Stellwagen Bank National Marine Sanctuary final environmental impact statement/management plan / Sanctuaries and Reserves Division.

1

Annual report of the Marine Mammal Commission : a report to Congress.

1995

Annual report of the Marine Mammal Commission : a report to Congress.

1989

Stellwagen Bank National Marine Sanctuary final environmental impact statement/management plan / Sanctuaries and Reserves Division.

2

Report of Lepocreadium Bimarium manter, 1940, Vitellibaculum Spinosa (Siddioi) & Cable, 1960) and Hirudinella Ventricosa (Pallas, 1774), parasites of marine fishes in Brazil

Lepocreadium bimarinum and Vitellibaculum spinosa are referred for the first time in Stephanolepis hispidus and in Brazil, and Hirudinella ventricosa is reported from Scomberomorus cavalla. Measurements, figures and comments are given.

Dinosoma clupeola sp. n. (Hemiuridae) and Pseudoacanthostomum floridensis Nahhas & Short, 1965 (Acanthostomidae), digenetic trematodes in Brazilian Marine fishes

Dinosoma clupeola sp. n. is described from Harengula clupeola, and resembles D. hawaiiense Yamaguti, 1970, from which it differs in the entire, elongate-saccular seminal vesicle, tegument weakly plicated, smaller size of body and internal organs, and slightly larger and narrower eggs. Pseudoacanthostomum floridensis Nahhas & Short, 1965 is referred to Netuma barba, which represents a new host record.

Schikhobalotrema solitaria sp. n. and S. acanthuri Yamaguti, 1970 (Haplosplanchnidae: Digenea) in Brazilian marine fishes

Schikhobalotrema solitaria sp. n. is described from Stephanolepis hispidus (Linnaeus, 1758), and is characterized by the relation between oral sucker and pharynx 1:1,1. This Relation in the other species ranges between 1:0.3-0.6. It is more similar to S. manteri Siddiqi & Cable, 1960 in the distribution of vitellaria, differing from it in the size of vitelline follicles, extension of uterus and in the size of the eggs. S. acanthuri Yamaguti, 1970 is referred for the first time in South America in Mugil liza Valenciennes, 1836 representing a new host record.

Marine biotoxins in the Catalan littoral: could biosensors be integrated into monitoring programmes?

Aquest article descriu els sensors enzimàtics i immunosensors electroquímics que s’han desenvolupat als nostres grups per a la detecció de la biotoxina marina àcid okadaic (OA), i discuteix la possibilitat d’integrar-los en programes de seguiment. Els sensors enzimàtics per a OA que es presenten es basen en la inhibició de la proteïna fosfatasa (PP2A) per aquesta toxina i la mesura electroquímica de l’activitat enzimàtica mitjançant l’ús de substrats enzimàtics apropiats, electroquímicament actius després de la seva desfosforació per l’enzim. Els immunosensors electroquímics descrits en aquest article es basen en un enzimoimmunoassaig sobre fase sòlida competitiu indirecte (ciELISA), amb fosfatasa alcalina (ALP) o peroxidasa (HRP) com a marcatges, i un sistema de reciclatge enzimàtic amb diaforasa (DI). Els biosensors presentats aquí s’han aplicat a l’anàlisi de dinoflagel·lats, musclos i ostres. Les validacions preliminars amb assaigs colorimètrics i LC-MS/MS han demostrat la possibilitat d’utilitzar les bioeines desenvolupades per al cribratge preliminar de biotoxines marines en mostres de camp o de cultiu, que ofereixen informació complementària a la cromatografia. En conclusió, tot i que encara cal optimitzar alguns paràmetres experimentals, la integració dels biosensors a programes de seguiment és viable i podria proporcionar avantatges respecte a altres tècniques analítiques pel que fa al temps d’anàlisi, la simplicitat, la selectivitat, la sensibilitat, el fet de poder ser d’un sol ús i l’efectivitat de cost. This article describes the electrochemical enzyme sensors and immunosensors that have been developed by our groups for the detection of marine biotoxin okadaic acid (OA), and discusses the possibility of integrating them into monitoring programmes. The enzyme sensors for OA reported herein are based on the inhibition of immobilised protein phosphatase 2A (PP2A) by this toxin and the electrochemical measurement of the enzyme activity through the use of appropriate enzyme substrates, which are electrochemically active after dephosphorylation by the enzyme. The electrochemical immunosensors described in this article are based on a competitive indirect Enzyme- Linked ImmunoSorbent Assay (ciELISA), using alkaline phosphatase (ALP) or horseradish peroxidase (HRP) as labels, and an enzymatic recycling system with diaphorase (DI). The biosensors presented herein have been applied to the analysis of dinoflagellates, mussels and oysters. Preliminary validations with colorimetric assays and LC-MS/MS have demonstrated the possibility of using the developed biotools for the preliminary screening of marine biotoxins in field or cultured samples, offering complementary information to chromatography. In conclusion, although optimisation of some experimental parameters is still required, the integration of biosensors into monitoring programmes is viable and may provide advantages over other analytical techniques in terms of analysis time, simplicity, selectivity, sensitivity, disposability of electrodes and cost effectiveness.

Some monogenoidea parasitic on peruvian marine fishes, with description of Anoplocotyloides chorrillensis new species and new records

The presence of four Monogenoidea parasitic on marine fishes from the central Peruvian coast is recorded. One of them, Anoplocotyloides chorrillensis (Monocotylidae) described from the gills of Rhinobatos planiceps (Rhinobatidae) is considered a new species. The three other species are: Caballerocotyla autralis Oliva, 1986 (Capsalidae); Callorhynchocotyle marplatensis Suriano & Incorvaia, 1982 (Hexabothriidae) and Anoplocotyloides papillatus (Doran, 1953) (Monocotylidae) parasitic on Sarda chiliensis chiliensis (Scombridae), Callorhinchus callorhinchus (Callorhinchidae) and Rhinobatos planiceps (Rhinobatidae) respectively.