Initiation of embryogenic cell suspensions of taro (Colocasia esculenta var. esculenta) and plant regeneration

Embryogenic callus was initiated by culturing in vitro taro corm slices on agar-solidified half-strength MS medium containing 2.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by transfer to 1.0 mg/L thidiazuron (TDZ). Callus was subsequently proliferated on solid medium containing 1.0 mg/L TDZ, 0.5 mg/L 2,4- D and 800 mg/L glutamine before transfer to liquid medium containing the same components but with reduced glutamine (100 mg/L). After 3 months in liquid culture on an orbital shaker, cytoplasmically dense cell aggregates began to form. Somatic embryogenesis was induced by plating suspension cells onto solid media containing reduced levels of hormones (0.1 mg/L TDZ, 0.05 mg/L 2,4-D), high concentrations of sucrose (40–50 g/L) and biotin (1.0 mg/L). Embryo maturation and germination was then induced on media containing 0.05 mg/L benzyladenine (BA) and 0.1 mg/L indole-3-acetic acid (IAA). Histological studies of the developing embryos revealed the presence of typical shoot and root poles suggesting that these structures were true somatic embryos. The rate of somatic embryos formation was 500–3,000 per mL settledcell volume while approximately 60% of the embryos regenerated into plants.

Taylor & Bates Brewery & Vinegar Manufactory - Vignette

A vignette of Taylor and Bate's Brewery and Manufactory, St. Catharines.. "Manufacturers of Taylor's Celebrated Amber Ale, Porter, also, White Wine Vinegar, Dealers in Malt, Hops, Barley, &c. &c." The names listed are James Taylor and Thomas B. Bate.

b-Jet identification in the D0 experiment

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for the rare decay B-s(0) -> mu(+)mu(-)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for the Standard Model Higgs Boson in the ZH -> v(v)over-barb(b)over-bar Channel in 5.2 fb(-1) of p(p)over-bar Collisions at root s=1.96 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Search for the Standard Model Higgs Boson in the Missing Energy and Acoplanar b-Jet Topology at root s=1.96 TeV

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Observation of the Doubly Strange b Baryon Omega(-)(b)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Measurement of gamma + b + X and gamma + c + X Production Cross Sections in pp > Collisions at s=1.96 TeV

First measurements of the differential cross sections d(3)sigma/(dp(T)(gamma)dy(gamma)dy(jet)) for the inclusive production of a photon in association with a heavy quark (b, c) jet are presented, covering photon transverse momenta 30 < p(T)(gamma)< 150 GeV, photon rapidities |y(gamma)|< 1.0, jet rapidities |y(jet)|< 0.8, and jet transverse momenta p(T)(jet)> 15 GeV. The results are based on an integrated luminosity of 1 fb(-1) in pp collisions at s=1.96 TeV recorded with the D0 detector at the Fermilab Tevatron Collider. The results are compared with next-to-leading order perturbative QCD predictions.

Search for third generation scalar leptoquarks decaying into Ï„b

We have searched for third generation leptoquarks (LQ3) using 1.05fb-1 of data collected with the D0 detector at the Fermilab Tevatron Collider operating at s=1.96TeV. We set a 95% C.L. lower limit of 210 GeV on the mass of a scalar LQ3 state decaying solely to a b quark and a τ lepton. © 2008 The American Physical Society.

School of Nursing Class of 2006

<b>Top Row: b>Rebecca Adams, Leslie Babich, Katherine Banas, Lori Barnett, Stacey Bednarz, Kelly C Berryman, Adam Brieger, Tina Brown, Kimberly Burleigh, Anne Byrne, Julia Carl, Terra Caswell, Angela Chabot, Molly Colgan, Desiree Conyers, Amy Cook, Melissa Cooley, Ashley Cooper, Morgan Cornell

<b>Row 2: b>Delphine Cornet, Laura Cortina, Casey Cox, Bradley Crow, Lauren D'Agostino, Katelyn Davis, Kara Dendrinos, Rachael Dunckel, Carolyn Ellis, Kristin Ellis

<b>Row 3: b>Deonna French, Erin Gasser, Amanda George, Michelle Gilmore, Jacquelene Goyett, LaRonda Gracia, Tera Greenberg, Tracy Guzzardo, Amy Hamlin Tapper, Shawn Hathaway

<b>Row 4: b>Jennifer Heller, Michele Hetfield, Hilary Heuer, Christen Hicks, unknown, Melissa Jenkins, Terri Jobkar, Jennifer Keller, Karissa Kerg, Katherine Kern

<b>Row 5: b>Keri Kingma, Amanda Kristofik, Brigid Kutner, Melissa LaDuke, Lorraine Law, Katherine Lawler, Allison Ledtke, Corinne Lee

<b>Row 6: b>Kerrie Lemerand, Kristen Maki, Smith Margaret, Cynthia Mathew, Thomas Mazzocco, Cara McAlpin

<b>Row 7: b>Lana McCarthy, Erin McKeever, Nicolyn Meek, Patricia Coleman-Burns, Carol Loveland-Cherry, Judith Lynch-Sauer, Ada Sue Hinshaw, Barbara Guthrie, Marge Calarco, Carolyn Sampeselle, Joanne Pohl, Therese Messing, Rachel Milkowski, Renee Miller

<b>Row 8: b>Andrea S Miller, Stephanie Mizer, Melissa Morgan, Heather Bidgoli, Elisa Brunetto, Jessica Cleghorn, Jade Curry, Ashley Dorow, Megan Finn, Lisa Gruen, Margaret Kelemen, Andrea Munger, Elizabeth Spencer, Mary Vanderweele, Abigail Vertalka, Jackelyn Ng, Phuong Nguyen, Gracia Nicolaescu

<b>Row 9: b>Laura Norris, Elizabeth Osborn, Lavinia Pacurar, Carly Palmer, Kristine Parish, Jill Patterson, Mary Pepper, David Perout, Michael Pfeifer, Kristin Phillips, Susanne Pickman, Vanessa Polly, Sabrina Porter, Christina Quillan, Lauren Ramoie, Natasha Rivers, Teresa Roberts, Megan Robertson, Byanqa Robinson

<b>Row 10: b>Mary Rodzik, Kimberly Sanders, Weber Sasha, Rebecca Scheiblauer, Taylor Schmidt, Jacquelyn Schrot, Tanya Shisler, Daniel Shivel, Sophia Shyu, Michelle Skurulsky, Melissa Smalligan, Erin Sorensen, Allison Spinweber, Lindsay Steiger, Natalya Stokely, Karen Stoneburner, Katherine Stout, Stephanie Swihart, Aaron Taylor

<b>Row 11: b>Lori Thome, Christopher Thuer, Carolyn Trabka, Kathryn Trommbley, Valerie Tumbleson, Stacey Ventola, Dana Verkade, Caitlyn Vert, Angela Videto, Kari Wanless, Abby Wegener, Stephanie Westphal, Eric Williams, Whitney Zachritz, Amber Zemer, Joanna Zizzo, Chelsea Zussman

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

Callus was initiated in three different ‘‘esculenta’’ taro cultivars by culturing corm slices in the dark on half-strength MS medium supplemented with 2.0 mg/l 2,4- dichlorophenoxyacetic acid (2,4-D) for 20 days followed by subculture of all corm slices to half-strength MS medium containing 1.0 mg/l thidiazuron (TDZ). Depending on the cultivar, 20–30% of corm slices produced compact, yellow, nodular callus on media containing TDZ. Histological studies revealed the presence of typical embryogenic cells which were small, isodiametric with dense cytoplasms. Somatic embryos formed when callus was transferred to hormone-free medium and *72% of the embryos germinated into plantlets on this medium. Simultaneous formation of roots and shoots during germination, and the presence of shoot and root poles revealed by histology, confirmed that these structures were true somatic embryos. Plants derived from somatic embryos appeared phenotypically normal following 2 months growth in a glasshouse. This method is a significant advance on those previously reported for the esculenta cultivars of taro due to its efficiency and reproducibility.

Improving taro (Colocasia esculenta var. esculenta) production using biotechnological approaches

Taro (Colocasia esculenta L. Schott) is an important crop worldwide but is of particular significance in many Pacific Island countries where it forms part of the staple diet and serves as an export commodity. Escalating pest and disease problems are jeopardizing taro production with serious implications to food security and trade. Biotechnological approaches to addressing pest and disease problems, such as somatic embryogenesis and transgenesis, are potentially viable options. However, despite biotechnological advancements in higher profile agronomic crops, such progress in relation to Colocasia esculenta var. esculenta has been slow. This paper reviews taro biology, highlights the cultural and economic significance of taro in Pacific Island countries and discusses the progress made towards the molecular breeding of this important crop to date.

Factors affecting somatic embryogenesis and transformation in modern plant breeding

Somatic embryogenesis and transformation systems are indispensable modern plant breeding components since they provide an alternative platform to develop control strategies against the plethora of pests and diseases affecting many agronomic crops. This review discusses some of the factors affecting somatic embryogenesis and transformation, highlights the advantages and limitations of these systems and explores these systems as breeding tools for the development of crops with improved agronomic traits. The regeneration of non-chimeric transgenic crops through somatic embryogenesis with introduced disease and pest-resistant genes for instance, would be of significant benefit to growers worldwide.

Longitudinal cohort study of horse owners

This report summarises the findings of a three-year mixed methods research study designed to capture factors that influence horse owner Hendra virus (HeV) risk mitigation practices. The research project focuses on horse owners; their knowledge, attitudes, and risk mitigation practices, i.e. uptake of vaccination, property management, and biosecurity practices. A flexible research methodology enabled the tracking of core subject areas over time whilst also responding to new or evolving shifts in the HeV landscape, e.g. new HeV cases, event management, and issues arising in the vaccine roll-out. By tracking relationships within the data and engaging with stakeholders and the horse owner population, it is hoped that findings from the study will help to identify important linkages and effective strategies for communication/information and policy implementation.