Corning Museum of Glass, Corning, New York, 1976-1980

[Conceptual Sketch of of Museum of Glass I Site Plan], untitled. Ink sketch on blue-line print, 9 1/2 x 18 1/4 inches [from photographic copy by Lance Burgharrdt]

Washington Monument Visitor Center Competition, Washington, DC, 1972 (unbuilt project)

G.B. Worksheet, #43. Ink drawing on reverse of blue-line print, 14 1/4 x 18 3/4 inches [from photographic copy by Lance Burgharrdt]

Juma al-Majid Center for Culture and Heritage Competition, Dubai, United Arab Emirates, 1993 (unbuilt project)

[Schematic Design Drawing of East Elevation and] East-West Section. Black ink, colored ink and pencil drawing with marker coloring on two blue-line prints, 8 1/4 x 38 & 15 x 41 1/4 inches [from photographic copy by Lance Burgharrdt]

Juma al-Majid Center for Culture and Heritage Competition, Dubai, United Arab Emirates, 1993 (unbuilt project)

[Schematic Design Drawing of] East Elevation. Black and colored ink and pencil drawing with marker coloring on two blue-line prints, 9 1/4 x 38 & 9 1/2 x 41 1/2 inches [from photographic copy by Lance Burgharrdt]

University of Michigan Sports Services Building, Ann Arbor, Michigan, 1985-1990

[Schematic Design Sketch of] Section "C". Ink and red pencil drawing on blue-line print with blue and orange marker coloring, 12 1/4 x 40 3/4 inches

Ohio State University College of Law Building Addition, Columbus, Ohio 1988-1993

[Schematic Design Drawing of Site Plan], untitled. Digital image only of black and green ink drawing on blue-line print, initialed

Proceedings of the annual meeting of the New Hampshire Press Association

Proceedings for 1871-72, blue line edition

Map showing properties under jurisdiction of the Architect of the Capitol : [Capitol Hill, Washington, D.C.] /

Also shows government buildings and block numbers.

Map of the U.S. Soldiers' Home, Washington, D.C. /

Relief shown by contours and hachures.

Map showing areas comprising United States Capitol grounds /

Shows government buildings and boundaries of Capitol grounds.

Genotoxic, mutagenic and cytotoxic effects of the commercial dye CI Disperse Blue 291 in the human hepatic cell line HepG2

Textile dyes are discarded into the aquatic ecosystem via industrial effluents and potentially expose humans and local biota to adverse effects. The commercial dye CI Disperse Blue 291 which contains the aminoazobenzene 2-[(2-bromo-4,6-dinitrophenyl)azo]-5(diethylamino)-4-methoxyacetanilide (CAS registry no. 56548-64-2), was tested for genotoxicity and cytotoxicity in the human hepatoma cell line HepG2, using the comet assay, micronucleus (MN) test and a cell viability test. Five different concentrations of the test compound were examined: 200 mu g/ml, 400 mu g/ml, 600 mu g/ml, 800 mu g/ml and 1000 mu g/ml. An increase in comet tail length and in the frequency of MN was detected with exposure of cells to concentrations of the commercial dye from 400 pg/ml. Furthermore, the dye was found to decrease cell viability. The results of this study demonstrate for the first time the genotoxic and mutagenic effects of the dye CI Disperse Blue 291 in mammalian cells, thus stressing the need to develop non-mutagenic dyes and to invest in improving the treatment of effluents. These measures will help to prevent harmful effects that these compounds can have on humans and aquatic organisms that come in contact with them. (C) 2007 Elsevier Ltd. All rights reserved.

Discovering the effects of omega-3 and omega-6 fatty acids on allergy using a hek-blue cell line

In recent times, allergy has become a financial, physical andpsychological burden to the society as a whole. Allergic reactions can result in life-threatening situations causing morbidity and high economic cost. Therefore, more effective reagents are needed for allergy treatment. Literature suggests that a causal relationship exists between the intake of Omega-3/6 fatty acids such as DHA, EPA, DPA and AA and atopic individuals suffering from allergies. In an allergic cascade, cytokines IL-4 and IL-13 bind to IL-4 receptor (IL-4R), which activates the STAT6 phosphorylation pathway leading to gene activation of allergen-specific IgE production by B cells. The overall aim of this study is to characterise Omega-3/6 fatty acids and their effects on IgE production.

Discovering the effects of omega-6 fatty acids on allergy using a HEK-Blue cell line

In recent times, allergy has become a financial, physical and psychological burden to the society as a whole. Allergic reactions can result in life-threatening situations causing morbidity and high economic cost. Therefore, more effective reagents are needed for allergy treatment. Omega-6 fatty acids have gained attention in allergic studies mainly due to their inflammatory properties. Literature suggests that a causal relationship exists between the intake of omega-6 fatty acids such as DPA and AA and atopic individuals suffering from allergies. In an allergic cascade, cytokines IL-4 and IL-13 bind to IL-4 receptor (IL-4R), which activates the STAT6 phosphorylation pathway leading to gene activation of allergen-specific IgE production by B cells. Consequently, IgE production leads to clinical symptoms of allergy. The overall aim of this study is to characterise DPA and AA and their effects on IgE production.

Exploring the effects of omega-3 and omega-6 fatty Acids on allergy using a HEK-blue cell line.

BACKGROUND: Allergic reactions can result in life-threatening situations resulting in high economic costs and morbidity. Therefore, more effective reagents are needed for allergy treatment. A causal relationship has been suggested to exist between the intake of omega-3/6 fatty acids, such as docosahexanoic acid (DHA), eicosapentanoic acid (EPA), docosapentanoic acid (DPA) and arachidonic acid (AA), and atopic individuals suffering from allergies. In allergic cascades, the hallmark cytokine IL-4 bind to IL-4 receptor (IL-4R) and IL-13 binds to IL-13 receptor (IL-13R), this activates the STAT6 phosphorylation pathway leading to gene activation of allergen-specific IgE antibody production by B cells. The overall aim of this study was to characterize omega-3/6 fatty acids and their effects on STAT6 signaling pathway that results in IgE production in allergic individuals. METHODS: The fatty acids were tested in vitro with a HEK-Blue IL-4/IL-13 reporter cell line model, transfected with a reporter gene that produces an enzyme, secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a substitute to IgE when cells are stimulated with bioactive cytokines IL-4 and/or IL-13. RESULTS: We have successfully used DHA, EPA and DPA in our studies that demonstrated a decrease in SEAP secretion, as opposed to an increase in SEAP secretion with AA treatment. A statistical Student's t-test revealed the significance of the results, confirming our initial hypothesis. CONCLUSION: We have successfully identified and characterised DHA, EPA, DPA and AA in our allergy model. While AA was a potent stimulator, DHA, EPA and DPA were potential inhibitors of IL-4R/IL-13R signalling, which regulates the STAT6 induced pathway in allergic cascades. Such findings are significant in the future design of dietary therapeutics for the treatment of allergies.