Danielis Cornides Vindiciae Anonymi Belae regis notarii /

Folded leaf numbered p. 371-[372] misbound between p. 368 and 369.

C. Iulii Caesaris quae extant /

"Nomenclator geographicus ... excerptus ... potissimum è Thesauro geographico Abrahami Ortelii ...": p. [437]-455.

Bibliotheca historica : oder systematisch geordnete Uebersicht ...

Editors: 10.-27. jahrg., 1861-1879, W. Müldener.--28.-30. jahrg., 1. heft, 1880-juni, 1882, E. Ehrenfeuchter.--neue folge, 1. jahrg., 1887, Oscar Masslow.

Poetae Latini minores ex recensione Wernsdorfiana : quae notis veteribus ac novis illustravit /

v. 1. Gratii et Nemesiani Cynegetica, T. Calpurnii Siculi Ecolgae, Q. Ennii, Severi Sancti, Bedae, Septimii Sereni, Ausonii, Casii Parmensis, Optatiani Porphyrii, et aliorum carmina.- v. 2. Satirica, Elegiaca, Lyrica, et alia quaedam carmina.- v. 3. Lucilii Junioris, Saleii Bassi, et aliorum carmina heroica; Epithalamia, et Homeristarum Latinorum opera, Ausonii et variorum auctorum carmina geographica, Varronis Atacini fragmenta.- v. 5. Rufi Festi Avieni Descriptio orbis terrae, Ora Maritima, et carmina minora; ejusdem Aratea Phaenomena et prognostica.- v. 6. De re astronomica Ciceronis et Germanici carmina ex Arato translata; item M. Manilii Astronomicon libri quinque ex recensione Jos. Scaligeri.- v. 7. De re Hortensi et Villatica carmina Columellae, Palladii Vomani, et aliorum; item Amatoria et Ludicra Maximiani Etrusci, Ofilii, Juventini, Sperati, Symposii, Hosidii Getae, et Ausonii. Indices varios rerum, latinitatis et nominum in septum voluminum carminibus necnon in notis, prooemiis, argumentis et excursibus occurrentium.

"Lancea", Lusitanian Word, and the "Lancienses" Ethnogenesis

Analysis of the word lancea, of Hispanic origin after Varro, and of place names, people´s names and personal names derived from it. It confirms that the spear was the most important weapon in the Bronze Age, belonging to the iuventus and used as heroic and divine symbol. This analysis confirms also the personality of the Lusitanians, a people related to the Celts but with more archaic archaeological, linguistic and cultural characteristics originated in the tradition of the Atlantic Bronze in the II millennium BC. It is also relevant to better know the organisation of Broze and Iron Age societies and the origin of Indo-Europeans peoples in Western Europe and of pre-Roman peoples of Iberia.

Tifones y gigantes en el mundo macedonio: el rey como símbolo del orden cósmico

Este artículo analiza la existencia de un posible ritual en la antigua Macedonia que fuese similar al Akîtu babilonio. Pensamos que la victoria de Zeus sobre sus enemigos en la gigantomaquia y tifonomaquia fue celebrada por los reyes macedonios para fortalecer su posición en el poder. Aunque no hay evidencias directas de ello, tenemos una serie de diferentes fuentes en las cuales los macedonios están relacionados directa o indirectamente con Tifón y los gigantes. Además, la estratégica importancia del monte Olimpo para los macedonios nos hace pensar que la victoria de Zeus sobre las fuerzas del mal fue conmemorada de alguna forma por ellos.

Revisiting molecular diagnostics of Streptococcus pneumoniae

Streptococcus pneumoniae is a human pathobiont that colonizes the nasopharynx. S. pneumoniae is responsible for causing non-invasive and invasive disease such as otitis, pneumonia, meningitis, and sepsis, being a leading cause of infectious diseases worldwide. Due to similarities with closely related species sharing the same niche, it may be a challenge to correctly distinguish S. pneumoniae from its relatives when using only non-culture based methods such as real time PCR (qPCR). In 2007, a molecular method targeting the major autolysin (lytA) of S. pneumoniae by a qPCR assay was proposed by Carvalho and collaborators to identify pneumococcus. Since then, this method has been widely used worldwide. In 2013, the gene encoding for the ABC iron transporter lipoprotein PiaA, was proposed by Trzcinzki and collaborators to be used in parallel with the lytA qPCR assay. However, the presence of lytA gene homologues has been described in closely related species such as S. pseudopneumoniae and S. mitis and the presence of piaA gene is not ubiquitous between S. pneumoniae. The hyaluronate lyase gene (hylA) has been described to be ubiquitous in S. pneumoniae. This gene has not been used so far as a target for the identification of S. pneumoniae. The aims of our study were to evaluate the specificity, sensitivity, positive predicted value (PPV) and negative predicted value (NPV) of the lytA and piaA qPCR methods; design and implement a new assay targeting the hylA gene and evaluate the same parameters above described; analyze the assays independently and the possible combinations to access what is the best approach using qPCR to identify S. pneumoniae. A total of 278 previously characterized strains were tested: 61 S. pseudopneumoniae, 37 Viridans group strains, 30 type strains from other streptococcal species and 150 S. pneumoniae strains. The collection included both carriage and disease isolates. By Mulilocus Sequence Analysis (MLSA) we confirmed that strains of S. pseudopneumoniae could be misidentified as S. pneumoniae when lytA qPCR assay is used. The results showed that as a single target, lytA had the best combination of specificity, sensitivity, PPV and NPV being, 98.5%, 100.0%, 98.7% and 100.0% respectively. The combination of targets with the best values of specificity, sensibility, PPV and NPV were lytA and piaA, with 100.0%, 93.3%, 97.9% and 92.6%, respectively. Nonetheless by MLSA we confirmed that strains of S. pseudopneumoniae could be misidentified as S. pneumoniae and some capsulated (23F, 6B and 11A) and non-capsulated S. pneumoniae were not Identified using this assay. The hylA gene as a single target had the lowest PPV. Nonetheless it was capable to correctly identify all S. pneumoniae.

AGENDA AMBIENTAL DEL AGUA EN COSTA RICA

La crisis de gobcrnabilidad de los recursos hídricos es clara y evidente en Costa Rica. Estos recursos están en proceso de deterioro y peligrosamente amenazados. En tan solo cinco décadas se pasó de la riqueza y abundancia de agua, a la vulnerabilidad y escasez de ésta. Un conjunto de realidades ha originado la mencionada crisis: la ausencia de políticas hídricas integrales, claras y estables, un marco legal desactualizado. estático y de mínimo cumplimiento, que ha permitido el uso ineficiente y la contaminación de los cuerpos de agua y desde luego la cultura dominante de poca o ninguna valoración del agua como recurso vital para el desarrollo presente y futuro de todas las especies que poblamos el país.La Agenda Ambiental del Agua en Costa Rica ha sido elaborada mediante un proceso parlicipativo. donde diversos actores sociales en diferentes regiones —Limón, Liberia y la Gran Area Metropolitana (GAM)— priorizaron los 10 principales problemas y sus potenciales soluciones:1) La educación formal e informal es muy débil respecto a como alcanzar el uso eficiente e inteligente de los recursos hrdricos. Igualmente, es débil para revertir el patrón de manejo de vertidos sin responsabilidad social.2) La excesiva centralización de las decisiones. Muy poca participación ciudadana tanto en la estructura organizativa como en la toma de decisiones.3) La inoperancia del marco institucional y legal —desconocimiento por parte de la sociedad civil de los procesos de denuncia y otros; la tramitología de denuncia es demasiado lenta e ineficiente, razón por la cual los denunciantes se cansan y desaniman—.4) La deficiente y casi nula valoración económica del agua, que tome en cuenta el manejo integral de las cuencas y la búsqueda de la permanencia del recurso en el futuro.5) La ausencia de planes hídricos de cuencas donde el plan regulador sea un componente básico.6) La desigual calidad del agua para los costarricenses según operador del servicio y región geográfica.7) La contaminación de aguas superficiales y subterráneas.8) La ausencia de conocimiento, socialización, sistematización y movilización en relación con el manejo del agua. Es necesario el aumento de las capacidades tanto técnicas y científicas como de cambio de cultura y conocimiento popular.9) Falta de información básica y mecanismos claros para poder utilizarla.10) La información existente en la actualidad se encuentra dispersa en diferentes orgarnza ciones y en algunos investigadores, lo que impide su uso efectivo.La Agenda Ambiental del Agua en Costa Rica aproxima los costos para la solución de los problemas apuntados anteriormente. Se necesita invertir alrededor de US$J .500 millones para abordar la gestión integrada de los recursos hídricos en el país.Abstract: The crisis of governance of thc watcr resources is clear and evideni in Costa Rica. These resources are in proccss oídeterioration anddangerously threatened. In only five decades it passed of che wealth and water abundance, to che vulnerability and shortagc of thc same one. A set of realities has originated dic mentioned crisis; dic abscncc of an integral, clear and siable water policy, a legal framework out of date. static one and of minimal fulfillmeni. which has allowed che inefficicrn use and ihe pollution ofihe water bodies. And certainly. hc dominecring culture of small ci no valuation to the water as vital resource for thc prcscnt and future dcvclopment of alI dic species. thai we populate che country.The Environmental Agenda of the Water in Costa Rica has bccn claborated by means of a participatory proccss. which includes several social actors in diverse regions —Limon. Liberia and Great Metropolitan Area (GAM) These actors prioritized lO principal problems related to water resources and thcir potential solutions:1) The formal and informal education is very weak with regard to since as reaches the efficient and intelligeni use of thc water resources. Equally. dic boss of managing is weak for revert of spilt without social responsibilicy.2) The excessive centralization of che decisions with very little civil parlicipation. both in the organizational siructure and in the decision making process.3) The un.workability of ihe institutional and legal framewoik —ignorance cii che pan of civil society of che proces.ses of denunciation and others the denunciation niechanism is ioo slow and inefticieni, a reason for which dic cbtmants get tired and discouragc—.4) Thedeficientone and almosi voideconomic valuationofthe waier. which bears in mmd the integral managing of the basin and dic scarc h of dic permancncy of dic resource to future.5) Thc absence of water plans of basin where dic regulatory plan is a basic component.6) The unequal quality of the water for dic Costa Ricans according tooperacor of dic service and geographica] region.7) The pollution of superficial and underground waters.8) The absence of knowledgc. socializ.arion, systematizing and mobilization in relation co the waier managing. Thc increase of dic capacities is necessary so much tcchnical and scicntific like of change of culture and popular knowledge.9) Lack of basic information and clear mechanisms to be able to use it.10) Thc cxisting information aL preseni is dispersed in different organizations and in sorne investigators, which prevents ihe effective use of the same one.The Environmental Agenda of the Water in Costa Rica brings the costs near for the solution of the pointed problems previously. Costa Rica needs to invest about US$1.500 million Lo approach ihe integrated managcment of the water resources in the country.