescribed are presented. The specimens dealt with have been collected at the Condeixa travertines; in the Tagus terraces near Carregado(*) and at Foz do Enxarrique(*); in the mousterian, about 31000 BP infilling from the Figueira Brava cave(*); and at Algar de Joao Ramos(*). Some specimens are ascribed to Elephas antiquus, the best represented form. Material from Figueira Brava cave and Algar de João Ramos is ascribed to Mammuthus primigenius, a species recognized here for the first time in Portugal.

Random amplified polymorphic DNA (RAPD) analysis of Lutzomyia longipalpis laboratory populations

The phlebotomine sand fly Lutzomyia longipalpis has been incriminated as a vector of American visceral leishmaniasis, caused by Leishmania chagasi. However, some evidence has been accumulated suggesting that it may exist in nature not as a single but as a species complex. Our goal was to compare four laboratory reference populations of L. longipalpis from distinct geographic regions at the molecular level by RAPD-PCR. We screened genomic DNA for polymorphic sites by PCR amplification with decamer single primers of arbitrary nucleotide sequences. One primer distinguished one population (Marajó Island, Pará State, Brazil) from the other three (Lapinha Cave, Minas Gerais State, Brazil; Melgar, Tolima Department, Colombia and Liberia, Guanacaste Province, Costa Rica). The population-specific and the conserved RAPD-PCR amplified fragments were cloned and shown to differ only in number of internal repeats.

Análise técnico-económica dos diferentes tipos de tubagens e acessórios

Dissertação para obtenção do grau de Mestre em Engenharia Civil – Perfil de Construção

The genetic basis of morphological change in convergent evolution of natural populations: identifying candidate genes behind convergent evolution in blind cavefish astyanax mexicanus

Dissertation presented to obtain the Ph.D. degree in Biology at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa.