Analysis Of The Fluorescence Of Body Fluids On Different Surfaces And Times.

The use of screening techniques, such as an alternative light source (ALS), is important for finding biological evidence at a crime scene. The objective of this study was to evaluate whether biological fluid (blood, semen, saliva, and urine) deposited on different surfaces changes as a function of the age of the sample. Stains were illuminated with a Megamaxx™ ALS System and photographed with a Canon EOS Utility™ camera. Adobe Photoshop™ was utilized to prepare photographs for analysis, and then ImageJ™ was used to record the brightness values of pixels in the images. Data were submitted to analysis of variance using a generalized linear mixed model with two fixed effects (surface and fluid). Time was treated as a random effect (through repeated measures) with a first-order autoregressive covariance structure. Means of significant effects were compared by the Tukey test. The fluorescence of the analyzed biological material varied depending on the age of the sample. Fluorescence was lower when the samples were moist. Fluorescence remained constant when the sample was dry, up to the maximum period analyzed (60 days), independent of the substrate on which the fluid was deposited, showing the novelty of this study. Therefore, the forensic expert can detect biological fluids at the crime scene using an ALS even several days after a crime has occurred.

Immunoexpression of adrenergic receptors in detrusor from patients with prune belly syndrome: a digital quantification

Introduction: Prune belly syndrome (PBS) presents with large-capacity bladders, high compliance and post-void residual volumes. Operative and conservative treatments are controversial. When histologically compared to normal bladder, bladder outlet obstruction results in an up- or down-regulation of adrenoceptors. Our goal was to study the immunoexpression of adrenoceptors in detrusor from patients with PBS. Materials and methods: Bladder domes from PBS patients (n = 14) were studied (PBG). For normal controls, bladder specimens were obtained at adult surgery (n = 13) (CG1) and at child autopsy (n = 5) (CG2). Staining was performed using antibodies to alpha 1a, alpha 1b, alpha 1d and beta 3 adrenoceptors. Five to 10 images were captured on an optic microscope with a digital camera and analysed with Photoshop(R). The immunocyhistochemical index with arbitrary units was calculated and compared. Results: Mean age was 1.28, 64 and 1.41 years for PBG, CG1 and CG2, respectively. The immunohistochemical index with arbitrary units of alpha 1a receptors was 0.06 in PBG, 0.16 in CG1 and 0.14 in CG2 (p = 0.008); of alpha 1b 0.06, 0.06 and 0.07 (p = 0.781); and of alpha 1d 0.04, 0.04 and 0.05 (p = 0.618). Regarding beta 3 the respective values were 0.07, 0.14 and 0.10 (p = 0.378). Conclusion: Our results show a decrease in ala-adrenoceptor immunostaining intensity in detrusor from children with PBS. Further in vitro studies are needed to determine whether these observations are physiologically significant. (C) 2009 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.

Monitorização do crescimento de um biofilme fotossintético por análise de imagem

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Conservação e Restauro

Morfologia e estabilidade de agregados superficiais de um argissolo vermelho-amarelo sob diferentes manejos de longa duração e mata atlântica secundária

Experimentos de longa duração submetidos a diferentes manejos de solo fornecem importantes informações quando as alterações nas propriedades físicas, químicas e biológicas são avaliadas e comparadas às das condições originais do solo. Este estudo teve como objetivo avaliar a morfologia externa e a estabilidade de agregados superficiais de um Argissolo Vermelho-Amarelo sob diferentes tipos de manejo em experimento de longa duração e nas condições sob Mata Atlântica secundária. Foram coletadas amostras de solo deformadas e indeformadas na camada de 0-5 cm, em dois tipos de manejo de solo: plantio direto (PD) e preparo com arado de disco + grade pesada (AD + GP). Também foi avaliada, como referência do estado de agregação original do solo, uma área de Mata Atlântica secundária (MS) adjacente ao experimento. As amostras indeformadas foram impregnadas com resina de poliéster e, na sequência, lâminas delgadas com 30 µm de espessura foram confeccionadas. Posteriormente, as lâminas foram examinadas em microscópio óptico, acoplado à câmera digital, para obtenção de imagens fotográficas mediante seleção aleatória de campos nas lâminas. As imagens geradas foram processadas pelo programa de computador Adobe Photoshop CS3 para análise de parâmetros relacionados à morfologia externa de agregados (perímetro, área, comprimento do maior eixo, comprimento do menor eixo, diâmetro de Feret, alongamento e arredondamento), que visam fornecer informações sobre o tamanho, a forma e a rugosidade dos agregados. Para as amostras deformadas, além da estabilidade dos agregados em água em classes de tamanho 4-2; 2-1; 1-0, 5; 0,5-0,25; 0,25-0,105; e < 0,105 mm de diâmetro, foi determinado o C orgânico total (COT). Foram calculados: diâmetro médio ponderado (DMP), diâmetro médio geométrico (DMG) e índice de estabilidade de agregados (IEA). Houve predomínio da classe de agregados de 4-2 mm nos dois tipos de manejo e na MS, sendo superior no solo sob MS e PD. Os índices de agregação DMG, DMP e IEA foram semelhantes no PD e na MS, sendo maiores que o do manejo por AD + GP. O PD promoveu diferenças morfológicas nos agregados em comparação ao AD + GP, mostrando proximidade com a referência MS.

Buccal dental microwear and stable isotopes of El Collado: A mesolithic site from Spain,

This study examines the correlation between buccal dental microwear and stable isotopes. The buccal surface of post-canine teeth casts from El Collado, the largest Mesolithic site in Spain, were examined under Scanning Electron Microscope; photomicrographs were taken from the middle third of the buccal surface with magnification 100X. Only six individuals passed the criteria for buccal dental microwear analysis. The photomicrographs were treated by adobe Photoshop 8.01 to cover an area 0.56 mm² of middle third of buccal surface, the output photomicrographs were digitized using Sigmascan Pro 5 by SPSS. Then the correlation between buccal microwear pattern and stable isotopes of the same individuals, of the previous study of Guixe et al., 2006, was examined using a Pearson test. Statistical analysis revealed that there is no significant correlation between stable isotopes and buccal dental microwear of the people of the Mesolithic site of El Collado. The historical and archaeological documentation suggest that the Mesolithic people tended to consume marine food. Fish-drying techniques were used during the Mesolithic period which allowed the introduction of dust and sand to the fish. These abrasive particles affected the buccal dental microwear pattern, so that no correlation between the isotopes and microwear may be expected. This also suggests that the buccal dental microwear pattern exceeds dietary reconstruction to reconstruct food processing techniques.

Buccal dental microwear and stable isotopes of El Collado: A mesolithic site from Spain,

This study examines the correlation between buccal dental microwear and stable isotopes. The buccal surface of post-canine teeth casts from El Collado, the largest Mesolithic site in Spain, were examined under Scanning Electron Microscope; photomicrographs were taken from the middle third of the buccal surface with magnification 100X. Only six individuals passed the criteria for buccal dental microwear analysis. The photomicrographs were treated by adobe Photoshop 8.01 to cover an area 0.56 mm² of middle third of buccal surface, the output photomicrographs were digitized using Sigmascan Pro 5 by SPSS. Then the correlation between buccal microwear pattern and stable isotopes of the same individuals, of the previous study of Guixe et al., 2006, was examined using a Pearson test. Statistical analysis revealed that there is no significant correlation between stable isotopes and buccal dental microwear of the people of the Mesolithic site of El Collado. The historical and archaeological documentation suggest that the Mesolithic people tended to consume marine food. Fish-drying techniques were used during the Mesolithic period which allowed the introduction of dust and sand to the fish. These abrasive particles affected the buccal dental microwear pattern, so that no correlation between the isotopes and microwear may be expected. This also suggests that the buccal dental microwear pattern exceeds dietary reconstruction to reconstruct food processing techniques.

Validação de escala diagramática para estimativa de desfolha provocada pela ferrugemasiática em soja

Este estudo teve como objetivo avaliar a precisão e acurácia de avaliadores com o uso de escala diagramática, em estimativas de desfolha provocada por doenças em soja. A escala apresenta seis níveis de severidade de desfolha (5, 15, 45, 65, 85 e 100%). As imagens utilizadas para avaliação foram obtidas em parcelas atacadas por ferrugem asiática (Phakopsora pachyrhizi), apresentando diferentes níveis de desfolha. A validação foi feita por dez avaliadores, utilizando-se como parâmetro de comparação dados reais da área com cobertura foliar, obtidos através de processamento de imagens, nos softwares Paint Shop Pro 4, e Adobe Photoshop 7.0. Quando não foi utilizada a escala, os avaliadores apresentaram tendência de subestimar a severidade de desfolha nos níveis mais baixos e de superestimar em níveis altos. A acurácia e precisão dos avaliadores aumentaram com o auxílio da escala diagramática.

Associação entre os polimorfismos HaeIII e MspI do gene para o receptor alfa de estrogênio e densidade mamográfica em mulheres após a menopausa

OBJETIVO: Avaliar a presença dos polimorfismos HaeIII e MspI do gene para o receptor de estrogênio alfa, bem como fatores clínicos e suas possíveis associações com a densidade mamográfica em mulheres após a menopausa. MÉTODOS: Foram avaliadas 115 mulheres após a menopausa, não usuárias de terapia hormonal e sem lesão mamária clínica ou mamograficamente identificada. A densidade mamográfica foi determinada por três observadores independentes, tomando-se como base a classificação dos padrões mamográficos do ACR-BIRADS®, 2003 (duas avaliações subjetivas e uma computadorizada - Adobe Photoshop® 7.0). Amostras de raspado bucal foram obtidas para extração de DNA e em seguida foi realizada uma PCR-RFLP (Polymerase Chain Reation - Restriction Fragment Length Polymorphism) para análise de polimorfismos presentes no íntron 1 e éxon 1 do gene do REalfa (HaeIII e MspI). RESULTADOS: O polimorfismo HaeIII foi encontrado em 43 (37,4%) das 115 mulheres, ao passo que o MspI estava presente em 96 (83,5%) das mesmas. Houve alto grau de concordância entre os três observadores na determinação da densidade mamográfica. Trinta e quatro (29,6%) mulheres tinham mamas densas, e 81 (70,4%), mamas lipossubstituídas. CONCLUSÃO: Não houve associação entre o polimorfismo HaeIII do gene para o receptor de estrogênio alfa e densidade mamográfica (Fisher = 0,712). Houve associação próxima à significância estatística entre o polimorfismo MspI e densidade (Fisher = 0,098). Idade, paridade e índice de massa corporal mostraram-se associados com densidade mamográfica.

Measurement of growth in the lichen Rhizocarpon geographicum using a new photographic technique

Lichenologists and users of lichenometry have long used calipers or photogrammetry to measure the growth of crustose lichens. Now, digital photography and popular computer software provide methodological alternatives. This thesis developed and tested a new methodology for tracking change and growth of the lichen, Rhizocarpon geographicum. Adobe Photoshop CS3 Extended software and a photographic time series (1996,2003,2006 and 2007) were used to measure thallus diameter, area, prothallus width and areolae area in 115 small R. geographicum thalli (0.53-1049.88 mm2 ). Measures of 8 diameters per thallus showed that change in diameter was highly variable and is a weak index of growth. Thallus area was a reliable measure of growth (power correlation, R2 = 0.89). Rapid, highly irregular growth occurred in small thalli «30 mm2 ), and steady, uniform growth occurred in larger thalli (>30 mm2 ). This new methodology is tedious but can potentially generate accurate and precise measures for even the tiniest of lichens.

Les enclaves résidentielles introverties dans la communauté métropolitaine de Montréal, une étape ultime vers la fermeture résidentielle.

Plusieurs logiciels utilisés dans la réalisation des pièces graphiques incluses dans ce mémoire : ArcGIS 10; Autocad 2012; GIS Consortium de la Ville de Chicago; PowerPoint 2010, Photoshop... etc. Notre recherche est la première et la seule qui aborde le phénomène de l'introversion résidentielle au Québec. Elle se focalise sur le postulat de la représentation du phénomène de l’introversion résidentielle autant qu’une forme urbaine fermée ou semi-fermée d’une descendance accouplée aux GCs étatsuniennes. De la gated community américaine vers l’enclave francilienne, à l’introversion résidentielle montréalaise. À l'aide de notre analyse typo-morphologique, basée principalement sur la théorie de Rossi (1966), nous avons dévoilé sur un nouveau fait urbain qui existe dans la communauté métropolitaine de Montréal. Nous l'avions désigné par : enclaves résidentielles introverties (ERI).

Multimedia Training Videos

Multimedia Training Videos is a series of free learning videos to show anyone interested in learning packages like Flash, Director and Photoshop.

eHandbook cube logo source files

The source files and full sized renders of the main eHandbook 'cube' (both Photoshop and .png format). Created by David Davies and Dr Matt Jones.

eHandbook title logo source files

Source files (Photoshop .psd) and full sized .png of the eHandbook title logo.

Revista digital Mouro.

El trabajo está publicado. Resumen basado en ficha elaborada por los autores. En el CD se encuentra material desarrollado por el grupo de trabajo

Realizacción de página web didáctica y creación de cd multimedia.

El trabajo no ha sido publicado, pero hay información del mismo, y se puede solicitar una copia del CD en www.lasalle.es/miguelbravo