Group of two women, one man and one child, sit on chairs outdoors Portraits Groups

Digital Image

Group of two women, one man and one child, sit on chairs outdoors Portraits Groups

Digital Image

Group portrait of the American Jewish Joint Distribution Committee; Shanghai Portraits; Groups

Photograph contained within portfolio including title page and signatures

Group portrait, 2nd reunion of the World War II 75th Station Hospital; Hotel Governor, Clinton, New York Portraits Groups

Digital Image

Group portrait, welcome home dinner for ex-service personnel; Newark Beth Israel Hospital; Hotel Essex House, Newark, New Jersey Portraits Groups

Digital Image

Group portrait, testimonial dinner for Dr. Paul Klemperer's 10th anniversary at Mt. Sinai Hospital given by his students; Cavanagh's, New York City Portraits Groups

Digital Image

Phylogenetic lineage and pilus protein Spb1/SAN1518 affect opsonin-independent phagocytosis and intracellular survival of Group B Streptococcus

Opsonin-independent phagocytosis of Group B Streptococcus (GBS) is important in defense against neonatal GBS infections. A recent study indicated a role for GBS pilus in macrophage phagocytosis (Maisey et al Faseb J 22 2008 1715-24). We studied 163 isolates from different phylogenetic backgrounds and those possessing or lacking the gene encoding the pilus backbone protein, Spb1 (SAN1518, PI-2b) and spb1-deficient mutants of wild-type (WT) serotype III-3 GBS 874391 in non-opsonic phagocytosis assays using J774A.1 macrophages. Numbers of GBS phagocytosed differed up to 23-fold depending on phylogenetic background; isolates possessing spb1 were phagocytosed more than isolates lacking spb1. Comparing WT GBS and isogenic spb1-deficient mutants showed WT was phagocytosed better compared to mutants; Spb1 also enhanced intracellular survival as mutants were killed more efficiently. Complementation of mutants restored phagocytosis and resistance to killing in J774A.1 macrophages. Spb1 antiserum revealed surface expression in WT GBS and spatial distribution relative to capsular polysaccharide. spb1 did not affect macrophage nitric oxide and TNF-alpha responses; differences in phagocytosis did not correlate with N-acetyl d-glucosamine (from GBS cell-wall) according to enzyme-linked lectin-sorbent assay. Together, these findings support a role for phylogenetic lineage and Spb1 in opsonin-independent phagocytosis and intracellular survival of GBS in J774A.1 macrophages.

heilbrunn fritz; card to heilbrunn karl; group of unidentified soldiers Military; Portraits

correspondence: card from fritz to karl; returned 4/ 16

Professor Della Giovanna with unidentified group outside of the entrance to Madonna Del Cufo Church; Rome Portraits Groups

Digital Image

Ermano Loevinson, leader of excursion, with unidentified group members; Ostia Portraits Groups

Digital Image

Unidentified group; Rome Portraits; Groups

Digital Image

Group of young school girls with teacher [?].

Note in German on back.

Albert Einstein with unidentified group at outdoor gathering Portraits; Groups

Digital Image

Albert Einstein sitting with unidentified group at outdoor gathering Portraits; Groups

Digital Image

Traveling Group of Men, Horses and Wagons

Digital image