Ionically bound peroxidase from peach fruit

Soluble, ionically bound peroxidase (POD) and polyphenoloxidase (PPO) were extracted from the pulp of peach fruit during ripening at 20degreesC Ionically bound form was purified 6.1 -fold by DEAE-cellulose and Sephadex G-100 chromatography. The purified enzyme showed only one peak of activity on Sephadex G-100 and PAGE revealed that the enzyme was purified by the procedures adopted. The purified enzyme showed a molecular weight of 29000 Da, maximum activity at pH 5.0 and at 40degreesC the calculated apparent activation energy (Ea) for the reaction was 10.04 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 75degreesC with a fast inactivation at 75degreesC Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar concentrations (0, 10, 20 %, w/w), with an activation energy (Ea) for inactivation increasing with sucrose concentration from 0 to 20% (w/w). The Km and V-max values were 9.35 and 15.38 mM for O-dianisidine and H2O2, respectively. The bound enzyme was inhibited competitively by (.)ferulic, caffeic and protocatechuic acids with different values of Ki,. L-cysteine, p-coumaric and indolacetic acid and Fe++ also inhibited the enzyme but at a lower grade. N-ethylmaleimide and p-CMB were not effective to inhibit the enzyme demonstrating the non-essentiality of SH groups.

Avaliações sensoriais, bioquímicas e microbiológicas do guacamole, um produto à base de abacate, sob armazenamento a frio e com adição de ácido ascórbico

The elaboration of avocado products for commercialization keeping their characteristics of fresh product has been limited. The cut avocado darkens quickly and their sensorial characteristics are modified with the storage. In the present research, the sensorial parameters, microbiological stability, and peroxidase and polyphenoloxidase activity were evaluated in guacamole added with ascorbic acid and conserved under low temperature, by using avocado variety Hass. Products were conditioned in polyethylene+nylon packages with and without vacuum application; then, they were subjected to the slow and fast freezing (-18 degrees C) and stored in freezer (-18 degrees C). Evaluations were performed at the moment of elaboration of the product (t0) and at 3, 7 and 30 days post-storage. At t30, samples were kept under refrigeration (4 +/- 1 degrees C) and evaluated at 3, 5 and 7 days. After the 30 days of storage, -18 degrees C under freezing, followed by thawing and keeping at 4 +/- 1 degrees C for 7 days, the notes for the sensorial parameters decreased. The peroxidase activity was totally inhibited in the elaborated product and the polifenol oxidase activity considerably decreased in the guacamole (20.07 mM catechol/g fresh matter) relative to those in the fruit (58.31 mM catechol/g fresh matter), however with no significant variation during storage (at -18 degrees C). The samples were microbiologically stable under the conditions of the present study. The addition of ascorbic acid contributed to the conservation of the frozen avocado product by decreasing the enzymatic activity. However, the sensorial parameters are prejudiced under thawing and storage at 4 +/- 1 degrees C.

Mandioca minimamente processada submetida a radiação gama

Fresh cut cassava is an alternative to prolong roots commercialization period, adding value to the raw material and assisting the consumer's needs that seeks for products of easy preparation. The aim of this work was to evaluate the quality of fresh cut cassava subjected to gamma radiation. For processing cassava roots (IAC - 567-70) were used, after preparation were packed in expanded polystyrene (EPS) trays, covered by low density polyethylene (LDPE) plastic film, being subjected to gamma radiation in the doses 0 (control), 0.5; 1.0; 1.5; 2.0 KGy. Another treatment was also carried out in which roots were vacuum packed in nylon + polyethylene. Packed roots of all treatments were stored under refrigeration (5 +/- 1 degrees C) and evaluations were made after 0, 3, 6, 9 and 12 days. Evaluated analyses were: titrable acidity, pH, firmness, coloration, polyphenoloxidase activity, cooking time and roots hydration percentage. Evaluations just proceeded until the nine storage day, because after this period the roots already presented visually detectable physiologic and microbiological alterations. For titrable acidity, the vacuum packed roots had the lowest values, followed by the control packed in trays. For the other treatments, values of acidity increased and influenced the pH values. For the other appraised parameters there was no significant difference among treatments, but alterations were observed with the storage. The firmness decreased with the storage. Brightness values and yellow color component decreased with the storage and the b * (green) color values increased. Polyphenoloxidase activity was maximum in the day of elaboration of the product and it decreased with the storage. Cooking time and roots hydration percentage decreased with the roots storage.

Evaluation of pyraclostrobin and acibenzolar-S-methyl on common bacterial blight of snap bean

Assays were done under greenhouse conditions in order to evaluate the effect of pyraclostrobin (0.0375, 0.0750 and 0.150 mL.L-1) and acibenzolar-S-methyl (ASM) (0.025 g.L-1) in common bacterial blight on leaves of snap beans cultivar Braganca. These chemicals were sprayed at three different times: five days before; five days before + five days after; and five days after leaf inoculation with an isolate of Xanthomonas axonopodis pv. phaseoli. They were determinate the levels of polyphenoloxidase, peroxidase and total soluble proteins on inoculated and non-inoculated leaves of snap beans sprayed with pyraclostrobin (0.075 g.L-1) and ASM (0.025 g.L-1). All concentration of pyraclostrobin and ASM reduced the area under the disease progress curve (AUDPC) on leaves of snap beans, and the least AUDPC value was observed when this products were sprayed five days before + five days after inoculation. Higher levels of polyphenoloxidase, peroxidase and the total soluble proteins were observed on leaves sprayed with pyraclostrobin or ASM.

Atividades de peroxidase e polifenoloxidase durante o armazenamento pós - Colheita de yacon (polymnia sonchifolia poep & endl)

The purpose of this study was to investigate the peroxidase and polyphenoloxidase activities during the refrigeration at 4oC of yacon tuberous roots with and without polyethylene packing and compared with untreated roots at regular conditions. It was concluded that refrigeration treatment maintains a low activity of these enzymes and keeps good appearance of the roots.

Use of modified atmosphere to extend shelf life of fresh-cut carambola (Averrhoa carambola L. cv. Fwang Tung)

Fresh-cut fruit products, including carambola (Averrhoa carambola L.) have limited marketability due to cut surface browning attributed to oxidation of phenolic compounds by enzymes such as polyphenol oxidase (PPO). The objective of this study was to evaluate postharvest changes of carambola slices in three different packagings. Carambola fruit (cv. Fwang Tung) were picked from the orchard of Estação Experimental de Citricultura de Bebedouro at mature-green stage. Fruit were washed, dipped in NaOCl solution (200 mg.L -1 for 5 minutes), and stored overnight at 10°C. Fruit were manually sliced into pieces of approximately 1 cm. Slices were rinsed with NaOCl solution at 20 mg.L-1, drained for 3 minutes, and packaged in polyethylene terephthalate (PET) trays (Neoform N94); polystyrene trays covered with PVC 0.017 mm (Vitafilm - Goodyear); and vacuum sealed polyolefin bags (PLO, Cryovac PD900). The packages were stored at 6.8°C and 90%RH for 12 days and samples taken every 4 days. PET trays and PVC film did not significantly modify internal atmosphere and the high water permeability of PVC led to more rapid slice desiccation. PPO activity was lower when slices were packaged in PLO vacuum sealed bags, which reduced discolouration and led to better appearance maintenance for up to 12 days.

Efeito de produtos químicos sobre a mancha bacteriana (xanthomonas perforans) e na ativação de proteínas relacionadas à patogênese em tomateiro

The present study aims to evaluate the effect of fungicides and antibiotics to control bacterial spot (Xanthomonas perforans) in tomato, and the activation of pathogenesis-related proteins. Hybrid tomato AP 529 was used to assess the severity of disease. The treatments consisted of spraying with acibenzolar-S-methyl, fluazinam, pyraclostrobin, pyraclostrobin + methiran, copper oxychloride, copper oxychloride and mancozeb + oxytetracycline, and inoculated and non-inoculated controls. After three days of treatment, all plants were inoculated with X. perforans (10 6 CFU / mL). Leaf discs were collected for assessment of peroxidase, polyphenol oxidase, β-1,3 glucanase, phenylalanine ammonia lyase and protease. The area under the disease progress curve (AUDPC) was calculated with the data of severity. All treatments had reduced AUDPC compared to the inoculated control. Fungicides acibenzolar-S-methyl, pyraclostrobin, and pyraclostrobin + methiran had more satisfactory results in reducing the severity of bacterial spot on tomato. The products based on pyraclostrobin together with acibenzolar-S-methyl induced enzymatic activities of peroxidase, polyphenoloxidase and β-1,3 glucanase, indicating that these products may be related to the induction of resistance to bacterial spot on tomato plants.

Estádios de maturação de pêssegos 'Aurora-1' para o processamento mínimo

The experiment aimed to verify the effect of maturation stage on the quality of minimally processed peaches. Fruits were used in two stages of maturity, on time corresponding to the background color yellow-green, and mature, which corresponds completely to the background color yellow. The minimum process consisted of washing, sanitizing, enzyme peel, cut lengthwise and removed the stone fruits. Halves obtained were immersed in water chlorinated to 10 mg L-1 of water and left on standing to drain the excess liquid. Afterwards, it was proceeded the packaging of the halves in containers of polyethylene terephthalate (PET) and with transparent lid, and the storage at 3 ± 2 ° C and RH = 65% for 12 days, with assessments every three days. The variables evaluated were appearance, weight loss, firmness, soluble solids, titratable acidity, soluble and reduced sugars, ascorbic acid, total and soluble pectin, coloring and activity of polyphenoloxidase. The storage of minimally processed peaches 'Aurora-1' mature harvested was limited mainly by the loss of freshness and firmness, and because they have darker appearance, and lower levels of reduced sugars and ascorbic acid. Peaches 'Aurora-1', harvested at the maturity stage on time, had better quality and longer duration of their minimally processed products.

Qualidade de mangas cv. palmer após armazenamento sob baixas temperaturas

This study aimed to evaluate the quality of 'Palmer' mangoes previously stored at low temperatures, after their transference to the environmental condition. Fruits harvested at physiological maturity were carefully transported to the Laboratory where they were selected, standardized as the color, size and absence of injuries and treated with fungicide before they were stored at 2°C (75.7% RH), 5°C (73.8% RH) e 12°C (82% RH) for 7, 14 and 21 days. At the end of each period, the fruits were transferred to environmental condition (22.9°C; 62.3% RH), where they were kept for 1, 3, 5 and 7 days, simulating the trading period, and evaluated for the occurrence of injuries and rottenness; peel and pulp color; firmness; contents of soluble solids, titratable acidity and ascorbic acid, as well as, the activities of the enzymes peroxidase, polyphenoloxidase and phenylalanine ammonia-lyase. The results indicated that 'Palmer' mangoes can be stored at 12°C for 21 days without damage to ripening, but with limitations due to the occurrence of decay. The storage at 2°C and 5°C was limited by the occurrence of injuries in the peel, but at the temperature of 2°C these symptoms were more severe and compromised the development of the characteristic color of the peel. However, the ripening of the pulp was not harmed, but this process occurred with less intensity than in mangoes maintained at 12°C.

Storage of sugarcane stalks (Saccharum officinarum cv. SP 79-1011) in low oxygen atmospheres and the effects on enzymatic browning

Although sugarcane consumption is very popular worldwide there are few studies regarding the postharvest storage of stalks that focus on controlling enzymatic browning. The objective of this study was to evaluate the quality of sugarcane stalks stored at 10±1°C in controlled atmosphere with various oxygen (O2) levels (1kPa, 5kPa, 10kPa, 15kPa, and 21kPa) and to verify the effect of this storage on the activities of polyphenoloxidase (PPO) and peroxidase (POD). At 1kPa and 5kPa O2, the stalks' respiratory rate, POD activity, and reducing sugar content were significantly less (P<0.05) than at higher O2 concentrations. In contrast, low O2 levels did not affect PPO activity or influence cane coloration. These results can be used to guide the choice of plastic films, making it possible to store sugarcane stalks in a controlled atmosphere. © 2013 Elsevier B.V.

Contribuição ao estudo bioquímico de polpa de híbridos da cultivar maracujá amarelo azedo (Passiflora edulis f. flavicarpa Degener): enzimas e compostos bioativos

Pós-graduação em Biotecnologia - IQ

Avaliação da capacidade antioxidante de frações orgânicas do cogumelo Agaricus blazei Murril e purificação parcial de peroxidase e polifenoloxidase

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de ciclodextrina glicosiltransferase (CGTase) e de ciclodextrinas como coadjuvante na inibição do escurecimento em alimentos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Escurecimento enzimático em frutos: polifenoloxidase de atemóia (Annona cherimola Mill. X Annona squamosa L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Aplicação de 1-mcp na conservação de abacate ‘Hass’

Pós-graduação em Agronomia (Energia na Agricultura) - FCA