Tuna : competitive conditions affecting the U.S. and European tuna industries in domestic and foreign markets : report to the Committee on Finance, U.S. Senate, and the Committee on Ways and Means, U.S. House of Representatives, investigation no. 332-291 under section 332 of the Tariff Act of 1930

Mode of access: Internet.

Durum wheat : conditions of competition between the U.S. and Canadian industries : report on investigation No. 332-285 under Section 332(g) of the Tariff Act of 1930 as amended.

"June 1990."

A botanical survey of U.S. route 45 [FAP 332, section 31-1, 42-1], from Harrisburg to Eldorado [from IL route 13 to IL route 142], Saline County, Illinois, with emphasis on threatened and endangered plant species and natural areas /

Cover title.

A History of the Church in five books, from A.D.332 to the death of Theodore of Mopsuestia A.D.427 /

Mode of access: Internet.

Operator's, organizational and direct support, maintenance manual including repair parts and special tool list : pump, centrifual [sic], pneumatic, sump, 210 GPM (Ingersoll-Rand model 251), FSN 4320-021-1351.

Includes index.

Organizational, DS, GS, and depot maintenance manual : lubricating and servicing unit, trailer mounted; 16 CMF compressor, gasoline driven, (Gray Company, Inc. mode1 251-437), FSN 4930-892-5067.

"February 1965."

Organizational maintenance repair parts and special tools lists : lubricating and servicing unit, trailer mounted, 15 CMF compressor, gasoline driven, (Gray model 251-437), FSN 4930-892-5067.

"November 1968."

Direct and general support and depot maintenance repair parts and special tools lists : lubricating and servicing unit, trailer mounted, 15 cfm compressor, gasoline driven (Gray model 251-437), FSN 4930-892-5067.

"November 1968."

Barometers, ML-330/FM, ML-331/TM, ML-332/TM, ML-333/TM.

"27 November 1945."

Operator's, organizational, direct support, and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and sepcial [sic] tools) : masts AB-155/U (FSN 5820-251-2366), AB-155A/U (FSN 5985-507-6261), and AB-155B/U (FSN 5985-732-5146).RC-74 and CY-6314A/PRC-74 (NSN 5820-00-156-3934).

Includes index.

Little Butte/Bear Creek Subbasins Water Feasibility Act : report (to accompany S. 251).

"October 27, 2005."

An evaluation of the training provided in correctional institutions under the Manpower development and training act, section 251; final report.

Report MEL 71-09a-c.

Kratke dejiny a seznam, cesko=katolickych osad ve spoji statech Americkych : ku vzpomince na 251 ete jubilium veledustp farare.

Mode of access: Internet.

Tailored laminin-332 alpha3 sequence is tethered through an enzymatic linker to a collagen scaffold to promote cellular adhesion

Surface modification techniques have been used to develop biomimetic scaffolds by incorporating cell adhesion peptides, which facilitates cell adhesion, migration and proliferation. In this study, we evaluated the cell adhesion properties of a tailored laminin-332 alpha3 chain tethered to a type I collagen scaffold using microbial transglutaminase (mTGase) by incorporating transglutaminase substrate peptide sequences containing either glutamine (peptide A: PPFLMLLKGSTREAQQIVM) or lysine (peptide B: PPFLMLLKGSTRKKKKG). The degree of cross-linking was studied by amino acid analysis following proteolytic digestion and the structural changes in the modified scaffold further investigated using Fourier transform infrared spectroscopy and atomic force microscopy. Fibroblasts were used to evaluate the cellular behaviour of the functionalized collagen scaffold. mTGase supports cell growth but tethering of peptide A and peptide B to the mTGase cross-linked collagen scaffold caused a significant increase in cell proliferation when compared with native and mTGase cross-linked collagen scaffolds. Both peptides enabled cell-spreading, attachment and normal actin cytoskeleton organization with slight increase in the cell proliferation was observed in peptide A when compared with the peptide B and mTGase cross-linked scaffold. An increase in the amount of epsilon(gamma-glutamyl) lysine isopeptide was observed in peptide A conjugated scaffolds when compared with peptide B conjugated scaffolds, mTGase cross-linked scaffold without peptide. Changes in D-spacing were observed in the cross-linked scaffolds with tethered peptides. These results demonstrate that mTGase can play a bifunctional role in both conjugation of the glutamine and lysine containing peptide sequences and also in the cross-linking of the collagen scaffold, thus providing a suitable substrate for cell growth.