Comparison of a multiplex-PCR assay with mycolic acids analysis and conventional methods for the identification of Mycobacteria

A fast, sensitive and cost-effective multiplex-PCR assay for Mycobacterium tuberculosis complex (MTC) and Mycobacterium avium (M. avium) identification for routine diagnosis was evaluated. A total of 158 isolates of mycobacteria from 448 clinical specimens from patients with symptoms of mycobacterial disease were analyzed. By conventional biochemical methods 151 isolates were identified as M. tuberculosis, five as M. avium and two as Mycobacterium chelonae (M. chelonae). Mycolic acid patterns confirmed these results. Multiplex-PCR detected only IS6110 in isolates identified as MTC, and IS1245 was found only in the M. avium isolates. The method applied to isolates from two patients, identified by conventional methods and mycolic acid analysis, one as M. avium and other as M. chelonae, resulted positive for IS6110, suggesting co-infection with M. tuberculosis. These patients were successfully submitted to tuberculosis treatment. The multiplex-PCR method may offer expeditious identification of MTC and M. avium, which may minimize risks for active transmission of these organisms and provide useful treatment information.

Influence of Fc gamma RIIIb polymorphism on its ability to cooperate with Fc gamma RIIa and CR3 in mediating the oxidative burst of human neutrophils

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Do we really need 24-h observation for patients with minimal brain injury and small intracranial bleeding? The Bernese Trauma Unit Protocol

Traumatic brain injury is one of the most common reasons for admission to hospital emergency departments. However, optimal diagnosis and treatment protocols remain controversial. The aim of this study is to assess whether a specific group of patients can be discharged from the hospital without 24-h neurological observation.

Multiple colonization with S. pneumoniae before and after introduction of the seven-valent conjugated pneumococcal polysaccharide vaccine

Simultaneous carriage of more than one strain of Streptococcus pneumoniae promotes horizontal gene transfer events and may lead to capsule switch and acquisition of antibiotic resistance. We studied the epidemiology of cocolonization with S. pneumoniae before and after introduction of the seven-valent conjugated pneumococcal vaccine (PCV7).

Taylor's sacred minstrel, or, American church music book : a new collection of psalm and hymn tunes adapted to the various metres now in use : together with anthems, sentences, chants and other pieces : for the use of choirs, congregations, singing schools, musical societies, social sacred music circles, and private devotion ... /

Mode of access: Internet.

The Virgin Mary with Saints Thomas Aquinas and Paul: detail of the Virgin Mary

Bernardo Daddi; tempera and gold leaf on panel

United States census of agriculture: 1925 : Reports for states, with statistics for counties and a summary for the United States /

At head of title: Department of Commerce. Herbert Hoover, secretary. Bureau of the Census. W.M. Steuart, director.

Deering's Political code of the state of California : adopted March 12, 1872, with amendments up to and including those of the fifty-second session of the Legislature, 1937. /

Includes index.

Handbook to the grammar of the Greek Testament. Together with a complete vocabulary, and an examination of the chief New Testament synonyms.

Preface signed: Samuel G. Green.

An introduction to the Italian languaje : containing specimens both of prose and verse, selectes from Francesco Redi, Galileo Galilei, [etc.] ... with a literal translation and grammatical notes, for the use of those who being already acquainted with grammar, attempt to learn it without a master /

Mode of access: Internet.

The poetical works of Charles Churchill : with memoir, critical dissertation, and explanatory notes / by the Rev. George Gilfillan.

Mode of access: Internet.

Aischylou Choēphoroi. The Choephoroe of Aeschylus. A new edition of the text with notes, critical, explanatory, and philological, designed for the use of students in the universities.

Mode of access: Internet.

An essay on Junius and his letters : embracing a sketch of the life and character of William Pitt, Earl of Chatham, and memoirs of certain other distinguished individuals : with reflections historical, personal, and political, relating to the affairs of Great Britain and America from 1763 to 1785 /

Includes bibliographical references.