Glomus intraradices and Gigaspora margarita arbuscular mycorrhizal associations differentially affect nitrogen and potassium nutrition of Plantago lanceolata in a low fertility dune soil

Two controlled microcosm experiments aimed at a critical re-assessment of the contributions of divergent arbuscular mycorrhizal (AM) fungi to plant mineral nutrition were established that specifically targeted Plantago lanceolata–Glomus intraradices (B.B/E) and –Gigaspora margarita (BEG 34) symbioses developed in a native, nutrient limited, coastal dune soil. Plant tissue nitrogen (N), phosphorus (P) and potassium (K) status as well as plant growth parameters and levels of mycorrhizal colonization were assessed at harvest. In addition to the general well-established mycorrhizal facilitation of P uptake, the study was able to demonstrate a G. intraradices-specific contribution to improved plant nitrogen and potassium nutrition. In the two respective experiments, G. intraradices-inoculated plants had 27.8% and 40.8% more total N and 55.8% and 23.3% more total K when compared to Gi. margarita inoculated counterparts. Dissimilar overall contribution of the two isolates to plant nutrition was identified in AM-genus specific differences in plant tissue N:P:K ratios. G. intraradices inoculated and non-mycorrhizal plants generally exhibited N:P:K ratios indicative of P limitation whereas for Gi.margarita mycorrhizal plants, corresponding ratios strongly implied either N or K limitation. The study provides further evidence highlighting AM functional biodiversity in respect to plant nutrient limitation experienced by mycorrhizal P. lanceolata in an ecologically relevant soil system.

Spider-fed bromeliads: seasonal and interspecific variation in plant performance

Background and Aims Several animals that live on bromeliads can contribute to plant nutrition through nitrogen provisioning (digestive mutualism). The bromeliad-living spider Psecas chapoda (Salticidae) inhabits and breeds on Bromelia balansae in regions of South America, but in specific regions can also appear on Ananas comosus (pineapple) plantations and Aechmea distichantha. Methods Using isotopic and physiological methods in greenhouse experiments, the role of labelled ((15)N) spider faeces and Drosophila melanogaster flies in the nutrition and growth of each host plant was evaluated, as well as seasonal variation in the importance of this digestive mutualism. Key Results Spiders contributed 0.6 +/- 0.2% (mean +/- s.e.; dry season) to 2.7 +/- 1% (wet season) to the total nitrogen in B. balansae, 2.4 +/- 0.4% (dry) to 4.1 +/- 0.3% (wet) in An. comosus and 3.8 +/- 0.4% (dry) to 5 +/- 1% (wet) in Ae. distichantha. In contrast, flies did not contribute to the nutrition of these bromeliads. Chlorophylls and carotenoid concentrations did not differ among treatments. Plants that received faeces had higher soluble protein concentrations and leaf growth (RGR) only during the wet season. Conclusions These results indicate that the mutualism between spiders and bromeliads is seasonally restricted, generating a conditional outcome. There was interspecific variation in nutrient uptake, probably related to each species` performance and photosynthetic pathways. Whereas B. balansae seems to use nitrogen for growth, Ae. distichantha apparently stores nitrogen for stressful nutritional conditions. Bromeliads absorbed more nitrogen coming from spider faeces than from flies, reinforcing the beneficial role played by predators in these digestive mutualisms.

PHOSPHORUS EFFECTS IN THE NUTRITION and GROWTH of DEVELOPING MANGO PLANTS

The objective was to study the effects of phosphorous (P) fertilization on nutritional and developmental aspects of growing mango plants. The mango plants were evaluated by soil chemical analyses, leaf chemical analyses, biological examination of plant growth, and the starting point of fruit production. Having this in view, an experiment was set up on 2 January 2003, at Flora, a farm in Uberlandia, state of Minas Gerais, Brazil. The soil was a clayish Oxisol. The doses at planting were: D0 = zero, D1 = 40, D2 = 100, D3 = 200, and D4 = 300 g of P2O5 plant-1. These doses at the beginning of the second year were multiplied by 1.5 and at the beginning of the third year by 2.0 and applied to the plants. The fertilizer used in this experiment was triple superphosphate (44% of P2O5.). During August of 2004, 2005, and 2006, soil samples were taken at a depth of 20 cm in between the plant rows. Leaf samples were taken during August of 2004 and 2005 to determine macro- and micronutrient contents in the leaves. Plant stem diameter was measured during January of 2004 and 2005. Plant height and crown radius were measured during January of 2005 and fruit production in 2005 and 2006. Fertilizer applications increased the level of P in the soil but significantly influenced plant performance only after the second year. The effects of phosphorus on mango plants take place slowly leading to increments in plant stem diameter only at the third year. Fruit set was not influenced by phosphorous fertilization.

Influence of potassium nutrition and the nitrate ammonium ratio on the putrescine and spermidine contents in banana vitroplants

Potassium (K) is required in high doses by the banana (Musa sp.) plant and interacts with other nutrient elements in which banana tissues are maintained under in vitro condition as a consequence modifications in the plant metabolism take place mainly in nitrogen (N) compounds, such as proteins, amino acids, and secondary compounds. When K is present in concentrations lower than that required, diamines such as putrescine and poliamines are formed. This metabolic disorder can also be correlated with the presence of different inorganic N forms, such as nitrate (NO3) and ammonium (NH4), and the ratios between both ions as well. In order to follow the physiological performance of the interrelationships, K/putrescine and of the NO3/NH4 ratio in the tissue of banana vitroplantlets, shoot apex of two banana cvs. Nanica and Prata Ana were maintained in modified MS medium in the presence of six different doses of K: 5, 10, 15, 20, 25, and 30 mM. After the period of tissue proliferation the cultures were transferred to rooting media containing the same different K doses. Dry matter, K, putrescine, and spermidine contents and their accumulation were determined in the shoots and roots of the vitroplantlets and in the shoot apex of the explant donor cultivar as well as the corresponding values for the whole vitroplantlets calculated. The data were statistically analyzed. The contents and accumulations of putrescine and spermidine in banana tissues were enhanced as K concentration decreased in the medium: four times (0.19% of the dry matter) for cv. Nanica and eight times (0.25% of the dry matter) for cv. Prata Ana. This behavior was not only related to the K depletion but to the NO3/NH4 ratio as well.

Early development and nutrition of cover crop species as affected by soil compaction

A good cover crop should have a vigorous early development and a high potential for nutrient uptake that can be made available to the next crop. In tropical areas with relatively dry winters drought tolerance is also very important. An experiment was conducted to evaluate the early development and nutrition of six species used as cover crops as affected by sub-superficial compaction of the soil. The plants (oats, pigeon pea, pearl millet, black mucuna, grain sorghum, and blue lupin) were grown in pots filled with soil subjected to different subsurface compaction levels (bulk densities of 1.12, 1.16, and 1.60 mg m(-3)) for 39 days. The pots had an internal diameter of 10 cm and were 33.5 cm deep. Grasses were more sensitive to soil compaction than leguminous plants during the initial development. Irrespective of compaction rates, pearl millet and grain sorghum were more efficient in recycling nutrients. These two species proved to be more appropriate as cover crops in tropical regions with dry winters, especially if planted shortly before spring.

Kinetics of zinc uptake and anatomy of roots and leaves of coffee trees as affected by zinc nutrition

Zinc (Zn) uptake kinetics and root and leaf anatomy were studied in coffee trees grown in nutrient solutions with or without Zn. Leaves and roots were sampled and cuts were made in the medium part of the leaves and in root tips and observed under an optical microscope. Plants grown without Zn showed an increase in root and in root stele diameter. There was also an increase in epidermis thickness and in the cross-sectional area of the cortex and stele due to Zn deficiency, but the diameter of xylem vessels was decreased. An increase in root cortex and stele diameter provided for an increased surface for nutrient uptake. Accordingly, C(min) was decreased from 13.8 to 3.4 mu mol L(-1) and V(max) increased from 0.50 to 2.1 mu mol cm(-2) h(-1) .

Cotton growth and boron distribution in the plant as affected by a temporary deficiency of boron

The knowledge of nutrient mobility is an important tool to define the best fertilizer management and diagnosis techniques. Patterns of boron (B) mobility in plants have been reviewed, but there is very little information on B distribution and mobility in cotton. An experiment was conducted to study plant growth and B distribution in cotton when the nutrient was applied in the nutrient solution or to the leaves, and when a temporary deficiency was imposed. Cotton (Gossypium hirsutum, Latifolia, cv. IAC 22) was grown in nutrient solutions where B was omitted or not for 15 days. Boron was applied to young or mature cotton leaves in some of the minus B treatments. Root growth decreased when the plants were transferred to B solutions, but there was a full recovery when B was replaced in the nutrient medium. Boron deficiency, even when temporary, reduced cotton shoot dry matter yields, plant height and flower and fruit set, and these could not be prevented by foliar application of B. Because of decreased dry matter production, leaves of deficient cotton plants actually showed higher B concentrations than non deficient leaves. This would be misleading when a mature leaf is sampled for diagnosis. If there is any B mobility in cotton phloem, it is very low.

The plant ionome revisited by the nutrient balance concept

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Applying boron to coconut palm plants: effects on the soil, on the plant nutritional status and on productivity boron to coconut palm trees

Boron, one of the micronutrients frequently found in low levels in tropical soils affects nutrition and productivity of coconut palm trees essentially cultivated in tropical climates. The objective of this research study was to evaluate the effect of boron on the nutritional status of the plant and its productivity when artificially applied to the culture soil. The experiment was carried out in a four year old, artificially irrigated, dwarf coconut palm orchard in Brazil, between January, 2005 and October, 2006. The soil was a red yellow Latosol (B: 0.18 mg dm(-3)). The treatments consisted in the application of five boron dosages: zero, 1, 2, 4, and 6 kg ha(-1). In the field, the treatments were arranged according to a completely randomized block design, with four replications. Boron (borax) dosages were applied in equal halves directly into the soil in the months of January and February of 2005. Boron concentration in the soil and plant and plant productivity were evaluated. The higher palm tree production was associated to levels of 0.6mg dm(-3) of B in the soil and 23.5mg kg(-1) in leaves. Ninety five percent of palm trees maximum production was obtained with the use of a boron dosage of 2,1kg ha(-1).

Potassium nutrition in the first and second ratoon sugarcane grown in an Oxisol by a conservationist system

The presence of mulch on the soil surface after the mechanical harvesting of sugarcane (Saccharum officinarum L.) enhances the cycling of nutrients, especially K, which can decrease K fertilizer recommendations for the crop. The aim of this study was to evaluate the effect of K addition to an Oxisol, with an initial concentration 0.07 cmol(c) K kg(-1), in first and second ratoon (no-till) sugarcane cultures by a conservationist system, i.e. rational use of fertilizers, use of alternative inputs and especially the maintenance of residues in soil that was previously burned to facilitate cutting. The following K doses were tested: 0, 32.5, 65, 130, and 195 kg K2O ha(-1), arranged in a randomized block design with five replicates. Potassium content in the soil and in the plant, as well as the yield and the quality of stalks were evaluated. Soil K application increased K concentration in soil and plant, and was reflected in the prodUction of stalks, with higher production (87.5 and 107.5 t ha(-1)) with the use of 120 and 123 kg K2O ha(-1) in first and second ratoon sugarcane, respectively. At the first 2 yr it was not possible to reduce the K fertilization in ratoon. Therefore, with the introduction of the conservationist system there was an increase (20 t ha(-1)) at the second ratoon regarding the first one with the same applied rate.

Nitrogen budget in a soil-plant system after brachiaria grass desiccation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Plant iononne diagnosis using sound balances: case study with mango (Mangifera Indica)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Soil fertility, nutrition and early growth of physic nut as affected by nitrogen fertilization

The initial growth and mineral nutrition of the physic nut (Jatropha curcas L.) as a function of nitrogen (N) fertilization was investigated. The transplanting of seedlings was carried out in plastic pots filled with 50 dm3 of a Rhodic Hapludox, under a plastic greenhouse. In addition to a control treatment, the dosages of 0, 40, 80, 120, and 160 mg dm−3 N were tested. The results demonstrated that N fertilization for the cultivation of physic nut could be added as top dressing from 60 days after planting with a dosage of 65 mg dm−3. A SPAD index of 46 can be used as a nutritional reference to its initial development. Furthermore, the results suggested that the order of nutrient accumulation by the physic nut plants is as follows: potassium (K) > N > magnesium (Mg) > calcium (Ca) > phosphorus (P) > sulfur (S) > iron (Fe) > manganese (Mn) > boron (B) > zinc (Zn) > copper (Cu).

Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency

The objective of this work was to study the effect of root and foliar application of two commercial products containing amino acids from plant and animal origin on iron (Fe) nutrition of tomato seedlings cultivated in two nutrient media: lime and normal nutrient solutions. In the foliar-application experiment, each product was sprayed with 0.5 and 0.7 mL L–1 2, 7, 12, and 17 d after transplanting. In the root application experiment, 0.1 and 0.2 mL L–1 of amino acids products were added to the nutrient solutions. In both experiments, untreated control plants were included as well. Foliar and root application of the product containing amino acids from animal origin caused severe plant-growth depression and nonpositive effects on Fe nutrition were found. In contrast, the application of the product from plant origin stimulated plant growth. Furthermore, significantly enhanced root and leaf FeIII-chelate reductase activity, chlorophyll concentration, leaf Fe concentration, and FeII : Fe ratio were found in tomato seedlings treated with the product from plant origin, especially when the amino acids were directly applied to the roots. These effects were more evident in plants developed under lime-induced Fe deficiency. The positive results on Fe uptake may be related to the action of glutamic acid, the most abundant amino acid in the formulation of the product from plant origin.