Ionic liquid mediated biomass deconstruction: from analysis challenges to fermentable sugars

Ionic liquids, ILs, have recently been studied with accelerating interest to be used for a deconstruction/fractionation, dissolution or pretreatment processing method of lignocellulosic biomass. ILs are usually utilized combined with heat. Regarding lignocellulosic recalcitrance toward fractionation and IL utilization, most of the studies concern IL utilization in the biomass fermentation process prior to the enzymatic hydrolysis step. It has been demonstrated that IL-pretreatment gives more efficient hydrolysis of the biomass polysaccharides than enzymatic hydrolysis alone. Both cellulose (especially cellulose) and lignin are very resistant towards fractionation and even dissolution methods. As an example, it can be mentioned that softwood, hardwood and grass-type plant species have different types of lignin structures leading to the fact that softwood lignin (guaiacyl lignin dominates) is the most difficult to solubilize or chemically disrupt. In addition to the known conventional biomass processing methods, several ILs have also been found to efficiently dissolve either cellulose and/or wood samples – different ILs are suitable for different purposes. An IL treatment of wood usually results in non-fibrous pulp, where lignin is not efficiently separated and wood components are selectively precipitated, as cellulose is not soluble or degradable in ionic liquids under mild conditions. Nevertheless, new ILs capable of rather good fractionation performance have recently emerged. The capability of the IL to dissolve or deconstruct wood or cellulose depends on several factors, (e.g. sample origin, the particle size of the biomass, mechanical treatments as pulverization, initial biomassto-IL ratio, water content of the biomass, possible impurities of IL, reaction conditions, temperature etc). The aim of this study was to obtain (fermentable) saccharides and other valuable chemicals from wood by a combined heat and IL-treatment. Thermal treatments alone contribute to the degradation of polysaccharides (e.g. 150 °C alone is said to cause the degradation of polysaccharides), thus temperatures below that should be used, if the research interest lies on the IL effectiveness. On the other hand, the efficiency of the IL-treatment can also be enhanced to combine other treatment methods, (e.g. microwave heating). The samples of spruce, pine and birch sawdust were treated with either 1-Ethyl-3-methylimidazolium chloride, Emim Cl, or 1-Ethyl-3-methylimidazolium acetate, Emim Ac, (or with ionized water for comparison) at various temperatures (where focus was between 80 and 120 °C). The samples were withdrawn at fixed time intervals (the main interest treatment time area lied between 0 and 100 hours). Double experiments were executed. The selected mono- and disaccharides, as well as their known degradation products, 5-hydroxymethylfurfural, 5-HMF, and furfural were analyzed with capillary electrophoresis, CE, and high-performance liquid chromatography, HPLC. Initially, even GC and GC-MS were utilized. Galactose, glucose, mannose and xylose were the main monosaccharides that were present in the wood samples exposed to ILs at elevated temperatures; in addition, furfural and 5-HMF were detected; moreover, the quantitative amount of the two latter ones were naturally increasing in line with the heating time or the IL:wood ratio.

Dimensionamento de amostras para monitoramento do ácaro da leprose Brevipalpus phoenicis (Geijskes, 1939) em citros

O controle do ácaro Brevipalpus phoenicis (Geijskes, 1939), transmissor do vírus da leprose, deve ser realizado quando sua população atinge o nível de ação, obtido pelo monitoramento de sua população, por meio de amostragens. Objetivou-se determinar o tamanho da amostra aceitável para estimar a população do ácaro, para posterior tomada de decisão. O experimento foi realizado na Fazenda Cambuhy, Matão - SP, no ano agrícola 2003-2004. Escolheu-se ao acaso um talhão da variedade Valência, com oito anos de idade, plantada no espaçamento 7x3,5m, com 2.480 plantas. Nesse talhão, foram inspecionados 1; 2; 3; 5; 10 e 100% das plantas, o que corresponde a 25; 50; 74; 124; 248 e 2.480 plantas, respectivamente, em caminhamento no sentido das linhas de plantio. Foram amostrados 3 frutos ou, na ausência destes, eram analisados ramos. de acordo com os resultados obtidos, observa-se que a porcentagem de erro na estimativa da média para a porcentagem de frutos com presença de ácaros, quando se amostra apenas 1% das plantas (25 plantas), é de 50%, ou seja, para uma infestação de 10%, a variação da porcentagem de frutos infestados estaria entre 5 e 15%, levando o produtor a subestimar ou a superestimar o nível de infestação, aumentando os gastos com pulverizações desnecessárias ou um controle ineficiente do ácaro. Para que o erro na amostragem fique dentro da situação aceitável, de 20 a 30% (em média 25%) de erro, deveriam ser amostradas 105 plantas. Na porcentagem de frutos com mais de 10 ácaros, verifica-se que, para a situação aceitável (20 a 30%), devem ser inspecionadas 540 plantas.

Proteção da relação de emprego sob o prisma da dignidade da pessoa humana

This work aims to show that the protection of the employment relation is one of the determining factors to respect the principle of the human dignity. The goal is initially to show the devaluation of work from antiquity to the early twentieth century, when the constitutions began to standardize protective devices. This way, the consecration of the social labor rights in the Constitution of 1988 represents the culmination of the historical achievements. This work demonstrates that such rights can not be reduced or suppressed by political conveniences, once these rights are included in the list of immutable clauses. It is displayed that to achieve the fundamental right to work is not well advised to encourage the creation of jobs that maculates the worker s dignity. The outsourced work is, therefore, a classic example of the advancement of precarious forms of contemporary labor. It is inferred that the presence of various forms of harassment results in a degradation of working environment, bringing about dire consequences on professional and personal life of the worker. Thus, decent work must be the appropriate benchmark for the creation of new jobs. It is also shown that the flexibilization of the propaganda rights by certain pressure groups has as main goal to reduce or eliminate rights, based on fallacious data depicting an increase of competitiveness and jobs. In addition, the flexibility implies a growth of the precarization of the work - a reality felt by many workers subjected to such a situation due to the unemployment phenomenon whose origin is not in the protectionism of the norms. It is necessary to expand and structure the constitutionally legitimate institutions to monitor and curb the precarized work, as well as all practices that go against the dignity of the worker. It is also shown the loss of power of the sindicates in the last few years as a consequence of the pulverization workes and the capital attacks through the productive restructure whose outsourcings and privatizations are notorious examples

Manutenção de tratores agrícolas e condição técnica dos operadores

A manutenção dos tratores agrícolas pode ser considerada um conjunto de procedimentos que visa a manter o equipamento nas melhores condições de funcionamento, propiciando aumento da vida útil, evitando danos prematuros, eliminando os já observados e concorrendo para maior segurança no trabalho. Essa conduta, apesar de simples, exige atenção e cuidado semelhante ou até maior quando comparado a outro veículo automotor. O presente trabalho teve por objetivo analisar a regularidade e a qualidade da manutenção dos tratores da Fazenda de Ensino, Pesquisa e Produção da UNESP - Jaboticabal, utilizados para os mais variados tipos de trabalho, como aração, gradagem, pulverização e jardinagem, e avaliar a condição técnica dos operadores responsáveis pelas máquinas. Os resultados indicam que a manutenção diária não foi eficiente, o que pode resultar em redução da vida útil das máquinas. Quanto aos operadores, notou-se baixo grau de conhecimento técnico com relação à manutenção dos tratores e falta de treinamento específico.

Sobrevivência de patógenos associados a sementes de soja armazenadas durante seis meses

Sementes contaminadas ou infectadas no campo podem ter sua sanidade alterada durante o armazenamento, pela perda da viabilidade dos patógenos. O objetivo do presente trabalho foi verificar o efeito do armazenamento na qualidade sanitária de sementes de soja. Variedades de soja BRS 133, MSoy 6101, Conquista e Liderança foram inoculadas, no campo, por pulverização com suspensão de esporos de dois isolados de Colletotrichum dematium var. truncata e Phomopsis sojae. Após a colheita foram realizados testes de sanidade, sem e com desinfestação superficial das sementes com hipoclorito de sódio a 1% por 3 min. As sementes foram armazenadas em câmara fria, por seis meses, e testes de sanidade foram novamente realizados. Os resultados do trabalho permitem concluir que o armazenamento das sementes de soja contaminadas por fungos em câmara fria, por um período de seis meses, diminuiu a incidência de Phmopsis sojae e Colletotricum dematium var. truncata.

Modos de aplicação de zinco na nutricão e na produção de massa seca de plantas de trigo

Zinc application methods can affect the nutrition and the initial development of the wheat. Thus, the aim of this work was to analyze the different ways of Zn application in the soil, on nutrition and on dry matter production. A completely randomized experimental design with four replications was used. The experiment was composed by the following Zn application treatments: control (no zinc application); soil incorporation, furrow located, seeds treatment and leaf pulverization. The experimental unit was composed of a pot filled with 7 L of soil. Morphological traits for each plant were determined on 52 days after emergence by the evaluations of plant height, internodes number, tillers number and aerial part dry matter. Still, the soil and plant chemical analysis was accomplished. The furrow located method provided larger concentrations of the micronutrient available. The zinc application methods did not influence the initial growth of the wheat plants. The zinc concentration in leaves was influenced by the different application methods, being the leaf pulverization the one that was caused the largest zinc accumulation on aerial part dry matter.

Caracterização de matérias-primas regionais e desenvolvimento de formulações de massas cerâmicas para porcelanato

Initially concentrated in some poles at the South and Southeast regions of Brazil, the ceramic tiles industry became wide during the 80 s decade, with a disconcentration industrial and regional pulverization. The competitiveness in the ceramic tiles internal and external consumers markets, it has debtor the industries to invest in sophisticated products each time more, either in design or the technology, but, mainly, in its final properties. Amongst the diverse types of ceramic coating, the porcelanato if has detached had to its process of technological production and excellent characteristics techniques. The Porcelanato is currently the material for coatings that presents the best technical and aesthetic features when compared with others ceramics found on the market. The chemical composition and the others raw materials characteristics have an importance that must to be ally to the inherent characteristics of fabrication process, essentially those related to the cycle of burning. This work had as purpose to develop formularizations of ceramic mass for production of porcelanato without glass coating, pertaining to the group BIa (text of absorption of water ≤ 0.5%) and with resistance superior mechanics 35MPa from raw materials characterized. The ceramic raw materials selected to the development of this study (A1 and A2 clays, feldspate, talc and quartz) were submitted to the following tests: X-ray fluorescence - chemical analysis determination; X-ray diffraction - Analysis of the stages mineralogics; Laser granulometry - size distribution of particles; and Differential thermal analysis - thermal behavior. Were performed tests of absorption of water, lineal retraction of it burns, apparent specific mass and rupture tension the flexing. The results had evidenced that the formularizations that had the A1 clay and talc on its composition were efficient for the porcelanato production remaining their technological characteristics inside of the intervals of variation desired by the Norms of the ABNT

Ensino da astronomia no Brasil: educação formal, informal, não formal e divulgação científica

Este artigo deriva de uma pesquisa mais ampla sobre a educação em astronomia e a formação de professores, e apresenta um panorama geral sobre o tema em âmbito nacional. Procuramos gerar uma classificação das instituições e outras iniciativas brasileiras dedicadas à astronomia, levando em conta os seus objetivos, tais como o ensino formal, informal, não-formal, bem como aqueles destinados à popularização dessa ciência. Comenta-se, em forma de um breve ensaio, a importância da atuação contextualizada destas instâncias no ensino da astronomia, levantando um desafio ainda a ser considerado, referente ao estudo das possíveis relações entre estes estabelecimentos e iniciativas, visando o avanço da educação em astronomia, em um movimento contrário à dispersão e pulverização de atividades locais e pontuais dos mesmos. Argumentamos que a pesquisa em ensino de astronomia tem potencial para exercer este papel integrador.

Gibberellic acid and water regime in the flowering induction of Brassocattleya and Cattleya hybrid orchids

No presente trabalho foi avaliada a influência do ácido giberélico e do regime hídrico na indução e qualidade do florescimento de duas orquídeas híbridas dos gêneros Cattleya (C.) e Brassocattleya (Bc.). O experimento foi realizado no Setor de Biotecnologia e Orquidicultura da Fundação Shunji Nishimura de Tecnologia, Pompéia-SP. Foram testadas cinco concentrações de GA3 (0, 125, 250, 500 e 1.000 mg L-1) em quatro aplicações consecutivas via pulverização foliar, em plantas adultas que já haviam florescido ao menos uma vez, além de duas condições hídricas (uma e quatro irrigações por semana). As aplicações foram feitas nos meses de outubro e novembro para Bc. Marcella Koss e janeiro e fevereiro para C. Irene Holguin. Não foi possível induzir a floração em Cattleya Irene Holguin com o uso de ácido giberélico. Para Bc. Marcella Koss, a aplicação de 250 mg L-1 de GA3, associado à diminuição na frequência de irrigação, induziu cerca de 83% das plantas ao florescimento. Na mesma concentração de GA3, porém em condições de irrigação frequente, apenas 17% das plantas foram induzidas a florescer. O número e o tamanho das flores aumentaram com a aplicação de concentrações maiores de GA3 utilizadas no experimento. A realização deste trabalho permitiu desenvolver uma técnica comercial com o uso de ácido giberélico (GA3) para a indução do florescimento do híbrido de orquídea Bc. Marcella Koss.

Diversidade de ácaros (Acari, Arachnida) em seringueiras (Hevea brasiliensis Muell. Arg., Euphorbiaceae) na região noroeste do Estado de São Paulo, Brasil

The mites of three rubber tree cultures (Cedral, Pindorama and Taquaritinga) in order to determine the abundance of populations, the richness, the diversity and the degree of similarity among the communities was studied. Twenty one species were found, five of which were common to the three cultures. The richness and the abundance were greatest at the beginning of the dry season. The composition of communities differed probably as consequence of the kind of neighboring vegetation to each area, and because of the acaricid pulverization on the culture of Taquaritinga, reductng the richness of mite species in that area. The influence of neighboring vegetation can be shown by the occurrence of Iphiseiodes zuluagui Denmark & Muma, 1972, a common species to citrus trees, on neighboring rubber trees in Taquaritinga, and Tenuipalpus heveae Baker, 1945, a common species on rubber trees, on a coffee culture neighbor to the rubber trees of Pindorama. This data suggests that mites move among neighbor cultures, and can be an importam factor towards pest management and control. The diversity was small on the three cultures, as a result of the occurrence of one dominam species on each area, Calacarus heveae Feres, 1992 or T. heveae, which are considered pests of the rubber tree. The small diversity and the occurrence of dominant species are patterns expected in monocultures, systems with small environmental heterogeneity.

Seeder-fertilizer machine: energetic demand as a function of vegetal covering handling and manure deposition shank depth in no-tillage system

O trabalho teve por objetivo avaliar a demanda energética de uma semeadora-adubadora, em função do tipo e manejo da cultura de cobertura vegetal e da profundidade da haste de deposição de adubo. Foi utilizado um trator Valtra BM100, instrumentado, para tracionar uma semeadora-adubadora de precisão equipada com quatro fileiras de semeadura espaçadas de 0,9 m para cultura de milho. O experimento foi conduzido em parcelas subsubdivididas, na área experimental do Laboratório de Máquinas e Mecanização Agrícola (LAMMA) da UNESP-Jaboticabal, utilizando duas culturas de cobertura (mucuna-preta e crotalária), três manejos dessas coberturas, sendo dois mecânicos (triturador de palhas e rolo-faca) e um químico (pulverização com herbicida), realizados 120 dias após a semeadura das culturas de cobertura e três profundidades da haste de deposição do adubo (0,11; 0,14 e 0,17 m), perfazendo 18 tratamentos, com quatro repetições, totalizando 72 observações. Foram avaliados os parâmetros velocidade de deslocamento, patinagem, força na barra de tração, força de pico, potência na barra de tração, potência de pico e consumo de combustível. Pôde-se concluir que a força na barra de tração foi menor para as profundidades de 0,11 e 0,14 m da haste sulcadora de adubo, o mesmo ocorrendo para força de pico, potência na barra de tração e consumo volumétrico. O consumo específico foi menor na profundidade de 0,17 m da haste sulcadora de adubo. As culturas de cobertura e seus manejos não interferiram no desempenho das máquinas estudadas.

Efeito do Tratamento com Hidróxido de Sódio sobre a Fração Fibrosa, Digestibilidade e Tanino do Feno de Jurema-Preta (Mimosa tenuiflora. Wild)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of different methods of cutting fluid application on turning of a difficult-to-machine steel (SAE EV-8)

In this study, different methods of cutting fluid application are used in turning of a difficult-to-machine steel (SAE EV-8). Initially, a semisynthetic cutting fluid was applied using a conventional method (i.e. overhead flood cooling), minimum quantity of cutting fluid, and pulverization. A lubricant of vegetable oil (minimum quantity of lubricant) was also applied using the minimum quantity method. Thereafter, a cutting fluid jet under high pressure (3.0 MPa) was singly applied in the following regions: chip-tool interface, top surface of the chip (between workpiece and chip) and tool-workpiece contact. Moreover, two other methods were used: an interflow between conventional application and chip-tool interface jet (combined method) and, finally, three jets simultaneously applied. In order to carry out these tests, it was necessary to set up a high-pressure system using a piston pump for generating a cutting fluid jet, a venturi for fluid application (minimum quantity of cutting fluid and minimum quantity of lubricant) and a nozzle for cutting fluid pulverization. The output variables analyzed included tool life, surface roughness, cutting tool temperature, cutting force, chip form, chip compression rate and machined specimen microstructure. Among the results, it can be observed that the tool life increases and the cutting force decreases with the application of cutting fluid jet, mainly when it is directed to the chip-tool interface. Excluding the methods involving jet fluid, the conventional method seems to be more efficient than other methods of low pressure, such as minimum quantity of volume and pulverization, when considering just the cutting tool wear. © 2013 IMechE.

Different methods of cutting fluid application on turning of a difficult-to-machine steel

Different methods of cutting fluid application are used on turning of a difficult-tomachine steel (SAE EV-8). A semi-synthetic cutting fluid was applied using a conventional method, minimum quantity of cutting fluid (MQCF), and pulverization. By the minimum quantity method was also applied a lubricant of vegetable oil (MQL). Thereafter, a cutting fluid jet under high pressure (3.0 MPa) was singly applied in the following regions: chip-tool interface; top surface of the chip; and tool-workpiece contact. Two other methods were used: an interflow between conventional application and chip-tool interface jet and, finally, three jets simultaneously applied. In order to carry out these tests, it was necessary to set up a high pressure system using a piston pump for generating a cutting fluid jet, a Venturi for fluid application (MQCF and MQL), and a nozzle for cutting fluid pulverization. The output variables analyzed included tool life, surface roughness, cutting tool temperature, cutting force, chip form, chip compression rate and machined specimen microstructure. It can be observed that the tool life increases and the cutting force decreases with the application of cutting fluid jet, mainly when it is directed to the chip-tool interface. Excluding the methods involving jet fluid, the conventional method seems to be more efficient than other methods of low pressure. © (2013) Trans Tech Publications, Switzerland.

Volume de aplicação na qualidade da deposição da pulverização na cutura do algodoeiro

Pós-graduação em Agronomia (Agricultura) - FCA