RefSeq and LocusLink: NCBI gene-centered resources

Thousands of genes have been painstakingly identified and characterized a few genes at a time. Many thousands more are being predicted by large scale cDNA and genomic sequencing projects, with levels of evidence ranging from supporting mRNA sequence and comparative genomics to computing ab initio models. This, coupled with the burgeoning scientific literature, makes it critical to have a comprehensive directory for genes and reference sequences for key genomes. The NCBI provides two resources, LocusLink and RefSeq, to meet these needs. LocusLink organizes information around genes to generate a central hub for accessing gene-specific information for fruit fly, human, mouse, rat and zebrafish. RefSeq provides reference sequence standards for genomes, transcripts and proteins; human, mouse and rat mRNA RefSeqs, and their corresponding proteins, are discussed here. Together, RefSeq and LocusLink provide a non-redundant view of genes and other loci to support research on genes and gene families, variation, gene expression and genome annotation. Additional information about LocusLink and RefSeq is available at http://www.ncbi.nlm.nih.gov/LocusLink/.

Argumentation schemes for policy-driven planning

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Argumentation strategies for plan resourcing

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Ecological restoration across the Mediterranean Basin as viewed by practitioners

Restoration efforts in the Mediterranean Basin have been changing from a silvicultural to an ecological restoration approach. Yet, to what extent the projects are guided by ecological restoration principles remains largely unknown. To analyse this issue, we built an on-line survey addressed to restoration practitioners. We analysed 36 restoration projects, mostly from drylands (86%). The projects used mainly soil from local sources. The need to comply with legislation was more important as a restoration motive for European Union (EU) than for non-EU countries, while public opinion and health had a greater importance in the latter. Non-EU countries relied more on non-native plant species than EU countries, thus deviating from ecological restoration guidelines. Nursery-grown plants used were mostly of local or regional provenance, whilst seeds were mostly of national provenance. Unexpected restoration results (e.g. inadequate biodiversity) were reported for 50% of the projects and restoration success was never evaluated in 22%. Long term evaluation (> 6 years) was only performed in 31% of cases, and based primarily on plant diversity and cover. The use of non-native species and species of exogenous provenances may: i) entail the loss of local genetic and functional trait diversity, critical to cope with drought, particularly under the predicted climate change scenarios, and ii) lead to unexpected competition with native species and/or negatively impact local biotic interactions. Absent or inappropriate monitoring may prevent the understanding of restoration trajectories, precluding adaptive management strategies, often crucial to create functional ecosystems able to provide ecosystem services. The overview of ecological restoration projects in the Mediterranean Basin revealed high variability among practices and highlighted the need for improved scientific assistance and information exchange, greater use of native species of local provenance, and more long-term monitoring and evaluation, including functional and ecosystem services' indicators, to improve and spread the practice of ecological restoration.

Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase

tRNAs are charged with cognate amino acids by aminoacyl-tRNA synthetases (aaRSs) and subsequently delivered to the ribosome to be used as substrates for gene translation. Whether aminoacyl-tRNAs are channeled to the ribosome by transit within translational complexes that avoid their diffusion in the cytoplasm is a matter of intense investigation in organisms of the three domains of life. In the cyanobacterium Anabaena sp. PCC 7120, the valyl-tRNA synthetase (ValRS) is anchored to thylakoid membranes by means of the CAAD domain. We have investigated whether in this organism ValRS could act as a hub for the nucleation of a translational complex by attracting other aaRSs to the membranes. Out of the 20 aaRSs, only ValRS was found to localize in thylakoid membranes whereas the other enzymes occupied the soluble portion of the cytoplasm. To investigate the basis for this asymmetric distribution of aaRSs, a global search for proteins interacting with the 20 aaRSs was conducted. The interaction between ValRS and the FoF1 ATP synthase complex here reported is of utmost interest and suggests a functional link between elements of the gene translation and energy production machineries.

Letter from Charles Stearns to Edward Wigglesworth, 1781 November 8, and receipt signed by Stephen Sewall, 1781 November 16

In a letter to Wigglesworth, acting president of Harvard and Hollis Professor of Divinity, Stearns resigns his tutorship and writes that he will return an unnamed silver tankard and various books to the College. The folder also includes as a receipt for those objects upon their return, signed by Harvard Professor Stephen Sewall.

Letter from Oliver Wendell to John Thornton Kirkland, 1817

One letter offering Harvard President Kirkland a donation of 100 dollars to acquire a "handsome Christening Cup or Baptismal Basin" for the new College Church, which held services in University Hall.

Twentieth century manuscript copies of correspondence between Thaddeus William Harris and Edward Everett, 1847 August

One letter from Harris, the University Librarian, to President Everett, enclosed with a historical account of the Great Salt and its donor, Richard Harris, and sketches of the new engravings on the Great Salt, Stoughton Cup, and Browne Cup bearing donor names. Harris writes that he hopes to have his account of the Great Salt published in the Cambridge Chronicle and is gratified to hear of Everett’s plans to use an excerpt in his Commencement dinner speech. In a short note of reply, Everett writes that Harris’ account of the silver was "received with great favor" during the dinner.

Memorandum Concerning the Holyoke Bowl, 1904 June 14

One-page typed document reflecting the donation of the Holyoke Caudle Cup by Charlotte A. Hedge in 1903. Provenance information is also included. There is a handwritten note at the bottom of the page regarding the cup’s inclusion in an exhibition catalog published by the Boston Museum of Fine Arts.

Letter from Walter Kendall Watkins, 1905 October 21

One letter addressed "Dear Sir," enclosed with a one-page biographical sketch of Richard Harris. Walters indicates in the letter he had been permitted to examine the college silver, including the Great Salt, in the spring of 1905.

Sundrypeices of the Plate given to the College, 1736 September 18

One-page inventory of the silver, including the weight of each object, donor, and dates; one passage is crossed out, and written diagonal to the inventory are the words "Fort London Farmer London." Docketed on the verso with an address to the Right Honorable Samuel Bishop of York.

Committee Report on College Silver, 1781

Report of a committee appointed by the College to inventory the College Plate, listing faculty members and the pieces in their possession. The folder also includes two receipts signed by Harvard tutors Charles Stearns (Harvard AB 1773) and William Bentley (Harvard AB 1777, AM 1780), acknowledging the vessels they were loaned.

Twentieth century transcription of inventories by Thaddeus William Harris, 1845 July 25-1852 July 3, and undated

Transcription of inventories of College silver completed by Harris in 1845 and 1852, as well as an undated list of silver.

Inventory of Silver Plate, Belonging to Harvard College, and in the Keeping of the President, 1886 December 8

Black and white composition book sent to the Harvard College Library containing a typed "copy of notes made in the spring of 1886" by John H. Buck. Includes historical information, and physical descriptions and valuations of the Great Salt, the Stoughton Cup, the Browne Cup, and the christening basin acquired with the donation of Oliver Wendell, as well as notes on other gifts of silver.

III. Research materials used in William C. Lane's article, "Early Silver Belonging to Harvard College," [1923]

This folder contains transcriptions of archival materials used in Lane's research for the article, published by the Colonial Society of Massachusetts in 1923. Included are two letters from the papers of Harvard President Jared Sparks (1849-1853) regarding the christening basin and the role of the College steward in the care of the silver collection; and a 1781 inventory (see also in folder 7) and 1829 Corporation vote excerpted from College records. There are also two notes containing citations.