968 resultados para Enxerto de tecido
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Pós-graduação em Zootecnia - FCAV
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Pós-graduação em Medicina Veterinária - FMVZ
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Patologia - FMB
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It was evaluated the influence of rootstocks 'IAC 766', 'IAC 572', 'IAC 313', 'IAC 571-6' and 'Ripária do Traviú' and the pruning periods in the duration of phenological stages and in the accumulation of degree-days on grape 'Niagara Rosada' .The experiment was conducted in Louveira, SP. The treatments consisted of five rootstocks and three times of pruning, the experimental design being used in a completely randomized split plot with five replications, and plots represented by rootstocks and the subplots were pruning. After pruning the grapevine, were realized evaluations for phenological stages, using the criterion of Eichhon e Lorenz (1984). ). In three seasons of pruning, based on phenological stages, it was calculated the length of the periods: pruning to budding, pruning to the beginning of the flowering, pruning to the beginning of fruiting, pruning to beginning of ripening of the bunches, pruning the beginning of the harvest. Taking as a basis the cycle length of the vine and the average daytime temperatures, it was calculated the accumulation of degree-days. Data were submitted to ANOVA and Tukey test at 5% significance level. It was obtained in the summer pruning the reduction in duration of the phenological stages of 'Niagara Rosada' grafted on the rootstock 'Ripária Traviú'. The longer duration of the cycle and accumulation of degree-day of 'Niagara Rosada' cultivar were obtained with the rootstocks 'IAC 572' and 'IAC 313' pruning in the winter.
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The garlic (Allium sativum L.) can be naturally infected by a complex of filamentous viruses belonging to the genera Potyvirus, Carlavirus and Allexivirus. Accumulation of these viruses occurs especially by vegetative propagation through cloves. As the cultivated garlic plant does not produce true seed worldwide, virus-free plants can only be obtained by tissue culture of stem apices and thermotherapy. Using these techniques, garlic seeds were produced at the School of Agricultural Sciences - UNESP, Botucatu, and evaluated by RT-PCR for the presence of potyvirus, carlavirus and allexivirus. In the second generation of microcloves propagated in a greenhouse, 6.6% infection was detected, only by allexivirus. In the fourth generation, however, there was 60% incidence by allexivirus, 35% by potyvirus and all negative by carlavirus. The high rate of infection by allexivirus may be related to the greater difficulty of removing the species of viruses belonging to this genus, as observed by other authors, and also based on the infection and transmission of the virus by the mite, Aceria tulipae, during the storage of bulbs from one year to the other. The garlic at the fourth generation corresponds to cloves weighed less than 1 gram and not selected for commercial multiplication. Selection for the size of cloves has a positive effect on the choice of cloves with lower rates of viral infection, as the technique of thermotherapy and tissue culture do not eliminate the virus completely. Results also emphasize the need of fumigation for the garlic seed stored from one year to the other in order to prevent the transmission of allexivirus during storage.
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Pós-graduação em Biologia Geral e Aplicada - IBB
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Ciências Odontológicas - FOAR
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Pós-graduação em Odontologia - FOAR
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Pós-graduação em Biotecnologia Animal - FMVZ
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Pós-graduação em Odontologia - ICT
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This research objective was to verify the tissues reactions obtained, in surgical experimental bone defect, after the etil cyanoacrylate chemical adhesive application in rats parietal bones. 1t was investigated the acceptance or no acceptance of the tissues surround the bone defect in which was placed the etil cyanoacrylate and the control bone defect. The specimens were submitted to light microscopic analysis. 24 adults rats were used (Rattus norvergicus, albinus, Wistar) with the average weight to 300 gr. and were divided in two groups: treated and control. After the bone defects were realized, etil cyanoacrylate was placed over one of the defects at the parietal bone (treated group). On the other defect nothing was placed and it was used like control group. After 24 hours, 3,5,7,30 and sixty days after surgery, for animals of each group were sacrificed. The bone samples were removed and fixed in 10% formalin during 72 hours and analyzed using light microscopy. The results showed that the acute inflammatory responses was more observed at lhe treated group than the control group. The chemical adhesive was observed until 60 days follow the surgery and the tissues around it present normal appearance. The chemical adhesive, etil cyanaocrylate, did not impede at the bone repair process
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)