Study on the biomass yield of Duckweed (Lemna minor) (L.) in hydroponic cultures containing different concentrations of aquaeous extract of chicken manure

The biomass yields of duck week (Lemna minor(L) was monitored in hydroponic media prepared by variously extracting 0.50, 1.00 and 2.00g of dried chicken manure per liter of city water (tap water) supply. The culture media consisting of aqueous extract of the various manure treatments were made up to 12 liters in all cases with tap water as control. Plastic baths of 25 liters capacity with 0.71 super(m2) surface area were used as culture facility. Each bath was stocked at a density of 30g super(m-2) with fresh weed samples (i.e 21.30g/bath). Maximum yields were obtained at all treatment levels and control on day 3 and based on the highest yield of 0.37gm super(-2)d super(-1) (dry matter) obtained at 1.00gL manure treatment which was however not significantly higher (P>0.05) than the 0.36gm super(-2)d super(-1) (dry matter) at 0.05gl super(-1) media manure content, an average manure level of 0.75l super(-1) was selected and used to determine the operational plant density. Thus fresh weights of 30 to 300gm super(-2) was grown in triplicate at 30g intervals for a period of 3 days. A regression equation of Y=2.6720+0.0021x with a corresponding maximum density or operational plant density of 266gm super(-2) and yield of 0.98gm super(-2), d super(-1) (dry matter) were obtained. Further growth trials were carried out at the operational density and manure levels of 0.50, 0.75, 1.00, 1.25, 1.50, 1.75 and 2.00gl super(-1) media manure concentration giving a significantly higher yield (P<0.05) of 17gm super(-2), d super(-1) (dry matter). This yield was however doubled to between 2.21 and 2.24gm super(-2) d super(-1) (equivalent to 7.96 to 8.06mt.ha-1, Yr-1 dry matter on extrapolation) if 25% and 75% respectively of the total weed cover were harvested daily within the experimental period. The role of some dissolved plant nutrients (DPN) were also discussed

Fecundity estimates for Maryland Striped Bass

Contemporary striped bass population modeling efforts on coastal stocks point to a reduced population fecundity in Chesapeake Bay being partially responsible for declining reproduction (Anonymous 1985; Boreman and Goodyear 1984). Fecundity values used in these models were based on earlier work by jackson and tiller (1952), lewis and Bonner (1966), Hollis (1967) and Holland and Yelverton (1973). An important feature to the Boreman and Goodyear (1985) model (FSIM) is an accurate determination of the fecundity weight regression equation used to determine the rate of egg deposition over time. Egg deposition models in turn can be used to determine how reproductive potential is changing over time in response to various management actions, i.e. reducing fishing mortality rates. thus it is imperative to follow population stock structure in the Bay system and to develop a contemporary fecundity relationship for striped bass. This report deals with the gonadal material collected in 1986 and 1987 from a coordinated Maryland field program. Samples were obtained from drift gill net collections during the spawning season from four localities: Potomac Estuary, Upper Bay, Chesapeake and Delaware Canal, and the Choptank Estuary (Figure 1).

Length-weight relationship and relative condition factor in Macrobrachium (Hellex)

The length-weight relationship was calculated for the freshwater prawn Macrobrachium idae. About 150 specimens of M. idae (males 50, females 50 and 50 juveniles) were utilised for this study. The length-weight relationship was assessed separately for males, females and indeterminants. The regression equation for males, females and indeterminants showed significant differences whereas it was insignificant for males and females. The variations in length-weight relation between sexes and indeterminants were compared and discussed. The relationship between total length with carapace length and total length with rostral length were also determined.

Relationship between total length and mouth gap of some commercially important carp fry

The estimated regression equation for total length and mouth gape computed were Log TL = Log 0.23 + 0.663 log MG (vertically) (r = 0.960) and Log TL = Log 0.08 + 0.686 log MG (horizontally) (r = 0.949). In case of rohu average total length from 11350 mm to 23775 mm and mouth gape 805 um to 1225 um (vertically) and 700 um to 1110 um (horizontally) between the first day of mouth opening up to 15 days. The regression equation for total length and mouth gap were Log TL = Log 0.20 + 0.660 log MG (vertically) (r = 0.935) and Log TL = Log 0.02 + 0698 log MG (horizontally) ( r = 0.907). In case of silver carp average total length from 12800 ,urn to 33555 um and mouth gape 690 um to 1210 um (vertically) and 615 um to 1115 um (horizontally) between the first day of mouth opening up to 15 days. The regression equation for total length and mouth gape were Log TL = Log 0.36 + 0.596 log MG (vertically) (r = 0.936) and Log TL = Log 0.26 + 0.607 log MG (horizontally) (r = 0.891). The relationship between total length and mouth gape (vertically and horizontally) of the studied fry were found to be linear and highly significant.

Morphometric measurements as an index for estimating yield of meat in shell fishes, 2. Mussel (Perna viridis) and clam (villorita cyprinoides)

Observations (76 nos) on height-length and whole weight-meat weight relations of mussels (Perna viridis), both wild and cultured were made. From the length of mussel the height can be worked out by the equations (logarithmic scale), 1. y = 0.360+0.988 x for wild; 2. y = 0.334+1.011 x for cultured, where x is the length (cm) and y is the height (cms). So also to any height the corresponding meat weight can be obtained by the regression equation. log w=-0.8178+1.9769 log H for wild variety (1) log w=-1.3049+2.8385 log H for culture-variety (2) where w is the meat weight (g) and H is the height (cm) of the mussel. Fourteen observations on size weight measurements of dams were made. The yield varied from 8.9 to 13%. The length-height relationship worked out for clams (Villorita sp) is y=0.485+1.005 x for length x and height y.

Acute toxicity of metasystox to wedge clam, Donax cuneatus from west coast

96h acute toxicity tests were performed using commercial grade metasystox on the marine wedge clam, Donax cuneatus during summer 1985. The behaviour and mortality rates were recorded periodically. Most of the dams responded in opening the shell valves and extending the siphons quicker in low test concentrations (0.004-0.0052 p.p.m) but this was slow and late in high concentrations (0.0056-0.008 p.p.m). Mortality began to occur in 0.008 p.p.m. from 12 h, whereas, in 0.0052 p.p.m. from 60 h onwards. The observed LC sub(0) value was 0.004 p.p.m. and LC sub(50) 0.0064 p.p.m. The regression equation established was Y = 79.0891 + 33.4523 X. The rate of oxygen concentration increased at LC sub(0) and LC sub(50) values compared to control indicating the disturbed physiological adjustment. The results are correlated with physico-chemical parameters of seawater and discussed in the light of pesticide toxicity to the dam.

Length-weight relationship and relative condition factor in Daysciaena albida (Cuv.) and Gerres filamentosus (Cuv.)

The length-weight relationship of Daysciaena albida and Gerres filamentosus were calculated separately for indeterminants, mature males and mature females. The logarithmic regression equation obtained for D. albida - males: log w = -1.5055 + 2.8618 log l; females: log w = -0.9260 + 2.4089 log l; indeterminants: log w = -l.7188 + 3.0616 log l. The regression co-efficients between males and females, males and in determinants and female and in determinants showed significant differences. In G. filamentosus the relationship can be expressed as males: log w = -1.3224 + 2.8740 log 1; females: log w = -1.2874 + 2.8381 log l; indeterminants: log w = -0.8167 + 2.2558 log l. The difference in regression co-efficients between male and female are insignificant at 5% level whereas significant differences were observed between males and indeterminants and females and indeterminants. The relative condition factor (Kn) was calculated for the above two species. In D. albida the reasons for the fluctuations of Kn values can be attributed to both spawning cycle as well as feeding intensity whereas in G. filamentosus it synchronies mainly with spawning cycle.

Length-weight relationship in Nandus nandus (Hamilton) (Perciformes-Teleostei)

Length-weight relationship of the freshwater fish of Pamba River, Nandus nandus (Ham) has been worked out. The results showed that the slope values and elevations were not significant and hence a combined regression equation has been calculated for both the sexes (Log W=2.4130 Log L -0.3306). The’t’ test analyses were conducted and found that the growth departs significantly from the isometric growth. Thus the formula W=aL super(n) has to be applied in calculating the length-weight relationship of this species.

Allometric study of skeleton weight, body weight and length relationship of Euryglossa orientalis (Bl. & Schn.) (Family: Soleidae) from Karachi coast

Present study deals with the family Soleidae (common sole) Euryglossa orientalis (Bl. & Schn.) of the order Pleuronectiformis from Karachi coast. Separate equation (regression line) for describing the length weight relationships for male and female combined are justified. Allometric studies were made on skeleton weight relative to the length and the weight of the fish. The regression equation 'a' and 'b' values of standard length/skeleton weight and body weight/skeleton weight are statistically significant.

Attenuation of photosynthetically available radiation by chlorophyll, chromophoric dissolved organic matter, and tripton in lake Donghu, China

The attenuation coefficient of photosynthetically available radiation [K-d(PAR)] and three water quality parameters [chlorophyll a (chl a)], chromophoric dissolved organic matter (CDOM) and tripton] were measured at three stations in shallow, subtropical Lake Donghu from April 2003 to March 2004. The multiple regression equation of K-d(PAR) versus chl a, CDOM, and tripton was: K-d(PAR) = 0.44 + 0.019 chl a + 1.88 CDOM + 0.016 tripton, which revealed the relative contributions of the three parameters to K-d(PAR). The effects of water and CDOM on K-d(PAR) were of minor importance (19-26%), while chl a and tripton were the two greatest contributors, accounting collectively for 74-81%.

ROLE OF PHOSPHORUS CYCLING IN ALGAL METABOLISM AND ALGAL SUCCESSION IN LAKE DONGHU, CHINA

The role of phosphorus cycling in algal metabolism was studied in a shallow lake, Donghu, in Wuhan using the methods of measuring cell quota C, N and P, and calculating nutrients uptake rate by algal photosynthesis. The mean daily phosphorus uptake rate of phytoplankton varied between 0.04-0.11 and 0.027-0.053 g/m2/d in station I and station II respectively. The turnover time of phosphorus in phytoplankton metabolism ranged from 0.75-5.0 days during 1979-1986. The available P was 0.176 (+/- 0.156) g/m3 (mean +/- SD) in 1982 and 0.591 (+/- 0.24) g/m3 in 1986. The relationship between P/B ratio (Y) and TP (X: mg/l) was described by the following regression equation Y = 1.163 + 0.512logX (r = 0.731, P < 0.001). The dynamics of algal biomass and algal species succession were monitored as the indicators of environmental enrichment. The small-sized algae have replaced the blue-green algae as the dominant species during 1979-1986. The small-sized algae include Merismopedia glauca, Cryptomonas ovata, Cryptomonas erosa, several species Cyclotella. There has been drastic decrease in algal biomass and an obvious increase in P/B ratio. A nutrient competition hypothesis is proposed to explain the reason of the disappearance of blue-green algae bloom. The drastic change in algal size and the results in high P/B ratio (reaching a maximum mean daily ratio of 1.09 in 1986) may suggest a transition of algal species from K-selection to r-selection in Lake Donghu.

H+ISFET-based biosensor for determination of penicillin G

A biosensor based on an H+ ion sensitive field effect transistor (H+-ISFET) and penicillin G acylase has been developed. The response time of the sensor to different concentrations of penicillin G was 30 s. In a 20 mM phosphate buffer at pH 7.0, the linear range of the calibration curve was from 0.5 to 8 mM. The coefficients of variation for three samples with 20 repeated measurements were below 5%. Stability of the sensor could reach about 6 months and more than 1000 runs were performed without a significant decrease of the output value. The sensor was tested for measurement of the penicillin G content in penicillin fermentation broth. Forty samples with low and high concentrations of penicillin G were chosen for the correlation test. The values assayed by the sensor method were compared with the values assayed by HPLC method, the correlation coefficient (r) was 0.9944 and the regression equation was y = 1.034X - 2083.7 respectively. The different measuring methods are discussed in the text. (C) 1998 Published by Elsevier Science S.A. All rights reserved.

安达市碱性草地土壤颗粒的分维特征

运用土壤颗粒质量分形模型计算松嫩平原低平地安达试区植被分布区和碱斑区样点土壤颗粒的分形维数,并建立分形维数与土壤颗粒不同粒级间的回归关系,以探讨土地碱化后土壤粒径分布的分形特征及其与土壤物理性状的关系。结果表明:安达试区土壤颗粒分形维数较高,平均分别仅有48.7×10-5cm/s(Pit A)和4.30×10-6cm/s(Pit B),反映了该区土壤细颗粒含量高、土壤大孔隙数量少、土壤饱和导水率低的特征;土壤颗粒分形维数与黏粒含量呈对数正相关关系,而与粉粒和砂粒含量相关性不显著,说明在安达试区,影响土壤颗粒分形维数的主要因素是黏粒含量;羊草地土壤颗粒分形维数在土壤垂直剖面上的变异较大,说明植被生长促进了土壤质地的变异;碱斑地土壤颗粒分形维数明显大于羊草地,细颗粒含量高,饱和导水率低,说明碱斑的形成恶化了土壤物理性质;土壤颗粒分形维数可以反映安达市土壤物理性质的好坏,能作为土壤退化和生态环境恶化的评价指标。研究结果可为安达市以及松嫩平原盐碱地生态环境的修复和治理提供科学依据。

链霉菌S227抗耐药菌活性成分的研究

本论文以从四川峨嵋山森林土壤中分离筛选获得的一株产抗耐药性活性化合物的链霉菌S227为材料，对发酵液中活性物质的分离纯化及抗耐药性活性进行了研究。 建立了抗耐药性活性的定性、定量检测方法。建立的管蝶法活性定量检测的标准回归方程为：D=4.8229Ln(C)+3.6326 R=0.9972 ；纸片法活性定量检测的标准回归方程为：D=5.5Ln(C)-12.794 R=0.999。 根据建立的样品活性的检测方法，测定了发酵液的初始活性。实验证明活性物质的温度、pH稳定性好。 通过活性的定性、定量追踪方法，分别利用等体积的石油醚、乙酸乙酯、正丁醇在不同的pH梯度下萃取，确定了pH3条件下正丁醇能最大程度的萃取活性物质，说明活性物质极性很大。对正丁醇萃取相经过两次硅胶柱层析及薄层层析分离得到具有抗耐药菌活性的纯化样品S227-4。 经过核磁共振氢谱、碳谱数据分析初步确定S227-4为四聚糖，通过糖的水解实验初步确定S227-4由葡萄糖和半乳糖组成。 纸片法活性检测表明S227-4具有抗耐药菌活性。采用MIC测定法对该样品抗耐药活性进行研究。在证明该样品本身不具有抗菌活性的基础上，以临床分离的耐药性金黄色葡萄球菌为指示菌，考察了该样品与抗生素联合使用时对耐药菌抗生素MIC（最小抑菌浓度）值的影响，结果表明在不影响菌体生长的浓度条件下，该样品能明显降低多株耐药菌对多种抗生素的MIC值，不同程度地恢复所测试耐药菌对相应抗生素的敏感性。如S227-4与青霉素钠联用可以使S. aureus 12352的MIC降低8倍，而与红霉素联用可以使S. aureus 12334的MIC降低128倍。 The purification process and the activity of the anti bacterial drug resistance compounds produced by Streptomyces S227 isolated from the forest soil sample of the Mountain E’MEI in Sichuan Province were studied in this thesis. Quantitative and qualitative activity assay methods of the active compounds were established. The regression equation of the tube method was D=4.8229Ln(C)+3.6326, R=0.9972. The regression equation of the paper method was D=5.5Ln(C)-12.794, R=0.999. According to the established activity assay method, the incipient activity of the broth was evaluated. And it was proved that the stability of the active compounds was good. By quantitative and qualitative activity tracing method, petroleum ether, ethyl acetate and butanol were used to extract the active compounds at different pH. The result showed that butanol was the most effective agent for active component recovery at pH3. From the butanol extraction a purified sample, S227-4, was isolated by silica gel column chromatography and thin-layer chromatography . S227-4 was proved to be a tetra- saccharide by 1H-NMR and 13C-NMR. And its monosaccharides include glucose and galactose by hydrolysate analysis. The anti-drug resistant activity of S227-4 was tested in vitro by MICs assay using different drug resistant Staphylococcus aureus strains isolated clinically. The sample itself showed no anti-microbial activity in growth inhibitory experiment, but when it was used together with different antibiotics, it could remarkably decrease the MICs of different clinically isolated drug-resistant bacterial strains to these antibiotics. For example, when S227-4 was used with penicillin, the MIC of S. aureus 12352 decreased 8 times compared with that when penicillin was used alone. Meanwhile when it was used with erythromycin the MIC of S. aureus 12334 deceased 128 times compared with erythromycin alone.

The relation between formal potential and structure of indophenols and molecular mechnism of electrooxidation

The molecular structural parameters of indophenol and its derivatives were calculated by semi-empirical molecular orbital quantum chemical method,The relation between molecular structural parameters and formal potentials was analyzed by principal factor analysis and multiple Linear regression method. It was found that the formal potential of indophenols has a good relation with two-center electron exchange energy, E-ex (2), resonance energy of O-C bond, E-ex (C-1-O), and molecular ionization potential, I-p, among 19 moleclular structural parameters. The regression equation is E-0' = 1. 47 x 10 (-3) E-ex (two) - 5. 74 x 10 (-2) E-ex (C-1 - O) - 1. 41 x 10 (-2) I-p with RC = 0. 9999 and SD = 0. 00424. It was confirmed by the relation between structure parameters and formal potentials, and the thermodynamic stability of its intermediate products that the H (+) ionization is prior to the electron transfer step in the oxidation mechanism.