Cyclic AMP induces differentiation in vitro of human melanoma cells.

Treating human melanoma lines with dibutyryl adenosine 3':5'-cyclic monophosphate (dbc AMP) resulted in morphologic changes associated with the altered expression of cell surface antigens. After treatment, cells developed long cellular projections characteristic of mature melanocytes and showed the presence of an increased number of Stage II premelanosomes. In addition, induction of melanin synthesis, detected as brown perinuclear pigmentation, was observed. The AMP further drastically reduced the growth rate of the five melanoma cell lines that were tested. The influence of dbc AMP was completely reversible 3 days after the agent was removed from the culture medium. The antigenic phenotype of the melanoma lines was compared before and after dbc AMP treatment. This was done with four monoclonal antibodies directed against major histocompatibility complex (MHC) Class I and II antigens and 11 monoclonal antibodies defining eight different melanoma-associated antigenic systems. Treatment with dbc AMP reduced the expression of human leukocyte antigen (HLA)-ABC antigens and beta-2-microglobulin in five of five melanoma lines. In the two HLA-DR-positive cell lines dbc AMP reduced the expression of this antigen in one line and enhanced it in the other. No induction of HLA-DR or HLA-DC antigens was observed in the Class II negative cell lines. Furthermore, dbc-AMP modulated the expression of the majority of the melanoma antigenic systems tested. The expression of a 90-kilodalton (KD) antigen, which has been found to be upregulated by interferon-gamma, was markedly decreased in all the five cell lines. A similar decrease in the expression of the high molecular weight proteoglycan-associated antigen (220-240 KD) was observed. The reduced expression of Class I and II MHC antigens as well as the altered expression of the melanoma-associated antigens studied were shown to be reversible after dbc AMP was removed. Our results collectively show that the monoclonal antibody-defined melanoma-associated molecules are linked to differentiation. They could provide useful tools for monitoring the maturation of melanomas in vivo induced by chemical agents or natural components favoring differentiation.

Estimating the basic reproductive number from viral sequence data.

Epidemiological processes leave a fingerprint in the pattern of genetic structure of virus populations. Here, we provide a new method to infer epidemiological parameters directly from viral sequence data. The method is based on phylogenetic analysis using a birth-death model (BDM) rather than the commonly used coalescent as the model for the epidemiological transmission of the pathogen. Using the BDM has the advantage that transmission and death rates are estimated independently and therefore enables for the first time the estimation of the basic reproductive number of the pathogen using only sequence data, without further assumptions like the average duration of infection. We apply the method to genetic data of the HIV-1 epidemic in Switzerland.

Executive Order Number Forty-Six, January 2006

Excecutive order signed by Governor Thomas Vilsck. Ensures Iowa's kids have a quality early childhood experience by giving child care workers the opportunity to organize. Directs the Department of Human Services to meet with authorized representatives of unregistered Child Care providers (and others not covered by Executive Order 45 )in the State of Iowa.

Executive Order Number Forty-Two, July 2005

Excecutive order signed by Governor Thomas Vilsck. Grants voting rights to disqualified electors who have discharged their sentence, probation or parole.

Executive Order Number Forty-Three, July 2005

Excecutive order signed by Governor Thomas Vilsck. Directs the Department of Human Services to meet with organizations and associations in order to improve delivery and quality of home and community-based care services for Iowa’s elderly and disabled.

Executive Order Number Forty-Four, September 2005

Excecutive order signed by Governor Thomas Vilsck. Directs the Department of Human Services to comply with all directives from the federal government arising from Hurricane Katrina insofar as those directives concern public welfare programs administered by DHS, declares that any evacuee shall be deemed a resident of the State of Iowa for purposes of obtaining eligibility for the food stamp, family investment and Medicaid programs, and DHS should process applications in an expedited and flexible manner.

Executive Order Number Forty-Five, January 2006

Excecutive order signed by Governor Thomas Vilsck. Ensures Iowa's kids have a quality early childhood experience by giving child care workers the opportunity to organize. Directs the Department of Human Services to meet with authorized representatives of Registered Child Care providers in the State of Iowa.

Executive Order Number Eleven, February 1968

Executive Orders from Governor Hughes. Establish the Department of Social Services.

Executive Order Number Forty-Seven, February 2006

Creates the Keep Iowa Clean and Beautiful Task Force to assess conditions and activities of litter control in Iowa.

Executive Order Number Forty-Eight, June 2006

Ordered the Institute for Tomorrow’s Workforce to propose a design for a pay-for-performance program and conduct a study of the design.

Executive Order Number One, May 1964

Executive Orders from Governor Hughes. Code of Fair Practices

Executive Order Number Two, October 1964

Executive Orders from Governor Hughes. All Iowa peace officers to protect President Johnson.

Executive Order Number Three, October 1964

Executive Orders from Governor Hughes. Uniform Criminal Extradition

Executive Order Number Four, June 1966

Executive Orders from Governor Hughes. Compact for education.

Executive Order Number Five, June 1966

Executive Orders from Governor Hughes. All Iowa peace officers assist during visit of President Johnson.