Difference variance dispersion graphs for comparing response surface designs with applications in food technology

Variance dispersion graphs have become a popular tool in aiding the choice of a response surface design. Often differences in response from some particular point, such as the expected position of the optimum or standard operating conditions, are more important than the response itself. We describe two examples from food technology. In the first, an experiment was conducted to find the levels of three factors which optimized the yield of valuable products enzymatically synthesized from sugars and to discover how the yield changed as the levels of the factors were changed from the optimum. In the second example, an experiment was conducted on a mixing process for pastry dough to discover how three factors affected a number of properties of the pastry, with a view to using these factors to control the process. We introduce the difference variance dispersion graph (DVDG) to help in the choice of a design in these circumstances. The DVDG for blocked designs is developed and the examples are used to show how the DVDG can be used in practice. In both examples a design was chosen by using the DVDG, as well as other properties, and the experiments were conducted and produced results that were useful to the experimenters. In both cases the conclusions were drawn partly by comparing responses at different points on the response surface.

A Semi-Continuous Analyzer for the Fluorimetric Determination of Atmospheric Formaldehyde

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Otimização do cultivo de Lasiodiplodia theobromae para obtenção de EPS e caracterização física e química dos polissacarídeos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Electrochemical oxidation of landfill leachate in a flow reactor: optimization using response surface methodology

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Microparticulated Hydrochlorothiazide Solid Dispersion: Enhancing Dissolution Properties via Spray Drying

The aim of the present study was to obtain microparticles of hydrochlorothiazide, a diuretic drug that practically insoluble in water, by spray drying and to investigate the influence of process parameters using a three-level, three-factor Box-Behnken design. Process yields, moisture content, particle size, flowability, and solubility were used to evaluate the spray-dried microparticles. The data were analyzed by response surface methodology using analysis of variance. The independent variables studied were outlet temperature, atomization pressure, and drug content. The formulations were prepared using polyvinylpyrrolidone and colloidal silicon dioxide as the hydrophilic carrier and drying aid, respectively. The microparticle yield ranged from 18.15 to 59.02% and resulted in adequate flow (17 to 32 degrees), moisture content between 2.52 to 6.18%, and mean particle size from 45 to 59 mu m. The analysis of variance showed that the factors studied influenced the yields, moisture content, angle of repose, and solubility. Thermal analysis and X-ray diffractometry evidenced no drug interactions or chemical modifications. Photomicrographs obtained by scanning electron microscopy showed spherical particles. The solubility and dissolution rates of hydrochlorothiazide were remarkably improved when compared with pure drug. Therefore, the results confirmed the high potential of the spray-drying technique to obtain microparticulate hydrochlorothiazide with enhanced pharmaceutical and dissolution properties.

Valutazione delle condizioni di estrazione nella determinazione di composti fenolici nelle patate

Il presente elaborato di tesi valuta l’effetto delle condizioni di estrazione dei composti fenolici in un sottoprodotto della lavorazione industriale delle patate, la buccia, con l’obiettivo di poter valorizzare tale residuo di processo per utilizzi futuri. Al fine di ottimizzare le condizioni di estrazione, si è deciso di utilizzare il disegno sperimentale Box-Behnken (DBB) mediante la variazione di tre fattori (tempo, tipologia di solvente, rapporto campione/solvente) e per un totale di 15 determinazioni. I dati risultanti dalla determinazione dei composti fenolici totali e dell’attività antiossidante hanno messo in evidenza come la buccia di patate, sottoprodotto del processo di lavorazione industriale, sia un prodotto interessante per il proprio contenuto in composti fenolici ad elevata attività antiossidante. Tuttavia, la scelta delle condizioni ottimali di estrazione è fondamentale al fine di evitare la sottostima del contenuto in composti bioattivi, che deriverebbe da una incompleta estrazione degli stessi dalla matrice alimentare. Il disegno Box-Behnken ha permesso di stabilire le condizioni ottimali di estrazione dei composti fenolici e per questo sará utilizzato in futuro per lo screening di diversi campioni procedenti dalla produzione industriale. L’estrazione ottimale dei composti di interesse ha comunque evidenziato come la buccia di patata sia una fonte di composti fenolici ad attivitá antiossidante che potrá essere utilizzata sia come ingrediente alimentare che in campo nutraceutico.

Optimization of microwave-assisted extration of phenolic compounds from tomao by full factorial design coupled with response surface methodology

Tomato (Lycopersicon esculentum Mill.), apart from being a functional food rich in carotenoids, vitamins and minerals, is also an important source of phenolic compounds [1 ,2]. As antioxidants, these functional molecules play an important role in the prevention of human pathologies and have many applications in nutraceutical, pharmaceutical and cosmeceutical industries. Therefore, the recovery of added-value phenolic compounds from natural sources, such as tomato surplus or industrial by-products, is highly desirable. Herein, the microwave-assisted extraction of the main phenolic acids and flavonoids from tomato was optimized. A S-Ieve! full factorial Box-Behnken design was implemented and response surface methodology used for analysis. The extraction time (0-20 min), temperature (60-180 "C), ethanol percentage (0-100%), solidlliquid ratio (5-45 g/L) and microwave power (0-400 W) were studied as independent variables. The phenolic profile of the studied tomato variety was initially characterized by HPLC-DAD-ESIIMS [2]. Then, the effect of the different extraction conditions, as defined by the used experimental design, on the target compounds was monitored by HPLC-DAD, using their UV spectra and retention time for identification and a series of calibrations based on external standards for quantification. The proposed model was successfully implemented and statistically validated. The microwave power had no effect on the extraction process. Comparing with the optimal extraction conditions for flavonoids, which demanded a short processing time (2 min), a low temperature (60 "C) and solidlliquid ratio (5 g/L), and pure ethanol, phenolic acids required a longer processing time ( 4.38 min), a higher temperature (145.6 •c) and solidlliquid ratio (45 g/L), and water as extraction solvent. Additionally, the studied tomato variety was highlighted as a source of added-value phenolic acids and flavonoids.

Microwave-assisted extraction of phenolic acids and flavonoids and production of antioxidant ingredients from tomato: a nutraceutical-oriented optimization study

The production of natural extracts requires suitable processing conditions to maximize the preservation of the bioactive ingredients. Herein, a microwave-assisted extraction (MAE) process was optimized, by means of response surface methodology (RSM), to maximize the recovery of phenolic acids and flavonoids and obtain antioxidant ingredients from tomato. A 5-level full factorial Box-Behnken design was successfully implemented for MAE optimization, in which the processing time (t), temperature (T), ethanol concentration (Et) and solid/liquid ratio (S/L) were relevant independent variables. The proposed model was validated based on the high values of the adjusted coefficient of determination and on the non-significant differences between experimental and predicted values. The global optimum processing conditions (t=20 min; T=180 ºC; Et=0 %; and S/L=45 g/L) provided tomato extracts with high potential as nutraceuticals or as active ingredients in the design of functional foods. Additionally, the round tomato variety was highlighted as a source of added-value phenolic acids and flavonoids.

Antitumor effect and mechanism of action of polysaccharides extracted from Polygonum perfoliatum L whole plant in human lung carcinoma A549 cell line

Purpose: To optimize the extraction conditions of polysaccharides from Polygonum perfoliatum L. (PSDP) and to evaluate their anti-tumor activities on A549 cell line. Methods: Extraction of PSDP was optimized using Box-Behnken design (BBD). Three factors of response surface methodology (RSM) including extraction time, ratio of water to raw material and number of extractions were employed to optimize the yield of PSDP. The cytotoxic effect of PSDP on human lung carcinoma A549 cell line was evaluated in vivo, while its effects on expressions of caspase3, caspase-9, Bcl-2 and Bax were determined by western blot assay. Result: BBD was significant and applicable to PSDP extraction. Based on the contour plots, response surface plots and variance analysis, it predicted that the optimum conditions for PSDP extraction were: 1.58 h (extraction time); 30.18 mL/g (ratio of water to raw material); and 2.02 (number of extractions). PSDP had significant inhibitory effect on the growth of A549 cells in a concentration- and timedependent manner (p < 0.05). After treatment with PSDP, caspase-3, caspase-9 and Bax were significantly up-regulated (p < 0.05), whereas Bcl-2 was down-regulated, all concentration-dependently. Conclusion: RSM analysis is an appropriate method to optimize PSDP extraction. The results also indicate that PSDP has significant anti-tumor effect against A549 cells, most likely via inducing mitochondria-mediated apoptosis.

Previsão estocástica de nível fluviométrico para cidade de Marabá-PA: método de Box-Jenkins

O Município de Marabá- PA, situado na região Amazônica, sudeste do Estado do Pará, sofre anualmente com eventos de enchentes, ocasionados pelo aumento periódico do rio Tocantins e pela situação de vulnerabilidade da população que reside em áreas de risco. A defesa civil estadual e municipal anualmente planeja e prepara equipes para ações de defesa no município. Nesta fase o monitoramento e previsão de eventos de enchentes são importantes. Portanto, com o objetivo de diminuir erros nas previsões hidrológicas para o Município de Marabá, desenvolveu-se um modelo estocástico para previsão de nível do rio Tocantins, baseado na metodologia de Box e Jenkins. Utilizou os dados de níveis diários observados nas estações hidrológicas de Marabá e Carolina e Conceição do Araguaia da Agência Nacional de Águas (ANA), do período de 01/12/ 2008 a 31/03/2011. Efetuou-se o ajustamento de três modelos (M_t, N_t e Y_t), através de diferentes aplicativos estatísticos: o SAS e o Gretl, usando diferentes interpretações do comportamento das séries para gerar as equações dos modelos. A principal diferença entre os aplicativos é que no SAS usa o modelo de função de transferência na modelagem. Realizou-se uma classificação da variabilidade do nível do rio, através da técnica dos Quantis para o período de 1972 a 2011, examinando-se apenas as categorizações de níveis ACIMA e MUITO ACIMA do normal. Para análise de impactos socioeconômicos foram usados os dados das ações da Defesa Civil Estado do Pará nas cheias de 2009 e 2011. Os resultados mostraram que o número de eventos de cheias com níveis MUITO ACIMA do normal, geralmente, podem estar associados a eventos de La Niña. Outro resultado importante: os modelos gerados simularam muito bem o nível do rio para o período de sete dias (01/04/2011 a 07/04/2011). O modelo multivariado N_t (com pequenos erros) representou o comportamento da série original, subestimando os valores reais nos dias 3, 4 e 5 de abril de 2011, com erro máximo de 0,28 no dia 4. O modelo univariado (Y_t) teve bons resultados nas simulações com erros absolutos em torno de 0,12 m. O modelo com menor erro absoluto (0,08m) para o mesmo período foi o modelo M_t, desenvolvido pelo aplicativo SAS, que interpreta a série original como sendo não linear e não estacionária. A análise quantitativa dos impactos fluviométricos, ocorridos nas enchentes de 2009 e 2011 na cidade de Marabá, revelou em média que mais de 4 mil famílias sofrem com estes eventos, implicado em gastos financeiros elevados. Logo, conclui-se que os modelos de previsão de níveis são importantes ferramentas que a Defesa Civil, utiliza no planejamento e preparo de ações preventivas para o município de Marabá.

Sound radiation characteristics of a box-type structure

The finite element and boundary element methods are employed in this study to investigate the sound radiation characteristics of a box-type structure. It has been shown [T.R. Lin, J. Pan, Vibration characteristics of a box-type structure, Journal of Vibration and Acoustics, Transactions of ASME 131 (2009) 031004-1–031004-9] that modes of natural vibration of a box-type structure can be classified into six groups according to the symmetry properties of the three panel pairs forming the box. In this paper, we demonstrate that such properties also reveal information about sound radiation effectiveness of each group of modes. The changes of radiation efficiencies and directivity patterns with the wavenumber ratio (the ratio between the acoustic and the plate bending wavenumbers) are examined for typical modes from each group. Similar characteristics of modal radiation efficiencies between a box structure and a corresponding simply supported panel are observed. The change of sound radiation patterns as a function of the wavenumber ratio is also illustrated. It is found that the sound radiation directivity of each box mode can be correlated to that of elementary sound sources (monopole, dipole, etc.) at frequencies well below the critical frequency of the plates of the box. The sound radiation pattern on the box surface also closely related to the vibration amplitude distribution of the box structure at frequencies above the critical frequency. In the medium frequency range, the radiated sound field is dominated by the edge vibration pattern of the box. The radiation efficiency of all box modes reaches a peak at frequencies above the critical frequency, and gradually approaches unity at higher frequencies.

Mobiles In-a-Box : Reflections on Developing a Toolkit with Grassroots Human Rights Advocates

In 2008 Tactical Tech published 'Mobiles in-a-box': a toolkit designed to help human rights organisations and advocates use mobile technology in their work in Africa. This chapter reflects on the participatory development process used to develop the toolkit.

Investigating international accounting standard setting : the black box of IFRS 6

This paper examines the role of powerful entities and coalitions in shaping international accounting standards. Specifically, the focus is on the process by which the International Accounting Standards Board (IASB) developed IFRS 6, Exploration for and Evaluation of Mineral Resources. In its Issues Paper, the IASB recommended that the successful efforts method be mandated for pre-production costs, eliminating the choice previously available between full cost and successful efforts methods. In spite of the endorsement of this view by a majority of the constituents who responded to the Issues Paper, the final outcome changed nothing, with choice being retained. A compelling explanation of this disparity between the visible inputs and outputs of the standard setting process is the existence of a “black box”, in which powerful extractive industries entities and coalitions covertly influenced the IASB to secure their own ends and ensure that the status quo was maintained