Fleas : final report /

Pagination preceded by package no.: xi.

The skeletal anatomy of fleas (Siphonaptera) /

Bibliography: p. 70-73.

The fleas of North America : classification, identification, and geographic distribution of these injurious and disease-spreading insects /

Contribution from Bureau of Entomology and Plant Quarantine.

Fleas of public health importance and their control /

Mode of access: Internet.

Fleas of eastern United States,

"Selected bibliography": p. 121-124.

Duck fleas as evidence for eiderdown production on archaeological sites

Acknowledgements This project was undertaken as part of my doctoral studies funded by the Commonwealth Scholarship Commission (CACR-2009-39) in the United Kingdom. I would like to thank my supervisors Karen Milek and Andrew Dugmore for their help and support. I also wish to thank Jónas Helgason, his son Alexius Jónasson and Baldur Vilhelmsson for kindly having allowed access to the eiderdown stores and workshops at Æðey and Vatnsfjörður and for having provided assistance when needed. I would like to thank Fornleifastofnun Íslands for supporting my fieldwork at Vatnsfjörður, as well as Paul Ledger and Garðar Guðmundsson for their help during fieldwork. I am especially grateful to Richard Marriott for his invaluable help with flea identifications and for lending me reference material. Erling Ólafsson and Jan Klimaszewski also helped with the beetle identifications. Consultation of the BugsCEP database (Buckland and Buckland, 2006) aided the redaction of this paper.

Duck fleas as evidence for eiderdown production on archaeological sites

Acknowledgements This project was undertaken as part of my doctoral studies funded by the Commonwealth Scholarship Commission (CACR-2009-39) in the United Kingdom. I would like to thank my supervisors Karen Milek and Andrew Dugmore for their help and support. I also wish to thank Jónas Helgason, his son Alexius Jónasson and Baldur Vilhelmsson for kindly having allowed access to the eiderdown stores and workshops at Æðey and Vatnsfjörður and for having provided assistance when needed. I would like to thank Fornleifastofnun Íslands for supporting my fieldwork at Vatnsfjörður, as well as Paul Ledger and Garðar Guðmundsson for their help during fieldwork. I am especially grateful to Richard Marriott for his invaluable help with flea identifications and for lending me reference material. Erling Ólafsson and Jan Klimaszewski also helped with the beetle identifications. Consultation of the BugsCEP database (Buckland and Buckland, 2006) aided the redaction of this paper.

Duck fleas as evidence for eiderdown production on archaeological sites

Acknowledgements This project was undertaken as part of my doctoral studies funded by the Commonwealth Scholarship Commission (CACR-2009-39) in the United Kingdom. I would like to thank my supervisors Karen Milek and Andrew Dugmore for their help and support. I also wish to thank Jónas Helgason, his son Alexius Jónasson and Baldur Vilhelmsson for kindly having allowed access to the eiderdown stores and workshops at Æðey and Vatnsfjörður and for having provided assistance when needed. I would like to thank Fornleifastofnun Íslands for supporting my fieldwork at Vatnsfjörður, as well as Paul Ledger and Garðar Guðmundsson for their help during fieldwork. I am especially grateful to Richard Marriott for his invaluable help with flea identifications and for lending me reference material. Erling Ólafsson and Jan Klimaszewski also helped with the beetle identifications. Consultation of the BugsCEP database (Buckland and Buckland, 2006) aided the redaction of this paper.

First Report of the Isolation and Molecular Characterization of Rickettsia amblyommii and Rickettsia felis in Central America

During 2010, 15 adult ticks, identified as Amblyomma cajennense, were collected from horses in Cahuita and Turrialba districts, whereas 7 fleas, identified as Ctenocephalides felis, were collected from a dog in San Jose city, Costa Rica. In the laboratory, three A. cajennense specimens, two from Cahuita and one from Turrialba, were individually processed for rickettsial isolation in cell culture, as was a pool of seven fleas. Rickettsiae were successfully isolated and established in Vero cell culture from the three ticks and from a pool of seven fleas in C6/36 cell culture. The three tick isolates were genotypically identified as Rickettsia amblyommii, and the flea isolate was identified as Rickettsia felis through DNA sequencing of portions of the rickettsial genes gltA, ompA, and ompB of each isolate. In addition, other seven ticks were shown to contain rickettsial DNA. Polymerase chain reaction products of at least two of these ticks were sequenced and also showed to correspond to R. amblyommii. Overall, 66.7% (10/15) of the A. cajennense adult ticks were found to be infected with rickettsiae. This is the first report of a successful isolation in cell culture of R. amblyommii and R. felis from Central America.

Effects of azadirachtin on Ctenocephalides felis in the dog and the cat

Azadirachtin-containing neem seed extract is a powerful insect growth regulator, a feeding deterrent and repellent with low toxicity. Unfortunately, azadirachtin degrades rapidly in light, excessive heat or alkalinity. Evaluations of azadirachtin on ectoparasites on animals have been scarce. The purpose of this work was to describe the effects of normal and potentiated azadirachtin on Ctenocephalides felis in the dog or cat. Groups of kennelled greyhounds and domestic cats infested with C. felis were sprayed once with azadirachtin containing neem seed extract with or without diethyltoluamide (Deer) and/or citronella. Methanolic extracts with 200, 1000 or 2400 ppm azadirachtin reduced fleas in a dose-dependent manner. Compared with fleas counted on treated dogs just before treatment and untreated infested dogs, 1000-2400 ppm azadirachtin reduced fleas 93-53% for 19 days. However, combined with 500 ppm Deet and 33% w/v citronella, only 500 ppm azadirachtin reduced fleas 95-62% for 20 days. On cats inoculated with 50 fleas 2 days before treatment, the combination reduced fleas and eggs 100% to day 6 and 83-51% from days 7 to 9. On petri dishes, the combination achieved 100% egg mortality up to day 7 and 80% to day 14 and 38-52% to to days 21-28. Deet, with or without neem seed extract or citronella, and citronella, with or without neem, did not reduce fleas significantly. The results show that azadirachtin reduced fleas in a dose-dependent manner in flea-contaminated environments. In cats, the combination killed most fleas within 24 h, providing effective flea control for 7 days. The results suggest that Deet with citronella potentiated the effect of azadirachtin on C. felis. (C) 1998 Elsevier Science B.V.