Influence of the colony cycle on physiological and morphological caste variation in the perennial neotropical swarm-founding social wasp, Protonectarina sylveirae (Hymenoptera, Vespidae, Epiponini)

This paper describes the influence of the colony cycle on caste differences in Protonectarina sylveirae. Despite invariably keeping the main aspects of the conspicuous dimorphism between the castes, it is shown that: 1- queen-worker differences varied according to the progress of the colony cycle, 2- queens in pre-emergence colonies were smaller than those in male-producing ones, 3- workers were larger in pre-emergence than in other colony stages, 4- at particular steps of the colony cycle, non-inseminated ovary-developed queens and workers were detected. The size of the spermatheca differed between inseminated and non-inseminated queens with the highest values appearing in those bearing the most developed kind of ovary development. Differences between inseminated and non-inseminated queens were found in tergites III and IV, ovarian development and amount of fatty tissues. Because tergite size relates to gaster size, this character may be an important stimulus for selection of larger queens in the course of the colony cycle.

Morphological caste studies in the neotropical swarm-founding polistinae wasp Angiopolybia pattern (Lepeletier) (hymenoptera: Vespidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

More than one inseminated female in colonies of the independent-founding wasp Mischocyttarus cassununga von Ihering (Hymenoptera, Vespidae)

In the primitively eusocial wasps, especially Polistini and Mischocyttarini tribes, the physiological condition of each individual is strongly associated with its dominance status in the colonial hierarchy. As a rule, in independent-founding wasps, female wasps are all morphologically alike, and their role is apparently quite flexible even as adults. However, some studies have shown that differences in body size can exist between reproductive and non-reproductive females. Thus, the present study aimed at detecting differences between reproductive (inseminated) and non-reproductive (uninseminated) individuals based on morphological and physiological parameters. We tape-recorded the daily behavioural repertory of six colonies of Mischocyttarus cassununga for determining the hierarchical dominance in the field, and then collected these colonies (in different cycle stages) for measuring 13 set characters, and assessing the physiological condition of each individual by inspecting their fat bodies and ovaries. Our results revealed that inseminated and uninseminated females are not significantly different in relation to body size, in spite of first group shows higher average than second in almost all measured parts. The physiological evaluation of each individual demonstrated more than one inseminated female per colony during all stages of the colony cycle, suggesting a strategic condition of this species against difficulties (predation and parasitism of the colony) in nature.

More than one inseminated female in colonies of the independent-founding wasp Mischocyttarus cassununga von Ihering (Hymenoptera, Vespidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Overview of Caste Differentiation in the Polistinae, Emphasizing the Neotropical Swarm-Founding Polistinae (Hymenoptera, Vespidae, Epiponini)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evolution of caste in neotropical swarm-founding wasps (Hymenoptera : Vespidae; Epiponini)

Reproductive castes are compared in species of swarming wasps representing all currently recognized genera of Epiponini (Polistinae). New morphometric data for nine measures of body parts and ovarian data are presented for 13 species. These are integrated with all similarly conducted available studies, giving a total of 30 species. Analysis reveals several syndromes relating reproductive and nonreproductive individuals: no meaningful distinction, physiological differences only, reproductives larger than nonreproductives with intermediate individuals present, reproductives different in shape from nonreproductives with no intermediates, and reproductives smaller in some aspects than nonreproductives. Distribution of these syndromes among species is consistent with phylogenetic relationships derived from other data. Optimizing these syndromes on the cladogram indicates that the basal condition of Epiponini is a casteless society that is not comparable to the primitively social genus Polistes where dominant queens control reproduction. Castes originate several times in Epiponini, with different results in different lineages. The best documented evolutionary sequence passes from casteless societies, to those with reproductives larger, to those with reproductives differing in shape from nonreproductives, to those with reproductives smaller in some measures. This sequence is consistent with Wheeler's theory of the origin of caste through developmental switches, and represents the most thorough test of that theory to date.

Division of labor in stable social hierarchy of the independent-founding wasp Mischocyttarus (Monocyttarus) cassununga, von Ihering (Hymenoptera, Vespidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Marimbondos: a review on the neotropical swarm-founding polistines

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Application of Microsatellite Primers Developed for Polistes in the Independent-Founding Wasp Polists satan Bequaert (Hymenoptera: Vespidae)

Microsatellite primers developed for a given species are sometimes useful for another in the same genus and in other genera within the same family, making possible to search for pre-existing suitable primers in the databanks such as GenBank. We examined whether existing primers developed for Polistes could be used for Polistes satan Bequaert. We tested 50 microsatellite primers from three Polistes species and found that six microsatellite loci show polymorphism in size in P. satan. These six loci were highly polymorphic, having four to 15 alleles in P. satan with an expected heterozygosity of 0.525-0.832. These loci can be used to study parameters concerning genetic relatedness such as social interactions in colonies and genetic conflicts of interest among nestmate individuals.

Isolation and characterisation of microsatellite loci in the wasp Mischocyttarus cassununga

Mischocyttarus cassununga, a primitively eusocial Brazilian wasp, commonly found in urban environments, is an interesting model for studies on the evolution of social behaviour in hymenopteran insects. In this study, we constructed a microsatellite-enriched genomic library and presented primers for 18 microsatellite loci. For the analysis, 20 unrelated females were screened and all loci obtained were polymorphic. PCR amplification revealed from 3 (Mcas5b) to 17 (Mcas53b) alleles per locus (). We detected the levels of observed (H (o)) and expected (H (e)) heterozygosities ranging from 0.150 to 0.950 and 0.261 to 0.920, respectively. The polymorphic information content ranged from 0.238 to 0.915, averaging 0.680. All loci were in Hardy-Weinberg equilibrium and linkage disequilibrium was not detected after sequential Bonferroni correction (P > 0.05). These molecular markers will allow further studies on sociogenetic structure, extensive population genetic analysis and diversity of M. cassununga and other Mischocyttarini species.

Necrophagy by the social wasp Agelaia pallipes (Hymenoptera : Vespidae, Epiponini): Possible forensic implications

The occurrence of 62 specimens of Agelaia pallipes caught in carrion traps using three types of baits (fish, cow liver and poultry viscera) in three different types of environments (rural, urban and forest area) in seven municipalities in Southeastern Brazil is reported here. This specific necrophagic behavior is discussed, since investigations concerning carrion wasps are scant in literature.

Morphology of Richards' Gland in the Wasp Metapolybia docilis

Richards gland in the epiponine wasp Metapolybia docilis occurs at the anterior side of the 5(th) abdominal sternite, and is formed by approx. 360 secretory cells. The cells discharge their secretory products through accompanying duct cells into a reservoir that is formed by the invaginated intersegmental membrane between the 4(th) and 5(th) sternites. The ultrastructural characteristics of the secretory cells are indicative for the production of a non-proteinaceous secretion, which is in line with the trail substance that is used by these wasps during their swarm-founding.

An adaptive view of caste differentiation in the neotropical wasp Polybia (Trichothorax) sericea Olivier (Hymenoptera: Vespidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cladistic analysis of Charterginus Fox, 1898 (Hymenoptera, Vespidae, Epiponini). A neotropical genus of social wasps

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Castes and asynchronous colony cycle in Polybia bistriata (Fabricius) (Hymenoptera: Vespidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)