Somatic embryogenesis from ovaries of sweet orange cv. Tobias

Callogenesis, somatic embryogenesis, and regeneration were obtained from tissues of unfertilized ovaries of sweet orange (Citrus sinensis Osbeck.) cv. Tobias. The influence of two modified basal media, woody plant medium (WPM) and N6 medium, to induce callus formation from pistils was determined. Overall, high frequencies of callogenesis were observed when either medium was used. However, initial culture of explants in WPM medium followed by transfer of callus to N6 medium resulted in higher frequency of callus induction (of 2.30 callus per explant that were larger than 0.5 cm in size), and of subsequent development of embryogenic callus (10%). A total of 125 somatic embryos were obtained. After 6 months of culture, 72% of somatic embryos germinated into plantlets. These plantlets were subsequently micrografted in vitro, and then acclimatized. Ploidy of these plants were determined using flow cytometry and TRAPS molecular markers were used to confirm their maternal origin.

Die Söhne des Tobias, die Hellenisten und der Spruchdichter Sirach

Heinrich Graetz

Das Buch Tobias oder Tobit, seine Ursprache, seine Abfassungszeit und Tendenz

Heinrich Graetz

Das Buch Tobias oder Tobit, seine Ursprache, seine Abfassungszeit und Tendenz

Heinrich Graetz

Das Buch Tobias oder Tobit, seine Ursprache, seine Abfassungszeit und Tendenz

Heinrich Graetz

Das Buch Tobias oder Tobit, seine Ursprache, seine Abfassungszeit und Tendenz

Heinrich Graetz

Tobias Levy, 1851-1917 : (Aus: The Defeated [1927])

P. Arnold

Tobias Jakovits: Die Judenabzeichen in Böhmen [Rezension]

H.

Heinz Geldern, Szenenfoto, Brustbild, in: Hermann Heinz Ortner: Tobias Wunderlich

Signatur des Originals: S 36/F08628

Tobias Matthay, Brustbild

Signatur des Originals: S 36/F09229