À procura da identidade de Heinz : um modelo de compreensão da motivação moral

RESUMO: As teorias psicológicas da moralidade dividem-se na conceptualização da motivação moral, o tema desta dissertação. Os modelos construtivistas, apoiados na epistemologia genética, privilegiam a cognição como factor determinante mas, as teorias que enfatizam o funcionamento individual admitem que as emoções e a identidade interferem na tendência de cada pessoa agir moralmente. Particularmente apoiados na segunda perspectiva, testámos um modelo preditor da motivação moral com três variáveis, identidade, identidade moral e integridade, todas avaliadas por medidas psicométricas, em duas amostras. A primeira era composta por 320 estudantes do 12º ano de uma escola do concelho de Lisboa, com idades compreendidas entre 18 e 20 anos (M=18,22; Dp=0,49), 108 masculinos e 194 femininos e a segunda era composta por 174 sujeitos, estudantes finalistas de cursos profissionais de uma escola do concelho de Lisboa frequentada por alunos provenientes de ambientes sociais caracterizados pelo stresse sócio-económico e familiar, e até, em bastantes casos, que já tinham participado em actos ilegais relacionados com furto, vandalismo, tráfico de drogas, e agressões, com idades compreendidas entre 18 e 22 anos (M=18,98; Dp=1,075), 104 masculinos e 70 femininos. A primeira amostra foi considerada normativa e a segunda de risco psicossocial. Para avaliar a motivação moral foi construída uma medida que incluiu dois aspectos: a consciência moral, relativa à avaliação objectiva e subjectiva que os indivíduos fazem das situações morais que através de processos de análise de conteúdo foi distinguida em três categorias exclusivas: não transgressão, transgressão relativizada e transgressão; e a autoatribuição de emoções que indica a probabilidade objectiva dos indivíduos cometerem acções morais. Foi verificada a existência de diferenças individuais em ambas as medidas da motivação moral e, também, a existência de diferenças na atribuição de emoções em função da consciência moral: os sujeitos da categoria transgressão apresentaram atribuições mais negativas do que os sujeitos de transgressão relativizada e estes, por sua vez, apresentaram atribuições mais negativas que os sujeitos da categoria não transgressão. Estes resultados confirmam o papel da identidade na motivação moral. A análise da predição confirmou que a integridade, a identidade e a identidade moral, em ordem decrescente de capacidade explicativa, constituem factores preditores tanto da consciência moral como da auto-atribuição de emoções. ABSTRACT: Moral motivation, the subject of this thesis, is differently viewed by several psychological perspectives. Constructivist models supported by genetic epistemology define cognition as the determinant factor while theories that emphasize individual functioning admit that emotions and identity have a central role in the way that persons could morally act. Particularly supported in this second approach we tested a predictive model of moral motivation with three variables, identity, moral identity and integrity, all evaluated by psychometric scales, in two samples. The first sample had 320 graduate high school students with age between 18 and 20 (M=18,22; SD=0,49), 108 male and 194 female and the second sample had 174 graduate students of vocational courses in high school of a risk social and familiar environment, many of them had already done illegal acts such as stealing, vandalism, drug traffic and aggressions; they had ages between 18 and 22 (M=18,98; SD=1,08), 104 male and 70 female. The first sample was considered normative and the second one was classified as psychosocial risk. To evaluate moral motivation we developed a measure that assesses two aspects: moral conscience, who includes both objective and subjective evaluation of moral situations that was distinguished in three exclusive categories by procedures of content analysis: non-transgression, justifiable transgression and transgression; and self-attribution of emotion that indicates the likelihood of committing moral actions. We observed individual differences in both measurements of moral motivation and also verified differences in emotional attribution on the basis of moral conscience: the individuals of transgression category presented more negative attributions than those of justifiable transgression and in turn the latter presented more negative attributions than individuals of non-transgression category. These results confirm the role of identity in moral motivation. The predictor analysis confirmed integrity, identity and moral identity, in descending order of explanatory power, as predictive variables of moral consciousness and self-attribution of emotions.

La prescripción tributaria: estudio comparativo Ecuador - países andinos

La prescripción es una figura del derecho que al aplicarse a lo tributario conlleva dificultades y cuestionables interpretaciones. Por otra parte, la doctrina, al no tener una posición definida sobre la naturaleza de la prescripción, genera discrepancias entre los estudiosos del derecho. Esta investigación pretende contribuir al debate sobre el asunto, analizando el tema de la prescripción tributaria en el Ecuador y en los países de la Comunidad Andina. Se inicia con el estudio de la prescripción tributaria: cuándo nace, desde cuándo es exigible, cuál es su naturaleza, su relación con la prescripción del derecho civil y sus diferencias con la caducidad. Además, aborda la interrupción de la prescripción tributaria, indagado tanto sus causas y efectos, por qué la prescripción se interrumpe y no se suspende, si el tiempo contabilizado para que opere la prescripción debe ser concebido como término o plazo, para enseguida analizar los efectos qué produce la prescripción con respecto a los sujetos de la relación jurídica tributaria. Finalmente, se realiza un estudio comparativo con las correspondientes legislaciones de Colombia, Perú, Bolivia y Venezuela, y se establecen ciertas recomendaciones que, a criterio de la autora, permitirán una mejor y más fácil aplicación de la normas del derecho tributario relacionadas con la prescripción.

Interpretación y aplicación de la sentencia C-577 de 2011 frente al matrimonio entre personas del mismo sexo en Colombia

En Colombia el reconocimiento de los derechos de las personas Lesbianas, Gays, Bisexuales, Transgenero e Intergenero (LGBTI) encuentra su punto de partida en la Constitución Política de 1991. Durante los primeros años de su vigencia hasta el 2007, la Corte Constitucional como órgano encargado de su interpretación, definió los derechos individuales de las personas LGBTI a partir de principios como la dignidad humana, el libre desarrollo de la personalidad, la igualdad, la prohibición de discriminar y el pluralismo. Con la expedición de la sentencia C-075 de 2007 inicia una nueva fase en la movilización de esta comunidad; por primera vez se le reconocen efectos jurídicos a las uniones conformadas por personas del mismo sexo, declarando sus derechos en la esfera de pareja, pero limitándolos al negar su estatus de familia. En el año 2011 el estudio de las parejas de personas de igual sexo dentro del ordenamiento jurídico colombiano cambia radicalmente. Mediante la sentencia C-577 de 2011, el alto tribunal declaró que estas uniones constituyen una forma de familia y por lo tanto tienen derecho a formalizar su vínculo mediante una figura jurídica de orden contractual que les brinde un margen de protección más amplio que el de la unión marital de hecho. El acceso al matrimonio civil igualitario por parte de las parejas integradas por personas del mismo sexo constituye actualmente uno de los temas más debatidos en Colombia. La discusión es significativa porque confronta el modelo tradicional de familia, pareja y matrimonio asociado con la heterosexualidad y la reproducción; adicionalmente evidencia los límites que configuran la noción de lo humano, del sujeto de derechos y de la igualdad como principio condicionado a cualidades de orden mayoritario.

Modelling impacts of pollution in river systems: a new dispersion model and a case study of mine discharges in the Abrud, Aries and Mures river system in Transylvania, Romania

A new model of dispersion has been developed to simulate the impact of pollutant discharges on river systems. The model accounts for the main dispersion processes operating in rivers as well as the dilution from incoming tributaries and first-order kinetic decay processes. The model is dynamic and simulates the hourly behaviour of river flow and pollutants along river systems. The model has been applied to the Aries and Mures River System in Romania and has been used to assess the impacts of potential dam releases from the Roia Montan Mine in Transylvania, Romania. The question of mine water release is investigated under a range of scenarios. The impacts on pollution levels downstream at key sites and at the border with Hungary are investigated.

Exchangeability of mammalian DNA ligases between base excision repair pathways

In mammalian cells, DNA ligase IIIalpha and DNA ligase I participate in the short- and long-patch base excision repair pathways, respectively. Using an in vitro repair assay employing DNA ligase-depleted cell extracts and DNA substrates containing a single lesion repaired either through short-patch (regular abasic site) or long-patch (reduced abasic site) base excision repair pathways, we addressed the question whether DNA ligases are specific to each pathway or if they are exchangeable. We find that immunodepletion of DNA ligase I did not affect the short-patch repair pathway but blocked long-patch repair, suggesting that DNA ligase IIIa is not able to substitute DNA ligase I during long-patch repair. In contrast, immunodepletion of DNA ligase IIIa did not significantly affect either pathway. Moreover, repair of normal abasic sites in wild-type and X-ray cross-complementing gene 1 (XRCC1)-DNA ligase IIIalpha-immunodepleted cell extracts involved similar proportions of short- and long-patch repair events. This suggests that DNA ligase I was able to efficiently substitute the XRCC1-DNA ligase IIIa complex during short-patch repair.

The gut microbiota in obesity and metabolic disease - a novel therapeutic target

Obesity is sweeping the westernized world at a rate which far outstrips human genomic evolution, highlighting the importance of the obesogenic environment. Diet is an important component of this obesogenic environment, with certain diets (high fat, high refined carbohydrates and sugar) predisposing to overweight. On the other hand, there are also foods shown to protect against obesity and the diseases of obesity, including whole plant foods, dairy products, dietary fibre and functional foods like probiotics, prebiotics and phytochemicals. Interestingly, many of these foods mediate their health-promoting activities through the gut microbiota. The human gut microbiota itself has recently been identified as a contributory factor in this obesogenic environment, with differences observed between lean and obese. Evidence from human studies indicates that important groups of fermentative bacteria differ in abundance between lean and obese. Recently it has been suggested that anomalous microbiota composition in infancy can predispose to overweight in later life, highlighting the important role of optimal microbiota successional development, and that – as observed in laboratory animals – the gut microbiota may contribute to the aetiology of obesity. In this review we will introduce the gut microbiota, describe its interactions with major dietary components and the host, and then go on to discuss evidence indicating that the gut microbiota may contribute to the obesogenic environment. Finally, we will explore possible strategies for modulating the composition and activity of the human gut microbiota which may impact on obesity or the metabolic diseases associated with obesity. (Nutritional Therapy & Metabolism 2009; 27: 113-33)

Emulsifying, rheological and physicochemical properties of exopolysaccharide produced by Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205

The rheological, emulsification and certain physicochemical properties of purified exopolysaccharides (EPS) of Bifidobacterium longum subsp. infantis CCUG 52486 and Bifidobacterium infantis NCIMB 702205 were studied and compared with those of guar gum and xanthan gum. The two strains were grown in skim milk supplemented with 1.5% (w/v) casein hydrolysate at 37 ◦C for 24 h; they both produced heteropolysaccharides with different molecular mass and composition. The carbohydrate content of both polymers was more than 92% and no protein was detected. The EPS of B. longum subsp. infantis CCUG 52486 showed highly branched entangled porous structure under scanning electron microscopy. Higher intrinsic viscosity was observed for the EPS of B. longum subsp. infantis CCUG 52486 compared to the EPS of B. infantis NCIMB 702205 and guar gum. Both polymers showed pseudoplastic non-Newtonian fluid behaviour in an aqueous solution. The EPS of B. infantis NCIMB 702205 and B. longum subsp. infantis CCUG 52486 produced more stable emulsions with orange oil, sunflower seed oil, coconut oil and xylene compared to guar and xanthan gum. The EPS of B. longum subsp. infantis CCUG 52486 is the most promising one for applications in the food industry, as it had higher intrinsic viscosity, higher apparent viscosity in aqueous solution, porous dense entangled structure and good emulsification activity.

Investigation of the impact of feeding Lactobacillus plantarum CRL 1815 encapsulated in microbially derived polymers on the rat faecal microbiota

AIMS: The aim of this study was to evaluate the impact of the administration of microencapsulated Lactobacillus plantarum CRL 1815 with two combinations of microbially derived polysaccharides, xanthan : gellan gum (1%:0·75%) and jamilan : gellan gum (1%:1%), on the rat faecal microbiota. METHODS AND RESULTS: A 10-day feeding study was performed for each polymer combination in groups of 16 rats fed either with placebo capsules, free or encapsulated Lact. plantarum or water. The composition of the faecal microbiota was analysed by fluorescence in situ hybridization and temporal temperature gradient gel electrophoresis. Degradation of placebo capsules was detected, with increased levels of polysaccharide-degrading bacteria. Xanthan : gellan gum capsules were shown to reduce the Bifidobacterium population and increase the Clostridium histolyticum group levels, but not jamilan : gellan gum capsules. Only after administration of jamilan : gellan gum-probiotic capsules was detected a significant increase in Lactobacillus-Enterococcus group levels compared to controls (capsules and probiotic) as well as two bands were identified as Lact. plantarum in two profiles of ileum samples. CONCLUSIONS: Exopolysaccharides constitute an interesting approach for colon-targeted delivery of probiotics, where jamilan : gellan gum capsules present better biocompatibility and promising results as a probiotic carrier. SIGNIFICANCE AND IMPACT OF STUDY: This study introduces and highlights the importance of biological compatibility in the encapsulating material election, as they can modulate the gut microbiota by themselves, and the use of bacterial exopolysaccharides as a powerful source of new targeted-delivery coating material.

Preparation and magnetic properties of barium ferrites substituted with manganese, cobalt, and tin

Barium ferrites substituted by Mn–Sn, Co–Sn, and Mn–Co–Sn with general formulae BaFe12−2xMnxSnxO19 (x=0.2–1.0), BaFe12−2xCoxSnxO19 (x=0.2–0.8), and BaFe12−2xCox/2Mnx/2SnxO19 (x=0.1–0.6), respectively, have been prepared by a previously reported co-precipitation method. The efficiency of the method was refined by lowering the reaction temperature and shortening the required reaction time, due to which crystallinity improved and the value of saturated magnetization increased as well. Low coercivity temperature coefficients, which are adjustable by doping, were achieved by Mn–Sn and Mn–Co–Sn doping. Synthesis efficiency and the effect of doping are discussed taking into account accumulated data concerning the synthesis and crystal structure of ferrites.

Effect of high-hydrostatic pressure and pH treatments on the emulsification properties of gum arabic

This study investigated the emulsification properties of the native gums and those treated at high pressure (800 MPa) both at their “natural” pH (4.49 and 4.58, respectively) and under “acidic and basic” pH (2.8 and 8.0) conditions. The emulsification behaviour of KLTA gum was found to be superior to that of the GCA gum. High pressure and pH treatment changed the emulsification properties of both gums. The acidic amino acids in gum arabic were shown to play an important role in their emulsification behaviour, and mechanisms of emulsification for the two gums were suggested to be different. The highly “branched” nature of the carbohydrate in GCA gum was also thought to be responsible for the “spreading” of droplet size distributions observed. Coomassie brilliant blue binding was used to indicate conformational changes in protein structure and Ellman’s assay was used to estimate any changes in levels of free thiols present.

Carotenoids but not flavonoids are associated with improvements in spatial working memory in younger adults in a flavonoid-rich v. -poor fruit and vegetable intervention study

Findings from animal studies suggest that components of fruit and vegetables (F&V) may protect against, and even reverse, age-related decline(1,2) in aspects of cognitive functioning such as spatial working memory (SWM). Human subjects in vivo and in vitro studies indicate that anti-inflammatory, anti-oxidant and cell-signalling properties of flavonoids and carotenoids, non-nutrient components of F&V, may underpin this protective effect(3–5). The Flavonoid University of Reading Study (FLAVURS), designed to explore the dose-response relationship between dietary F&V flavonoids and CVD, enabled the investigation of such an association with SWM. FLAVURS is an 18-week parallel three-arm randomised controlled dietary intervention trial with four time points, measured at 6-weekly intervals from baseline. Low F&V consumers at risk of CVD aged 26–70 years were randomly assigned to high flavonoid (HF), low flavonoid (LF) or control group. F&V intake increased by two daily 80 g portions every 6 weeks, with either HF or LF F&V, in addition to each participant's habitual diet, while controls maintained their habitual diet. At each visit, participants completed a cognitive test battery with SWM as the primary outcome. The HF group showed significantly higher levels of urinary flavonoids than LF or controls at 12 weeks (P<0.001) as expected, but surprisingly only higher levels than LF at 18 weeks (P<0.01). The LF group showed higher levels of plasma carotenoids than the other groups at 18 weeks (P<0.001). No group differences were found for SWM overall, however, age-group sub-analyses (26–50 and 51–70 years of age) showed differences from 0 to 18 weeks for younger adults, with LF improving significantly more than the other two groups on SWM (P<0.05). As nutritional absorption is known to decrease with age, separate stepwise regressions were performed on the two age groups irrespective of dietary group, with urinary flavonoids and plasma carotenoids as predictors. For younger adults, improved SWM performance from 0 to 18 weeks was associated with higher carotenoid levels, β=0.28, t(55)=2.10, P<0.05, accounting for 7.5% of the variance, R2=0.075, F(1,54)=4.41, P=0.040. For older adults, no between-group SWM differences were found. Findings suggest that F&V-based flavonoids and carotenoids may provide benefits for cognitive function, and that carotenoids in particular may improve cognitive performance in SWM. Given that these benefits were restricted to younger adults, future work is needed to test the reliability of this finding, as well as determine the mechanisms by which age-dependent differences in F&V responsiveness occur.

Thermoelectric properties of TiS2 mechanically alloyed compounds

Bulk polycrystalline samples in the series Ti1−xNbxS2 (0 ≤ x ≤ 0.075) were prepared using mechanical alloying synthesis and spark plasma sintering. X-ray diffraction analysis coupled with high resolution transmission electron microscopy indicates the formation of trigonal TiS2 by high energy ball-milling. The as-synthesized particles consist of pseudo-ordered TiS2 domains of around 20–50 nm, joined by bent atomic planes. This bottom-up approach leads, after spark plasma sintering, to homogeneous solid solutions, with a niobium solubility limit of x = 0.075. Microstructural observations evidence the formation of small crystallites in the bulk compounds with a high density of stacking faults. The large grain boundary concentration coupled with the presence of planar defects, leads to a substantial decrease in the thermal conductivity to 1.8 W/mK at 700 K. This enables the figure of merit to reach ZT = 0.3 at 700 K for x = 0.05, despite the lower electron mobility in mechanically alloyed samples due to small crystallite/grain size and structural defects.

Ras: the stress and the strain

The last 10–15 years have seen an expansion in the understanding of the intracellular signalling pathways activated in cardiac myocytes in response to hypertrophic or lethal stimuli. The mitogen-activated protein kinases (MAPKs) were identified as potential key mediators of cardiac myocyte responses in the early to mid-1990's, with the extracellular signal-regulated kinases 1/2 (ERK1/2) being potently activated by heterotrimeric Gq protein-coupled receptor (GqPCR) agonists, and the c-Jun N-terminal kinases (JNKs) and p38-MAPKs being potently activated by cell stresses.

Four novel mononuclear and polynuclear Cu(I) thiosaccharinates with triphenylphosphane and bis(diphenylposphino)methane. Synthesis and structural study of Cu(4)(tsac)(4)(PPh(3))(3), Cu(tsac)(PPh(3))(2), Cu(4)(tsac)(4)(dppm)(2), and Cu(2)(tsac)(2)(dppm)(2)

Four new ternary complexes of copper(I) with thiosaccharin and phosphanes were prepared. The reaction of [Cu(4)(tsac)(4)(CH(3)CN)(2)] (1) (tsac: thiosaccharinate anion) with PPh(3) in molar ratios Cu(I)/PPh(3) 1:075 and 1:2 gave the complexes [Cu(4)(tsac)(4)(PPh(3))(3)] center dot CH(3)CN (2) and Cu(tsac)(PPh(3))(2) (3), respectively. The reaction of 1 with Ph(2)PCH(2)PPh(2) (dppm) in molar ratios Cu(I)/dppm 2:1 and 1:1 gave the complexes [Cu(4) (tsac)(4)(dppm)(2)] center dot 2CH(2)Cl(2) (4) and [Cu(2)(tsac)(2)(dppm)(2)] center dot CH(2)Cl(2) (5), respectively. All the compounds have been characterized by spectroscopic and X-ray crystallographic methods. Complex 2 presents a tetra-nuclear arrangement with three metal centers in distorted tetrahedral S(2)NP environments, the fourth one with the Cu(I) ion in a distorted trigonal S(2)N coordination sphere, and the tsac anions acting as six electron donor ligands in mu(3)-S(2)N coordination forms. Complex 3 shows mononuclear molecular units with copper(I) in a distorted trigonal planar coordination sphere, built with the exocyclic S atom of a mono-coordinated thiosaccharinate anion and two P-atoms of triphenylphosphane molecules. With dppm as secondary ligand the structures of the complexes depends strongly on the stoicheometry of the preparation reaction. Complex 4 has a centrosymmetric structure. Two triply bridged Cu(2)(tsac)(2)(dppm) units are joined together by the exocyclic S-atoms of two tsac anions acting effectively as bridging tridentate ligands. Complex 5 is conformed by asymmetric dinuclear moieties where the two dppm and one tsac ligands bridge two Cu(I) atoms and the second tsac anion binds one of the metal centers through its exocyclic S-atom. (C) 2009 Elsevier B.V. All rights reserved.

Potential antitumoral properties of a new copper complex with santonic acid

A new copper(II) complex of santonic acid [Cu(2)(sant)(4)(H(2)O)(2)]center dot 21/2H(2)O has been prepared and characterized by electronic, vibrational, EPR spectral studies, and stability determinations in solution. The presence of two antiferrromagnetically coupled copper centers in the solid state was detected by EPR. The dinuclear Cu(II) complex crystallizes in the tetragonal P4(3)2(1)2 space group, with a = b = 14.498(3), c = 64.07(1) angstrom. Biological studies indicate that the complex displays interesting potential antitumoral actions. (C) 2008 Elsevier Ltd. All rights reserved.