Larval development of a semi terrestrial mangrove sesarmine crab Chasmagnathus convexus (Crustacea: Decapoda: Brachyura: Grapsidae) reared in laboratory

Four zoeal stages and one megalopal stage were identified in laboratory reared semiterrestrial mangrove sesarmine crab Chasmagnathus convexus. At an average salinity and temperature of 20±1% and 19.2±0.2°C, the megalopa was attained 24 days after hatching. Morphologically, the first zoae of C. convexz1s is very similar to those of other species of the genus Chasmagnathus as well as species of the genus Helice, in that view all share the following characteristics: lateral spine on the carapace, three pairs of setae on the posterior margin of the telson furca, one plus five setae on the endopod of the maxillule, and two plus two setae on the endopod of the maxilla. The differences between the first zoea and megalopa of and those of its congeners are discussed.

Population structure of the grapsid crab, Helice tridens latimera (PARISI) in the Taiho mangrove, Okinawa, Japan

Grapsid crab Helice tridens latimera inhabiting mangroves, seashores as well as muddy and rocky areas. Ovigerous females were observed from December to May. Juveniles appeared in July and from December to April. In the laboratory they reached 9.50 mm in carapace width 4 months after hatching. It is likely that spawning of this crab occurs throughout the year.

Reproductive biology of freshwater crab, Paratelphusa spinigera, Wood Mason (Crustacea-Decapoda)

This study was aimed to analyze the annual reproductive cycle of the freshwater crab Paratelphusa spinigera (Wood Mason, 1871). P. spinigera breeds only once in a year; hence, it is a monovoltine species. Gonad maturation, changes in abdomen shape, size and female maturity index (FMI) marked the onset of sexual maturity of female P. spinigera. The occurrence of berried females marked the onset of breeding season. The fecundity of P. spinigera ranged from 533 to 1306 in number, with an average of 699.11 ± 217.38. The correlation of fecundity with carapace width and body weight was also found to be positively significant (r = 0. 780 and 0.933, respectively). The eggs were carried on the pleopods and nurtured for approximately 30-35 days, until the eggs hatch, showing perfect maternal care. The FMI values ranging between 0.70 and 0.80 represented immature stage of gonadal development. When the FMI ranged from 0.91 to 1.00, all stages of gonadal development, i.e. developing, maturing and mature stages were observed. The females with fully ripe ovary had FMI values greater than 1.00.

Feasibility of bi-culture of mud crab (Scylla serrata) and shrimp (Penaeus monodon)

A study on the feasibility of bi-culture of mud crab (Scylla serrata) and shrimp (Penaeus monodon) in brackishwater earthen ponds (0.1 ha each) was carried out for a period of five months (March-August). Nursed shrimp juvenile (ABL:· 3.36±0.23 em and ABW: 0.26±0.04 g) and crab juvenile (ACL: 2.61±0.22 cm, ACW: 4.63±0.11 cm and ABW: 43±2.64 g) were stocked following the experimental design of shrimp 2/m2 (Treatment-1), shrimp 2/m2 and mud crab l/m2 (Treatment-2) and shrimp 2/m2 and mud crab 0.5/m2 (Treatment-3). Crabs were fed with chopped trash tilapia @ 10~5%, while shrimp were fed with Saudi-Bangla shrimp feed @ 3~5% of biomass twice daily. Significantly (p<0.05) higher specific growth rate (SGR) of shrimp and mud crab was 1.86% (g/day) in T2 and 0.83% (g/day) in T3, respectively. The survival of shrimp and mud crab also varied significantly (p<0.05) with a higher mean value of74.63% in Tl and 51.04% in T3, respectively. The production of shrimp (424.09 kg/ha) was significantly (p<0.05) higher in Tl and that of mud crab (568.80 kg/ha) in T2. Significantly (p<0.05) highest total production of 871.29 kg!ha was in T2 followed by 708.52 kg/ha in T3 and 424.09 kg/ha in Tl. The results indicate that mud crab can be cultured at a stocking rate of 1/m2 together with shrimp at 2/m2 •

Effect of dietary supplementation of tocoferol acetate alone and with varying combinations on growth, survival and fatty acids profile of Macrobrachium rosenbergii larvae through Moina micrura enrichment

A study was carried out to determine the effect of tocopherol acetate along with cod liver oil astaxanthin enriched Moina micrura (MC- control, Ml- tocopherol acetate enriched, M2-tocopherol acetate combined with cod liver oil (CLO) enriched and M3- tocopherol acetate combined with astaxanthin enriched) on growth, survival and fatty acid composition of M. rosenbergii (de Man) larvae (TC- unenriched Moina fed larvae, Tl- tocopherol acetate enriched Moina fed larvae, T2- tocopherol acetate + CLO enriched Moina fed larvae to T3 – tocopherol acetate+ astaxanthin enriched Moina fed larvae). Growth was expressed as the time taken in to the settlement of 95% post larvae. Maximum growth i.e., the lowest time taken to the 95% PL settlement (40 days) and the maximum survival percentage (61%) was observed in both T2 and T3 treatments fed with M2 and M3 Moina respectively. Minimum growth and survival was observed in unenriched Moina fed larvae (TC). In larval treatments T2, (larvae fed with (M2) vitamin E + CLO enriched Moina), showed a higher percentage of EPA, DHA and higher HUFA level than other treatments.

Nutritive value of the crab Podophthalmus vigil (Fabricius)

Among the edible crabs, Podophthalmus vigil occupies a distinct position in Porto Novo coast. Though the fishery is seasonal, P. vigil supports an additional crab fishery from august to october. An attempt has been made to evaluate the nutritional status of different size groups of P. vigil. It has been observed that protein and moisture values are more in smaller crabs, corresponding with a drop in carbohydrate and fat. In bigger size groups, the values of fat and carbohydrate are found to be higher, while protein and moisture contents decreased slightly. The total meat content varies in different crabs of the same size group and the average value of the total consumable part is found to be only less than 30% of the total weight of the animal.

Technological aspects of preservation and processing of edible shell fish 2. Influence of season on the chemical composition of crab (Scylla serrata)

The purpose of this communication is to bring out the influence of season on the chemical composition of crab, covering a period of 2 years. Changes in moisture, protein, water extractable nitrogen, non-protein nitrogen, glycogen, lactic acid, fat and free amino acid composition of crab meat have been reported on a monthly basis.

Technological aspects of preservation and processing of edible shell fishes III. Factors influencing the keeping quality of crab (Scylla serrata) during freezing and frozen storage

The possible factors leading to the loss of flavour and general quality of crab during freezing and frozen storage have been studied. The preprocess ice storage condition of the raw material was found to be one such important factor while the fresh frozen crab meat remained in good organoleptic condition for about 51 weeks at -23°C, the 7 days iced material held frozen was found to have a shelf life of about 21 weeks. The fall in myofibrillar protein noted during frozen storage together with the loss of myosin ATPase activity correlated well with the loss of organoleptic qualities.

Technological aspects of preservation and processing of edible shell fishes IV. Comparative efficiency of different glazes in the preservation of frozen crab meat

The present work was undertaken to enlighten upon the comparative efficiency of different glazes in improving the quality of frozen crab meat (Scylla Serrata).

Studies on blue discolouration in canned body meat of crab (Scylla serrata)

Blue discolouration met with in the canned body meat of crab (Scylla serrata) was due to copper content exceeding 1.8 to 2.0 mg/100g on dry weight basis. Bleeding the cleaned carcasses of crab thoroughly in running water brought down the copper content below this level and blue discolouration prevented. Addition of copper ions to the thoroughly bled crab meat to raise the copper content above this level caused discolouration. The incidence of blue discolouration was independent of the freshness of the animals used. Citric acid in high concentration prevented blueing to some extent, but is not advisable as texture and flavour were adversely affected.

Morphometric measurements as an index for estimating yield of meat of shell fishes, 1. Crab (Scylla serrata) Forskal

Sixty one observations on length-breadth and whole weight-meat weight relations of India crab (Scylla serrata) were made. From the length of crab (cm) the whole weight (gm) can be computed by the equation: log W=-0.1708+2.3341 log L. Similarly for any given length (cm) the meat weight (gm) can be found by the relation, log w=-1.5745+3.0148 log L.

A suctorean parasite of Penaeus monodon larvae

A pathogenic suctorean, identified as Ephelota gemmipara was observed in P. monodon larvae spawned and reared in tanks. Commonly found to inhabit hydroid colonies, E. gemmipara has a stalked body with two types of tentacles, the sucking and piercing types, and was observed to reproduce by multiple exogenous budding.

Tolerance of Penaeus monodon larvae to cupric sulfate added in bath

Copper is used to deter the growth of bacterial, fungal and protozoan disease organism in fishes. Zoeae (Z SUB-1 ), myses (M SUB-1 ) and postlarvae (P SUB-1 ) were exposed to copper sulfate at concentrations of 0 . 025, 0 . 05, 0 . 75, 0 . 1 and 0 . 2 ppm from 24 to 96 hours. The number of surviving larvae were counted at the end of each 24-hour period and the percentage of survival is determined for each dose level. The LC SUB-50 for each of the larval stages was interpolated from the data whenever possible. Three trials with 2 replicates per trial were conducted. The physico-chemical characteristics of the bath taken before and at the end of the experimental period show insignificant differences between initial and final values in each trial. Results indicate that mortality rates of all larval stages increased with exposure time and that mortality rates of the experimental group is higher than the control. Interpolation of the LC SUB-50 is possible only for the 48-h and 72-h exposure times for both zoeae and myses and for the 48-h exposure time for the postlarvae. This is due to the high survival percentage of the 24-h group and the low survival percentage (below 50%) of the larvae exposed for 96 hours. The 48-hour LC SUB-50 for Z SUB-1 , M SUB-1 and P SUB-1 are 0 . 225, 0 . 350 and 0 . 125 ppm respectively. Postlarvae seem to be more sensitive than either of the 2 larval stages having a lower 48-h LC SUB-50 and a low survival rate after 72 hours. The larvae were observed to lose their balance and were lethargic, producing few swimming movements so that they were mostly confined to the bottom of the aquaria. Moribund larvae observed under the microscope had a faster but weak heartbeat compared to healthy larvae. Slight or complete loss of feeding ability indicated by empty guts and delayed molting of Z SUB-1 to Z SUB-2 were also noted.

Biology and farming of the green mussel Mytilus smaragdinus

Biological investigations were carried out in Sapian Bay, Capiz from November 1975 to December 1976 with samplings conducted fortnightly. Histological studies on the gonad reveal a high percentage of ripe and spent females during the month of April and May, and ripe to near ripe during November to December. However, larval counts were highest on February 25, 1976 with 253 mytilid larvae per haul compared to 0-79 per haul during all other months. The high larval count was followed by the highest spat settlement during the next sampling period two weeks later, with the spat collector set in the water during the February 25 sampling. The four materials tested, blue polypropylene fiber rope, black polypropylene fiber, and coir rope, all had their highest spat counts during this period with an average of 471 spats per standard 10 cm rope piece. The range during the other time periods is 2-283 spats. Of the 4 materials tested, the black fibrillated polypropylene film had the highest larval counts in 15 out of a total of 25 sampling periods. The blue rope was the poorest spat collector. Coconut husk was tested later on and it proved to have a very high catchability, with spats completely enveloping the husk surface. Growth monitored from one cohort in Sapian Bay averaged 10 mm per month. 50-60 mm is considered marketable size. Trial growth experiments with transplanted mussels were also conducted at Igang Bay in Guimaras Island, Makato River in Aklan, and a milkfish pond in Leganes, Iloilo. Survival in Igang was less than 50% after the second week, and the condition of the surviving mussels can be described only as 'watery' with the mantle completely transparent. Mortality was minimal in Makato but the growth rate was only 30% that of Sapian Bay. The pond experiments were terminated due to severe crab predation.

Use of fermented kitchen waste in rearing Penaeus monodon larvae

Fermented vegetable and kitchen wastes are available as feeds for not only zoea but also mysis and up to certain points in the postlarval stages of sugpo, Penaeus monodon. It is recommended that the hatchery use fermented wastes as larval feed for P.monodon when diatoms or brine shrimp nauplii are lacking or in short supply. Among three stages namely, zoea, mysis and postlarva, the survival rate during postlarva particularly after P SUB-4 was quite low. The problems encountered are as follows: (a) how to prevent fermented particles from lumping, (b) how to prevent them from easily sinking to the bottom, and, (c) how to prevent bacteria and fungi, particularly Lagenidium sp blooming.