Vapaa-ajan vieton luonnonvarojen kulutus

This thesis studies the use of natural resources for leisure time activities. The method used is Material Input per Service Unit (MIPS method). Leisure time has an increasing effect on the material flows of households, and that way has a bigger pressure on the environment. The most popular way of spending spare time in Finland is to watch TV and to listen to music or radio. Regardless of these, this thesis takes a closer look at boating, playing a musical instrument and visiting a theatre and tries to quantify their material flows. MIPS calculations of this thesis are case-studies and do not tell the whole truth about the hobbies. The aim was to have an overview about the magnitude of the activities. In the boating calculations, inside the system boundaries there are the boat itself, transport of the boat, outboard motor, gasoline consumption of the outboard motor, travelling to and from the harbour, and the harbour infrastructure. Calculations of playing a music instrument consider the instrument itself, music school and its maintenance, and travelling to the school. In the case of theatre the included things are theatre house and its maintenance, decor and costumes of the plays, transport of the decor, and travelling of the audience. The results of this thesis suggest that the biggest material flow of boating comes from travelling to and from the harbour and from the harbour infrastructure. The gasoline consumption of the outboard motor also makes a difference. One hour of boating with a rowing boat consumes 1 kg of abiotic materials. Boating with an outboard motorboat consumes astonishing 113 kg of abiotic resources. Visiting a music lesson for one hour consumes 9 kg of abiotic resources when travelling there by bus. One hour in a theatre play consumes 17 kg of abiotic materials when travelling by bus. Transport has a significant role on the resource consumption of leisure time activities.

Kaffe在龙芯2E上的移植

为了在龙芯2E处理器上建立稳定的Java运行环境,丰富龙芯平台的上层软件库,以Kaffe这款开源Java虚拟机为移植对象,分析了其运行机制,确定了其代码结构中平台相关的3个主要模块:SysCallMethod、Trampoline和JTT,并结合龙芯2E处理器的特点,给出了相关模块的修改方案.最后用第三方的测试标准对移植后的虚拟机进行了测试,表明了移植的有效性.

基于编译器的瞬时故障容错技术研究与实现

随着计算机芯片的速度不断提升，器件的门限电压越来越低，因此单粒子翻转的瞬时故障越来越容易发生。特别是在太空环境中的计算机系统，在宇宙射线的影响下，瞬时故障更为频繁，系统可靠性面临更突出的考验。 为了提高计算机系统的可靠性，一般有硬件冗余容错和软件冗余容错两种方法。相对硬件容错而言，软件容错的优点是价格便宜，性价比高，配置灵活等，缺点是会带来额外的时间和空间开销，而且给程序员带来编写额外的容错代码的工作量。近来出现了一些基于编译的软件容错方法，可在编译的过程中自动加入冗余容错逻辑，但是这类编译容错方法仍然会带来显著的时间空间开销。如何在保持容错能力的同时尽量降低时空开销，是有待继续研究的问题。 本文在编译容错方向上进行了进一步研究和实现，提出利用源代码中的变量信息对冗余容错逻辑进行了剪裁，在保证容错能力的同时降低了时空开销，对内存和寄存器中的数据进行保护。具体内容有： 1. 提出了一个容错编译环境SCC的设计蓝图，构建了一个容错编译工具的远 景目标。 2. 提出了一种指令级的编译容错检测方法VarBIFT ，提供检测瞬时故障的能力。平均只利用0.0069倍的时间损耗和0.3620倍的空间损耗就将发生瞬时故障时，程序正确执行和检测到故障的概率总和平均从39.1%提升到76.9%， 3. 提出了一种指令级的编译容错恢复方法VarRIFT ，提供从瞬时故障中恢复正确数据的能力。平均只增加0.043倍的时间损耗和0.69倍的空间损耗就将发生瞬时故障时，程序仍然正确执行的概率平均从44.8%提升到了78.7%。 4. 基于开源编译器LCC，实现了上述两个编译容错方法VarBIFT 和VarRIFT 。在容错方法的实现中只修改了跟具体CPU指令相独立的中间逻辑，所以这两个实现能够方便得移植到SPARC、MIPS等其他CPU架构上。 5. 开发了一个故障注入工具，并用它测试了上述两个编译容错方法VarBIFT和VarRIFT 的容错能力。

Kaffe虚拟机在龙芯2E上的移植研究

Java虚拟机作为Java运行环境的核心，已成为现代计算机平台不可缺少的组成部分。Java虚拟机和平台间的兼容性直接决定了上层Java程序的运行的稳定性。龙芯2E是由中科院计算机研究所自主研发的高性能微处理器。由于采用MIPS指令集，现有Java虚拟机对龙芯的支持并不理想，这极大地限制了龙芯平台的上层应用。 针对这一问题，本文对现有虚拟机进行了分析，选择了移植性较强的Kaffe虚拟机作为移植对象，力求在龙芯2E上建立一个稳定的Java运行环境。以此为目的，本文整理了龙芯2E相关的内容，包括龙芯2E的指令集特点，龙芯下参数传递的方式，龙芯下函数栈的组织等等；研究了Java虚拟机规范中，一般Java虚拟机的体系结构；分析了Kaffe虚拟机的代码结构，确定了移植需要修改的三个主要模块：本地代码调用接口，Trampoline技术和JIT3引擎；给出了移植方案。其中，JIT3引擎作为整个虚拟机的核心，是整个移植工作的重点。本文对其编译过程，寄存器分配策略，Java栈的组织方式，以及标签，常量池等平台相关机制进行了深入的分析。在此基础上，修改了Java栈向本地栈的映射方式，实现了标签机制中平台相关部分的代码，重新描述了寄存器信息并重新实现了本地汇编指令。 针对Java虚拟机调试困难的问题，本文总结了龙芯平台下Kaffe虚拟机的调试方法。在调试过程中发现了Kaffe原有代码中的编码错误，并给出了修改方案。 最后采用第三方的评测工具对移植后的虚拟机进行测试，验证了移植的有效性。

Molecular imprinting of nitrophenol and hydroxybenzoic acid isomers: effect of molecular structure and acidity on imprinting

Three nitrophenol isomer-imprinted polymers were prepared under the same conditions using 4-vinylpyridine as a functional monomer. Different recognition capacities for template molecules were observed for the three polymers. Another imprinting system with stronger acidity than nitrophenol isomers, 2-hydroxybenzoic acid (salicylic acid) and 4-hydroxybenzoic acid, was imprinted using 4-vinylpyridine or acrylamide as functional monomer respectively. Both 4-hydroxybenzoic acid-imprinted polymers using the two monomers showed recognition ability for the template molecule. However, when acrylamide was chosen as functional monomer, the salicylic acid-imprinted polymer showed very weak recognition for the template molecule, whereas strong recognition ability of the resultant polymer for salicylic acid was observed with 4-vinylpyridine as functional monomer. It seems that the structure and acidity of template molecules is responsible for the difference in recognition, by influencing the formation and strength of interaction between template molecule and functional monomer during the imprinting process. An understanding of the mechanism of molecular imprinting and molecular recognition of MIPs will help to predict the selectivity of MIPs on the basis of template molecule properties. Copyright (C) 2003 John Wiley Sons, Ltd.

Sensitive biomimetic sensor based on molecular imprinting at functionalized indium tin oxide electrodes

We initially report an electrochemical sensing platform based on molecularly imprinted polymers (MIPs) at functionalized Indium Tin Oxide Electrodes (ITO). In this research, aminopropyl-derivatized organosilane aminopropyltriethoxysilane (APTES), which plays the role of functional monomers for template recognition, was firstly self-assembled on an ITO electrode and then dopamine-imprinted sol was spin-coated on the modified surface. APTES which can interact with template dopamine (DA) through hydrogen bonds brought more binding sites located closely to the surface of the ITO electrode, thus made the prepared sensor more sensitive for DA detection. Potential scanning is presented to extract DA from the modified film, thus DA can rapidly and completely leach out. The affinity and selectivity of the resulting biomimetic sensor were characterized using cyclic voltammetry (CV). It exhibited an increased affinity for DA over that of structurally related molecules, the anodic current for DA oxidation depended on the concentration of DA in the linear range from 2 x 10(-6) M to 0.8 x 10(-3) M with a correlation coefficient of 0.9927.In contrast, DA-templated film prepared under identical conditions on a bare ITO showed obviously lower response toward dopamine in solution.

Predicting gene function in Saccharomyces cerevisiae

Clare, A. and King R.D. (2003) Predicting gene function in Saccharomyces cerevisiae. 2nd European Conference on Computational Biology (ECCB '03). (published as a journal supplement in Bioinformatics 19: ii42-ii49)

Hierarchical Multi-classification with Predictive Clustering Trees in Functional Genomics

Struyf, J., Dzeroski, S. Blockeel, H. and Clare, A. (2005) Hierarchical Multi-classification with Predictive Clustering Trees in Functional Genomics. In proceedings of the EPIA 2005 CMB Workshop

How well do we understand the clusters found in microarray data?

Clare, A. and King R.D. (2002) How well do we understand the clusters found in microarray data? In In Silico Biol. 2, 0046

Machine learning of functional class from phenotype data

Clare, A. and King R.D. (2002) Machine learning of functional class from phenotype data. Bioinformatics 18(1) 160-166

An enantio-selective chromatographic stationary phase for S-ibuprofen prepared by stoichiometric molecular imprinting

Molecularly Imprinted Polymers (MIPs) against S-ibuprofen were synthesised using a tailor made functional monomer, 2-acrylamido-4-methylpyridine, following extensive pre-polymerisation studies of template-monomer complexation. An apparent association constant of 340 +/- 22 M-1 was calculated that was subsequently corrected to account for dimerisation of ibuprofen (K-dim = 320 +/- 95 M-1) resulting in an intrinsic association constant of 715 +/- 16 M-1, consistent with previously reported values. Using the synthesised imprinted polymer as a stationary phase, complete resolution of a racemic mixture of ibuprofen was achieved in predominantly aqueous mobile phases. An imprinting factor of 10 was observed, and was found to be in agreement with the difference in the average number of binding sites between MIP and blank polymers, calculated by staircase frontal chromatography. The imprinted polymers exhibited enhanced selectivity for the templated drug over structurally related NSAIDs. When applied as sorbents in solid-phase extraction of ibuprofen from commercial tablets, urine and blood serum samples, recoveries up to 92.2% were achieved. © The Royal Society of Chemistry 2012

Synthesis of caffeic acid and p-hydroxybenzoic acid molecularly imprinted polymers and their application for the selective extraction of polyphenols from olive mill waste waters

Using caffeic acid and p-hydroxybenzoic acid as templates, two molecularly imprinted polymers (MIPs) were prepared that were used for isolation of polyphenols from olive mill waste water samples (OMWWs) without previous pre-treatment. For the preparation of the caffeic acid MIPs 4-vinylpyridine, allylurea, allylaniline and methacrylic acid were tested as functional monomers, ethylene glycol dimethylacrylate (EDMA), pentaerythritol trimethylacrylate (PETRA) and divinylbenzene 80 (DVB80) as cross-linkers and tetrahydrofuran as porogen. For p-hydroxybenzoic acid 4-vinylpyridine, allylurea and allylaniline were tested as functional monomers, EDMA and PETRA as cross-linkers and acetonitrile as porogen. The performance of the synthesized polymers was evaluated against seven structurally related compounds by means of polymer-based HPLC. The two polymers that presented the most interesting properties were further evaluated by batch rebinding and from the derived isotherms their capacity and binding strength were determined. Using solid-phase extraction (SPE), their ability to recognize and bind the template molecule from an aqueous solution as well as the pH dependence of the binding strength were explored. After establishing the best SPE protocol, an aqueous model mixture of compounds and a raw OMWWs sample were loaded on the two best polymers. The result of the consecutive use of the two polymers on the same sample was explored. It was concluded that acidic conditions favour the recognition abilities of both polymers and that they can be used for a quick and efficient isolation of the polyphenol fraction directly from raw OMWW.

Preparation of a molecularly imprinted polymer for the solid-phase extraction of scopolamine with hyoscyamine as a dummy template molecule

Molecularly imprinted polymers (MIPs) selective for scopolamine were produced using hyoscyamine (a close structural analogue) as template molecule. The produced polymers were used as media for solid-phase extraction, exhibiting selective binding properties for the analyte from biological samples. Human and calf urine and serum were processed on the MIP under various extraction protocols. The best performance was observed after loading the analyte in aqueous environment facilitating retention on the MIP by non-selective hydrophobic interactions. The MIPs were subsequently washed using an optimised solvent system to enable selective desorption of the analyte. Other related and non-related compounds were accessed to evaluate molecular recognition properties. Recoveries of up to 79% were achieved for the analyte of interest from biological samples.

Non-covalent imprinting of phosphorous esters

The creation of molecularly imprinted polymers (MIPs) for the recognition of phosphate and phosphonate esters is reported. The single, weak hydrogen-bond acceptor site in these molecules has been targeted using a 1,3-diarylurea functional monomer. Polymers were prepared using either stoichiometric ratios of functional monomer to template or a large excess of the template during imprinting. The recognition properties of the polymers were assessed in the chromatographic mode for their ability to retain the templates and analogous analytes. The results are discussed with regards to the choice and amount of template, polymerisation conditions and the composition of the chromatographic mobile phase.

Molecularly imprinted polymers for the extraction of imiquimod from biological samples using a template analogue strategy

Molecularly Imprinted Polymers (MIPs) against imiquimod, a highly potent immune response modifier used in the treatment of skin cancer, were synthesised using a template analogue strategy and were compared with imprints of the drug itself. An investigation of the complexation between the functional monomer and the template analogue revealed an association constant of 1,376 ± 122 M-1, significantly higher than previously reported values for similar systems. The binding characteristics of the synthesised imprinted polymers were evaluated and extremely strong binding for imiquimod was observed while imprinting factors as high as 17 were calculated. When applied as sorbents in solid-phase extraction of imiquimod from aqueous, urine and blood serum samples, clean extracts and recoveries up to 95% were achieved, and it is concluded that while imiquimod imprints exhibited higher capacity for the drug, template analogue imprints are more selective. The results obtained suggest potential applications of imiquimod imprints as sorbents in rapid extraction and monitoring of undesirable systemic release of the drug.