993 resultados para Controle em tempo real


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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea) is a substituted urea herbicide widely used in crops of sugar cane, cotton and soybeans. In 1997, this agent has been classified by the United States Environmental Protection Agency as known/likely human carcinogen because it induced tumors in the urinary bladder and renal pelvis of rats, and breast and skin of mice exposed to 2500 ppm for feed for two years. A previous study from our group demonstrated dose-response relationship in the gene expression profile associated with severe necrosis on bladder urothelium and increased incidence of simple hyperplasia in male Wistar rats treated with different concentrations of diuron for 20 weeks. To check how early the molecular changes occurs, rats were fed for 7 days with diets containing diuron at 0, 125, 500 or 2500 ppm. The main observations recorded were urothelium ultrastructural alterations and disruptions of molecular pathways associated with cell-cell interaction and the tissue organization maintenance. Particularly, the gene Glypican 3 (Gpc3), a surface proteoglycan related to cellular adhesion and apoptosis induction, was down regulated on urothelium exposed to 2500ppm diuron for 7 days and 20 weeks. The aim of this study was validate by quantitative RT-PCR real time, the reduced Gpc3 gene expression in epithelial cells of the urinary bladder of male Wistar rats treated with different concentrations of diuron for 7 days and 20 weeks. The endogenous control of the quantitative PCR real time technique was the β-actin gene and the target was the gene Gpc3. The relative quantification (RQ) was obtained by the method of relative quantification 2-ΔΔCt . Animals exposed to diuron for 7 days or for 20 weeks presented reduction of Gpc3 gene expression compared to the control group. This reduction was statistically significant only for the 7 days study. Moreover, by comparing animals exposed for 7 days with the exposed for 20 weeks, it was ...

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The use of biomass as an energy source has been increasing in Brazil, with emphasis on the use of wood biomass, such as bark, wood chips and sawdust, that after receiving the appropriate treatment can be used in burners for power generation. However, from this burning are emitted fine particles known as particulate matter and a wide range of toxic organic and inorganic components in the form of gases that contribute greatly to air pollution and global warming, affecting human health, the environment and climate. The objective of this project was the quantification of gaseous and particulate, using and evaluating the equipment DR4000 ( Dataram 4 ) sampling of particulates smaller than 2.5μm and EUROTRON ( Ecoline 4000 ) when sampling gaseous pollutants, emitted from the burning of biomass in real time in the firing burner to a chimney attached biomass . We note that there are no specific rules that establish emission limits for particulate matter with diameter less than 2.5μm that are most harmful to human health and the highest concentrations reached about 800000μg/m3, for smaller diameters. It is noticeable the need for sampling of pollutants especially in industries that use biomass to fuel that could be implanted emission control equipment

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Due to the need of more agility, dynamism and reliability in stock management, the market has turned its attention to RFID, which offers a practical solution and live up to the big corporations' expectations. The search for continuous improvement establishes a direct link with RFID, for its technology offers accurate real time information in any monitored given place. RFID is a radio frequency communication technology. A reader communicates to a tag through waves. This tag can be active, with a battery, or passive, in which its power is supplied by currents induced by the field variation it is exposed to. The RFID technology has a well defined basic principle, but there are many constructive ways of implementing it. The adaptability as well as the flexibility regard to the several kinds of stock in industry, reaching their particular needs. RFID can lower maintenance costs in stock by collecting data more accurately, lowering human error and optimizing the item handling at its reception, exit and while it is inside the company. This paper presents an example of RFID technology applied in a company's stock management. It is possible to prove that there are, in fact, great advantages in implementing this technology, as it creates the opportunity for continuous improvement in stock management, with better application of material, labour and quality tools, since the data collected through an automatized process using RFID are more reliable and dynamic

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A insuficiência cardíaca (IC) está associada à miopatia dos músculos esqueléticos com aumento da expressão das isoformas rápidas da cadeia pesada de miosina e alterações na matriz extracelular. Os fatores de regulação miogência(MRF),como a MyoD e MRF4, pertecem a uma família de fatores transcricionais que controlam vários genes músculo-específicos.Esses fatores forma heterodímeros com proteínas HLH e ligam-se a seqüências de DNA conhecidas como Ebox, presentes na região promotora de vários genes músculo–específicos, incluindo todos aqueles que codificam as subunidades dos receptores nicotínicos de acetilcolina (nAChR) da junção neuromuscular (JNM). Baseado no fato de que na IC há uma diminuição na expressão da MyoD e MRF4 no músculo sóleo de ratos com IC induzida por monocrotalina, o objetivo do presente trabalho é verificar possíveis alterações na expressão dos receptores de acetilcolina, bem como realizar uma análise morfológica e morfométrica das JNMs. Neste estudo foram utilizados ratos Wistar, machos, divididos em grupos controle e experimental e a Insuficiência cardíaca (IC) foi induzida pela injeção de monocrotalina intra-peritoneal.Quando os sintomas de IC foram visualizados (após 22 dias) os animais foram sacrificados pentobarbital sódico i.p. (50 mg/Kg). A seguir foram mensurados o peso corporal (PC) dos ratos, bem como outros parâmetros. O músculo sóleo de ambos os antímeros foram dissecados e preparados para: avaliação da expressão gênica das subunidades ε, γ e α dos receptores de acetilcolina por PCR em Tempo Real; para análise morfológica e morfométrica da Junção Neuromuscular através da técnica de Esterase inespecífica; para análise ultra-estrutural da JN. Os resultados indicam que não houveram alterações morfológicas e morfométricas na JN, mas houve um aumento... (Resumo completo, clicar acesso eletrônico abaixo)

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As membranas corioamnióticas são barreiras mecânicas contra a ascensão de microrganismos e possuem papel fundamental no sistema imune inato. Quando é invadida por microrganismos apresenta corioamnionite aguda, que é a infiltração das membranas fetais por leucócitos polimorfonucleares. Entretanto as membranas têm efeito inibitório sobre o crescimento de muitas bactérias, em parte, pela produção de defensinas. Quantificar a expressão de defensinas (HBD1, 3 e 4) por membranas corioamnióticas de gestações complicadas por corioamnionite. Foram incluídos no estudo 40 fragmentos de membranas corioamnióticas, com diagnóstico histológico de corioamnionite, provenientes de gestações complicadas por rotura prematura de membranas pré-termo (RPM-PT) e/ou trabalho de parto prematuro (TPP) que apresentaram parto prematuro como desfecho gestacional, constituindo o grupo estudo (G1). Como grupo controle (G2), foram avaliadas 40 membranas corioamnióticas, com ausência de corioamnionite e pareadas pela idade gestacional ao grupo estudo. Fragmentos das membranas corioamnióticas foram encaminhados à análise histopatológica para confirmação de corioamnionite histológica. Outros fragmentos de 1cm2 das membranas foram acondicionados em RNA later e submetidos à extração de RNA total. Após a extração do RNA, as amostras com concentração entre 0,02 e 0,2μg/ μL de RNA foram submetidas à obtenção de cDNA e posterior utilização na quantificação da expressão de defensinas pela técnica da PCR em tempo real empregando-se o sistema TaqMan® Gene Expression Assays. Em relação às variáveis sócio-demográficas, as porcentagens de mulheres que referiram união estável, cor branca e hábito tabagista foram similares entre os grupos estudados. Em relação às variáveis obstétricas não houve diferença estatisticamente significativa entre os grupos G1 e G2... (Resumo completo, clicar acesso eletrônico abaixo)

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Changes are occurring worldwide, including the area of positioning using Global Navigation Satellite Systems in mobile devices such as mobile phones or laptops. This is due to the great improvement and availability of Internet services to these mobile devices. The accuracy and speed of data transmission for these devices makes the technology of sending / receiving data via an internet targeted for optimization. The optimization could allow obtaining, in real-time, coordinates (latitude, longitude and altitude) of suitable quality for users of GPS (Global Positioning System) devices that have wireless Internet access, such as those used to control the eet, locomotion, navigation, agriculture, etc. . The use of the protocol NTRIP (Networked Transport of RTCM via Internet Protocol) in GPS applications is growing every day. Applications are available in C, which creates an opportunity for development with a focus on multiplatform environments. In this context, we propose an application, implemented in a multiplatform environment and the use of NTRIP, able to run on a mobile device for receiving GNSS data

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A virtual studio system can use technologies as augmented reality and digital matting to decrease production costs at the same time it provides the same resources of a conventional studio. With this, it’s possible for the current studios, with low cost and using conventional devices, to create productions with greater image quality and effects. Some difficulties are recurrent in virtual studio applications that use augmented reality and digital matting. The virtual objects registration in augmented reality techniques suffer from problems caused by optical distortions in the camera, errors in the marker tracking system, lack of calibration on the equipments or on the environment (lighting, for example), or even by delays in the virtual objects display. On the other hand, the digital matting’s main problem is the real-time execution to preview the scene, which must have optimized processing speed at the same time while maintain the best image quality possible. Taking the given context into consideration, this work aims to give continuity to a virtual studio system called ARStudio, by enhancing digital matting, virtual objects registration and introducing a segmentation based on depth map, yet adding better control over functionalities previously implemented

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Pós-graduação em Medicina Veterinária - FCAV

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The use of mobile robots in the agriculture turns out to be interesting in tasks of cultivation and application of pesticides in minute quantities to reduce environmental pollution. In this paper we present the development of a system to control an autonomous mobile robot navigation through tracks in plantations. Track images are used to control robot direction by preprocessing them to extract image features, and then submitting such characteristic features to a support vector machine to find out the most appropriate route. As the overall goal of the project to which this work is connected is the robot control in real time, the system will be embedded onto a hardware platform. However, in this paper we report the software implementation of a support vector machine, which so far presented around 93% accuracy in predicting the appropriate route.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)