Cravo-de-defunto como planta atrativa para tripes em cultivo protegido de melão orgânico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estabelecimento de metodologia para alimentação de Aedes aegypti (Diptera-Culicidae) em camundongos swiss e avaliação da toxicidade e do efeito residual do óleo essencial de Tagetes minuta L (Asteraceae) em populações de Aedes aegypti

Objetivou-se desenvolver um procedimento de alimentação de fêmeas de Aedes aegypti que não cause estresse em camundongo swiss e avaliar a toxicidade e o efeito residual do óleo essencial de Tagetes minuta L (Asteraceae) em populações de Aedes aegypti. Camundongos anestesiados: um observado tempo de sedação e outro colocado em gaiola para alimentação de fêmeas. Óleo essencial, diluído em acetona, foi utilizado em bioensaios para avaliação das concentrações letais em larvas de Bauru, SP e São José do Rio Preto, SP, respectivamente, sensíveis e resistentes ao temephos. Os dados obtidos foram comparados com a cepa Rockefeller-EUA. O procedimento com camundongos foi aprovado. Não houve diferença entre as populações quanto à susceptibilidade a Tagetes minuta e os ensaios demonstraram CL50 de 0,24, 0,25 e 0,21mL L-1 e CL99,9 em 0,35, 0,39 e 0,42mL L-1, respectivamente, para Rockfeller, Bauru e São José do Rio Preto. Não foi observado efeito residual da solução.

Etablierung von Kallus- und Suspensionskulturen bei Tagetes erecta L.

Diese Arbeit soll im Rahmen eines kooperationsforschungsvorhabens des Julius Kühn-Institutes in Quedlinburg und der Technischen Universität Wien einen Beitrag zur Aufklärung des Syntheseweges von gelben Flavonolen in der Pflanzengattung Tagetes leisten. Angesiedelt ist dieses Forschungsvorhaben im Institut für Züchtungsforschung an gartenbaulichen Kulturen (JKI) und im Institut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften (TU Wien). Im Mittelpunkt steht dabei die Aufklärung des Syntheseweges von gelben Flavonolen (Halbwirth et al., 2004). Innerhalb dieses Synthesewegs ist die Hydroxylierung von Quercetin zu Quercetagetin durch das Enzym Flavonol-6-hydroxylase (F6H) von besonderem Interesse. Die für die F6H kodierenden Gene könnten zum Beispiel anhand einer Pflanze lokalisiert werden, die aufgrund einer Mutation keine F6H-Aktivität aufweist. Bisher wurden keine derartigen Mutanten bei Tagetes gefunden. Für Mutationsversuche zur Klärung von Fragen der Biosynthese oder des Abbaus von sekundären Pflanzenstoffen sind Gewebekulturen besonders geeignet (Heß, 1992). Für die weitere Forschung sollen im Rahmen dieser Arbeit Kalluskulturen von den Tagetes erecta-Sorten „Antigua“ und „Bianca“ etabliert werden. Sieben Kulturmedien und vier Explantattypen wurden von der Autorin ausgewählt und sollen hinsichtlich der Kallusinduktion getestet werden. Der Einfluss von drei Belichtungsvarianten auf die Kallusinduktion wird untersucht. Der etablierte Kallus soll in Suspension überführt werden. Die Suspensionskulturen können für molekularbiologische Arbeiten, Mutationsversuche sowie Versuche zur Flavonolsynthese verwendet werden. (...)

Ocorrência e recomendações para o manejo de Sirex noctilio Fabricius (Hymenoptera, Siricidae) em plantios de Pinus patula (Pinaceae) em Minas Gerais, Brasil

A vespa-da-madeira Sirex noctilio Fabricius, 1793 (Hymenoptera, Siricidae) é a praga mais importante das florestas cultivadas com Pinus spp. no Brasil. Foi introduzida no Rio Grande do Sul em 1988 e sua dispersão ficou restrita aos estados do sul do país até 2004, quando foi detectada em São Paulo. Neste trabalho é relatada a ocorrência de S. noctilio em plantios de Pinus patula em Minas Gerais, em janeiro de 2005. São discutidas algumas medidas que podem ser adotadas para restringir a dispersão de S. noctilio na região Sudeste.

Antioxidant activities and lutein content of 11 marigold cultivars (Tagetes spp.) grown in Thailand

Antioxidant activities and total phenolic content (TPC) were analyzed in ethanol extracts of 11 marigold cultivars grown in Thailand. Antioxidant activity assays performed in this study were the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity, ferric ion reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, oxygen radical absorbance activity (ORAC), and superoxide anion radical scavenging activity (SRSA) assays. ‘Optiva Orange’ showed the best activity in the ORAC assay and in % SRSA, as well as the highest content of lutein (20.59 mg), gallic acid (25.77 mg), and quercetin (12.61 mg) per gram dry marigold petal among the 11 cultivars. Furthermore, ‘Optiva Orange’ showed the highest lutein yield per plant, as compared to other cultivars. In contrast, ‘Rodeo Gold’ showed the highest activity by ABTS testing (0.92 mmol of trolox/g dry sample), as well as an 89.90% inhibition of DPPH. Lutein content showed a positive correlation with TPC and all antioxidant activity assays. In conclusion, ‘Optiva Orange’ and ‘Rodeo Gold’ could be utilized as a good lutein source for functional food products and cosmetics.

Efectos de pigmentación en el músculo de trucha arcoiris (Oncorhynchus mykiss) mediante el uso de extractos pigmentantes de flor de cempasúchil (Tagetes erecta) y paprika (Capsicum annuum) incorporados en el alimento balanceado.

Tesis (Maestría en Ciencias con Especialidad en Ecología Acuática y Pesca) UANL

Identificación de compuestos activos en dos especies de Tagetes (Compositae) con toxicidad para larvas de Aedes Aegypti L.

Tesis (Maestría en Ciencias con Especialidad en Entomología Médica) UANL

Evaluación de crecimiento y productividad de Pinus patula Schl. et Cham., en la región de Huayacocotla, Veracruz, México [por] Carlos Rutilio Monroy Rivera

Tesis (Maestría en Ciencias Forestales) U.A.N.L.

Aspectos ecológicos y parámetros poblacionales en el caracol de tinte Plicopúrpura patula pansa (GOULD, 1853), en el litoral rocoso del Estado de Guerrero, México

[Tesis] (Doctor en Ciencias Biológicas con Especialidad en Ecología) U.A.N.L.

Patrón espacial y gradiente vertical del caracol de tinte Plicopurpura patula pansa (GOULD, 1853), en la costa rocosa del Estado de Guerrero, México

[Tesis] (Doctor en Ciencias Biológicas con Especialidad en Ecología) U.A.N.L.

Isolation, Stabilization and Characterization of Xanthophyll from Marigold Flower- Tagetes Erecta-L

The present paper deals with the chemistry, isolation, separation, characterisation and stabilisation of the Marigold oleoresin and its application as a natural food colorant. Marigold (Tagetes Erecta L), an ornamental plant belonging to the composite family, has a rich source of natural antioxidant-Lutein. A natural pigment, xanthophylls offer an alternative to synthetic dyes as a food colorant, due to its non-toxicity. Chromatographic separations of saponified and unsaponified oleoresin were performed and Trans-Lutein identified as the major constituent. Well-preserved flowers exhibit a high yield of Xanthophyll content (105.19 g/Kg) in contrast to the unpreserved flower sample (54.87 g/Kg), emphasizing the significance of flower preservation in the extraction of xanthophyll. The stability and amount of xanthophyll also increased from 105.19 g/Kg to 226.88 g/Kg on saponification and subsequent purification with Ethylene Dichloride

Marigold (Tagetes erecta L.) as an attractive crop to natural enemies in onion fields

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Herbicide selectivity in herbaceous ornamental gardens of tagetes erecta L.

One of the major problems in landscaping in tropics is weed management. The herbaceous ornamental plant, Tagetes erecta L. (Asteraceae), is very popular for its beautiful flowers which can be used in landscape and also as cut flowers. The increase use of this plant and lack of selective herbicides led to the objective of this study to evaluate the herbicide metribuzin selectivity for this ornamental plant. The experimental design was completely randomized using four treatments (herbicide metribuzin doses: 0, 0.5, 1.0 and 1.5 L ha-1, equivalent to 0, 240, 480 and 720 g ha-1) and five replicates. The herbicide was applied over the seedling of T. erecta as pre-emergence of the weed seeds. Evaluations were performed at 7, 14, 21 and 28 days after application (DAA) by visual analysis of the toxicity symptoms over the ornamental, using the European Weed Research Council (EWRC) scale (1 to 9), where 1 is the total absence of symptoms and 9 to death plants. Polynomial regression statistical analysis was used. It was verified that, from the dose of 1.0 L ha-1, T. erecta plants died by 14 DAA, which the most of them had presented a very strong toxicity symptom; for the 0.5 L ha-1 treatment the plants had survived until 28 DAA. However, most of them already exhibited the high toxicity level, dying at 35 DAA. Thus, metribuzin was not suitable for T. erecta even at the lowest rate tested in this study.

Caracterização do saldo de radiação em ambiente protegido e a campo de seus efeitos sobre as necessidades hídricas do cravo-de-defunto (Tagetes sp.)

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Seletividade do herbicida oxyfluorfen para as plantas ornamentais Dianthus chinensis L. e Tagetes erecta L

Pós-graduação em Agronomia - FEIS