Ocorrência de Listeria spp. e de Listeria monocytogenes, em um matadouro-frigorífico de bovinos do estado de São Paulo

We evaluated the presence of Listeria spp. and Listeria monocytogenes in environmental samples by means of swabs collected the bovine slaughter plant enabled to export, located in the state of Sao Paulo, Brazil. After the pre-enrichment at 30±1°C for 22 to 26h the samples were analyzed using the BAX System Listeria. Those positive for Listeria spp. were submitted a second PCR reaction to confirm the presence of Listeria monocytogenes. From 411 environmental samples analyzed, 62 (15.1%) were positive for Listeria spp. and 21 (5.1%) for Listeria monocytogenes, which showed their persistence in the slaughter plant. There were no statistical differences between the rainy and dry periods and between areas sampled, although it has been found between sectors. The floor surface and the sector cuts have higher rates of positivity.

Atividade antimicrobiana in vitro de extrato de Punica granatum L. sobre staphylococcus aureus isolado em leite bovino

Bovine mastitis is considered important disease causing major economic losses in dairy herds. Antimicrobial drug can promote resistance, chemical residues in food and environmental contamination. The purpose of this study was to evaluate the antibacterial activity in vitro of pomegranate extract on Staphylococcus aureus isolated from bovine milk, evaluate its antioxidant activity, and quantify levels of total phenols and fravonoides of the different extracts used. Aqueous extracts were used in nature and dry, from the peel of the fruit (EAC) and leaves (EAF). Additionally, it was evaluated the antioxidant activity (AA%), total phenols and flavonoids. Milk samples were inoculated, incubated, and the colonies were characterized as Staphylococcus aureus were adjusted to 1.0x106 UFC/mL in the 6 standard of the MacFarland scale. The sensitivity of the microbial isolates were determined in quintuplicate, in disk diffusion test. The minimum inhibitory concentration was determined by visible inhibition zone greater than 15mm. The results were evaluated by ANOVA, Tukey 5%, using the program SISVAR 5.3 - DEX/UFLA. Results implied that the aqueous extract from the bark of dried fruit was capable of inhibiting bacterial growth at concentrations of 3%. The other treatments only showed this activity, from the concentrations of 15%, 20% and 30% for dry EAF, in nature EAC and in nature EAF, respectively. Regarding the action antioxidant of dry EAC, was not correlated with total phenols and flavonoids. Probably other alkaloids substances present in the extract studied, may have been responsible for this activity. It is concluded that extracts of Punica granatum L., especially those obtained by the shell of the fruit, showed inhibitory activity against S. aureus, indicating your use potential for the control of bovine mastitis.

Recent mouse and rat methods for the study of experimental oral candidiasis

The Candida genus expresses virulence factors that, when combined with immunosuppression and other risk factors, can cause different manifestations of oral candidiasis. The treatment of mucosal infections caused by Candida and the elucidation of the disease process have proven challenging. Therefore, the study of experimentally induced oral candidiasis in rats and mice is useful to clarify the etiopathology of this condition, improve diagnosis, and search for new therapeutic options because the disease process in these animals is similar to that of human candidiasis lesions. Here, we describe and discuss new studies involving rat and mouse models of oral candidiasis with respect to methods for inducing experimental infection, methods for evaluating the development of experimental candidiasis, and new treatment strategies for oral candidiasis. © 2013 Landes Bioscience.

Períodos de interferência de plantas daninhas na cultura do girassol

The interference of weeds in sunflower cultivation can significantly reduce the productivity of achenes. The objective of this study was to determine the period before interference and total period of interference of the weed community on productivity and achene oil of sunflower cultivation. The experiment was conducted under field conditions in the fieldin Botucatu (SP) in the 2007/2008 agricultural year. The experimental design was a randomized complete block with four replications. The treatments were composed of portions represent periods of control and presence of weeds. For control periods, the culture was kept free of weeds by increasing periods of 7, 14, 21, 28, 35, 42, 49 and 110 days after the emergence (DAE) of sunflower. For periods of coexistence, the culture was maintained in the presence of the weed community for the same periods. We assessed the following variables: diameter of chapters, achene yield, oil yield of sunflower, density and dry matter of weeds and phytosociological indices. The period before the interference was 35 DAE culture to the productivity of achenes, and the total period of interference lasted until 24 DAE. For oil yield, the period before the interference was 25 DAE, whereas the total period of interference lasted for 14 DAE.

Respostas fisiológicas de cultivares de cana-de-açúcar a herbicidas seletivos

The use of selective herbicides to control weeds has caused different responses in cultivars of sugar cane, and some products affect physiological characteristics and reduce the photosynthetic activity. This study aimed to evaluate the physiological traits in cultivars of sugar cane under the effect of applying post-emergence herbicides. The test was developed in Jau, SP. The experimental design was randomized blocks in factorial scheme 5 x 4 (cultivar x herbicide) with four replications. SP81-3250, RB855156, RB855453, RB867515, IACSP95-5000 were grown in this studied. Herbicides clomazone (1200 g i.a.ha-1); commercial mixture of clomazone + ametryn (1000 + 1500 g i.a.ha-1) and ametryn (3000 g i.a.ha-1) and a control were applied at 30 days after planting. Cultivars IACSP95-5000 and RB867515 were less affected physiologically and can be considered selective to these herbicides. The application of clomazone and ametryn affected negatively the traits maximum photochemical efficiency of photosystem II (Fv/Fm), SPAD index and photosynthetic pigments, but the mixture of these herbicides caused higher reductions, indicating to be the more aggressive to the cultivars.

Estimativa da área e capacidade de retenção foliar de calda em citros

This work was done to determine the maximum amount of liquid that the citrus leaves can hold back and compare alternative methods for estimating leaf area with the standard method of integrating electronic image. The alternative methods were leaf mirroring on paper and leaf digitalization and imagine analyzes. The spray retention capacity was evaluated with mitecide sprayed with cyhexatin (Sipcatin 500 CS) plus the treatments: combination of two adjuvants (mineral oil - Assist and vegetable oil - Veget'Oil) and two concentrations (10 and 15 mL of adjuvant L-1). The methods for estimating leaf area assessed do not differ between them. The maximum retention of liquids for the leaf occurred when vegetable oil in the application was used.

Desempenho produtivo e qualidade pós-colheita de genótipos de feijão do grupo comercial carioca cultivados na época de inverno-primavera

The objective of this work was to evaluate the agronomic performance and grain quality, especially the grain technological characteristics in the common bean genotypes, cultivated in winter-spring season. The experiment was carried out in Jaboticabal-SP, in a Rhodic Hapludox. The experimental design was a randomized block with 17 treatments (genotypes) with three replications. The plots had been composed for 17 carioca common bean group genotypes (Pérola, BRS Cometa, BRS Pontal, IPR Juriti, CNFC 10703, CNFC 10713, CNFC 10716, CNFC 10721, CNFC 10729, CNFC 10733, CNFC 10742, CNFC 10753, CNFC 10757, CNFC 10758, CNFC 10762, CNFC 10763 and CNFC 10813). BRS Pontal and CNFC 10716 showed simultaneously the higher number of per plant and grain yield. The technological characteristics more expressive was verified in CNFC 10703, CNFC 10713, CNFC 10758, CNFC 10813 and CNFC 10716 genotypes, principally about the protein content, cooking time and grain hydration capacity. The CNFC 10716 genotype detached as promissory, because their elevated agronomic performance and satisfactory technological characteristics.

Parâmetros genéticos entre caracteres quantitativos no girassol como critério de seleção para produtividade de aquênios

Two experiments were conducted in southern Brazil in no-tillage system, in order to estimate genetic parameters and the direct and indirect effects of components for achene yield as a selection criterion in sunflower. We analyzed eight sunflower hybrids at two locations, in a randomized complete block design with four replicates, determined through quantitative descriptors: insertion height of the head, and head stem diameter, weight of 1000 achenes, number of achenes per head, mass by achene head and yield achene. Estimates of genetic parameters were based on combined analysis, decomposing interactions in genetic and environmental components. Considering the coefficient of genetic variation, indirect effects of components and heritability, there are higher possibilities for responses to selection in sunflower achenes by descriptors mass and mass of achenes per head, with its indirect association interrelated pathways for the increase in the achenes of yield.

Produção de biomassa, rendimento e composição do óleo essencial de manjericão (Ocimum basilicum L.) em função de reguladores vegetais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Acompanhamento clínico-laboratorial de gatos naturalmente infectados com vírus da imunodeficiência (FIV) tratados com zidovudina(AZT)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Níveis de potássio em solução nutritiva, desenvolvimento de plantas e a produção de óleo essencial de mentha piperita L

Pós-graduação em Agronomia (Horticultura) - FCA

Desenvolvimento, produção e composição de óleo essencial da Mentha piperita L., cultivada em solução nutritiva com diferentes níveis de nitrogênio

Pós-graduação em Agronomia (Horticultura) - FCA

Efeitos de auxinas e boro no enraizamento de estacas caulinares de louro (Laurus nobilis L.)

Pós-graduação em Agronomia (Horticultura) - FCA

Efeito agudo dos exercícios com pesos sobre os níveis de leptina, adiponectina e fator de necrose tumoral alfa em adultos não treinados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeitos de reguladores vegetais no desenvolvimento de plantas de soja(Glycine Max(L.)Merrill)

Pós-graduação em Ciências Biológicas (Botânica) - IBB