Desempenho de Ankistrodesmus gracilis (Reischi) Korsikov (Chlorophyta) cultivada em três meios de cultura em laboratório

Pós-graduação em Aquicultura - FCAV

Geoestatística multivariada na delimitação de zonas de manejo na cultura de cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Indução de morte celular por apoptose decorrente da irradiação com Raios-X: um ensaio com células mamárias humanas tumorais

Apoptosis is a form of programmed cell death selectively removes abnormal cells, and thus contributes to maintaining the balance of the dynamics of cell reproduction. Therefore the verification of the occurrence of apoptotic cell death after a pathological stimulus is crucial for the analysis of the maintenance of normal cell cycle of a given tissue or organ. In this experiment were used cells lines human mammary tumor MDAMB231, T47, MCF7, which were irradiated with X-rays at a dose of 5 Gy in a time interval of 15 seconds, and filtration of 1mm aluminum. Samples containing the cells were grown in a specific culture medium, containing fetal bovine serum and growth factor, and two samples were prepared with each of the cell lines, one to be irradiated, and another that has not been irradiated, which denoted by negative control of the irradiation. The primary goal of the experiment was to verify and compare the rates of apoptosis in each cell lines, in which were irradiated and that were not irradiated, using flow cytometry as a method for detecting apoptotic cell death in together with specific markers annexin V and propidium iodide. Data from the readings made by flow cytometry were analyzed and interpreted using the software WinMDI statistical graph. By comparing the indices relating to the readings of positive and negative for specific markers of apoptosis, based on differences in the statistical data presented lectures regarding the cellular irradiated and not irradiated, collude cells in question once... (Complete abstract click electronic access below)

Sensoriamento remoto e Sistemas de Informações Geográficas (SIG) na análise dos impactos referentes à cultura canavieira e reflorestamento: a subtração dos biomas nativos no município de Itirapina-SP

The main objective of this study was to perform a temporal analysis of land use and cover of Itirapina – São Paulo estate, Brazil, for 1962, 2000 and 2008 scenarios, indicating the rate of change in native vegetation and contextualizing its modifications. Based on photointerpretation performed by supervised classification using the method of Bhattacharya, the thematic classes were mapped and characterized in an area equivalent to 56400 hectares. Using remote sensing technologies and geographic information systems, was structured and implemented a georeferenced and relational database, allowing the analysis of the size of the area occupied. The results showed that the expansion of cultivation of cane sugar and reforestation in the past 45 years has been conditioned the fragmentation of natural vegetation cover in the city, which fell by 34793 ha (61,7%) in 1962 to 9765,2 ha (17,3%) in 2008, with the loss of 25027,8 hectares.

Marcadores de superfície de Células-tronco: estudo de reação cruzada inter-espécies

Mesenchymal stem cells (MSCs) are adult multipotent cells with fibroblastoid morphology and adherent to plastic. Furthermore, they can be obtained from different sources. Besides bone marrow, these cells are taken from umbilical cord blood, umbilical vein, saphenous vein, peripheral blood, arteries, liver and fetal pancreas, placenta, dental pulp and adipose tissue. MSCs derived from adipose tissue are important because of the abundant number of cells that can be obtained from this tissue, easy access and little discomfort to the patient. This study compared two techniques for obtaining MSCs from adipose tissue: mechanical dissociation (MD) and enzymatic digestion (ED). We also analyzed the inter-species cross-reactions using commercial monoclonal antibodies directed against surface antigens of stem cells from different species: mouse, horse, rabbit, monkey and human. We found that MD technique is favorable in relation to ED within 15 days of culture, and ED is more efficient in the first days of culture. The data also showed that MD causes less damage to cellular DNA. About inter-species cross-reactions, the monoclonal antibody A69 directed against stem cells from rabbits, which can be used in veterinary medicine, particularly in research involving horses

Cultivo celular in vitro: importância para a pesquisa biomédica e dimensão da problemática de autenticação de linhagens celulares

Experimental models composed by human and animal cell lines are simplified and informative, allowing them to be widely used for biomedical research. Most laboratories that use in vitro cultivated cells maintain a variation of cell lines stored and cultivated. Therefore, misidentification and cross-contamination events can happen during cell lines handling. This problem can generate a repertoire of dubious results and papers, which may prejudice biomedical research. Recently it was created the International Cell Line Authentication Committee (ICLAC), which aims to spread knowledge about cross-contamination and misidentification of in vitro cell lines. Despite of the efforts spent trying to aware scientific community about the importance of the correct identification of cells, the number of papers based on misidentified cell lines it´s still worrying, compromising the reliability of out coming results and conclusions regarding them. The present study aims to analyze and discuss the main advantages and limitations of eukaryote in vitro cell lines use, characterizing the cell lines authentication problems. Therefore, compilation and critical analyses of literature data was realized, aiming to improve the understanding about this subject. Based on information about 445 cell lines with issues published by ICLAC it´s clear that contamination in human cell lines represented 89,2 % of mentioned problems. HeLa cell line was the responsible for most contamination, especially in 92 normal tissue cell lines, representing 44,6% of the contamination. These results reinforce the importance of periodic maintenance of cell lines cultures by labs and implementation of authentication methods as polymorphic STRs, besides obtaining cell lines from reliable sources and cell banks

Caracterização de Bacteriófagos Infectando Isolados Clínicos de Pseudomonas aeruginosa Armazenados em uma Coleção de Cultura

To isolate, to concentrate and to purify bacteriophages from isolates of P. aeruginosa; To observe the capacity of bacteriophages to infect isolates of P. aeruginosa susceptible and multiresitant to antimicrobial; To caractherize bacteriphages by electronic microscopy techniques. 10 isolates of Pseudomonas aeruginosa from LEMC culture collection were submitted to the experiments of ideal temperature for the lyse region appearance in the MaConkey culture plate and 2 extraction methods for the concentration of the phages, clorophorm (Silankorva) and filtration plus centrifugation (Bergan). Three infected clinical isolates of multiresistant P. aeruginosa an one susceptible isolate ( PA01) were evaluated by 3 transmission electron microscopy techniques to caractherize phages morphologically (“on grid”, “on drop” and direct extraction from the lyse region of the culture plate). The ideal temperature to obtain lyses region was 37°C. The stock solutions, obtained through the methodologies of Sillankorva and Bergan, had satisfactory results in infecting the multiresistant isolate and the negative control. Among the 3 techniques of electronic microscopy tested the direct from the lyse plate was the best to obtain the micrography of the phages

As relações entre desenho infantil e cultura

As diferenças de desenho infantil entre os gêneros feminino e masculino têm se tornado um instigante tema de pesquisa educacional nos últimos anos entre os pesquisadores da área, buscando maior aprofundamento, conhecimento das técnicas e das formas como têm sido concebidas as produções infantis através dos anos, levando em conta o contexto diverso em que se situam tais produções. Pretendeu-se, por meio de pesquisa bibliográfica, buscar dados relevantes que pudessem orientar o aprofundamento das relações entre desenho infantil e cultura. O estudo do desenho infantil tem sido explorado por teóricos, e, a partir de informações já coletadas pela produção acadêmica existente, pretendeu-se acrescentar novas formas de observação e elucidação das produções infantis, analisandoas sob o viés da cultura. Um dos principais objetivos foi o de verificar, como tais produções vêm se desenvolvendo através da história, tendo em perspectiva a análise dos elementos cotidianos da vida da criança nessas produções, analisando inclusive, se a mídia atua de alguma forma sobre a produção dos desenhos e/ou se a produção infantil é direcionada pelos adultos do contexto ao qual a criança se insere. Por meio desta pesquisa foi possível verificar como a questão do gênero influencia as produções dos desenhos infantis

Comparação das técnicas semi-quantitativa e quantitativa nas infecções relacionadas a cateter em neonatos e determinação da resistência à oxacilina nos Staphylococcus spp

Study the semi-quantitative and quantitative technique in the diagnosis of catheter-related infections in newborns and to determine oxacillin resistance in Staphylococcus isolated. It was analyzed 353 catheter tips from 273 newborns in the Neonatal Unit of Hospital FMB. To confirm the diagnosis of infection, were analyzed the clinical data of newborns, the presence of at least one positive blood culture and growth of ≥ 1000 CFU/mL on quantitative culture and/or ³15 UFC on semiquantitative culture, with the same microorganism isolation (species and drug sensitivity) in blood culture and no other focus of infection except the catheter. The disk diffusion technique was used to check similarity of strains and resistance to oxacillin. Of the 353 tips analyzed, 39 were included in this study as the inclusion criteria. The semiquantitative culture was positive in 26 (66.7%) catheters and quantitative culture was positive in 24 (61.5%). Of 273 patients, 19 (6.9%) had a diagnosis of catheter-related bloodstream Infection (CR-BSI). Of the 19 episodes of CR-BSI, S. epidermidis was the predominant etiological agent (84.2%). The resistance to the antibiotic methicillin was found in 14 (73.7%) strains of Staphylococcus. The semiquantitative method was more sensitive (79%) compared with the quantitative method (63%). The use of antibiotics may have influenced the sensitivity of the quantitative method as the microorganisms present in the lumen are exposed to higher concentrations of antibiotics administered via the catheter

As técnicas de agricultura tradicional em combinação com propostas e práticas de sistemas agroflorestais: consensos possíveis ao desenvolvimento sustentável da Reserva de Desenvolvimento Sustentável Quilombos de Barra do Turvo, Vale do Ribeira (SP)

O presente estudo teve como objetivo analisar questões sociais e econômicas do cotidiano de quatro comunidades quilombola (Cedro, Pedra Preta, Ribeirão Grande e Terra Seca) residentes da Reserva de Desenvolvimento Sustentável Quilombos de Barra do Turvo (Vale do Ribeira/SP), e levantar o debate acerca da questão da luta pelo reconhecimento da posse da terra frente à saída dos mais jovens da comunidade para as grandes cidades. Através dos dados coletados por meio de levantamento bibliográfico a respeito do tema e entrevistas semiestruturadas com representantes das famílias das quatro comunidades, foi possível discorrer a respeito da problemática da saída dos jovens, que afeta diretamente o futuro dessas comunidades tradicionais, bem como a sugestão de alternativas que visando geração de renda para essas famílias e a inserção e o futuro estabelecimento desses jovens no cotidiano das comunidades. Os resultados deste trabalho mostram que esses quilombolas, bem como seus antepassados, formam comunidades essencialmente rurais e agrícolas, com dinâmica fortemente ligada ao meio natural circundante. Quanto às suas práticas agrícolas, as famílias se dividem em dois grupos: os praticantes da agricultura tradicional, também chamada de “coivara”, e a agroecologia, com Sistemas Agroflorestais bem estabelecidos, onde os agricultores são organizados através de uma cooperativa agroflorestal. Essa divisão agrícola, como mostrado na pesquisa, tem reflexo direto na organização social e econômica das comunidades, bem como no processo de preservação do patrimônio cultural desses grupos. A pesquisa apontou ainda para um grande êxodo dos indivíduos mais jovens das quatro comunidades rumo aos grandes centros urbanos, pela falta de interesse destes na cultural local, ou pela busca por empregos regulares, levantando o questionamento sobre a preservação da cultura e do território quilombola de Barra do Turvo

Afirmação do projeto ético-político do serviço social: a utilização das técnicas das manifestações artístico-culturais no debate sobre os movimentos sociais

Pós-graduação em Serviço Social - FCHS

Resveratrol como meio de estocagem para dentes avulsionados: estudo in vitro da viabilidade celular

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção otimizada de biocelulose utilização de biologia molecular de micro-organismos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Aplicação da membrana de látex natural (NRL) como suporte para cultura das células osteogênicas MC3T3-E1 para regeneração óssea guiada (GBR)

Pós-graduação em Biotecnologia - IQ

Efeito citotóxico de agentes clareadores a base de peróxido de hidrogênio a 20% e 38% sobre células odontoblastóides

The aim of this in vitro study was to evaluate the trans-enamel and transdentinal cytotoxic effects of two in-office tooth bleaching techniques that employ bleaching gels containing 20% and 38% of H2 O2 on cultured odontoblast-like cell line (MDPC-23). Sixty enamel/dentin discs were obtained from bovine central incisors and placed individually in artificial pulp chambers. Six groups were formed according to the following enamel treatments: G1- 20% H2 O2 (1 application); G2- 20% H2 O2 (2 applications); G3- 38% H2 O2 (1 application); G4- 38% H2 O2 (2 applications); G5- 38% H2 O2 (3 applications); and G6- control (no treatment). In G1 and G2, the bleaching gel was left in contact with the enamel surface for 45 min in each application. However, in G3, G4, and G5 the bleaching gel was applied for only 10 min per application. After the last application, the extracts were collected and applied on previously cultured cells (30.000 cells/cm2 ) for 24 h. Cell metabolism was evaluated by the MTT assay and cell morphology was analysed by scanning electron microscopy. Cell metabolism decreased by 96.29%; 96.11%; 96.42%; 95.62%; and 97.18% in G1, G2, G3, G4, and G5, respectively. All treated groups differed significantly from non-treated control group (G6) (p < 0.05). However, the difference in cell metabolism among treated groups was not significant statistically. In addition, significant morphological cell alterations were observed in all treated groups. Under the tested experimental conditions, the extracts collected after both tooth bleaching techniques evaluated in this study caused severe toxic effects on cultured odontoblast-like cell MDPC-23.