Dr. Sevier,

Mode of access: Internet.

The sugar-beet industry. Culture of the sugar-beet and manufacture of beet sugar.

Published by authority of the Secretary of Agriculture.

The southern struggle for pure government. An address.

Printed wrappers.

The voyage of the Jeannette. : The ship and ice journals of George W. De Long, lieutenant-commander U.S.N. and commander of the Polar expedition of 1879-1881 /

Paged continuously: XII, 440 p.; XII, [441]-911 p.

Three years of Arctic service; an account of the Lady Franklin Bay Expedition of 1881-84, and the attainment of the farthest north,

Mode of access: Internet.

Mazzini's letters to an English family.

The notes signed E. A. V. are those left by Madame Venturi, who for some years before her death had been engaged upon copying and annotating the letters.

Characterization of a Full-Length TTP Family Member Association with RNA Sequence Elements

Post-transcriptional regulation of cytoplasmic mRNAs is an efficient mechanism of regulating the amounts of active protein within a eukaryotic cell. RNA sequence elements located in the untranslated regions of mRNAs can influence transcript degradation or translation through associations with RNA-binding proteins. Tristetraprolin (TTP) is the best known member of a family of CCCH zinc finger proteins that targets adenosine-uridine rich element (ARE) binding sites in the 3’ untranslated regions (UTRs) of mRNAs, promoting transcript deadenylation through the recruitment of deadenylases. More specifically, TTP has been shown to bind AREs located in the 3’-UTRs of transcripts with known roles in the inflammatory response. The mRNA-binding region of the protein is the highly conserved CCCH tandem zinc finger (TZF) domain. The synthetic TTP TZF domain has been shown to bind with high affinity to the 13-mer sequence of UUUUAUUUAUUUU. However, the binding affinities of full-length TTP family members to the same sequence and its variants are unknown. Furthermore, the distance needed between two overlapping or neighboring UUAUUUAUU 9-mers for tandem binding events of a full-length TTP family member to a target transcript has not been explored. To address these questions, we recombinantly expressed and purified the full-length C. albicans TTP family member Zfs1. Using full-length Zfs1, tagged at the N-terminus with maltose binding protein (MBP), we determined the binding affinities of the protein to the optimal TTP binding sequence, UUAUUUAUU. Fluorescence anisotropy experiments determined that the binding affinities of MBP-Zfs1 to non-canonical AREs were influenced by ionic buffer strength, suggesting that transcript selectivity may be affected by intracellular conditions. Furthermore, electrophoretic mobility shift assays (EMSAs) revealed that separation of two core AUUUA sequences by two uridines is sufficient for tandem binding of MBP-Zfs1. Finally, we found evidence for tandem binding of MBP-Zfs1 to a 27-base RNA oligonucleotide containing only a single ARE-binding site, and showed that this was concentration and RNA length dependent; this phenomenon had not been seen previously. These data suggest that the association of the TTP TZF domain and the TZF domains of other species, to ARE-binding sites is highly conserved. Domains outside of the TZF domain may mediate transcript selectivity in changing cellular conditions, and promote protein-RNA interactions not associated with the ARE-binding TZF domain.

In summary, the evidence presented here suggests that Zfs1-mediated decay of mRNA targets may require additional interactions, in addition to ARE-TZF domain associations, to promote transcript destabilization and degradation. These studies further our understanding of post-transcriptional steps in gene regulation.

Los políticos del progreso. Científicos en el gobierno y en el Partido Progresista (1833-1868)

La organización del Partido Progresista tiene una configuración abierta, que ensaya fórmulas de elecciones primarias y que con contará con dos núcleos: La minoría parlamentaria y una organización que será permanente desde 1846, en la que se distingue la Junta Central y la Comisión Directiva. Entre los políticos de la generación del progreso Francisco de Luxán será el primero de una nómina muy reducida de ministros con formación científico-técnica, y entre los dirigentes del Partido Progresista y de la Unión Liberal será también el único con formación científico-técnica.

Narrative of the expedition of an American squadron to the China Seas and Japan : performed in the years 1852, 1853, and 1854, under the command of Commodore M.C. Perry, United States Navy, by order of the government of the United States /

Includes indexes.

Puget Sound, Washington, 1854 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Reconnaissance of Duwamish Bay & Seattle Harbor, Washington Ter., by the hydrographic party under the command of Lieut. James Alden, U.S.N. Assistant; redd. drng by J. R. Key; engd. by Apps. J.J. Knight & F. W. Benner. It was published by the U.S. Coast Survey in 1854. Scale 1:40,000. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Washington State Plane North Coordinate System HARN NAD83 (in Feet) (Fipszone 4601). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows coastal features such as channels, points, coves, islands, bottom soil types, flats, and more. Depths shown by soundings. Includes note. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Public laws of the state of Rhode-Island and Providence plantations, as revised by a committee, and finally enacted by the General assembly at the session in January, 1844. To which are prefixed, the Charter of Charles II., Declaration of independence, resolution of General assembly to support the Declaration of independence, resolution of General assembly to support the Declaration of independence, Articles of confederation, Constitution of the United States, proceedings of the Convention on the adoption of the Constitution of the United States by Rhode-Island, President Washington's address of September, 1796, and constitution of the state of Rhode-Island and Providence plantations.

Mode of access: Internet.

Encyclopédie théologique; ou, Série de dictionnaires sur toutes les parties de la science religieuse ... t. 1-50, 1844-1862; nouv, sér. t. 1-52, 1851-1866; 3e sér. t. 1-66, 1854-1873.

Title varies: Nouv. sér., Nouvelle encyclopédie théologique; 3e sér., Troisième et dernière encyclopédie théologique.

Perry Syndrome

Disease characteristics. Perry syndrome is characterized by parkinsonism, hypoventilation, depression, and weight loss. The mean age at onset is 48 years; the mean disease duration is five years. Parkinsonism and psychiatric changes (depression, apathy, character changes, and withdrawal) tend to occur early; severe weight loss and hypoventilation manifest later. Diagnosis/testing. The diagnosis is based on clinical findings and molecular genetic testing of DCTN1, the only gene known to be associated with Perry syndrome. Management. Treatment of manifestations: Dopaminergic therapy (particularly levodopa/carbidopa) should be considered in all individuals with significant parkinsonism. Although response to levodopa is often poor, some individuals may have long-term benefit. Noninvasive or invasive ventilation support may improve quality of life and prolong life expectancy. Those patients with psychiatric manifestations may benefit from antidepressants and psychiatric care. Weight loss is managed with appropriate dietary changes. Surveillance: routine evaluation of weight and calorie intake, respiratory function (particularly at night or during sleep), strength; and mood. Agents/circumstances to avoid: Central respiratory depressants (e.g., benzodiazepines, alcohol). Genetic counseling. Perry syndrome is inherited in an autosomal dominant manner. The proportion of cases attributed to de novo mutations is unknown. Each child of an individual with Perry syndrome has a 50% chance of inheriting the mutation. No laboratories offering molecular genetic testing for prenatal diagnosis are listed in the GeneTests Laboratory Directory; however, prenatal testing may be available through laboratories offering custom prenatal testing for families in which the disease-causing mutation has been identified.