Increased attractiveness of honeybee hive product volatiles to adult small hive beetle, Aethina tumida, resulting from small hive beetle larval infestation

The small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae), is a recent but significant pest of honeybee Apis mellifera L. (Hymenoptera: Apidae) hives in various regions throughout the world, including Eastern Australia. The larval stage of this beetle damages hives when they feed on brood, pollen, and honeycomb, leaving behind fermented wastes. In cases of extreme damage, hives collapse and are turned to an odorous mass of larvae in fermenting hive products. The yeast Kodamaea ohmeri (Etchells & Bell) Yamada et al. (Ascomycota) has been consistently isolated from the fermenting material as well as each life stage of this beetle. Various studies have noted that the small hive beetle is attracted to volatiles from hive products and those of the yeast K. ohmeri, although earlier studies have not used naturally occurring hive products as their source of fermentation. This study investigated changes through time in the attractiveness of natural honeybee hive products to the small hive beetle as the hive products were altered by the action of beetle larvae and fermentation by K. ohmeri. We used gas chromatography-mass spectrometry and choice-test behavioural assays to investigate these changes using products sampled from three apiaries. Attractiveness of the fermenting hive products (‘slime’) increased as fermentation progressed, and volatile profiles became more complex. Fermenting hive products remained extremely attractive for more than 30 days, significantly longer than previous reports. These results have strong implications for the development of an external attractant trap to assist in the management of this invasive pest.

Evaluation of tomato production systems at Bowen in mitigating the risk of fruit fly infestation: a systems approach for market access

Queensland fruit flies Bactrocera tryoni and B. neohumeralis are considered major quarantine pests of tomato, a major crop in the horticultural production district around Bowen, North Queensland, Australia. Preharvest and/or postharvest treatments are required to meet the market access requirements of both domestic and international trading partners. The suspension from use of dimethoate and fenthion, the two insecticides used for fruit fly control, has resulted in the loss of both pre and postharvest uses in fresh tomato. Research undertaken quantitatively at Bowen evaluated the effectiveness of pre-harvest production systems without specific fruit fly controls and postharvest mitigation measures in reducing the risk of fruit fly infestation in tomato. A district-wide trapping using cue-lure baited traps was undertaken to determine fruit fly seasonal patterns in relation to the cropping seasons. A total of 17,626 field-harvested and 11,755 pack-house tomatoes were sampled from ten farms over three cropping seasons (2006-2009). The fruit were incubated and examined for fruit fly infestation. No fruit fly infested fruit were recorded over the three seasons in either the field or the pack-house samples. Statistical analyses showed that upper infestation levels were extremely low (between 0.025 and 0.062%) at the 95% confidence level. The trap catches showed a seasonal pattern in fruit fly activity, with low numbers during the autumn and winter months, rising slightly in spring and peaking in summer. This seasonal pattern was similar over the four seasons. The main two species of fruit fly caught were B. tryoni and B. neohumeralis. Based on the results, it is clear that the risk of fruit fly infestation is extremely low under the current production systems in the Bowen region.

A comparative assessment of the methods of control of water hyacinth infestation with regards to fish production

Water hyacinth (Eichhornia crassipes) has been subject of three control methods since its arrival into the Nigerian freshwater lagoon system in 1984 - mechanical, chemical and biological. An assessment of these three methods seems to suggest that mechanical and chemical control methods, both of which being costly, must be applied either solely or integrated to combat the present level of considerable infestation in Nigeria. The biological control methods are advisable for slow, sustained control and can only cope with low levels of infestation. It is thus concluded that the preliminary control method should be mechanical or chemical to effectively abate the nuisance plant, followed by biological control once infestation levels have been sufficiently reduced

Incidence of mayfly (Povilla sp.) infestation of wooden infrastructures of artisanal fishers in the lagoon and marine fisheries locations of Ogun State, Nigeria

A diagnostic survey of the incidence of mayfly (Povilla sp.) infestation of wooden infrastructures of the artisanal fishers in ten (10) lagoons and marine fishing villages of Ogun States (Nigeria) was carried out through the application of structured questionnaire and participatory Rural Appraisal interviews. The demographic, infrastructural and socioeconomic characteristics of the ten fishing villages sampled were derived and analyzed. The infestation which occurs all year round is found to be most prevalent (70%) in the wet season, increasing proportionally with salinity from 56% (brackish water); to 63% (marine water). The life-span of Povilla sp. is reduced from 55% to 62% (freshwater); 41% (brackish water) and 38% (marine water). Annual financial loss of N10,000.00 per fisher or N80,000,000.00 to the 8000 artisanal fishers affected in Ogun State is discussed. It is recommended that fishers should preferably use non-wood crafts and infrastructures while adopting appropriate management strategies for containing the existing infestation

Control of insect infestation in smoked West African sardines Sardinella maderensis 'Sawa' using actellic 50 EC solution

Fish which has been cured or are in the process of being cured by traditional methods are usually infested by insects, posing a real problem to traders and processors, especially in Nigeria. The effects of 0.03% actellic 50 EC solution and vegetable oil on insect infestion were studied using West African sardines, Sardinella maderensis. Actellic solution was more effective in combating insect infestation than vegetable oil. Appearance and perceived smoked fish flavour of fish treated with Actellic and vegetable oil differed (P<0.05), while taste was unaffected by treatment. Actellic 50 EC solution though effective, could be subject to abuse

Lake Kainji water hyacinth infestation and the control strategies

The paper documents the aquatic vascular plants situation of Lake Kainji, particularly with the invasion of water hyacinth (Eichhormia crassipes) hitherto alien to the lake system. The frequency of occurrence of some aquatic plants on Lake Kainji have increased whilst a few decreased over the period between 1984 and 1995. More than 53.6% of the lake surface has now been covered by aquatic vascular plants of which water hyacinth alone covered 30.6% (16.4% of the system). Apart from the characteristic rapid multiplication and biomass build-up of the plant, more than 9.1 clumps with a diameter range between 0.8-4cm enter the lake per minute. Thus more than 16.4ha per day or annually 6000ha of water hyacinth are added as drifts. Because of the peculiar hydrological cycle of the lake and the plant aggressiveness and invasiveness, NIFFR (with the support of the GTZ) had developed an approach of control which combines manual, biological, natural, boom construction and chemical measures. These are discussed

Survey of fungal infestation of some fish species from Tagwai dam, Minna, Niger State

Survey of Fungal infestation of some species of fish in Tagwai Dam Minna was carried out from March to June 2002. Fungi were isolated from the scale/skin, gills and fins. Twenty-one fungi species were identified from 18 species of fish microbial growth was measured by direct cell count using Stuart colony counter. Most of the fungi encountered were of the mould group and infestation occurred among all the species sampled. The infestation was predominantly by Aspergillus species and the scale/skin was most widely affected. The study showed the identified fungi in order of frequency to be as follows. Aspergillius niger. Rrhizopus spp. Mmucor spp Aspergilus flavum. Aspergillus parasitous. Aspergillus fumigatus, Microsporum canis. Penicillum virridicalumand Fusarium spp respectively. Aspergillus niger occurred on all the species of fish sampled. Barrilius spa and Chrysichrhy auratus longifilis had significantly higher (P<0.05) mean fungal load on their fins and gills. There was no significant difference (P>0.05) in the mean fungal load in different parts of the body of other fish species sampled

Botanical insecticides prescription for fish pest control and infestation free protein yield

The dry powders of four local species namely Piper guineense Schum and Thonn, Aframomum melegueta Schum, Zingiber officinale Rose; Capsicum annum Miller at three concentrations of 15g, 20g and 25g.kg were evaluated for their insecticidal effects against the larval of the dried fish weevil Demestes maculates Degeer. All the four species showed some effectiveness with P. guineense given a 100% mortality at the end of 72 hours at the three concentrations. The other species though gave less mortality were able to slow down the rate of development of the larvae to the adult size

Quantifying gregarine infestation of Penaeus vannamei on a commercial shrimp farm and some attempts at treatment

The gregarine (Nematopsis spp.) infestation in Penaeus vannamei on a commercial shrimp pond is discussed focusing on quantifying the parasites and some attempts to control infestation.

A new technique to control insect infestation in Mas min

A process of heat treating Mas min at 125°C for 15 minutes and hermetically packing them up has been investigated. This method has been found to be successful in controlling insect infestation in Mas min. The Japanese method of packing Mas min in BHC coated containers has also been simultaneously examined and found successful under certain conditions. However, this process is found to impart slight undesirable flavors to the product. In addition, this chemical treatment is also likely to be more costly.

Status of water hyacinth infestation and control in River Kagera

During a regional workshop held in Mukono, Uganda (May 2001) by scientists and technocrats from Kenya, Tanzania and Uganda, working on water hyacinth management under the Lake Victoria Environmental Management Project (LVEMP), it was resolved that a survey of River Kagera be made to study the status of water hyacinth infestation and biological control in the river. Reports at the Mukono Workshop indicated that although Tanzania and Uganda had made serious effort to introduced biological control weevils (Neochefina eichhorniae and Neochetina brucht) on the weed in River Kagera, the level of establishment of biological control in the river was doubtful. Large quantities of water hyacinth biomass drifted down River Kagera into Lake Victoria daily. Similar reports of apparent inability of biological control weevils to fully establish and have effect on water hyacinth in River Nile, especially the Upper Victoria Nile, were also made by Uganda, and large quantities of weed biomass continuously drifted down the Upper Victoria Nile into Lake Kyoga. This was in spite of the successful control of the weed in Lake Victoria between 1998 and 2000

A survey of pest (Dermestes maculatus) infestation of dried preserved Clarias gariepinus: its effect on nutrient quantity and quality

Studies on the nutrient composition of smoked-dried Clarias gariepinus were carried out, to assess the effects of pest infestation on the dried catfish from two different market (Abakpa and Kpirikpiri market) area in Abakaliki Metropolis, Ebonyi State, Nigeria. Fish samples from the two markets where kept for pest to infest it and it were taken to Biochemistry Laboratory of Food Science and Technology in Ebonyi State University to assess the damage of pest on dried catfish using standard methods. There was a significant different among the mean weight of fish from the two markets (Abakpa and kpirikpiri; 19.13kg and 18.98kg respectively) compared with the mean weight of the control 20.09kg. There was a variation in the proximate composition of the fishes from the two markets compared with the control, the decreases of the proximate composition are: crude protein from 60.07% to 40.27%; fat from 8.35% to 5.90%; moisture content from 11.67% to 10.67; fiber from 12.93 to 11.93; ash from 4.02% to 3.54% and carbohydrate 12.67% to 5.89%. The longer the storage periods of the infested smoked fish the more the tissue was degraded. Proper handling and storage of dried catfish are required to prolong the shelf life and nutrient content of dried catfish.

Prevention of infestation by Dermestes maculatus Degeer in east African dried fish by solar treatment

East African sun-dried fish infested by Dermestes maculatus were exposed to tropical sunlight at ambient temperature and analysed for insect mortality and weight losses. Solar treatment for 6 to 8 lo was highly effective for one layer of split sun-dried fish and 100% insect mortality was toted, while pest species were still present in the four layer batch. Weight losses between 1.2% and 10.2% were recorded, the top layer suffering the highest loss. The high surface temperature of 60°C caused fish to become brittle and quality losses occurred. A reduction in length of exposure/temperature is probably a presupposition for application of the method to local conditions.

Prevention of infestation by Dermestes maculatus Degeer in east African dried fish using pyrethrum and piperonyl butoxide

East African sun-dried fish dipped for 4 seconds in different solutions of pyrethrum and piperonyl butoxide were analysed for insecticide residue limits. All analyses showed residues above the FAO/WHO MRL; exceeding factors of between 7.6 (22.9 ppm) and 1.6 (5.3 ppm) were found for pyrethrum while exceeding factors between 5.1 (102 ppm) and 1.7 (33.1 ppm) were common for piperonyl butoxide after 6 months storage at ambient temperature. All insecticide treated fish, regardless of dip concentration, were observed to be less susceptible to infestation by Dermestes maculatus than samples of untreated fish. No dry weight losses due to insect infestation were recorded, however moisture evaporation caused weight losses between 6 and 8% during the period. Further investigations showed that careful handling and a dip concentration more in accordance with FAO/WHO MRL than the commercial practice will reduce the cost of insecticides from K.sh. 0.72 to K.sh. 0.23 per kg pyrethrum treated fish.