Sequence analysis of the 3' hypoxia-responsive element of the human erythropoietin gene in patients with erythrocytosis

Erythrocytosis arises from a variety of pathogenic mechanisms. We sequenced a 256-bp region 3' to the erythropoietin (Epo) gene which included a 24- to 50-bp minimal hypoxia-responsive element spanning HIF-1- and HNF-4-binding sites in 12 patients with erythrocytosis and 4 normal subjects. Four polymorphisms were found, none of which affected the HIF-1-binding site, although one polymorphism was present in the HNF-4 consensus region. The data indicate that none of these polymorphisms cause erythrocytosis.

The sedimentology of the Bloomington fan complex: an element of the Oak Ridges Moraine, Southern Ontario

The Oak Ridges Moraine is a major physiographic feature of south-central Ontario, extending from Rice Lake westward to the Niagara Escarpment. While much previous work has largely postulated a relatively simple the origin of the moraine, recent investigations have concentrated on delineating the discernible glacigenic deposits (or landform architectural elements) which comprise the complex mosaic of the Oak Ridges Moraine. This study investigates the sedimentology of the Bloomington fan complex, one of the oldest elements of the Oak Ridges Moraine. The main sediment body of the Bloomington fan complex was deposited during early stages of the formation of the Oak Ridges Moraine, when the ice subdivided, and formed a confined, interlobate lake basin between the northern and southern lobes. Deposition from several conduits produced a fan complex characterized by multiple, laterally overlapping, fan bodies. It appears that the fans were active sequentially in an eastward direction, until the formation of the Bloomington fan complex was dominated by the largest fan fed by a conduit near the northeastern margin of the deposit. Following deposition of the fan complex, the northern and southern ice margins continued to retreat, opening drainage outlets to the west and causing water levels to drop in the lake basin. Glaciofluvial sediment was deposited at this time, cutting into the underlying fan complex. Re-advancing northern ice then closed westerly outlets, and caused water levels to increase, initiating the re-advance of the southern ice. As the southern ice approached the Bloomington fan, it deposited an ice-marginal sediment complex consisting of glacigenic sediment gravity flows, and glaciolacustrine and glaciofluvial sediments exhibiting north and northwesterly paleocurrents. Continued advance of the southern ice, overriding the fan complex, ii produced large-scale glaciotectonic deformation structures, and deposited the Halton Till. The subaqueous fan depositional model that is postulated for the Bloomington fan complex differs from published models due to the complex facies associations produced by the multiple conduit sources of sediment feeding the fans. The fluctuating northern and southern ice margins, which moved across the study area in opposite directions, controlled the water level in the interlobate basin and caused major changes in depositional environments. The influence of these two lobes also caused deposition from two distinct source directions. Finally, erosion, deposition, and deformation of the deposit with the readvance of the southern ice contributed further to the complexity of the Bloomington fan complex.

Role of trace element of the growth and Physiology of selected microalgae

The main objective of the study is primarily to determine the magnitude of selected trace elements, the concentrations of which would possibly accelerate growth resulting in larger biomass and sustained period of exponential phase for economically viable harvest. The study on the effect of three trace elements namely Cu, Mn and Zn on two species of algae,ISOChrySiS galbana Parke and Synechocystib salina Wislouch under different conditions of salinity, PH and temperature involves several combinations for each metal, from which the relative set of conditions has been adduced. The scheme of the experiments was statistically designed for interpretation of data and factors were assessed and graded according to relative importance. The methodology adopted for data interpretation is analysis of variance by split-plot design method. The thesis has been divided into five chapters. The introductory chapter explains the relevance of the research work undertaken. Chapter 11 gives a review on the work pertaining to the above mentioned three trace elements in relation to nutrition as well as on the toxic aspects about which there is an abundance of literature. Chapter Ill presents a detailed description of the material and specialised methods followed for the study. The results and conclusions of the various experiments on effect of metals on growth and other physiological activities are discussed in Chapters IV and V.

The R2 mobile element of Rhynchosciara americana: Molecular, cytological and dynamic aspects

Ribosomal RNA genes are encoded by large units clustered (18S, 5S, and 28S) in the nucleolar organizer region in several organisms. Sometimes additional insertions are present in the coding region for the 28S rDNA. These insertions are specific non-long terminal repeat retrotransposons that have very restricted integration targets within the genome. The retrotransposon present in the genome of Rhynchosciara americana, RaR2, was isolated by the screening of a genomic library. Sequence analysis showed the presence of conserved regions, such as a reverse transcriptase domain and a zinc finger motif in the amino terminal region. The insertion site was highly conserved in R. americana and a phylogenetic analysis showed that this element belongs to the R2 clade. The chromosomal localization confirmed that the RaR2 mobile element was inserted into a specific site in the rDNA gene. The expression level of RaR2 in salivary glands during larval development was determined by quantitative RT-PCR, and the increase of relative expression in the 3P of the fourth instar larval could be related to intense gene activity characteristic of this stage. 5`-Truncated elements were identified in different DNA samples. Additionally, in three other Rhynchosciara species, the R2 element was present as a full-length element.

The element of sacrifice in romantic love

In romantic love, the link with transgression is made very frequently indeed, so frequently that we cannot simply be dealing with a way of construing as real an obstacle that is imagined because the actual satisfaction is prohibited. Rather, the fact that access to the object is seen as transgressive must be due to the fact that the object itself has something that prevents the relationship from occurring. That is, it is not the case that the object is merely imagined to be inaccessible when in actual fact there is no obstacle to satisfaction by means of it. Rather, the relationship to the object as source of love is also and at the same time a perverse relationship that, at least for a neurotic, causes anxiety.

On monotonic sufficient conditions of fuzzy inference systems and their applications

An important and difficult issue in designing a Fuzzy Inference System (FIS) is the specification of fuzzy sets and fuzzy rules. In this paper, two useful qualitative properties of the FIS model, i.e., the monotonicity and sub-additivity properties, are studied. The monotonic sufficient conditions of the FIS model with Gaussian membership functions are further analyzed. The aim is to incorporate the sufficient conditions into the FIS modeling process, which serves as a simple (which can be easily understood by domain users), easy-to-use (which can be easily applied to or can be a part of the FIS model), and yet reliable (which has a sound mathematical foundation) method to preserve the monotonicity property of the FIS model. Another aim of this paper is to demonstrate how these additional qualitative information can be exploited and extended to be part of the FIS designing procedure (i.e., for fuzzy sets and fuzzy rules design) via the sufficient conditions (which act as a set of useful governing equations for designing the FIS model). The proposed approach is able to avoid the "trial and error" procedure in obtaining a monotonic FIS model. To assess the applicability of the proposed approach, two practical problems are examined. The first is an FIS-based model for water level control, while the second is an FIS-based Risk Priority Number (RPN) model in Failure Mode and Effect Analysis (FMEA). To further illustrate the importance of the sufficient conditions as the governing equations, an analysis on the consequences of violating the sufficient conditions of the FIS-based RPN model is presented.

Element of romanticization : sensory and spatial locations in the narratives of Indian diaspora in Australia

The “element of romanticization” or the constant yearning for ones roots—sensory and spatial locations—has become a phenomenon for different immigrant groups. As it is the politics of sensory and spatial locations that act as one of the core features that join Indian Diaspora across continents. The short stories analysed in this paper attempt to provide an understanding of the variety of interpretations of the sensory and spatial locations. We can note that in most of these stories the immigrants try to bring the Indian subcontinent to Australia with them by using myths, legends, historical facts, etc. These immigrants besides using myths also display a proudest possession, which reminds them constantly of home. These objects or icons or elements from the past, which the immigrants carry with them as cultural products are used as helpers in making a sense out of the alien situation presented before them. The paper concludes with the assertion that Indian-Australian short stories act as an important expression of the Indian way of life in Australia and may also effectively help in removing misconceptions and better understandings of local conditions thus encouraging fellow Australians to see differently within the cultural context of India and Australia.

First description of B chromosomes in the Hyphessobrycon (Characiformes, Characidae) genus: a hypothesis for the extra element of Hyphessobrycon eques Steindachner, 1882

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The Bible in Medieval Love Lyrics: A Fundamental Element of European Poetry Books

The focus of this study is the relationship among three different manuscripts (Modena, Bibl. Estense, MS α.R.4.4; Firenze, Bibl. Laurenziana MS Rediano 9; and London, BL, MS Harley, 2253) and the poetry they transmit. The aim of this research is to show the ways that the Bible was used in the transmission of the lyric poetry in the three literatures that they represent: Occitan (primarily through Marcabru’s songs), Italian (through the love poetry of Guittone d’Arezzo), and Middle English (through the Harley love lyrics and the MS.’s primary scribe), in a medieval European context.