Heliconia acuminata reproductive success is independent of local floral density

Reproductive plants in tropical forests are patchily distributed, with some in large aggregations of reproductive consepecifics while others are relatively isolated. This variation in floral density is hypothesized to have a major effect on plant reproductive success, since individuals in higher density neighborhoods can attract more or higher quality pollinators. We experimentally tested this hypothesis with populations of the understory herb Heliconia acuminata in central Amazonia. We created replicated plots in which reproductive plant density spanned the range of naturally occurring floral neighborhood size, then measured three surrogates of plant fitness in focal plants in each array. There was no significant difference between any of the three floral neighborhood treatments in total seed production, fruit set, or the number of seeds produced per fruit. Pollinator visitation rates to plants in all treatments were extremely low, with many plants not visited at all during the observation period. This could be because H. acuminata's hummingbird pollinators are unable to find the widely scattered reproductive plants, however this hypothesis appears unlikely. Instead, natural flowering plant densities may simply be below the threshold value at which neighborhood effects become important, even in "high-density" aggregations. Nutrient limitation, selective fruit abortion, and reproduction via male rather than female function may also be playing a role. We argue the absence of neighborhood effects may be a general phenomenon in central Amazonian forests, though additional experiments with other plant-pollinator systems are needed to determine the extent to which this hypothesis is supported.

Two's company, three's a crowd: experimental evaluation of the evolutionary maintenance of trioecy in Mercurialis annua (Euphorbiaceae).

Trioecy is an uncommon sexual system in which males, females, and hermaphrodites co-occur as three clearly different gender classes. The evolutionary stability of trioecy is unclear, but would depend on factors such as hermaphroditic sex allocation and rates of outcrossing vs. selfing. Here, trioecious populations of Mercurialis annua are described for the first time. We examined the frequencies of females, males and hermaphrodites across ten natural populations and evaluated the association between the frequency of females and plant densities. Previous studies have shown that selfing rates in this species are density-dependent and are reduced in the presence of males, which produce substantially more pollen than hermaphrodites. Accordingly, we examined the evolutionary stability of trioecy using an experiment in which we (a) indirectly manipulated selfing rates by altering plant densities and the frequency of males in a fully factorial manner across 20 experimental plots and (b) examined the effect of these manipulations on the frequency of the three sex phenotypes in the next generation of plants. In the parental generation, we measured the seed and pollen allocations of hermaphrodites and compared them with allocations by unisexual plants. In natural populations, females occurred at higher frequencies in denser patches, a finding consistent with our expectations. Under our experimental conditions, however, no combination of plant densities and male frequencies was associated with increased frequencies of females. Our results suggest that the factors that regulate female frequencies in trioecious populations of M. annua are independent of those regulating male frequencies (density), and that the stable co-existence of all three sex phenotypes within populations is unlikely.

Regional variation in sex ratios and sex allocation in androdioecious Mercurialis annua.

In androdioecious metapopulations, where males co-occur with hermaphrodites, the absence of males from certain populations or regions may be explained by locally high selfing rates, high hermaphrodite outcross siring success (e.g. due to high pollen production by hermaphrodites), or to stochastic processes (e.g. the failure of males to invade populations or regions following colonization or range expansion by hermaphrodites). In the Iberian Peninsula and Morocco, the presence of males with hermaphrodites in the wind-pollinated androdioecious plant Mercurialis annua (Euphorbiaceae) varies both among populations within relatively small regions and among regions, with some regions lacking males from all populations. The species is known to have expanded its range into the Iberian Peninsula from a southern refugium. To account for variation in male presence in M. annua, we test the following hypotheses: (1) that males are absent in areas where plant densities are lower, because selfing rates should be correspondingly higher; (2) that males are absent in areas where hermaphrodites produce more pollen; and (3) that males are absent in areas where there is an elevated proportion of populations in which plant density and hermaphrodite pollen production disfavour their invasion. We found support for predictions two and three in Morocco (the putative Pleistocene refugium for M. annua) but no support for any hypothesis in Iberia (the expanded range). Our results are partially consistent with a hypothesis of sex-allocation equilibrium for populations in Morocco; in Iberia, the absence of males from large geographical regions is more consistent with a model of sex-ratio evolution in a metapopulation with recurrent population turnover. Our study points to the role of both frequency-dependent selection and contingencies imposed by colonization during range expansions and in metapopulations.

'Tommy Atkins' mango trees subjected to high density planting in subhumid tropical climate in northeastern Brazil

The objective of this work was to evaluate the effects of high density planting on 'Tommy Atkins' mango trees cultivated in subhumid warm tropical climate in northeastern Brazil. Treatments consisted of five spacial arrangements of plants (8x5 m, 7x4 m, 6x3 m, 5x2 m and 4x2 m), which resulted in the following plant densities: 250 (control), 357, 555, 1,000 and 1,250 plants per hectare. Plant vegetative and reproductive variables, besides fruit quality parameters, were evaluated at seven and eight years after transplantation to the field. In general, high density planting caused reduction in vegetative and reproductive variables of individual mango trees, but had little influence on fruit quality. Above 555 plants per hectare, a significant decrease was observed in mango tree growth. Furthermore, there were decreases in the percentage of flowering, fruit yield per plant and per area. However, planting density up to 357 plants per hectare, in spite of decreasing plant growth and fruit yield per tree, increases fruit yield per area in 30% in comparison to the control.

Efficiency of water use in sunflower grown in hydroponic system under saline stress

In order to identify alternatives for the use of saline water in agricultural production, the effects of the use of brackish water in the preparation of the nutrient solution for the cultivation of sunflower (cv. EMBRAPA 122-V2000) were studied in hydroponic system on consumption and efficiency of water use for the production of achenes and biomass. A completely randomized design was used, analyzed in a 5x2 factorial scheme with three replications. The factors studied were five levels of salinity of nutrient solution (1.7 - control; 4.3; 6.0; 9.0; and 11.5dS m-1) and two plant densities - one or two plants per vessel. It was concluded that the water consumption of sunflower is a variable sensitive to the salinity of the nutrient solution, especially after the fourth week of crop, and that the efficiency of water use in the production of achenes and biomass of sunflower is greater when the plant density increases from one to two plants per vessel, even under saline stress.

Effects of weed species competition on the growth of young coffee plants

The effects of competition of seven weed species on the growth of coffee plants were evaluated under greenhouse conditions. Thirty days after coffee seedling transplantation into 12 L pots with soil level area of 6.5 dm², weeds were transplanted into or sown in those pots, at densities of 0, 1, 2, 3, 4 and 5 plants per pot. Competition or weedy periods from weed transplantation or emergence to plant harvesting, at weed pre-flowering stage, were: 77 days - Bidens pilosa, 98 days - Brachiaria decumbens, 180 days - Commelina diffusa, 82 days - Leonurus sibiricus, 68 days - Nicandra physaloides, 148 days - Richardia brasiliensis and 133 days - Sida rhombifolia. Coffee plant height, stem diameter, leaf number and shoot dry matter were determined. Effects of competition by N. physaloides and S. rhombifolia against coffee plants were among the lowest, since only a slight decrease in all the characteristics evaluated in coffee plants was observed. The other weed species caused severe decrease in growth, mainly with increasing weed plant densities. Competition degree was found to depend on weed species and density.

The influence of winter oilseed rape (Brassica napus ssp oleifera var. biennis) canopy size on grass weed growth and grass weed seed return

Four experiments conducted over three seasons (2002-05) at the Crops Research Unit, University of Reading, investigated effects of canopy management of autumn sown oilseed rape (Brassica napus L. ssp. oleifera var. biennis (DC.) Metzg.) on competition with grass weeds. Emphasis was placed on the effect of the crop on the weeds. Rape canopy size was manipulated using sowing date, seed rate and the application of autumn fertilizer. Lolium multiflorum Lam., L. x boucheanum Kunth and Alopecurus myosuroides Huds. were sown as indicative grass weeds. The effects of sowing date, seed rate and autumn nitrogen on crop competitive ability were correlated with rape biomass and fractional interception of photosynthetically active radiation (PAR) by the rape floral layer, to the extent that by spring there was good evidence of crop: weed replacement. An increase in seed rate up to the highest plant densities tested increased both rape biomass and competitiveness, e.g. in 2002/3, L. multiflorum head density was reduced from 539 to 245 heads/m(2) and spikelet density from 13 170 to 5960 spikelets/m(2) when rape plant density was increased from 16 to 81 plants/m(2). Spikelets/head of Lolium spp. was little affected by rape seed rate, but the length of heads of A. myosuroides was reduced by 9 % when plant density was increased from 29-51 plants/m(2). Autumn nitrogen increased rape biomass and reduced L. multiflorum head density (415 and 336 heads/m(2) without and with autumn nitrogen, respectively) and spikelet density (9990 and 8220 spikelets/m(2) without and with autumn nitrogen, respectively). The number of spikelets/head was not significantly affected by autumn nitrogen. Early sowing could increase biomass and competitiveness, but poor crop establishment sometimes overrode the effect. Where crop and weed establishment was similar for both sowing dates, a 2-week delay (i.e. early September to mid-September) increased L. multiflorum head density from 226 to 633 heads/m(2) and spikelet density from 5780 to 15 060 spikelets/m(2).

The influence of winter oilseed rape (Brassica napus ssp. oleifera var. biennis) cultivar and grass genotype on the competitive balance between crop and grass weeds

Four experiments conducted over three seasons (2002–05) at the Crops Research Unit, University of Reading, investigated effects of canopy management of autumn sown oilseed rape (Brassica napus L. ssp. oleifera var. biennis (DC.) Metzg.) on competition with grass weeds. Emphasis was placed on the effect of the crop on the weeds. Rape canopy size was manipulated using sowing date, seed rate and the application of autumn fertilizer. Lolium multiflorum Lam., L.rboucheanum Kunth and Alopecurus myosuroides Huds. were sown as indicative grass weeds. The effects of sowing date, seed rate and autumn nitrogen on crop competitive ability were correlated with rape biomass and fractional interception of photosynthetically active radiation (PAR) by the rape floral layer, to the extent that by spring there was good evidence of crop: weed replacement. An increase in seed rate up to the highest plant densities tested increased both rape biomass and competitiveness, e.g. in 2002/3, L. multiflorum head density was reduced from 539 to 245 heads/m2 and spikelet density from 13 170 to 5960 spikelets/m2 when rape plant density was increased from 16 to 81 plants/m2. Spikelets/head of Lolium spp. was little affected by rape seed rate, but the length of heads of A. myosuroides was reduced by 9%when plant density was increased from 29–51 plants/m2. Autumn nitrogen increased rape biomass and reduced L. multiflorum head density (415 and 336 heads/m2 without and with autumn nitrogen, respectively) and spikelet density (9990 and 8220 spikelets/m2 without and with autumn nitrogen, respectively). The number of spikelets/head was not significantly affected by autumn nitrogen. Early sowing could increase biomass and competitiveness, but poor crop establishment sometimes overrode the effect. Where crop and weed establishment was similar for both sowing dates, a 2-week delay (i.e. early September to mid-September) increased L. multiflorum head density from 226 to 633 heads/m2 and spikelet density from 5780 to 15 060 spikelets/m2.

The influence of winter oilseed rape (Brassica napus ssp oleifera var. biennis) canopy size on grass weed growth and grass weed seed return

Four experiments conducted over three seasons (2002-05) at the Crops Research Unit, University of Reading, investigated effects of canopy management of autumn sown oilseed rape (Brassica napus L. ssp. oleifera var. biennis (DC.) Metzg.) on competition with grass weeds. Emphasis was placed on the effect of the crop on the weeds. Rape canopy size was manipulated using sowing date, seed rate and the application of autumn fertilizer. Lolium multiflorum Lam., L. x boucheanum Kunth and Alopecurus myosuroides Huds. were sown as indicative grass weeds. The effects of sowing date, seed rate and autumn nitrogen on crop competitive ability were correlated with rape biomass and fractional interception of photosynthetically active radiation (PAR) by the rape floral layer, to the extent that by spring there was good evidence of crop: weed replacement. An increase in seed rate up to the highest plant densities tested increased both rape biomass and competitiveness, e.g. in 2002/3, L. multiflorum head density was reduced from 539 to 245 heads/m(2) and spikelet density from 13 170 to 5960 spikelets/m(2) when rape plant density was increased from 16 to 81 plants/m(2). Spikelets/head of Lolium spp. was little affected by rape seed rate, but the length of heads of A. myosuroides was reduced by 9 % when plant density was increased from 29-51 plants/m(2). Autumn nitrogen increased rape biomass and reduced L. multiflorum head density (415 and 336 heads/m(2) without and with autumn nitrogen, respectively) and spikelet density (9990 and 8220 spikelets/m(2) without and with autumn nitrogen, respectively). The number of spikelets/head was not significantly affected by autumn nitrogen. Early sowing could increase biomass and competitiveness, but poor crop establishment sometimes overrode the effect. Where crop and weed establishment was similar for both sowing dates, a 2-week delay (i.e. early September to mid-September) increased L. multiflorum head density from 226 to 633 heads/m(2) and spikelet density from 5780 to 15 060 spikelets/m(2).

Produção e qualidade fisiológica de sementes de soja avaliadas na semeadura de inverno

O cultivo da soja fora da época convencional pode ser uma alternativa de rotação de cultura além de proporcionar sementes de melhor qualidade fisiológica, que podem ser utilizadas na semeadura da próxima safra diminuindo o período de armazenamento. O trabalho de pesquisa teve por objetivo avaliar o comportamento de sete cultivares de soja, em três densidades populacionais, quanto ao porte e altura de inserção da primeira vagem, assim como quanto à produção e qualidade fisiológica das sementes, semeadas no período de inverno, na região de Selvíria-MS. O delineamento experimental foi em blocos casualizados, com quatro repetições, dispostos em esquema fatorial 7 ×3. Os tratamentos constaram de sete cultivares (IAC-16, IAC-Foscarin 31, FT-2, IAC-17, IAC-8, Doko e FT-Cristalina) e três densidades de plantas (300, 400 e 500 mil plantas ha-1). O ciclo da soja foi reduzido no cultivo de inverno, principalmente nos cultivares considerados tardios. O fotoperíodo no cultivo de inverno reduziu o período entre o florescimento e a maturação. O cultivar IAC-8 foi o menos sensível ao fotoperíodo, possuindo maturação em época semelhante aos cultivares tardios, e apresentou as melhores características agronômicas para o cultivo no período de inverno. É aconselhável o aumento de densidade de semeadura quando do uso de cultivares precoces em cultivos de inverno. Não é aconselhável armazenar sementes em condições ambientes, principalmente com valores de germinação próximo ao limite inferior desejável (80%).

Influencia de las malezas sobre el cultivo de frijol en función de espaciamiento y de la densidad de plantas

La hipótesis de investigación es que con la reducción de la densidad de siembra y del espaciamiento entre líneas, el período anterior a la interferencia de las malezas será reducido en el cultivo de frijol. El objetivo de este trabajo fue determinar el período anterior a la interferencia de las malezas (PAI) en función del espaciamiento entre líneas y de su densidad poblacional. Los tratamientos fueron constituidos de ocho períodos de convivencia del cultivo con las malezas: 0-10, 0-20, 0-30, 0-40, 0-50, 0-60, 0-70 y 0-80 días después de la emergencia y un control libre de interferencia. Los períodos de convivencia fueron aplicados en dos experimentos, utilizando dos distancias entre líneas, de 0,45 y 0,60 m y en dos densidades de plantas por línea de siembra, de 10 y 15 plantas por metro. El delineamiento experimental utilizado fue en bloques completos al azar, con cuatro repeticiones. Hubo reducción de 16, 40, 36 y 58% en la productividad de granos del cultivo de frijol cuando convivió durante todo el ciclo del cultivo con las malezas, para el espaciamiento de 0,45 m en las densidades de 10 y 15 plantas m-1; y espaciamiento de 0,60 m y densidades de 10 y 15 plantas m-1, respectivamente. La productividad de granos pasó a ser afectada negativamente a partir de 28, 26, 22 y 14 días después de la emergencia, constituyéndose en los períodos anteriores a la interferencia del cultivo, respectivamente.

Adensamento da beterraba no manejo de plantas daninhas

O objetivo deste trabalho foi verificar o efeito do adensamento da semeadura na capacidade de supressão da cultura da beterraba sobre a comunidade infestante. Os tratamentos constituíram-se de 12 períodos semanais crescentes de convivência e controle das plantas daninhas, a partir da segunda semana após a semeadura, submetidos a duas densidades populacionais da cultura (40 e 50 plantas m-2). Avaliou-se o acúmulo de massa seca pelas plantas daninhas em cada período e a estimativa da época e extensão dos períodos críticos de interferência das plantas daninhas em função da produtividade comercial da cultura. Observou-se menor acúmulo de massa seca pelas plantas daninhas quando a cultura foi adensada, sendo a diferença média no acúmulo de 17 e 30% para períodos de convivência e controle, respectivamente. O período crítico de prevenção à interferência foi menor na cultura adensada (11 dias) em relação à não-adensada (22 dias). O adensamento de semeadura da cultura da beterraba proporcionou aumento na capacidade de supressão da cultura sobre a comunidade infestante, afetando o crescimento e a época e extensão dos períodos críticos de interferência das plantas daninhas, podendo ser usado como ferramenta eficaz no manejo da flora invasora.

Fertilização nitrogenada, densidade de plantas e rendimento de milho cultivado no sistema plantio direto

In maize (Zea mays L.), the inadequate nitrogen supply and planting density are considered limiting factors to the grain yield. The objective of this study was to evaluate the influence of different nitrogen levels and plant densities to the maize yield components and productivity. The experiment was carried out in the Cerrado region, located in the southern of the Maranhao State, Brazil, in a clay Oxisol (Ustox), cropped under the no-tillage system for six years. The randomized completed blocks experimental design with four replications was used, with treatments arrangement in a 5 x 4 factorial. The treatments were a combination of five doses of nitrogen as urea (0, 50, 100, 150 and 200 kg ha(-1)) and four plant densities (25,000; 50,000; 75,000 and 100,000 plants he). The grain number and mass per spike and grain mass per plant were improved by increased N and decreased plant density. Higher incomes of grains were obtained with adding concomitantly in N doses and in plants density. The maximum grain yield (11,9 Mg ha(-1)) was obtained with 120 kg ha(-1) of N and 83,000 plants ha(-1).

Effects of different soil tillage systems and coverages on soybean crop in the Botucatu Region in Brazil

Nowadays, agricultural practices should combine high yields with a sustainable use of resources. Different tillage practices and crop covers, if combined, may help to achieve both objectives. In this work, several traits of a soybean (Glycine max L. Merr) cultivar were studied under different conditions of tillage and previous soil coverages. The experiment was installed at Lageado Research Station, Botucatu county, SP, Brazil, on a Paleudult. It consisted of nine treatments (combining three systems of soil tillage and three cover crops) and 4 replicates, yielding 36 plots of a randomized block experimental design. The soil tillage systems considered were: (i) conventional tillage with two heavy harrowing and a levelling harrowing; (ii) chiseling, and (iii) no-tillage with chemical drying of vegetation. The three cover crops used were: black oat, sorghum and spontaneous vegetation. Analyzed variables were: plant height, initial and final plant densities, height of first pod insertion, weight of a thousand grains, number of pods per plant, number of grains per pod, and crop yield. No significant differences were observed for most of the analyzed variables; however, conventional tillage produced significantly heavier grains and a higher number of pods per plant. The selected covers were considered an excellent coverage prior to planting soybean in a crop rotation. The three tillage systems can be used for deployment of culture without compromising the development of soybean.

População de plantas, método de colheita e qualidade de sementes de cenoura, cultivar Brasília

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)