DnaA-stimulated transcriptional activation of oriλ: Escherichia coli RNA polymerase β subunit as a transcriptional activator contact site

We present evidence that Escherichia coli RNA polymerase β subunit may be a transcriptional activator contact site. Stimulation of the activity of the pR promoter by DnaA protein is necessary for replication of plasmids derived from bacteriophage λ. We found that DnaA activates the pR promoter in vitro. Particular mutations in the rpoB gene were able to suppress negative effects that certain dnaA mutations had on the replication of λ plasmids; this suppression was allele-specific. When a potential DnaA-binding sequence located several base pairs downstream of the pR promoter was scrambled by in vitro mutagenesis, the pR promoter was no longer activated by DnaA both in vivo and in vitro. Therefore, we conclude that DnaA may contact the β subunit of RNA polymerase during activation of the pR promoter. A new classification of prokaryotic transcriptional activators is proposed.

Orbital and Physical Properties of the σ Ori Aa, Ab, B Triple System

We provide a complete characterization of the astrophysical properties of the σ Ori Aa, Ab, B hierarchical triple system and an improved set of orbital parameters for the highly eccentric σ Ori Aa, Ab spectroscopic binary. We compiled a spectroscopic data set comprising 90 high-resolution spectra covering a total time span of 1963 days. We applied the Lehman-Filhés method for a detailed orbital analysis of the radial velocity curves and performed a combined quantitative spectroscopic analysis of the σ Ori Aa, Ab, B system by means of the stellar atmosphere code FASTWIND. We used our own plus other available information on photometry and distance to the system for measuring the radii, luminosities, and spectroscopic masses of the three components. We also inferred evolutionary masses and stellar ages using the Bayesian code BONNSAI. The orbital analysis of the new radial velocity curves led to a very accurate orbital solution of the σ Ori Aa, Ab pair. We provided indirect arguments indicating that σ Ori B is a fast-rotating early B dwarf. The FASTWIND+BONNSAI analysis showed that the Aa, Ab pair contains the hottest and most massive components of the triple system while σ Ori B is a bit cooler and less massive. The derived stellar ages of the inner pair are intriguingly younger than the one widely accepted for the σ Orionis cluster, at 3 ± 1 Ma. The outcome of this study will be of key importance for a precise determination of the distance to the σ Orionis cluster, the interpretation of the strong X-ray emission detected for σ Ori Aa, Ab, B, and the investigation of the formation and evolution of multiple massive stellar systems and substellar objects.

Noi, esplorat-t-ori. Quanti sentieri ci servono per leggerci?

Cosa ci spinge a viaggiare? Siamo così terribilmente annoiati dalle nostre insicurezze e dalle luccicanti certezze della società che per rimanere in vita sentiamo il bisogno di muoverci, spostarci, andare fuori. Ma fuori dove? Crediamo davvero di salvarci andando a cercare qualcosa che non riusciamo nemmeno a definire? Coloro che potrebbero rispondere affermativamente a un tale quesito non sono quelli che partono, ma quelli che non tornano. Viaggiare per me significa leggere le differenze e leggermi nel profondo, scrivere dell’esperienza e scrivermi tracciando il mio cambiamento, tradurre da una lingua all’altra e tradurre le mie nuove emozioni. Questo elaborato è, perciò, l’umile tentativo di descrivere la complessità e l’arricchimento che può derivare dal fare esperienza, che sia viaggiando, leggendo, scrivendo o traducendo, vedendomi “di spalle che partivo” come avrebbe detto Fabrizio De André. Il fil rouge che creerà un legame tra tali azioni, che tesserà la trama dell’arazzo della mia esperienza è la metafora. La metafora è qualcosa che va anche al di là della sua semplice etimologia dal greco che la rende protagonista di un portare oltre. Metterò in scena il dubbio e la curiosità come motivi del viaggiare; parlerò della soglia come sipario che scorre via una volta che ci si apre all’Altro e non vi è più distinzione né distanza di spazio e tempo; varcherò i confini delle definizioni come palcoscenico in cui lettori, scrittori e traduttori viaggiano, e mostrerò come questi siano antropologi, registi e spettatori allo stesso tempo. Tutto ciò che infine rimarrà non sarà solo il silenzio, l’assenza derivanti dalle perdite che tali esperienze comportano, ma soprattutto l’amore che l’accoglienza dell’Altro insegna.

Nupcial : novela oríginal /

Mode of access: Internet.

Ashmat Shomron : sipur hisṭori mi-yeme Aḥaz melekh Yehudah u-Feḳaḥ ben Remalyahu ṿe-Hosheʻa ben Elah malkhe Yiśraʾel.

Mode of access: Internet.

Orígen y significación de algunas frases, locuciones, refranes,

Mode of access: Internet.

Bolívar y la emancipación de las colonias españolas desde los orígenes hasta 1815; obra premiada (el texto francés) por la Académie française con el premio Marcellin Guérin. Con un retrato y un mapa,

1. Orígenes de la revolución sudamericana.--2. El precursor, Miranda.--3. Bolívar.

Yiśraʼel Beshṭ : ḥizayon hisṭori be-ḥamesh maʻarakhot.

Cover title: Baal-Shem.

Mi-gibore ha-umah : tsiyur hisṭori = Migibore gauma /

Mode of access: Internet.

Shichiri ferry, Atsuta Temple, Yama-ai village (Miya Shichiri-no-watashi Atsutadera-ori Yama-ai no sato), #42, 53 Stations of the Tokaido (Tokaido Gojusan-tsugi no uchi)

Ando Hiroshige; 9 19/64 in. x 1 ft. 2 9/64 in.; woodcut (Oban)

Indigenous Māori values and perspectives to inform freshwater management in Aotearoa-New Zealand

In response to widespread water quality and quantity issues, the New Zealand Government has recently embarked on a number of comprehensive freshwater management reforms, developing a raft of national discussion and policy documents such as “Freshwater Reform 2013 and Beyond” and a National Policy Statement for freshwater management (NPS-FM 2014). Recent resource management reforms and amendments (RMA 2014), based on previous overarching resource management legislation (RMA 1991), set out a new approach and pathway to manage freshwater nationwide. Internationally, there is an increasing trend to engage with indigenous communities for research and collaboration, including indigenous groups as active participants in resource management decision making. What is driving this change toward more engagement and collaboration with indigenous communities is different for each country, and we document the progress and innovation made in this area in New Zealand. The indigenous rights of Māori in New Zealand are stated in the 1840 Treaty of Waitangi and in many forms of New Zealand's legislation. Local and central governments are eager to include local indigenous Māori groups (iwi/hapū) in freshwater management planning processes through meaningful engagement and collaboration. Key to the success of collaborative planning processes for Māori are enduring relationships between local government and Māori, along with adequate resourcing for all partners contributing to the collaborative process. A large number of shared governance and management models for natural resource management have emerged in New Zealand over the past 20 years, and some recent examples are reviewed. We provide some discussion to improve understanding and use of the terms used in these management models such as cogovernance, comanagement, and coplanning, and describe some of the more important frameworks and tools being developed with Māori groups (e.g., iwi/hapū), to strengthen Māori capacity in freshwater management and to support good collaborative process and planning.

The Holy Bible in the Oríyá language : a new translation, with the former versions in Bangálí and Oríyá diligently compared and revised.

Added title in Oriya.

Innovative cartography standards for Web-GIS portals : case study of the 'Survey of Israel's' Web-GIS Portal

Maps have been published on the world wide web since its inception (Cartwright, 1999) and are still accessed and viewed by millions of users today (Peterson, 2003). While early webbased GIS products lacked a complete set of cartographic capabilities, the functionality within such systems has significantly increased over recent years. Functionalities once found only in desktop GIS products are now available in web-based GIS applications, for example, data entry, basic editing, and analysis. Applications based on web-GIS are becoming more widespread and the web-based GIS environment is replacing the traditional desktop GIS platforms in many organizations. Therefore, development of a new cartographic method for web-based GIS is vital. The broad aim of this project is to examine and discuss the challenges and opportunities of innovative cartography methods for web-based GIS platforms. The work introduces a recently developed cartographic methodology, which is based on a web-based GIS portal by the Survey of Israel (SOI). The work discusses the prospects and constraints of such methods in improving web-GIS interfaces and usability for the end user. The work also tables the preliminary findings of the initial implementation of the web-based GIS cartographic method within the portal of the Survey of Israel, as well as the applicability of those methods elsewhere.

Web-based public participatory GIS

Decision support systems (DSS) have evolved rapidly during the last decade from stand alone or limited networked solutions to online participatory solutions. One of the major enablers of this change is the fastest growing areas of geographical information system (GIS) technology development that relates to the use of the Internet as a means to access, display, and analyze geospatial data remotely. World-wide many federal, state, and particularly local governments are designing to facilitate data sharing using interactive Internet map servers. This new generation DSS or planning support systems (PSS), interactive Internet map server, is the solution for delivering dynamic maps and GIS data and services via the world-wide Web, and providing public participatory GIS (PPGIS) opportunities to a wider community (Carver, 2001; Jankowski & Nyerges, 2001). It provides a highly scalable framework for GIS Web publishing, Web-based public participatory GIS (WPPGIS), which meets the needs of corporate intranets and demands of worldwide Internet access (Craig, 2002). The establishment of WPPGIS provides spatial data access through a support centre or a GIS portal to facilitate efficient access to and sharing of related geospatial data (Yigitcanlar, Baum, & Stimson, 2003). As more and more public and private entities adopt WPPGIS technology, the importance and complexity of facilitating geospatial data sharing is growing rapidly (Carver, 2003). Therefore, this article focuses on the online public participation dimension of the GIS technology. The article provides an overview of recent literature on GIS and WPPGIS, and includes a discussion on the potential use of these technologies in providing a democratic platform for the public in decision-making.

Rethinking health planning : a framework for organising information to underpin collaborative health planning

The field of collaborative health planning faces significant challenges created by the narrow focus of the available information, the absence of a framework to organise that information and the lack of systems to make information accessible and guide decision-making. These challenges have been magnified by the rise of the ‘healthy communities movement’, as a result of which, there have been more frequent calls for localised, collaborative and evidence-driven health related decision-making. This paper discusses the role of decision support systems as a mechanism to facilitate collaborative health decision-making. The paper presents a potential information management framework to underpin a health decision support system and describes the participatory process that is currently being used to create an online tool for health planners using geographic information systems. The need for a comprehensive information management framework to guide the process of planning for healthy communities has been emphasised. The paper also underlines the critical importance of the proposed framework not only in forcing planners to engage with the entire range of health determinants, but also in providing sufficient flexibility to allow exploration of the local setting-based determinants of health.